Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20773
(815 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 32 0.11
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 31 0.20
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 30 0.45
SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr 1|||M... 29 1.0
SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalyt... 28 1.4
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 28 1.8
SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin Nup132|Schizosa... 27 3.2
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 5.6
SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomy... 26 7.4
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 26 7.4
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 31.9 bits (69), Expect = 0.11
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +2
Query: 578 VAFNLKNYQKPKEQLRMQHRYIDLRFPVMQNNL-RTRSQMLHKMRRFLVENYGFIEVETP 754
+ L NY+K R + R++D+ L R +++ +R+F E GF+EVETP
Sbjct: 176 IPVKLTNYEK-----RFEKRFVDMMSNTKSLELLEKRYRIIESIRKFFSER-GFLEVETP 229
Query: 755 TL 760
L
Sbjct: 230 IL 231
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 31.1 bits (67), Expect = 0.20
Identities = 21/64 (32%), Positives = 31/64 (48%), Gaps = 8/64 (12%)
Frame = +1
Query: 328 RVVICGWVQYSRLSK---FLLLRDAYGLAQCIVGCD-----NIDLSSLQLESVVQIEGMV 483
RV + GWV R K F++LRD G QC++ + D +L ES V + G++
Sbjct: 143 RVRVNGWVHRMRTQKGIIFIILRDGTGFLQCVLSGKVYDRASYDFINLGPESTVCLYGVI 202
Query: 484 SIRP 495
P
Sbjct: 203 KELP 206
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 29.9 bits (64), Expect = 0.45
Identities = 28/84 (33%), Positives = 39/84 (46%), Gaps = 13/84 (15%)
Frame = +1
Query: 283 RTHT-CGELRPKNVGERVVICGWVQYSRLSK----FLLLRDAYGLAQC--IVGCDNID-- 435
RT+T ++ KN G+ V++ V SRL F LR Y Q +V D I
Sbjct: 90 RTYTQISDISAKNDGQTVLLRARVYTSRLQGNKMCFFSLRQKYDTIQALAVVNKDTISKQ 149
Query: 436 ----LSSLQLESVVQIEGMVSIRP 495
S+ LES+V +EG+V P
Sbjct: 150 MVKWCGSISLESIVLVEGIVKKSP 173
>SPAC20G8.02 |||phospholipase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 757
Score = 28.7 bits (61), Expect = 1.0
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 317 MSVKESSYAAGCSIHVCPNFYCFETPTD 400
+S E S+ +G SI C NFY PTD
Sbjct: 585 LSYSEQSFDSGVSILSCQNFYNIFHPTD 612
>SPBC106.10 |pka1|tpk, git6|cAMP-dependent protein kinase catalytic
subunit Pka1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/36 (41%), Positives = 21/36 (58%)
Frame = -1
Query: 755 LESQLR*THNFPLEISASYAASVI*FLNYFASREIL 648
L S LR H FP +++ YAA VI L+Y +I+
Sbjct: 286 LFSLLRKCHRFPEKVAKFYAAEVILALDYLHHNQIV 321
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 27.9 bits (59), Expect = 1.8
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +1
Query: 760 FCRTPGGAREFVVPTR 807
F TP GAREFVVP+R
Sbjct: 187 FKSTPEGAREFVVPSR 202
>SPAC1805.04 |nup132|Nup133b, Nup133b|nucleoporin
Nup132|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1162
Score = 27.1 bits (57), Expect = 3.2
Identities = 16/56 (28%), Positives = 30/56 (53%)
Frame = +2
Query: 617 QLRMQHRYIDLRFPVMQNNLRTRSQMLHKMRRFLVENYGFIEVETPTLFAGHQVVL 784
QLR D+ + +++ T S +L KM+ F + I +++P LF+ +Q +L
Sbjct: 200 QLRDPAGQPDVSVQLFASDISTFSTILQKMKIFNYPSIHIIALKSPPLFSPYQHLL 255
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2
Query: 44 TEIVTMLGHKINSNKIKNVCTVFRSTNILKHLCNNN 151
TE++ LG N ++N VF T H NN+
Sbjct: 496 TELLDQLGIPKNQRSLQNATNVFEPTEFTFHSGNNS 531
>SPBC16G5.18 |erg24||C-14 sterol reductase Erg24|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 424
Score = 25.8 bits (54), Expect = 7.4
Identities = 14/58 (24%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -2
Query: 580 YFFCGFKTFKLIMVTSISPVAISGSLCLRALWKPCLQFVLQILVVSWI-NQYCHNQQY 410
Y GF + LI+ + + + G+ C+ +W LQ + V S + +C+ Q +
Sbjct: 102 YKINGFNSACLILGVVCTSIYLLGASCMEFIWDNFLQLMFAAYVFSVVLCTFCYVQSF 159
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 7.4
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +1
Query: 472 EGMVSIRPGDTVTLKWLP 525
EG++++RPGD V L + P
Sbjct: 80 EGVINVRPGDHVILLYTP 97
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,305,177
Number of Sequences: 5004
Number of extensions: 66516
Number of successful extensions: 155
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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