Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20796
(588 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g35470.2 68414.m04401 SPla/RYanodine receptor (SPRY) domain-c... 83 1e-16
At1g35470.1 68414.m04400 SPla/RYanodine receptor (SPRY) domain-c... 83 1e-16
At4g09340.1 68417.m01543 SPla/RYanodine receptor (SPRY) domain-c... 69 2e-12
At4g09310.1 68417.m01539 SPla/RYanodine receptor (SPRY) domain-c... 59 2e-09
At4g09250.1 68417.m01532 SPla/RYanodine receptor (SPRY) domain-c... 59 2e-09
At4g09200.1 68417.m01522 SPla/RYanodine receptor (SPRY) domain-c... 59 2e-09
At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger) fa... 47 8e-06
At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY) domain-c... 39 0.002
At4g06536.1 68417.m00948 hypothetical protein 31 0.43
At1g76440.2 68414.m08888 expressed protein 29 2.3
At1g76440.1 68414.m08887 expressed protein 29 2.3
At2g28320.1 68415.m03442 pleckstrin homology (PH) domain-contain... 28 4.0
At2g28110.1 68415.m03415 exostosin family protein contains 1 tra... 28 4.0
At4g00940.1 68417.m00127 Dof-type zinc finger domain-containing ... 28 5.3
At3g49110.1 68416.m05364 peroxidase 33 (PER33) (P33) (PRXCA) / n... 27 9.3
At2g01940.1 68415.m00129 zinc finger (C2H2 type) family protein ... 27 9.3
>At1g35470.2 68414.m04401 SPla/RYanodine receptor (SPRY)
domain-containing protein similar to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 467
Score = 83.4 bits (197), Expect = 1e-16
Identities = 34/81 (41%), Positives = 46/81 (56%)
Frame = +1
Query: 16 DAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSYGYHGD 195
D V+A P P C YYFE+ + G G + IG + M R PGW+ +S GYHGD
Sbjct: 83 DVGVVQANKPAPIKCLTYYFEIFVKDSGIKGQIAIGFTKESFKMRRQPGWEVNSCGYHGD 142
Query: 196 DGHSFCSSGTGQPYGPTFTTE 258
DG+ + G G+P+GP FT +
Sbjct: 143 DGYLYRGQGKGEPFGPKFTKD 163
Score = 57.6 bits (133), Expect = 6e-09
Identities = 27/81 (33%), Positives = 46/81 (56%)
Frame = +3
Query: 258 DVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQPFVFDI 437
D +G G+N F+TKNG +G + + +L+PTV + + E V NFG++ F FDI
Sbjct: 164 DAVGGGINYASQEFFFTKNGTIVGKIPKDIRGHLFPTVAVHSQNEEVLVNFGKKKFAFDI 223
Query: 438 EDMLKELRARTRVAIDEFPLP 500
+ R + ++AI++ +P
Sbjct: 224 KGYEASERNKQQLAIEKISIP 244
>At1g35470.1 68414.m04400 SPla/RYanodine receptor (SPRY)
domain-containing protein similar to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 465
Score = 83.4 bits (197), Expect = 1e-16
Identities = 34/81 (41%), Positives = 46/81 (56%)
Frame = +1
Query: 16 DAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSYGYHGD 195
D V+A P P C YYFE+ + G G + IG + M R PGW+ +S GYHGD
Sbjct: 83 DVGVVQANKPAPIKCLTYYFEIFVKDSGIKGQIAIGFTKESFKMRRQPGWEVNSCGYHGD 142
Query: 196 DGHSFCSSGTGQPYGPTFTTE 258
DG+ + G G+P+GP FT +
Sbjct: 143 DGYLYRGQGKGEPFGPKFTKD 163
Score = 57.6 bits (133), Expect = 6e-09
Identities = 27/81 (33%), Positives = 46/81 (56%)
Frame = +3
