Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20772
(470 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 104 3e-23
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 101 2e-22
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 96 1e-20
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 94 4e-20
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 70 9e-13
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 64 6e-11
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 63 8e-11
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 29 1.6
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 29 1.6
At4g01330.1 68417.m00173 protein kinase family protein contains ... 29 1.6
At5g24900.1 68418.m02948 cytochrome P450 family protein similar ... 29 2.1
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 29 2.1
At1g56010.2 68414.m06428 transcription activator NAC1 (NAC1) con... 29 2.1
At1g56010.1 68414.m06427 transcription activator NAC1 (NAC1) con... 29 2.1
At4g04710.1 68417.m00692 calcium-dependent protein kinase, putat... 28 3.7
At4g13660.1 68417.m02124 pinoresinol-lariciresinol reductase, pu... 27 6.4
At5g34930.1 68418.m04119 arogenate dehydrogenase identical to ar... 27 8.5
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 104 bits (249), Expect = 3e-23
Identities = 50/76 (65%), Positives = 59/76 (77%), Gaps = 1/76 (1%)
Frame = +3
Query: 246 F*GSVEV-QDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEG 422
F GS+ V D L +NG K+ V S+RDP IPW GA+YVVES+GVFTT KA++HL+G
Sbjct: 139 FKGSINVIDDSTLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKG 198
Query: 423 GAKKVIISAPSADAPM 470
GAKKVIISAPSADAPM
Sbjct: 199 GAKKVIISAPSADAPM 214
Score = 83.4 bits (197), Expect = 7e-17
Identities = 40/65 (61%), Positives = 47/65 (72%), Gaps = 1/65 (1%)
Frame = +1
Query: 94 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGVLRFR 270
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+ KYDSTHG FKG +
Sbjct: 87 TKVGINGFGRIGRLVLRIATSRDDIEVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVI 146
Query: 271 MDSLL 285
DS L
Sbjct: 147 DDSTL 151
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 101 bits (242), Expect = 2e-22
Identities = 46/69 (66%), Positives = 55/69 (79%)
Frame = +3
Query: 264 VQDGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVII 443
+ D L +NG ++ V S+RDP IPW GAEYVVES+GVFTT +AS+HL+GGAKKVII
Sbjct: 144 IDDSTLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVII 203
Query: 444 SAPSADAPM 470
SAPSADAPM
Sbjct: 204 SAPSADAPM 212
Score = 86.2 bits (204), Expect = 1e-17
Identities = 40/65 (61%), Positives = 48/65 (73%), Gaps = 1/65 (1%)
Frame = +1
Query: 94 SKIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGVLRFR 270
+K+GINGFGRIGRLVLR A+ +VVA+NDPFI YM Y+FKYDSTHG +KG +
Sbjct: 85 TKVGINGFGRIGRLVLRIATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVI 144
Query: 271 MDSLL 285
DS L
Sbjct: 145 DDSTL 149
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 95.9 bits (228), Expect = 1e-20
Identities = 45/72 (62%), Positives = 59/72 (81%), Gaps = 1/72 (1%)
Frame = +3
Query: 258 VEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKK 434
++V+D ++ G K + VF R+P+ IPWG+AGA++VVESTGVFT DKA+AHL+GGAKK
Sbjct: 63 LKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 435 VIISAPSADAPM 470
V+ISAPS DAPM
Sbjct: 123 VVISAPSKDAPM 134
Score = 78.2 bits (184), Expect = 3e-15
Identities = 38/76 (50%), Positives = 53/76 (69%), Gaps = 2/76 (2%)
Frame = +1
Query: 79 LAENMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 252
+A+ +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 253 GVLRFRMDSLLLMVTK 300
L+ + D LL K
Sbjct: 61 HELKVKDDKTLLFGEK 76
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 94.3 bits (224), Expect = 4e-20
