BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20770 (590 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56090.1 68418.m06998 cytochrome oxidase assembly family prot... 107 5e-24 At3g53460.2 68416.m05901 29 kDa ribonucleoprotein, chloroplast /... 29 3.1 At3g53460.1 68416.m05900 29 kDa ribonucleoprotein, chloroplast /... 29 3.1 At2g36070.1 68415.m04429 mitochondrial import inner membrane tra... 29 3.1 At5g19470.1 68418.m02320 MutT/nudix family protein similar to SP... 28 5.4 At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 27 7.1 At1g20330.1 68414.m02537 S-adenosyl-methionine-sterol-C-methyltr... 27 7.1 At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol p... 27 9.4 >At5g56090.1 68418.m06998 cytochrome oxidase assembly family protein contains PF02628: Cytochrome oxidase assembly protein Length = 457 Score = 107 bits (257), Expect = 5e-24 Identities = 49/94 (52%), Positives = 63/94 (67%) Frame = +3 Query: 228 LLFNS*TEDSKAVGYWLLGCSGMVFTAVVLGGVTRLTESGLSMVTWRLLGEKMPTNEEEW 407 LL + K VG WL G + VF+ VVLGGVTRLT SGLSM W+ GE P ++E W Sbjct: 87 LLVTGGPQAQKWVGIWLFGSAAWVFSMVVLGGVTRLTRSGLSMTDWKFTGEFPPLSDEAW 146 Query: 408 QKEFEKYQQYPEFKYKNQNITFSDFKWIWYMEFA 509 KEFEKY+Q PE+K N+ + DFK+I++ME+A Sbjct: 147 AKEFEKYKQSPEYKRVNKGMNLEDFKFIYWMEYA 180 Score = 30.7 bits (66), Expect = 0.76 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +2 Query: 509 HRTWGRAIGAAMFLPAAYFWYRGML 583 HR WGR +G LP +YF +G + Sbjct: 181 HRMWGRGLGIMFALPFSYFLRKGYI 205 >At3g53460.2 68416.m05901 29 kDa ribonucleoprotein, chloroplast / RNA-binding protein cp 29 nearly identical to SP|Q43349 29 kDa ribonucleoprotein, chloroplast precursor (RNA-binding protein cp29) {Arabidopsis thaliana} Length = 334 Score = 28.7 bits (61), Expect = 3.1 Identities = 14/30 (46%), Positives = 21/30 (70%) Frame = -2 Query: 403 SSSFVGIFSPRSLHVTIDKPDSVSRVTPPS 314 S+S + F+P+SL + + +P SVS V PPS Sbjct: 4 SASSLSAFNPKSLPLCVSRPASVS-VLPPS 32 >At3g53460.1 68416.m05900 29 kDa ribonucleoprotein, chloroplast / RNA-binding protein cp 29 nearly identical to SP|Q43349 29 kDa ribonucleoprotein, chloroplast precursor (RNA-binding protein cp29) {Arabidopsis thaliana} Length = 342 Score = 28.7 bits (61), Expect = 3.1 Identities = 14/30 (46%), Positives = 21/30 (70%) Frame = -2 Query: 403 SSSFVGIFSPRSLHVTIDKPDSVSRVTPPS 314 S+S + F+P+SL + + +P SVS V PPS Sbjct: 4 SASSLSAFNPKSLPLCVSRPASVS-VLPPS 32 >At2g36070.1 68415.m04429 mitochondrial import inner membrane translocase subunit TIM44, putative contains similarity to Swiss-Prot:O35857 import inner membrane translocase subunit TIM44, mitochondrial precursor [Mus musculus]; contains Pfam domian PF04280: Mitochondrial import inner membrane, translocase subunit TIM44 Length = 469 Score = 28.7 bits (61), Expect = 3.1 Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 4/26 (15%) Frame = +3 Query: 384 MPTNEEEWQKEF----EKYQQYPEFK 449 MPT + +WQK++ EK Q YP FK Sbjct: 237 MPTKQSKWQKKWESLREKMQGYPVFK 262 >At5g19470.1 68418.m02320 MutT/nudix family protein similar to SP|P41888 Thiamine pyrophosphokinase (EC 2.7.6.2) (TPK) (Thiamine kinase) {Schizosaccharomyces pombe}; contains Pfam profile PF00293: NUDIX domain Length = 365 Score = 27.9 bits (59), Expect = 5.4 Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 5/63 (7%) Frame = -2 Query: 421 SNSFCH-SSSFVGIFSPRSLHVTIDKPDSVSRVTPP----STTAVKTIPLHPSSQYPTAF 257 +++FC + +FS +L T+ S S +PP +V+T+PL PS + F Sbjct: 2 ASAFCSLCPTPTSLFSSHALIPTLQWRSSSSSRSPPLHISRVLSVETVPLSPSFTWNDVF 61 Query: 256 ESS 248 E+S Sbjct: 62 ENS 64 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 27.5 bits (58), Expect = 7.1 Identities = 14/44 (31%), Positives = 24/44 (54%) Frame = -2 Query: 379 SPRSLHVTIDKPDSVSRVTPPSTTAVKTIPLHPSSQYPTAFESS 248 S S +++DK S+ + PST+ V I +P +P+A S+ Sbjct: 779 SEESGKISVDKLSSIDSPSIPSTSRVLDITEYPRLNHPSAAASA 822 >At1g20330.1 68414.m02537 S-adenosyl-methionine-sterol-C-methyltransferase identical to sterol-C-methyltransferase GI:1061040 from [Arabidopsis thaliana] Length = 361 Score = 27.5 bits (58), Expect = 7.1 Identities = 19/82 (23%), Positives = 34/82 (41%), Gaps = 6/82 (7%) Frame = +3 Query: 291 GMVFTAVVLGGVTRLTESGLSMVTWRLLGEKMPTNEEE-WQ-----KEFEKYQQYPEFKY 452 G+ + VLG R + + + + EK+ N ++ W KE E ++ P+F Sbjct: 16 GIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRRPKEIETAEKVPDFVD 75 Query: 453 KNQNITFSDFKWIWYMEFATEP 518 N+ ++W W F P Sbjct: 76 TFYNLVTDIYEWGWGQSFHFSP 97 >At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol protease, putative contains similarity to cysteine proteinase RD21A (thiol protease) GI:435619, SP:P43297 from [Arabidopsis thaliana] Length = 452 Score = 27.1 bits (57), Expect = 9.4 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = +2 Query: 248 RRLKSSWILATRMQWYGLHCCSTRWRHPTDRIRFVYGHVETPRR 379 RR+ W++ R + GL R+ D ++FV H P R Sbjct: 40 RRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNR 83 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,738,191 Number of Sequences: 28952 Number of extensions: 223151 Number of successful extensions: 765 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 738 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 764 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1171109464 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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