Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20797
(738 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q6P2Q9 Cluster: Pre-mRNA-processing-splicing factor 8; ... 177 2e-43
UniRef50_A6R4K4 Cluster: Pre-mRNA processing splicing factor 8; ... 150 3e-35
UniRef50_Q8I1X5 Cluster: Pre-mRNA splicing factor, putative; n=1... 136 7e-31
UniRef50_A4IBT3 Cluster: PRP8 protein homologue, putative; n=8; ... 96 9e-19
UniRef50_Q9AW36 Cluster: Splicing factor Prp8; n=1; Guillardia t... 58 3e-07
UniRef50_Q7QTZ9 Cluster: GLP_169_4270_455; n=1; Giardia lamblia ... 55 2e-06
UniRef50_Q8I5E6 Cluster: Putative uncharacterized protein; n=6; ... 36 0.78
UniRef50_UPI00006CE62B Cluster: hypothetical protein TTHERM_0070... 34 4.2
UniRef50_A4SB03 Cluster: Predicted protein; n=1; Ostreococcus lu... 34 4.2
UniRef50_O17517 Cluster: Putative uncharacterized protein; n=1; ... 34 4.2
UniRef50_Q5QCH6 Cluster: Maturase; n=2; Rhizanthes|Rep: Maturase... 33 7.3
UniRef50_A3DFA7 Cluster: Spore coat assembly protein-like protei... 33 9.7
UniRef50_A2DU71 Cluster: Putative uncharacterized protein; n=1; ... 33 9.7
UniRef50_O67554 Cluster: Uncharacterized kamA family protein aq_... 33 9.7
UniRef50_Q9PJP6 Cluster: ADP,ATP carrier protein 2; n=8; Chlamyd... 33 9.7
>UniRef50_Q6P2Q9 Cluster: Pre-mRNA-processing-splicing factor 8;
n=106; Eukaryota|Rep: Pre-mRNA-processing-splicing factor
8 - Homo sapiens (Human)
Length = 2335
Score = 177 bits (431), Expect = 2e-43
Identities = 77/85 (90%), Positives = 82/85 (96%)
Frame = +2
Query: 254 LMKHDVNLGRAVFWDIKNRLPRSVTTIQWENSFVSVYSKDNPNLLFNMAGFECRILPKCR 433
LMKHDVNLGRAVFWDIKNRLPRSVTT+QWENSFVSVYSKDNPNLLFNM GFECRILPKCR
Sbjct: 1142 LMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCR 1201
Query: 434 SQHEELSHRDGVWNLQNEVTKERTA 508
+ +EE +H+DGVWNLQNEVTKERTA
Sbjct: 1202 TSYEEFTHKDGVWNLQNEVTKERTA 1226
Score = 176 bits (429), Expect = 4e-43
Identities = 76/84 (90%), Positives = 81/84 (96%)
Frame = +3
Query: 3 ASEMAGPPQLPNDFLSYQDRQTETAHPIRLYCRYIDRIHIFFRFSAEEARDLIQRYLTEH 182
ASEMAGPPQ+PNDFLS+QD TE AHPIRL+CRYIDRIHIFFRF+A+EARDLIQRYLTEH
Sbjct: 1058 ASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEH 1117
Query: 183 PDPNNENIVGYNNKKCWPRDARMR 254
PDPNNENIVGYNNKKCWPRDARMR
Sbjct: 1118 PDPNNENIVGYNNKKCWPRDARMR 1141
Score = 155 bits (377), Expect = 8e-37
Identities = 73/78 (93%), Positives = 77/78 (98%)
Frame = +1
Query: 505 SKCYLRVDDESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELL 684
++C+LRVDDES+ RFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELL
