Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20746
(778 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 135 3e-32
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 73 2e-13
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 73 2e-13
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 73 2e-13
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 64 7e-11
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 36 0.040
At3g56570.1 68416.m06290 SET domain-containing protein low simil... 33 0.21
At2g41620.1 68415.m05143 nucleoporin interacting component famil... 31 1.1
At1g24050.1 68414.m03036 expressed protein 30 1.5
At4g19590.1 68417.m02879 DNAJ heat shock N-terminal domain-conta... 29 2.6
At2g47500.1 68415.m05929 kinesin motor protein-related 29 2.6
At5g59740.1 68418.m07488 UDP-galactose/UDP-glucose transporter-r... 29 4.5
At3g46180.1 68416.m04997 UDP-galactose/UDP-glucose transporter-r... 28 6.0
At4g14060.1 68417.m02171 major latex protein-related / MLP-relat... 28 7.9
At2g01520.1 68415.m00076 major latex protein-related / MLP-relat... 28 7.9
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 135 bits (327), Expect = 3e-32
Identities = 58/88 (65%), Positives = 71/88 (80%)
Frame = +2
Query: 254 VIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYA 433
++ HLPSP AQ+YR+E LYEGP DD+ A I++CDP PLM+YVSKM+P SDKGRF+A
Sbjct: 335 MMIFHLPSPHTAQRYRVENLYEGPLDDQYANAIRNCDPNGPLMLYVSKMIPASDKGRFFA 394
Query: 434 FGRVFSGKVVTGQKARIMGPNFTPGRKR 517
FGRVF+GKV TG K RIMGPN+ PG K+
Sbjct: 395 FGRVFAGKVSTGMKVRIMGPNYIPGEKK 422
Score = 109 bits (262), Expect = 2e-24
Identities = 53/92 (57%), Positives = 70/92 (76%), Gaps = 2/92 (2%)
Frame = +1
Query: 508 KKEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFK--NAHNMK 681
+K+DLY K++QRT++ MG+ E +EDVP GN +VG+DQF+ K T+T K +AH ++
Sbjct: 420 EKKDLYTKSVQRTVIWMGKRQETVEDVPCGNTVAMVGLDQFITKNATLTNEKEVDAHPIR 479
Query: 682 VMKFSVSPVVRVAVEPKNPADLPKLVEVLKRL 777
MKFSVSPVVRVAV+ K +DLPKLVE LKRL
Sbjct: 480 AMKFSVSPVVRVAVQCKVASDLPKLVEGLKRL 511
Score = 70.1 bits (164), Expect = 2e-12
Identities = 33/90 (36%), Positives = 57/90 (63%), Gaps = 2/90 (2%)
Frame = +3
Query: 3 FFNPQTKKWSKQKDDDN--KRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHE 176
FF+P T+KWS + KR F + +PI ++ M +K+++ +L K+GV++K++
Sbjct: 249 FFDPATRKWSGKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPMLAKLGVSMKND 308
Query: 177 DSDKDGKALLKVVMRSWLPAGEALLQ*LPF 266
+ + GK L+K VM++WLPA ALL+ + F
Sbjct: 309 EKELMGKPLMKRVMQTWLPASTALLEMMIF 338
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/80 (40%), Positives = 47/80 (58%)
Frame = +2
Query: 266 HLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRV 445
H+PSP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV
Sbjct: 437 HIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRV 496
Query: 446 FSGKVVTGQKARIMGPNFTP 505
+SG++ TGQ R++G ++P
Sbjct: 497 YSGRLQTGQSVRVLGEGYSP 516
Score = 59.7 bits (138), Expect = 2e-09
Identities = 29/92 (31%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
