Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20773
(815 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) f... 55 5e-08
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 40 0.003
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 37 0.014
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 36 0.032
At2g04680.1 68415.m00478 DC1 domain-containing protein contains ... 29 3.7
At3g46800.1 68416.m05080 DC1 domain-containing protein contains ... 29 4.9
At3g27500.1 68416.m03438 DC1 domain-containing protein contains ... 29 4.9
At5g13890.3 68418.m01625 expressed protein contains Pfam profile... 28 6.4
At5g13890.2 68418.m01624 expressed protein contains Pfam profile... 28 6.4
At5g13890.1 68418.m01623 expressed protein contains Pfam profile... 28 6.4
At5g63040.2 68418.m07909 expressed protein similar to unknown pr... 28 8.5
At5g63040.1 68418.m07908 expressed protein similar to unknown pr... 28 8.5
>At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N)
family protein similar to SP|P36419 Aspartyl-tRNA
synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) {Thermus thermophilus}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 664
Score = 55.2 bits (127), Expect = 5e-08
Identities = 34/86 (39%), Positives = 46/86 (53%), Gaps = 8/86 (9%)
Frame = +1
Query: 283 RTHTCGELRPKNVGERVVICGWVQYSRLS---KFLLLRDAYGLAQCIVGCDNID-----L 438
RT CGEL +VG+RV +CGWV R+ FL LRD G+ Q D +
Sbjct: 75 RTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGIVQVRTLPDEFPEAHGLI 134
Query: 439 SSLQLESVVQIEGMVSIRPGDTVTLK 516
+ ++LE VV +EG V RP ++V K
Sbjct: 135 NDMRLEYVVLVEGTVRSRPNESVNKK 160
Score = 51.6 bits (118), Expect = 6e-07
Identities = 22/50 (44%), Positives = 36/50 (72%)
Frame = +2
Query: 611 KEQLRMQHRYIDLRFPVMQNNLRTRSQMLHKMRRFLVENYGFIEVETPTL 760
KE++R++ R +DLR M+NN+ R ++ +RR+L + +GFIE+ETP L
Sbjct: 198 KEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRHGFIEIETPIL 247
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 39.5 bits (88), Expect = 0.003
Identities = 22/72 (30%), Positives = 37/72 (51%)
Frame = +2
Query: 554 KSFKSAEKVAFNLKNYQKPKEQLRMQHRYIDLRFPVMQNNLRTRSQMLHKMRRFLVENYG 733
+S EK + K + + R+ +R +D+R P Q R + Q+ R +L ++ G
Sbjct: 187 RSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYL-QSKG 245
Query: 734 FIEVETPTLFAG 769
F+E+ TP L AG
Sbjct: 246 FLEIHTPKLIAG 257
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 37.1 bits (82), Expect = 0.014
Identities = 20/59 (33%), Positives = 37/59 (62%), Gaps = 1/59 (1%)
Frame = +2
Query: 587 NLKNYQKPKEQLRMQHRYIDLRFPV-MQNNLRTRSQMLHKMRRFLVENYGFIEVETPTL 760
N + Y ++ R + R++D+ V ++ RTR++++ +RRFL +N F+EVETP +
Sbjct: 250 NPEAYVLKDQESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFL-DNKNFLEVETPMM 307
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 35.9 bits (79), Expect = 0.032
Identities = 17/47 (36%), Positives = 33/47 (70%), Gaps = 1/47 (2%)
Frame = +2
Query: 623 RMQHRYIDL-RFPVMQNNLRTRSQMLHKMRRFLVENYGFIEVETPTL 760
R + RY+D+ P + + R R++++ ++R+ VE++G++EVETP L
Sbjct: 234 RYRQRYVDMIANPEVADVFRRRAKIVSEIRK-TVESFGYLEVETPVL 279
>At2g04680.1 68415.m00478 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 657
Score = 29.1 bits (62), Expect = 3.7
Identities = 15/54 (27%), Positives = 24/54 (44%)
Frame = -3
Query: 813 FMTSGDNKFSSTTWCPAKSVGVSTSINP*FSTRNLRILCSICDLVLKLFCITGN 652
F++ GD S WC A T +NP + C+ C +L + C+ G+
Sbjct: 549 FLSFGDKNVSGDYWCEA----CETKVNP----KEWFYTCNDCGTILHISCVVGD 594
>At3g46800.1 68416.m05080 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 682
Score = 28.7 bits (61), Expect = 4.9
Identities = 12/54 (22%), Positives = 26/54 (48%)
Frame = -3
Query: 813 FMTSGDNKFSSTTWCPAKSVGVSTSINP*FSTRNLRILCSICDLVLKLFCITGN 652
+++ G+NK WC T ++P + C++C ++L + C+ G+
Sbjct: 569 YLSYGENKEDGQLWCEV----CETKVDP----KEWLYTCNLCGIILHISCVVGD 614
>At3g27500.1 68416.m03438 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 609
Score = 28.7 bits (61), Expect = 4.9
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 723 STRNLRILCSICDLVLKLFCITGNL 649
+T+ L CS+C L L +FCI +L
Sbjct: 91 NTKRLLFHCSVCQLNLDIFCIADHL 115
>At5g13890.3 68418.m01625 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 310
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -2
Query: 532 ISPVAISGSLCLRALWKPCLQFVLQILVVSWINQYCH 422
I+ A++ ++CL+ LW+ LQ L + V +I + CH
Sbjct: 191 IADAALAAAICLQGLWQ--LQTGLSLYVDGFIPEGCH 225
>At5g13890.2 68418.m01624 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 310
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -2
Query: 532 ISPVAISGSLCLRALWKPCLQFVLQILVVSWINQYCH 422
I+ A++ ++CL+ LW+ LQ L + V +I + CH
Sbjct: 191 IADAALAAAICLQGLWQ--LQTGLSLYVDGFIPEGCH 225
>At5g13890.1 68418.m01623 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 310
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = -2
Query: 532 ISPVAISGSLCLRALWKPCLQFVLQILVVSWINQYCH 422
I+ A++ ++CL+ LW+ LQ L + V +I + CH
Sbjct: 191 IADAALAAAICLQGLWQ--LQTGLSLYVDGFIPEGCH 225
>At5g63040.2 68418.m07909 expressed protein similar to unknown
protein (emb|CAB81845.1)
Length = 366
Score = 27.9 bits (59), Expect = 8.5
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = -2
Query: 637 SVLHSQLFFWFLIVF*VECYFFCGFKTFKLIM 542
+V L FLI+F E F+CG F LI+
Sbjct: 176 AVFEDSLLTDFLILFFTEALFYCGVAAFLLII 207
>At5g63040.1 68418.m07908 expressed protein similar to unknown
protein (emb|CAB81845.1)
Length = 366
Score = 27.9 bits (59), Expect = 8.5
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = -2
Query: 637 SVLHSQLFFWFLIVF*VECYFFCGFKTFKLIM 542
+V L FLI+F E F+CG F LI+
Sbjct: 176 AVFEDSLLTDFLILFFTEALFYCGVAAFLLII 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,006,823
Number of Sequences: 28952
Number of extensions: 335789
Number of successful extensions: 810
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 809
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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