Sbjct: 1226 AQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELL 1285
Query: 685 DLLVKCENKIQTRIKIGL 738
DLLVKCENKIQTRIKIGL
Sbjct: 1286 DLLVKCENKIQTRIKIGL 1303
>UniRef50_A6R4K4 Cluster: Pre-mRNA processing splicing factor 8; n=1;
Ajellomyces capsulatus NAm1|Rep: Pre-mRNA processing
splicing factor 8 - Ajellomyces capsulatus NAm1
Length = 2739
Score = 150 bits (364), Expect = 3e-35
Identities = 63/84 (75%), Positives = 74/84 (88%)
Frame = +3
Query: 3 ASEMAGPPQLPNDFLSYQDRQTETAHPIRLYCRYIDRIHIFFRFSAEEARDLIQRYLTEH 182
ASE+AGPPQ PNDFL ++DR TET HPIRLY RYID+I +F RFSA+E+RDLIQR+LTE
Sbjct: 967 ASEIAGPPQSPNDFLQFKDRSTETRHPIRLYTRYIDKIWVFLRFSADESRDLIQRFLTEQ 1026
Query: 183 PDPNNENIVGYNNKKCWPRDARMR 254
PDPN EN++GY NKKCWPRD+RMR
Sbjct: 1027 PDPNFENVIGYRNKKCWPRDSRMR 1050
Score = 143 bits (346), Expect = 5e-33
Identities = 61/87 (70%), Positives = 75/87 (86%)
Frame = +2
Query: 254 LMKHDVNLGRAVFWDIKNRLPRSVTTIQWENSFVSVYSKDNPNLLFNMAGFECRILPKCR 433
LM+HDVNLGRAVFWD+KNRLPRS+TTI+W+++F SVYSKDNPN+LF+M GFE RILPK R
Sbjct: 1051 LMRHDVNLGRAVFWDLKNRLPRSITTIEWDDTFASVYSKDNPNMLFSMCGFEVRILPKIR 1110
Query: 434 SQHEELSHRDGVWNLQNEVTKERTANA 514
+Q++E +D VW+L N TKERTA A
Sbjct: 1111 NQNDEFQVKDSVWSLANNTTKERTAYA 1137
Score = 115 bits (277), Expect = 1e-24
Identities = 51/75 (68%), Positives = 64/75 (85%)
Frame = +1
Query: 514 YLRVDDESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLL 693
+L+V +E + +F+NR+RQILM+SGSTTFTKI NKWNT+LI L TY+REA V+T LLD +
Sbjct: 1138 FLQVTEEDIQKFNNRIRQILMSSGSTTFTKIANKWNTSLIALFTYYREAAVSTVNLLDTI 1197
Query: 694 VKCENKIQTRIKIGL 738
VKCE KIQTR+KIGL
Sbjct: 1198 VKCETKIQTRVKIGL 1212
>UniRef50_Q8I1X5 Cluster: Pre-mRNA splicing factor, putative; n=1;
Plasmodium falciparum 3D7|Rep: Pre-mRNA splicing factor,
putative - Plasmodium falciparum (isolate 3D7)
Length = 3136
Score = 136 bits (328), Expect = 7e-31
Identities = 63/78 (80%), Positives = 71/78 (91%)
Frame = +1
Query: 505 SKCYLRVDDESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELL 684
++ YL+V D S+ RF NRVRQILM+SGSTTFTKI NKWNT LIGLMTYFREAV++T+ELL
Sbjct: 1881 AEAYLKVSDNSMKRFENRVRQILMSSGSTTFTKIANKWNTTLIGLMTYFREAVLDTEELL 1940
Query: 685 DLLVKCENKIQTRIKIGL 738
DLLVKCENKIQTRIKIGL
Sbjct: 1941 DLLVKCENKIQTRIKIGL 1958
Score = 132 bits (320), Expect = 7e-30
Identities = 56/84 (66%), Positives = 70/84 (83%)
Frame = +3
Query: 3 ASEMAGPPQLPNDFLSYQDRQTETAHPIRLYCRYIDRIHIFFRFSAEEARDLIQRYLTEH 182
A ++AGP N FL++Q+ Q ET HPIRLYCRY+D+I I F+F+ EE++DLIQ++LTE+
Sbjct: 1605 AYDIAGPYNDVNQFLTFQNVQIETRHPIRLYCRYVDKIWILFKFTNEESKDLIQKFLTEN 1664