Frame = +1
Query: 511 KEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMK 681
+ED+ K + + + RY + P G+ + GVD ++KT T+ ++ + +
Sbjct: 519 EEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFR 578
Query: 682 VMKFSVSPVVRVAVEPKNPADLPKLVEVLKRL 777
+KF+ PVV+ A EP NP++LPK+VE L+++
Sbjct: 579 ALKFNTLPVVKTATEPLNPSELPKMVEGLRKI 610
Score = 40.7 bits (91), Expect = 0.001
Identities = 16/75 (21%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +3
Query: 3 FFNPQTKKWSKQKD-DDNKRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHED 179
+++P T+ ++ +R+F ++L+P+YK++ ++ K+ ++ L ++GVT+ +
Sbjct: 348 YYHPDTRVFNTSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSA 407
Query: 180 SDKDGKALLKVVMRS 224
+ + LL++ S
Sbjct: 408 YKLNVRPLLRLACSS 422
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/80 (40%), Positives = 47/80 (58%)
Frame = +2
Query: 266 HLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRV 445
H+PSP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV
Sbjct: 451 HIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRV 510
Query: 446 FSGKVVTGQKARIMGPNFTP 505
+SG++ TGQ R++G ++P
Sbjct: 511 YSGRLQTGQSVRVLGEGYSP 530
Score = 58.0 bits (134), Expect = 6e-09
Identities = 28/92 (30%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
Frame = +1
Query: 511 KEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMK 681
+ED+ K + + + RY + P G+ + GVD ++KT T+ ++ + +
Sbjct: 533 EEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFR 592
Query: 682 VMKFSVSPVVRVAVEPKNPADLPKLVEVLKRL 777
++F+ PVV+ A EP NP++LPK+VE L+++
Sbjct: 593 ALQFNTLPVVKTATEPLNPSELPKMVEGLRKI 624
Score = 38.7 bits (86), Expect = 0.004
Identities = 14/57 (24%), Positives = 35/57 (61%)
Frame = +3
Query: 54 KRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHEDSDKDGKALLKVVMRS 224
+R+F ++L+P+YK++ ++ K+ ++ L ++GVT+ + + + LL++ S
Sbjct: 380 ERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSS 436
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 73.3 bits (172), Expect = 2e-13
Identities = 32/80 (40%), Positives = 47/80 (58%)
Frame = +2
Query: 266 HLPSPVVAQKYRMEMLYEGPHDDEAAIGIKSCDPEAPLMMYVSKMVPTSDKGRFYAFGRV 445
H+PSP A +++ Y G D + CDP PLM+ V+K+ P SD F FGRV
Sbjct: 451 HIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRV 510
Query: 446 FSGKVVTGQKARIMGPNFTP 505
+SG++ TGQ R++G ++P
Sbjct: 511 YSGRLQTGQSVRVLGEGYSP 530
Score = 58.0 bits (134), Expect = 6e-09
Identities = 28/92 (30%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
Frame = +1
Query: 511 KEDLYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTF---KNAHNMK 681
+ED+ K + + + RY + P G+ + GVD ++KT T+ ++ + +
Sbjct: 533 EEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFR 592
Query: 682 VMKFSVSPVVRVAVEPKNPADLPKLVEVLKRL 777
++F+ PVV+ A EP NP++LPK+VE L+++
Sbjct: 593 ALQFNTLPVVKTATEPLNPSELPKMVEGLRKI 624
Score = 38.7 bits (86), Expect = 0.004
Identities = 14/57 (24%), Positives = 35/57 (61%)
Frame = +3
Query: 54 KRSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHEDSDKDGKALLKVVMRS 224
+R+F ++L+P+YK++ ++ K+ ++ L ++GVT+ + + + LL++ S
Sbjct: 380 ERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSS 436
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 64.5 bits (150), Expect = 7e-11
Identities = 28/86 (32%), Positives = 54/86 (62%)
Frame = +1
Query: 520 LYEKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKNAHNMKVMKFSV 699
+ E + LMMG+ + + +V +GN+ + G+ ++ K+ T+++ +N + M+F V
Sbjct: 476 IQEAELHSLYLMMGQGLTPVTEVKAGNVVAIRGLGPYISKSATLSSTRNCWPLASMEFQV 535
Query: 700 SPVVRVAVEPKNPADLPKLVEVLKRL 777
SP +RVA+EP +PAD+ L++ L+ L
Sbjct: 536 SPTLRVAIEPSDPADMSALMKGLRLL 561
Score = 41.1 bits (92), Expect = 8e-04
Identities = 22/69 (31%), Positives = 41/69 (59%), Gaps = 3/69 (4%)
Frame = +3
Query: 54 KRSFCMYVLDPIYKVFDAIMK--FKKEEIDDLLKKIGVTIK-HEDSDKDGKALLKVVMRS 224
K F +VL+P+++V++A + K ++ ++K ++I E +KD K +L+ VM
Sbjct: 272 KPMFVQFVLEPLWQVYEAALDPGGDKAVLEKVIKSFNLSIPPRELQNKDPKNVLQSVMSR 331
Query: 225 WLPAGEALL 251
WLP +A+L
Sbjct: 332 WLPLSDAVL 340
Score = 27.1 bits (57), Expect(2) = 0.17
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 16/66 (24%)
Frame = +2
Query: 251 SVIAIHLPSPVVAQKYRMEMLYE-----GPHDDEAAI---------GIKSCD--PEAPLM 382
S+ HLP P+ AQ YR+ L G D ++++ I++CD ++P +
Sbjct: 341 SMAVKHLPDPIAAQAYRIPRLVPERKIIGGDDVDSSVLAEAELVRKSIEACDSSSDSPCV 400
Query: 383 MYVSKM 400
++VSKM
Sbjct: 401 VFVSKM 406
Score = 25.0 bits (52), Expect(2) = 0.17
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 425 FYAFGRVFSGKVVTGQKARIMGPNFTP 505
F AF R+FSG + GQ+ ++ + P
Sbjct: 440 FLAFARIFSGVLRAGQRVFVITALYDP 466
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 35.5 bits (78), Expect = 0.040
Identities = 26/85 (30%), Positives = 46/85 (54%), Gaps = 1/85 (1%)
Frame = +1
Query: 526 EKTIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTG-TITTFKNAHNMKVMKFSVS 702
++ I R + M E ++ +G+I L G+ + TG T++ +N ++ M F
Sbjct: 436 KERIGRLLEMHANSREDVKVALTGDIIALAGLKDTI--TGETLSDPENPVVLERMDFP-D 492
Query: 703 PVVRVAVEPKNPADLPKLVEVLKRL 777
PV++VA+EPK AD+ K+ L +L
Sbjct: 493 PVIKVAIEPKTKADIDKMATGLIKL 517
>At3g56570.1 68416.m06290 SET domain-containing protein low
similarity to SP|Q43088 Ribulose-1,5 bisphosphate
carboxylase/oxygenase large subunit N-
methyltransferase, chloroplast precursor (EC 2.1.1.127)
{Pisum sativum}; contains Pfam profile PF00856: SET
domain
Length = 531
Score = 33.1 bits (72), Expect = 0.21
Identities = 17/40 (42%), Positives = 22/40 (55%)
Frame = -3
Query: 338 LHHHGVPRITSPYDISGPLQVMVNEWQSLKQSFTSRQPRA 219
LH +G + +PYDI +V EW + SFTSR RA
Sbjct: 319 LHRYGFTELDNPYDIVNIDLELVTEWST--SSFTSRYTRA 356
>At2g41620.1 68415.m05143 nucleoporin interacting component family
protein contains Pfam profile PF04097: Nucleoporin
interacting component
Length = 861
Score = 30.7 bits (66), Expect = 1.1
Identities = 18/68 (26%), Positives = 32/68 (47%)
Frame = -1
Query: 499 KVWSHDASFLSGNNLARENASKGVETTLVGGRHHLAHVHHQWGFRITALDTNGSFIIMGS 320
K+W + ++ R+ SK + +G RHHL H H + F + + T+ + +G