Identities = 44/72 (61%), Positives = 58/72 (80%), Gaps = 1/72 (1%)
Frame = +3
Query: 258 VEVQDGFLVVNGNK-IAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKK 434
++++D ++ G K + VF R+P+ IPW +AGA+YVVESTGVFT DKA+AHL+GGAKK
Sbjct: 63 LKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 435 VIISAPSADAPM 470
V+ISAPS DAPM
Sbjct: 123 VVISAPSKDAPM 134
Score = 77.8 bits (183), Expect = 3e-15
Identities = 37/76 (48%), Positives = 53/76 (69%), Gaps = 2/76 (2%)
Frame = +1
Query: 79 LAENMSKIGINGFGRIGRLVLRASIEKG-AQVVAINDPFIGLDYMVYLFKYDSTHGRFK- 252
+A+ +IGINGFGRIGRLV R +++ ++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 1 MADKKIRIGINGFGRIGRLVARVVLQRDDVELVAVNDPFITTEYMTYMFKYDSVHGQWKH 60
Query: 253 GVLRFRMDSLLLMVTK 300
L+ + + LL K
Sbjct: 61 NELKIKDEKTLLFGEK 76
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 69.7 bits (163), Expect = 9e-13
Identities = 32/67 (47%), Positives = 43/67 (64%), Gaps = 1/67 (1%)
Frame = +3
Query: 270 DGFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISA 449
D L V+G I + S+R+P +PWG+ G + V+E TGVF D A HL+ GAKKV+I+A
Sbjct: 127 DSALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLITA 186
Query: 450 P-SADAP 467
P D P
Sbjct: 187 PGKGDIP 193
Score = 44.0 bits (99), Expect = 5e-05
Identities = 31/79 (39%), Positives = 41/79 (51%), Gaps = 3/79 (3%)
Frame = +1
Query: 79 LAENMSKIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRF 249
+ E K+ INGFGRIGR LR + VV IND G+ +L KYDST G F
Sbjct: 60 VTEAKIKVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTG-GVKQASHLLKYDSTLGIF 118
Query: 250 KGVLRFRMDSLLLMVTKLL 306
++ DS L + K++
Sbjct: 119 DADVKPSGDSALSVDGKII 137
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 63.7 bits (148), Expect = 6e-11
Identities = 30/62 (48%), Positives = 39/62 (62%), Gaps = 1/62 (1%)
Frame = +3
Query: 285 VNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP-SAD 461
V+G I V S R+P +PW + G + V+E TGVF + A H+E GAKKVII+AP D
Sbjct: 129 VDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVIITAPGKGD 188
Query: 462 AP 467
P
Sbjct: 189 IP 190
Score = 41.9 bits (94), Expect = 2e-04
Identities = 26/60 (43%), Positives = 33/60 (55%), Gaps = 3/60 (5%)
Frame = +1
Query: 79 LAENMSKIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRF 249
+ E K+ INGFGRIGR LR + ++AIND G+ +L KYDST G F
Sbjct: 57 VTEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTG-GVKQASHLLKYDSTLGIF 115
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 63.3 bits (147), Expect = 8e-11
Identities = 33/77 (42%), Positives = 45/77 (58%), Gaps = 3/77 (3%)
Frame = +3
Query: 246 F*GSVEVQDGFLV-VNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEG 422
F V++ D + V+G I V S RDP +PW + G + V+E TGVF A H++
Sbjct: 135 FKAEVKIVDNETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQA 194
Query: 423 GAKKVIISAPS--ADAP 467
GA KVII+AP+ AD P
Sbjct: 195 GASKVIITAPAKGADIP 211
Score = 41.5 bits (93), Expect = 3e-04
Identities = 27/73 (36%), Positives = 39/73 (53%), Gaps = 3/73 (4%)
Frame = +1
Query: 97 KIGINGFGRIGRLVLR---ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGVLRF 267
K+ INGFGRIGR LR + +VV +ND G+ +L KYDS G FK ++
Sbjct: 83 KVAINGFGRIGRNFLRCWHGRKDSPLEVVVLNDSG-GVKNASHLLKYDSMLGTFKAEVKI 141
Query: 268 RMDSLLLMVTKLL 306
+ + + KL+
Sbjct: 142 VDNETISVDGKLI 154
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 29.1 bits (62), Expect = 1.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 106 INGFGRIGRLVLRASIEKGAQVVAIND 186
I GFG +G + EKG ++VA++D
Sbjct: 211 IQGFGNVGSWAAKLISEKGGKIVAVSD 237
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 29.1 bits (62), Expect = 1.6
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +1
Query: 106 INGFGRIGRLVLRASIEKGAQVVAIND 186