Query: 183 PDPNNENIVGYNNKKCWPRDARMR 254
PDPNNENIVGYNNK CWPRD RMR
Sbjct: 1665 PDPNNENIVGYNNKTCWPRDCRMR 1688
Score = 105 bits (251), Expect = 1e-21
Identities = 46/68 (67%), Positives = 57/68 (83%), Gaps = 2/68 (2%)
Frame = +2
Query: 236 KGCSYALMKHDVNLGRAVFWDIKNRLPRSVTTIQWE--NSFVSVYSKDNPNLLFNMAGFE 409
+ C MKHDVNLGRA FW+I+NR+PRS+T++ W+ N+FVSVYSKDNPNLLF++AGFE
Sbjct: 1683 RDCRMRKMKHDVNLGRATFWEIQNRIPRSLTSLDWDHYNTFVSVYSKDNPNLLFSIAGFE 1742
Query: 410 CRILPKCR 433
RILPK R
Sbjct: 1743 VRILPKIR 1750
>UniRef50_A4IBT3 Cluster: PRP8 protein homologue, putative; n=8;
Trypanosomatidae|Rep: PRP8 protein homologue, putative -
Leishmania infantum
Length = 2427
Score = 95.9 bits (228), Expect = 9e-19
Identities = 45/87 (51%), Positives = 60/87 (68%)
Frame = +2
Query: 254 LMKHDVNLGRAVFWDIKNRLPRSVTTIQWENSFVSVYSKDNPNLLFNMAGFECRILPKCR 433
L DVNL RAV W+ ++RLP S+ TI NSFVSVYSKDNPNLLF MAGF R+LP R
Sbjct: 1215 LFLSDVNLARAVLWEFRSRLPPSMATINDANSFVSVYSKDNPNLLFEMAGFSVRLLPVVR 1274
Query: 434 SQHEELSHRDGVWNLQNEVTKERTANA 514
++ ++ + +W+L+N TK+ + A
Sbjct: 1275 TE-ADILESESMWSLRNHTTKDISCRA 1300
Score = 74.5 bits (175), Expect = 2e-12
Identities = 38/90 (42%), Positives = 55/90 (61%), Gaps = 6/90 (6%)
Frame = +3
Query: 3 ASEMAGPPQLPNDFLSY-QDRQTETAHPIRLYCRYIDRIHIFFRFSAEEARDLIQRYLTE 179
+ E+AG PN F+S+ +D +HPIR YCRY + ++I R++ EA ++ +RYL E
Sbjct: 1125 SKEIAGTALKPNPFMSFFRDPYLAASHPIRAYCRYKNEVYIVVRYTKTEADEVRRRYLEE 1184
Query: 180 -HPDPN----NENIVGYNNKKCWPRDARMR 254
DP N ++ G+ N K WPRDARMR
Sbjct: 1185 TKSDPEMRAINASVYGFKNAKEWPRDARMR 1214
Score = 64.1 bits (149), Expect = 3e-09
Identities = 26/77 (33%), Positives = 48/77 (62%)
Frame = +1
Query: 508 KCYLRVDDESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLD 687
+ +L+V E + N+ R+ +M GS+TF I KWN + ++ Y+REA++ T+EL
Sbjct: 1299 RAFLQVTKEHINMIRNKARRTVMMVGSSTFHSIAAKWNALITEIVPYYREAILGTEELQA 1358
Query: 688 LLVKCENKIQTRIKIGL 738
+L + E+++Q R+ + L
Sbjct: 1359 ILARAEHRMQARVMMAL 1375
>UniRef50_Q9AW36 Cluster: Splicing factor Prp8; n=1; Guillardia
theta|Rep: Splicing factor Prp8 - Guillardia theta
(Cryptomonas phi)
Length = 2057
Score = 57.6 bits (133), Expect = 3e-07
Identities = 26/68 (38%), Positives = 42/68 (61%)
Frame = +1
Query: 535 SLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKI 714
S+ F +V+ L+ SGS++F+K++ KWN L+G +FR+A+V++Q L K E +I
Sbjct: 1061 SIKLFQLKVKNFLLTSGSSSFSKLIKKWNGLLLGYFCFFRKALVSSQNFTTRLKKYEKEI 1120
Query: 715 QTRIKIGL 738
IK L
Sbjct: 1121 IANIKASL 1128
>UniRef50_Q7QTZ9 Cluster: GLP_169_4270_455; n=1; Giardia lamblia