Query: 258 DVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQPFVFDI 437
D +G G+N F+TKNG +G + + +L+PTV + + E V NFG++ F FDI
Sbjct: 164 DAVGGGINYASQEFFFTKNGTIVGKIPKDIRGHLFPTVAVHSQNEEVLVNFGKKKFAFDI 223
Query: 438 EDMLKELRARTRVAIDEFPLP 500
+ R + ++AI++ +P
Sbjct: 224 KGYEASERNKQQLAIEKISIP 244
>At4g09340.1 68417.m01543 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 447
Score = 69.3 bits (162), Expect = 2e-12
Identities = 29/80 (36%), Positives = 44/80 (55%)
Frame = +1
Query: 16 DAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSYGYHGD 195
D V+A P P YYFE+ + G G + IG + + + R PGW+ ++ GYHG+
Sbjct: 62 DVGVVQANKPAPCKRFAYYFEIYVKDAGVKGRVSIGFTTNSFRIARHPGWELNTCGYHGE 121
Query: 196 DGHSFCSSGTGQPYGPTFTT 255
DG + G+ +GPT+TT
Sbjct: 122 DGLIYLGKRQGEAFGPTYTT 141
Score = 59.3 bits (137), Expect = 2e-09
Identities = 27/82 (32%), Positives = 44/82 (53%)
Frame = +3
Query: 255 GDVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQPFVFD 434
GD +G G+N F+T NG +G + + L+PTV + + E V NFGQ+ F FD
Sbjct: 142 GDTVGGGINYDSQEFFFTVNGTLVGTVSKYIKGPLFPTVAVHSQNEEVTVNFGQEKFAFD 201
Query: 435 IEDMLKELRARTRVAIDEFPLP 500
+ R + ++AI++ +P
Sbjct: 202 FKGYESSERNKQQIAIEKISIP 223
>At4g09310.1 68417.m01539 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 397
Score = 59.3 bits (137), Expect = 2e-09
Identities = 30/82 (36%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = +3
Query: 255 GDVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQ-PFVF 431
GD++GCG++ V F+TKNG +G R +YPT+ L + E V NFG Q F F
Sbjct: 123 GDLVGCGIDSVSQEFFFTKNGTIVGTIPRQFRRPVYPTIVLHSQNEAVTVNFGGQIAFSF 182
Query: 432 DIEDMLKELRARTRVAIDEFPL 497
D E + LR + I++ +
Sbjct: 183 DFEHFEESLRVKKEREIEKISM 204
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/76 (31%), Positives = 34/76 (44%)
Frame = +1
Query: 1 GKTHRDAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSY 180
G H D ++ +P P C YYFEV I++ G G + IG S + G+ S
Sbjct: 38 GPDH-DGVVLQTENPAPTNCLAYYFEVHIMNAGEKGEIAIGFSKEHIYSE---GYTVRSC 93
Query: 181 GYHGDDGHSFCSSGTG 228
Y G+ G +G G
Sbjct: 94 AYIGNSGLICSQNGDG 109
>At4g09250.1 68417.m01532 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 427
Score = 59.3 bits (137), Expect = 2e-09
Identities = 30/82 (36%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = +3
Query: 255 GDVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQ-PFVF 431
GD++GCG++ V F+TKNG +G R +YPT+ L + E V NFG Q F F
Sbjct: 201 GDLVGCGIDSVSQEFFFTKNGTIVGTIPRQFRRPVYPTIVLHSQNEAVTVNFGGQIAFSF 260
Query: 432 DIEDMLKELRARTRVAIDEFPL 497
D E + LR + I++ +
Sbjct: 261 DFEHFEESLRVKKEREIEKISM 282
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/76 (31%), Positives = 34/76 (44%)
Frame = +1
Query: 1 GKTHRDAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSY 180
G H D ++ +P P C YYFEV I++ G G + IG S + G+ S
Sbjct: 116 GPDH-DGVVLQTENPAPTNCLAYYFEVHIMNAGEKGEIAIGFSKEHIYSE---GYTVRSC 171
Query: 181 GYHGDDGHSFCSSGTG 228
Y G+ G +G G