I GFG +G + EKG +VVA++D
Sbjct: 211 IQGFGNVGTWAAKLIHEKGGKVVAVSD 237
>At4g01330.1 68417.m00173 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; contains
serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 329
Score = 29.1 bits (62), Expect = 1.6
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -3
Query: 207 IVKTDERVIYSDHLSSFFNRSTEHQTANAAKS 112
I KT+ RV++SD +SS +R T +TA+ + S
Sbjct: 103 IGKTEHRVVFSDRVSSGESRGTVSETASYSGS 134
>At5g24900.1 68418.m02948 cytochrome P450 family protein similar to
Cytochrome P450 72A1 (SP:Q05047) [Catharanthus roseus];
fatty acid omega-hydroxylase cytochrome P450 4A11 - Homo
sapiens, PIR:I53015
Length = 525
Score = 28.7 bits (61), Expect = 2.1
Identities = 16/74 (21%), Positives = 34/74 (45%)
Frame = +1
Query: 79 LAENMSKIGINGFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGV 258
+ + +S+ GRI + R + G ++ N P + + Y+ TH + KG+
Sbjct: 118 MVKELSQTNTLNLGRITHITKRLNPILGNGIITSNGPHWA--HQRRIIAYEFTHDKIKGM 175
Query: 259 LRFRMDSLLLMVTK 300
+ ++S + M+ K
Sbjct: 176 VGLMVESAMPMLNK 189
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 28.7 bits (61), Expect = 2.1
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 97 KIGINGFGRIGRLVLRASIEKGAQVVAIND 186
+ I GFG +G + +KG ++VA++D
Sbjct: 208 RFAIQGFGNVGSWAAKLISDKGGKIVAVSD 237
>At1g56010.2 68414.m06428 transcription activator NAC1 (NAC1)
contains Pfam PF02365: No apical meristem (NAM) domain;
identical to NAC1 GB:AAF21437 GI:6649236 from
[Arabidopsis thaliana]
Length = 324
Score = 28.7 bits (61), Expect = 2.1
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = -3
Query: 282 QGIHPEPQHSLKTAMGGIILEKINHIVKTDERVIYSDHLSSFFNRS 145
QG H P HSL + +L ++ H K E VI D++ S F+ +
Sbjct: 146 QGSHHPPNHSLSSPKEDWVLCRVFH--KNTEGVICRDNMGSCFDET 189
>At1g56010.1 68414.m06427 transcription activator NAC1 (NAC1)
contains Pfam PF02365: No apical meristem (NAM) domain;
identical to NAC1 GB:AAF21437 GI:6649236 from
[Arabidopsis thaliana]
Length = 257
Score = 28.7 bits (61), Expect = 2.1
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = -3
Query: 282 QGIHPEPQHSLKTAMGGIILEKINHIVKTDERVIYSDHLSSFFNRS 145
QG H P HSL + +L ++ H K E VI D++ S F+ +
Sbjct: 79 QGSHHPPNHSLSSPKEDWVLCRVFH--KNTEGVICRDNMGSCFDET 122
>At4g04710.1 68417.m00692 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Nicotiana tabacum] gi|3283996|gb|AAC25423;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 575
Score = 27.9 bits (59), Expect = 3.7
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +3
Query: 21 LHLVLNVRGGGQLFNKFSITCREH 92
+H+V+ + GGG+LF+K + H
Sbjct: 96 IHIVMELCGGGELFDKIDALVKSH 119
>At4g13660.1 68417.m02124 pinoresinol-lariciresinol reductase,
putative similar to pinoresinol-lariciresinol reductase
TH1 [Tsuga heterophylla][GI:7578915]; contains
isoflavone reductase domain PF02716
Length = 317
Score = 27.1 bits (57), Expect = 6.4
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +1
Query: 106 INGFGRIGRLVLRASIEKGAQVVAINDPFIGLD 204
+ G G +GR ++ A + +G + + P IG+D
Sbjct: 15 VGGTGSLGRRIVSACLAEGHETYVLQRPEIGVD 47
>At5g34930.1 68418.m04119 arogenate dehydrogenase identical to
arogenate dehydrogenase GI:16903098 from [Arabidopsis
thaliana]; contains Pfam profile: PF02153: prephenate
dehydrogenase
Length = 640
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +1
Query: 97 KIGINGFGRIGRLVLRASIEKGAQVVA 177
KIGI GFG G+ + + +++G V+A
Sbjct: 366 KIGIVGFGNFGQFLGKTMVKQGHTVLA 392
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,001,649
Number of Sequences: 28952
Number of extensions: 233035
Number of successful extensions: 720
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 714
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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