ATCC 50803|Rep: GLP_169_4270_455 - Giardia lamblia ATCC
50803
Length = 1271
Score = 55.2 bits (127), Expect = 2e-06
Identities = 24/67 (35%), Positives = 42/67 (62%)
Frame = +1
Query: 538 LARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQ 717
+A F Q++ + ST+F+KI+ KWN+ L+ + Y+REA++ + L +L+ E K+
Sbjct: 28 IANFIYHANQLISSGVSTSFSKIIAKWNSLLLNCVIYYREALLQSPRFLRILMAYEEKVC 87
Query: 718 TRIKIGL 738
+IK GL
Sbjct: 88 NKIKQGL 94
>UniRef50_Q8I5E6 Cluster: Putative uncharacterized protein; n=6;
Plasmodium|Rep: Putative uncharacterized protein -
Plasmodium falciparum (isolate 3D7)
Length = 1979
Score = 36.3 bits (80), Expect = 0.78
Identities = 21/72 (29%), Positives = 35/72 (48%)
Frame = +3
Query: 6 SEMAGPPQLPNDFLSYQDRQTETAHPIRLYCRYIDRIHIFFRFSAEEARDLIQRYLTEHP 185
S + G +L N +Y+D + P+ L C + D IH+FF++ Y + +
Sbjct: 1474 SLVLGICELENYISAYKDAFVDKDKPLLLVCGWPDNIHMFFKYLKRNV------YNSSYR 1527
Query: 186 DPNNENIVGYNN 221
+ NN NI Y+N
Sbjct: 1528 NKNNSNIHTYDN 1539
>UniRef50_UPI00006CE62B Cluster: hypothetical protein TTHERM_00706430;
n=1; Tetrahymena thermophila SB210|Rep: hypothetical
protein TTHERM_00706430 - Tetrahymena thermophila SB210
Length = 2189
Score = 33.9 bits (74), Expect = 4.2
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -1
Query: 294 QNTARPRLTSCFINAYEHPLASTFCYCNQQCSHCWDQD 181
Q + LT C N Y+ + +T C CNQ C C+ D
Sbjct: 1888 QQSLNQCLTQCNSNQYKDTINNTCCSCNQTCLTCFGPD 1925
>UniRef50_A4SB03 Cluster: Predicted protein; n=1; Ostreococcus
lucimarinus CCE9901|Rep: Predicted protein - Ostreococcus
lucimarinus CCE9901
Length = 3060
Score = 33.9 bits (74), Expect = 4.2
Identities = 20/58 (34%), Positives = 34/58 (58%), Gaps = 1/58 (1%)
Frame = +1
Query: 526 DDESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLM-TYFREAVVNTQELLDLLV 696
D S+A F++ M SG+T+F + ++ WNTAL+ M F++AV Q++ +V
Sbjct: 896 DTSSVANFNS------MFSGATSFNQDISSWNTALVTDMDNMFKDAVAFNQDISSWVV 947
>UniRef50_O17517 Cluster: Putative uncharacterized protein; n=1;
Caenorhabditis elegans|Rep: Putative uncharacterized
protein - Caenorhabditis elegans
Length = 1277
Score = 33.9 bits (74), Expect = 4.2
Identities = 23/76 (30%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
Frame = +1
Query: 520 RVDDESLARFHNR---VRQILMASG--STTFTKIVNKWNTALIGLMTYFREAVVNTQELL 684
R++D+ LA R V +++A+ S K+ NK T GL RE N Q
Sbjct: 332 RMEDKKLANTIARNLYVDNLIIATDDDSEAMFKLYNKVKTVFNGLSMNIREFQSNDQSFT 391
Query: 685 DLLVKCENKIQTRIKI 732
DLL +C+ ++ +K+
Sbjct: 392 DLLDECDKTSESEVKV 407
>UniRef50_Q5QCH6 Cluster: Maturase; n=2; Rhizanthes|Rep: Maturase -
Rhizanthes infanticida