Sbjct: 172 AYIGNSGLICSQNGDG 187
>At4g09200.1 68417.m01522 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to RanBPM [Homo
sapiens] GI:15080674; contains Pfam profile PF00622:
SPRY domain
Length = 397
Score = 59.3 bits (137), Expect = 2e-09
Identities = 30/82 (36%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = +3
Query: 255 GDVIGCGVNLVDNTCFYTKNGHHLGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQ-PFVF 431
GD++GCG++ V F+TKNG +G R +YPT+ L + E V NFG Q F F
Sbjct: 123 GDLVGCGIDSVSQEFFFTKNGTIVGTIPRQFRRPVYPTIVLHSQNEAVTVNFGGQIAFSF 182
Query: 432 DIEDMLKELRARTRVAIDEFPL 497
D E + LR + I++ +
Sbjct: 183 DFEHFEESLRVKKEREIEKISM 204
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/76 (31%), Positives = 34/76 (44%)
Frame = +1
Query: 1 GKTHRDAASVRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSY 180
G H D ++ +P P C YYFEV I++ G G + IG S + G+ S
Sbjct: 38 GPDH-DGVVLQTENPAPTNCLAYYFEVHIMNAGEKGEIAIGFSKEHIYSE---GYTVRSC 93
Query: 181 GYHGDDGHSFCSSGTG 228
Y G+ G +G G
Sbjct: 94 AYIGNSGLICSQNGDG 109
>At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 1280
Score = 47.2 bits (107), Expect = 8e-06
Identities = 29/78 (37%), Positives = 41/78 (52%), Gaps = 5/78 (6%)
Frame = +3
Query: 255 GDVIGCGVNLVDNTCFYTKNGHHLGIAFRG---LPPNL--YPTVGLQTPGEVVDTNFGQQ 419
GDVIGC ++L + ++ +NG LG AF G L P YP + L + GE + NFG
Sbjct: 209 GDVIGCCIDLNCDEIYFYRNGVSLGAAFTGIRKLGPGFGYYPAISL-SQGERCELNFGAY 267
Query: 420 PFVFDIEDMLKELRARTR 473
PF + ++ A TR
Sbjct: 268 PFKYPVDGFQPLQEAPTR 285
>At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY)
domain-containing protein low similarity to DEAD box
protein DDX1 [Gallus gallus] GI:16323037, ryanodine
receptor [Caenorhabditis elegans] GI:1871447; contains
Pfam profile PF00622: SPRY domain
Length = 509
Score = 39.1 bits (87), Expect = 0.002
Identities = 19/58 (32%), Positives = 33/58 (56%)
Frame = +1
Query: 28 VRATHPIPAACGLYYFEVRIVSKGRDGYMGIGLSAHGVNMNRLPGWDKHSYGYHGDDG 201
VRAT + G +YFE++++S G G+ +G S ++ G+D +S+G+ DG
Sbjct: 302 VRATRGVVE--GAWYFEIKVLSLGETGHTRLGWSTDKGDLQAPVGYDGNSFGFRDIDG 357
>At4g06536.1 68417.m00948 hypothetical protein
Length = 169
Score = 31.5 bits (68), Expect = 0.43
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = +3
Query: 252 DGDVIGCGVNLVDNTCFYTKNGH--H-LGIAFRGLPPNLYPTVGLQTPGEVVDTNFGQQP 422
+ V+GCG + ++T N H H + LYPT+ T V+ N GQ P
Sbjct: 18 ENKVVGCGYDPRKKKVYFTVNSHLVHVINCKADEFGTPLYPTLASNTEARVL-VNLGQSP 76
Query: 423 FVF 431
F +
Sbjct: 77 FYY 79
>At1g76440.2 68414.m08888 expressed protein
Length = 143
Score = 29.1 bits (62), Expect = 2.3
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -1
Query: 171 LVPPGQSVHVDSMCGQSDPHVPVPSFRYDSNLEVV 67
L PPG ++ GQ DP + P+FR D EVV
Sbjct: 98 LCPPGPFSITFNLPGQVDPRLFSPNFRSDGIFEVV 132
>At1g76440.1 68414.m08887 expressed protein
Length = 143
Score = 29.1 bits (62), Expect = 2.3
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -1
Query: 171 LVPPGQSVHVDSMCGQSDPHVPVPSFRYDSNLEVV 67
