Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20766
(736 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g25550.1 68417.m03683 expressed protein 117 9e-27
At4g29820.1 68417.m04245 expressed protein 93 2e-19
At5g63600.1 68418.m07985 flavonol synthase, putative similar to ... 32 0.45
At1g48540.2 68414.m05428 leucine-rich repeat family protein 29 2.4
At1g48540.1 68414.m05427 leucine-rich repeat family protein 29 2.4
At5g63590.1 68418.m07983 flavonol synthase, putative similar to ... 28 7.4
At5g58580.1 68418.m07341 zinc finger (C3HC4-type RING finger) fa... 27 9.8
At4g12720.3 68417.m01998 MutT/nudix family protein similar to SP... 27 9.8
At4g12720.2 68417.m01997 MutT/nudix family protein similar to SP... 27 9.8
At4g12720.1 68417.m01996 MutT/nudix family protein similar to SP... 27 9.8
At4g11330.1 68417.m01829 mitogen-activated protein kinase, putat... 27 9.8
>At4g25550.1 68417.m03683 expressed protein
Length = 200
Score = 117 bits (281), Expect = 9e-27
Identities = 48/73 (65%), Positives = 57/73 (78%)
Frame = +1
Query: 514 IGNWWRPNFEPPQYPYIPPHITKPKEHKRLFLVQLQDRALFAVPKNYKLVAAPLFELYDN 693
+ WWRPNFE YPY PPHITKPKE KRL++V L ++ FAVPKN KL+A PLFELYDN
Sbjct: 116 VATWWRPNFETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDN 175
Query: 694 AQGYGPIISSLSQ 732
Q YGP+IS++ Q
Sbjct: 176 VQRYGPVISTIPQ 188
Score = 94.7 bits (225), Expect = 6e-20
Identities = 45/72 (62%), Positives = 51/72 (70%)
Frame = +2
Query: 257 SVPARFQRMREEFCKIGMRRSVEGVLLVHEHGLPHVLLLQLGTAFFKLPGGELNPGEDEI 436
SV R RM+ + K GMR SVEG+LLV EH PH+LLLQ+G F KLPGG L PGE+E
Sbjct: 29 SVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHILLLQIGNTFCKLPGGRLKPGENEA 88
Query: 437 DGLKRLLTETLG 472
DGLKR LT LG
Sbjct: 89 DGLKRKLTSKLG 100
Score = 41.5 bits (93), Expect = 6e-04
Identities = 15/27 (55%), Positives = 22/27 (81%)
Frame = +3
Query: 174 LTLNRSINLYPLTNYTFGTKEPLFEKD 254
+ +++ +N YPL+NY+FGTKEP EKD
Sbjct: 1 MAMSQVVNTYPLSNYSFGTKEPKLEKD 27
>At4g29820.1 68417.m04245 expressed protein
Length = 222
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/71 (54%), Positives = 52/71 (73%)
Frame = +1
Query: 514 IGNWWRPNFEPPQYPYIPPHITKPKEHKRLFLVQLQDRALFAVPKNYKLVAAPLFELYDN 693
IG WWRPNFE YP++PP+I PKE +LFLV+L F VPKN+KL+A PL +L++N
Sbjct: 138 IGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVVPKNFKLLAVPLCQLHEN 197
Query: 694 AQGYGPIISSL 726
+ YGPI+S +
Sbjct: 198 EKTYGPIMSQI 208
Score = 69.3 bits (162), Expect = 2e-12
Identities = 35/73 (47%), Positives = 43/73 (58%)
Frame = +2
Query: 269 RFQRMREEFCKIGMRRSVEGVLLVHEHGLPHVLLLQLGTAFFKLPGGELNPGEDEIDGLK 448
R R++ + G+R VE VLLV PHVLLLQ + FKLPGG L PGE +I+GLK
Sbjct: 55 RVIRLKSNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLK 114
Query: 449 RLLTETLGRQDGV 487
R L L + V
Sbjct: 115 RKLASKLSVNENV 127
Score = 30.3 bits (65), Expect = 1.4
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +3
Query: 192 INLYPLTNYTFGTKEPLFEKD 254
++LYPL++Y FG+KE L KD
Sbjct: 29 VDLYPLSSYYFGSKEALRVKD 49
>At5g63600.1 68418.m07985 flavonol synthase, putative similar to
SP|Q96330; contains Pfam profile PF03171: 2OG-Fe(II)
oxygenase superfamily
Length = 325
Score = 31.9 bits (69), Expect = 0.45
Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 5/47 (10%)
Frame = +2
Query: 326 GVLLVHEHGLPHVLLLQL---GTAFFKLPGGELN--PGEDEIDGLKR 451
GV V HG+P L+ QL GT FF+LP E E++ +G K+
Sbjct: 58 GVFQVVNHGIPTELMRQLQMVGTQFFELPDAEKETVAKEEDFEGYKK 104
>At1g48540.2 68414.m05428 leucine-rich repeat family protein
Length = 1051
Score = 29.5 bits (63), Expect = 2.4
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = -3
Query: 137 SWSRRPLFVTGRPLNCCHLIFDLIFNKTAYA 45
SW R LFV GR L C F + ++TAY+
Sbjct: 954 SWLLRSLFVAGRRLFICSEDFTQLSSRTAYS 984
>At1g48540.1 68414.m05427 leucine-rich repeat family protein
Length = 1063
Score = 29.5 bits (63), Expect = 2.4
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = -3
Query: 137 SWSRRPLFVTGRPLNCCHLIFDLIFNKTAYA 45
SW R LFV GR L C F + ++TAY+
Sbjct: 954 SWLLRSLFVAGRRLFICSEDFTQLSSRTAYS 984
>At5g63590.1 68418.m07983 flavonol synthase, putative similar to
SP|Q96330 Flavonol synthase 1 (EC 1.14.11.-) (FLS 1)
{Arabidopsis thaliana}; contains Pfam profile PF03171:
oxidoreductase, 2OG-Fe(II) oxygenase family
Length = 308
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 3/32 (9%)
Frame = +2
Query: 326 GVLLVHEHGLPHVL---LLQLGTAFFKLPGGE 412
G+ V HG+P L LLQ+G FF+LP E
Sbjct: 39 GIFQVVNHGIPTELILRLLQVGMEFFELPETE 70
>At5g58580.1 68418.m07341 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 308
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = -1
Query: 316 PSHSDFTKLLSHPLEPRRDRRSFSNSGSLVPNV 218
PSH DF+ H RR+RR + ++P++
Sbjct: 57 PSHQDFSAAARHRRRRRRNRRRTVTTTRIIPSL 89
>At4g12720.3 68417.m01998 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +2
Query: 326 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 472
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g12720.2 68417.m01997 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +2
Query: 326 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 472
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g12720.1 68417.m01996 MutT/nudix family protein similar to
SP|P53370 Nucleoside diphosphate-linked moiety X motif 6
{Homo sapiens}; contains Pfam profile PF00293: NUDIX
domain
Length = 282
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 6/55 (10%)
Frame = +2
Query: 326 GVLLVHEHGLPHVLLLQLGTAFFK------LPGGELNPGEDEIDGLKRLLTETLG 472
G L+++++ VL++Q + FFK LP G +N GED G+ R + E G
Sbjct: 107 GALVINKN-TKEVLVVQERSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETG 160
>At4g11330.1 68417.m01829 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK5) similar to mitogen-activated
protein kinase homolog 5 (AtMPK5)[Arabidopsis thaliana]
SWISS-PROT:Q39025; PMID:12119167; possible internal
deletion at position 161, missing one A residue;
reference GI:457401
Length = 250
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = +2
Query: 413 LNPGEDEIDGLKRLLTETLGRQDGVKQEWL 502
L PG+D + LK L+TE +G DG E+L
Sbjct: 117 LFPGKDYVHQLK-LITELIGSPDGASLEFL 145
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,400,708
Number of Sequences: 28952
Number of extensions: 347486
Number of successful extensions: 1001
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1001
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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