Length = 485
Score = 33.1 bits (72), Expect = 7.3
Identities = 21/69 (30%), Positives = 32/69 (46%)
Frame = +1
Query: 532 ESLARFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENK 711
E L R+ Q L SGS TKI + +G T RE +T+ + L + E +
Sbjct: 269 ELLYEIKKRIAQFLQGSGSAGSTKIAARSTVEFLG--TVLREVPTSTRTPIQLFRELEKR 326
Query: 712 IQTRIKIGL 738
I+ + +I L
Sbjct: 327 IRVKHRIHL 335
>UniRef50_A3DFA7 Cluster: Spore coat assembly protein-like protein;
n=1; Clostridium thermocellum ATCC 27405|Rep: Spore coat
assembly protein-like protein - Clostridium thermocellum
(strain ATCC 27405 / DSM 1237)
Length = 709
Score = 32.7 bits (71), Expect = 9.7
Identities = 16/37 (43%), Positives = 23/37 (62%)
Frame = +2
Query: 401 GFECRILPKCRSQHEELSHRDGVWNLQNEVTKERTAN 511
G E +P R EE+S+RDG +QN+ TKE+ +N
Sbjct: 272 GIEREQMPD-RPGREEISNRDGTKQIQNQETKEQNSN 307
>UniRef50_A2DU71 Cluster: Putative uncharacterized protein; n=1;
Trichomonas vaginalis G3|Rep: Putative uncharacterized
protein - Trichomonas vaginalis G3
Length = 151
Score = 32.7 bits (71), Expect = 9.7
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -1
Query: 417 ILHSNPAILNNKLGLSLE*TDTKLFSHCIVVTERGRRFFM 298
+L N IL N G L DT+LFS V+ E+ +R+F+
Sbjct: 72 VLPLNGQILVNTNGFILNDNDTELFSQSFVLVEKSKRYFI 111
>UniRef50_O67554 Cluster: Uncharacterized kamA family protein
aq_1632; n=1; Aquifex aeolicus|Rep: Uncharacterized kamA
family protein aq_1632 - Aquifex aeolicus
Length = 374
Score = 32.7 bits (71), Expect = 9.7
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = +3
Query: 90 LYCRYIDRIHIFFRFSAEEARDLIQ--RYLTEHPDPNNENIVG 212
+YCR+ R +F + E ARD+ + Y+ HP+ NN + G
Sbjct: 102 IYCRFCFRKRLFMNDNDEVARDVSEGLEYIRNHPEINNVLLTG 144
>UniRef50_Q9PJP6 Cluster: ADP,ATP carrier protein 2; n=8;
Chlamydiaceae|Rep: ADP,ATP carrier protein 2 - Chlamydia
muridarum
Length = 543
Score = 32.7 bits (71), Expect = 9.7
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 470 WNLQNEVTKERTANATYALMTNPWLVSTIVSDK-SLWLPDQRPLPKS 607
W + N +T R A YAL+ VS+IV+ + SLWL +P S
Sbjct: 168 WGIANHITSVREAGRFYALINVGLNVSSIVAGEISLWLGKHTLIPSS 214
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 764,013,684
Number of Sequences: 1657284
Number of extensions: 15583186
Number of successful extensions: 38392
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 37073
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38387
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 60088620670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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