Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20772
         (470 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   109   2e-25
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   105   3e-24
SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s...    28   0.62 
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi...    27   1.4  
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch...    27   1.4  
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom...    26   2.5  
SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual           26   3.3  
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi...    26   3.3  
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M...    26   3.3  
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa...    25   4.4  
SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T...    25   4.4  
SPBC18H10.05 |||WD repeat protein Wdr44 family, WD repeat protei...    25   7.7  
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    25   7.7  

>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  109 bits (263), Expect = 2e-25
 Identities = 51/75 (68%), Positives = 62/75 (82%)
 Frame = +3

Query: 246 F*GSVEVQDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGG 425
           F GSVE++DG LV++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GG
Sbjct: 56  FDGSVEIKDGKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGG 115

Query: 426 AKKVIISAPSADAPM 470
           AK+VIISAPS DAPM
Sbjct: 116 AKRVIISAPSKDAPM 130



 Score = 90.6 bits (215), Expect = 1e-19
 Identities = 42/65 (64%), Positives = 51/65 (78%), Gaps = 1/65 (1%)
 Frame = +1

Query: 97  KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGVLRFRM 273
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF G +  + 
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 274 DSLLL 288
             L++
Sbjct: 65  GKLVI 69


>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  105 bits (252), Expect = 3e-24
 Identities = 50/75 (66%), Positives = 60/75 (80%)
 Frame = +3

Query: 246 F*GSVEVQDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGG 425
           F GSVE + G LV++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GG
Sbjct: 56  FEGSVETKGGKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGG 115

Query: 426 AKKVIISAPSADAPM 470
           AK+VIISAPS DAPM
Sbjct: 116 AKRVIISAPSKDAPM 130



 Score = 91.1 bits (216), Expect = 8e-20
 Identities = 42/65 (64%), Positives = 52/65 (80%), Gaps = 1/65 (1%)
 Frame = +1

Query: 97  KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGVLRFRM 273
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+G +  + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 274 DSLLL 288
             L++
Sbjct: 65  GKLVI 69


>SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3
           subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 821

 Score = 28.3 bits (60), Expect = 0.62
 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
 Frame = -3

Query: 258 HSLKTAMGGIILEKINHIVKTDERVIY-SDHLSS 160
           HSL+T +     E INH+V   E V + +DHL +
Sbjct: 476 HSLETVVDKAYTESINHLVYLMEEVFHLTDHLKA 509


>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
           Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 27.1 bits (57), Expect = 1.4
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNKFSITCREHVKN 101
           LHLVL +RGGG+   K + T  + +K+
Sbjct: 67  LHLVLRLRGGGKKRKKKTYTTPKKIKH 93


>SPAC589.10c |||ribomal-ubiquitin fusion protein
           Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 150

 Score = 27.1 bits (57), Expect = 1.4
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNKFSITCREHVKN 101
           LHLVL +RGGG+   K + T  + +K+
Sbjct: 67  LHLVLRLRGGGKKRKKKTYTTPKKIKH 93


>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 26.2 bits (55), Expect = 2.5
 Identities = 16/62 (25%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
 Frame = -1

Query: 461 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPRNGLRVPF*E-NSNFVTIN 285
           + +G  +N  +S    +  C +C G     + ++ +P F  R  +R+P    N N  TI 
Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGF--RQQMRMPCNACNGNGRTIK 210

Query: 284 NK 279
           +K
Sbjct: 211 HK 212


>SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 604

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = -1

Query: 347 FSPRNGLRVPF*ENSNFVTINNKESILNLNT 255
           FSP     V   E+++  +++N +S+ NLN+
Sbjct: 67  FSPSKATTVSLKESASLTSLSNNKSVSNLNS 97


>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
           Usp107|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 9/22 (40%), Positives = 16/22 (72%)
 Frame = +2

Query: 53  SVIQQIFYNLPRTCQKLESMDL 118
           +++QQI +NL R CQ + + D+
Sbjct: 245 NILQQIRFNLERICQDIGNFDV 266


>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 382

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNK 68
           LHLVL +RGG Q+F K
Sbjct: 67  LHLVLRLRGGMQIFVK 82



 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNK 68
           LHLVL +RGG Q+F K
Sbjct: 143 LHLVLRLRGGMQIFVK 158



 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNK 68
           LHLVL +RGG Q+F K
Sbjct: 219 LHLVLRLRGGMQIFVK 234



 Score = 25.8 bits (54), Expect = 3.3
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +3

Query: 21  LHLVLNVRGGGQLFNK 68
           LHLVL +RGG Q+F K
Sbjct: 295 LHLVLRLRGGMQIFVK 310


>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
           Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 502

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 14/37 (37%), Positives = 19/37 (51%)
 Frame = +1

Query: 112 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 222
           GF RI    +R   EKG QVV   D  I  + + +L+
Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334


>SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 988

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 11/21 (52%), Positives = 12/21 (57%)
 Frame = +3

Query: 60  FNKFSITCREHVKNWNQWIWP 122
           FN  SI  R  V  W QWI+P
Sbjct: 244 FNLHSICDRNTVHMWLQWIFP 264


>SPBC18H10.05 |||WD repeat protein Wdr44 family, WD repeat
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 586

 Score = 24.6 bits (51), Expect = 7.7
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = -2

Query: 406 DALSVVVKTPVDSTTYSAPAFPHGMALGSLSEKTAIL 296
           D  S +V TP DSTTY+    P+   L    +  ++L
Sbjct: 84  DDNSSLVNTPRDSTTYAETNSPNTEKLAGRDDDRSLL 120


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 24.6 bits (51), Expect = 7.7
 Identities = 12/36 (33%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
 Frame = -1

Query: 386 KDTSRL-YNIFSPSFSPRNGLRVPF*ENSNFVTINN 282
           K TS+L  +  +P+F+P+    +P  +   FV +NN
Sbjct: 17  KQTSKLSMSAKAPTFTPKAAPFIPSFQRPGFVPVNN 52


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,074,205
Number of Sequences: 5004
Number of extensions: 43472
Number of successful extensions: 173
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 180421690
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)