L PPG ++ GQ DP + P+FR D EVV
Sbjct: 98 LCPPGPFSITFNLPGQVDPRLFSPNFRSDGIFEVV 132
>At2g28320.1 68415.m03442 pleckstrin homology (PH) domain-containing
protein / lipid-binding START domain-containing protein
contains Pfam profiles PF01852: START domain, PF00169:
PH domain
Length = 737
Score = 28.3 bits (60), Expect = 4.0
Identities = 15/47 (31%), Positives = 21/47 (44%)
Frame = +1
Query: 166 DKHSYGYHGDDGHSFCSSGTGQPYGPTFTTETSSDVESTWSTTRAST 306
D H H+ C+ YG T T+ S D+ +W+TT ST
Sbjct: 484 DLHERAKEESSPHATCNPPCC--YGTTLPTDPSCDLPCSWTTTDPST 528
>At2g28110.1 68415.m03415 exostosin family protein contains 1
transmembrane domain; similar to
pectin-glucuronyltransferase (GI:23821292) [Nicotiana
plumbaginifolia]; similar to NpGUT1 homolog
(GI:23821294) [Arabidopsis thaliana]; contains Pfam
profile PF03016: Exostosin family
Length = 448
Score = 28.3 bits (60), Expect = 4.0
Identities = 22/68 (32%), Positives = 25/68 (36%)
Frame = -1
Query: 549 YVDTIVCNTWCHCPARRAAGTHRWRPWSERAAPSACPRCRKRTAADRSSCPRPRRASEAR 370
Y I + +C CP W PWS R S C AD P P S R
Sbjct: 321 YQSEIARSVFCLCPLG-------WAPWSPRLVESVALGCVPVIIADGIRLPFP---STVR 370
Query: 369 LSDINLAV 346
DI+L V
Sbjct: 371 WPDISLTV 378
>At4g00940.1 68417.m00127 Dof-type zinc finger domain-containing
protein similar to DNA-binding protein DAG1/BBFa
GI:4581965 [Arabidopsis thaliana]
Length = 294
Score = 27.9 bits (59), Expect = 5.3
Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Frame = +1
Query: 166 DKHSYGYHGDDGHSFCSSGTGQPYGPTFTTE---TSSDVESTWSTT 294
D H Y +H H +S Y TT+ T++ ++ST +TT
Sbjct: 2 DHHQYHHHDQYQHQMMTSTNNNSYNTIVTTQPPPTTTTMDSTTATT 47
>At3g49110.1 68416.m05364 peroxidase 33 (PER33) (P33) (PRXCA) /
neutral peroxidase C (PERC) identical to SP|P24101
Peroxidase 33 precursor (EC 1.11.1.7) (Atperox P33)
(ATPCa) (Neutral peroxidase C) (PERC) {Arabidopsis
thaliana}
Length = 354
Score = 27.1 bits (57), Expect = 9.3
Identities = 18/42 (42%), Positives = 21/42 (50%), Gaps = 3/42 (7%)
Frame = +3
Query: 234 LRTHVHDGDVIGCGVN-LVDNTCFYTKNGHHLGIA--FRGLP 350
LR H HD V GC + L+DNT + LG A RG P
Sbjct: 68 LRLHFHDCFVNGCDASILLDNTTSFRTEKDALGNANSARGFP 109
>At2g01940.1 68415.m00129 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 439
Score = 27.1 bits (57), Expect = 9.3
Identities = 18/70 (25%), Positives = 32/70 (45%)
Frame = -2
Query: 578 PCCSTRGAPGTSTPSCATPGATVLLVGQRELIDGDPGPSAQLLQHVLDVENERLLTEVRV 399
P CS+R A STPS T + V + ++G P ++ +L + L E+++
Sbjct: 213 PACSSRTASTVSTPSSETNYGGTVAVTTPQPLEGRP-IHQRISSSILTNSSNNLNLELQL 271
Query: 398 HDLAGRLKPD 369
L+ P+
Sbjct: 272 LPLSSNQNPN 281
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,251,611
Number of Sequences: 28952
Number of extensions: 292597
Number of successful extensions: 902
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 900
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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