Sbjct: 251 KIWQLVQAITGEDSAVRQGVSKRMALA-IGARHHLQHGHEK--FIMDTIQTHPTQAALGG 307
Query: 319 LV*HLHTI 296
V +L I
Sbjct: 308 SVGNLQRI 315
>At1g24050.1 68414.m03036 expressed protein
Length = 173
Score = 30.3 bits (65), Expect = 1.5
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 416 KGRFYAFGRVFSGKVVTGQKARIMGPNFTPGR-KRTCMRRLSSVQSL 553
+G +A G ++S KV+TG + R +G PG K T M S + L
Sbjct: 15 EGEKFAVGNIYSVKVITGDEFRGIGTKPRPGHLKNTRMVNASFITGL 61
>At4g19590.1 68417.m02879 DNAJ heat shock N-terminal
domain-containing protein protein YJL162c, Saccharomyces
cerevisiae, PIR2:S56945; contains Pfam PF00226: DnaJ
domain;
Length = 345
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/55 (27%), Positives = 33/55 (60%), Gaps = 4/55 (7%)
Frame = +1
Query: 472 KSSHHGTKLYTWKKEDLYEKT--IQRTILMMGRYVEAIEDVPSG--NICGLVGVD 624
++ ++G KL+ K +DLY K +++ ++++ Y+ A + G + G++GVD
Sbjct: 10 ENDYNGAKLFANKAQDLYPKLDGLRQVMMLIDVYISAGNTISGGESDWYGILGVD 64
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 29.5 bits (63), Expect = 2.6
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 661 KNAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEVL 768
+N N S+S +VR + K P D+PKL+E L
Sbjct: 218 ENDSNKLSSPSSLSTLVRAVLSDKKPEDVPKLIESL 253
>At5g59740.1 68418.m07488 UDP-galactose/UDP-glucose
transporter-related weak similarity to
UDP-galactose/UDP-glucose transporter [Arabidopsis
thaliana] GI:22651763
Length = 344
Score = 28.7 bits (61), Expect = 4.5
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -2
Query: 600 VTRGHILNSFNITSHHKDCTLDSLLIQVL 514
+ +GH+L + + S H+DC LD L+ +
Sbjct: 228 ILQGHLLPAVDFVSLHRDCLLDIALLSTV 256
>At3g46180.1 68416.m04997 UDP-galactose/UDP-glucose
transporter-related contains weak similarity to
UDP-galactose/UDP-glucose transporter (GI:22651763)
[Arabidopsis thaliana]
Length = 347
Score = 28.3 bits (60), Expect = 6.0
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = -2
Query: 600 VTRGHILNSFNITSHHKDCTLDSLLIQVL 514
+ +GH+L + + S H+DC D L+ +
Sbjct: 229 ILQGHLLPAVDFVSRHRDCLFDIALLSTV 257
>At4g14060.1 68417.m02171 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:294060] ; contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 7.9
Identities = 20/73 (27%), Positives = 40/73 (54%), Gaps = 5/73 (6%)
Frame = +3
Query: 12 PQTKKWSKQKDDDNKRSFCM----YVLDPIYKVFDAIMKFKKEEIDDLLKKIGVT-IKHE 176
P+ K ++ DD+N + +V++ + KV+D I++F ++ DD++ KI +T K
Sbjct: 66 PEMFKERREIDDENMAVTFVGLEGHVMEQL-KVYDTILQFIQKSPDDIVCKITMTWEKRA 124
Query: 177 DSDKDGKALLKVV 215
D + +K+V
Sbjct: 125 DDSPEPSNYMKLV 137
>At2g01520.1 68415.m00076 major latex protein-related / MLP-related
low similarity to major latex protein {Papaver
somniferum}[GI:169000] contains Pfam profile PF00407:
Pathogenesis-related protein Bet v I family
Length = 151
Score = 27.9 bits (59), Expect = 7.9
Identities = 17/62 (27%), Positives = 35/62 (56%), Gaps = 4/62 (6%)
Frame = +3
Query: 12 PQTKKWSKQKDDDNK----RSFCMYVLDPIYKVFDAIMKFKKEEIDDLLKKIGVTIKHED 179
P+ K ++ DD+N R +V++ + KV+D I +F ++ DD++ KI + + ++
Sbjct: 66 PEVFKERREIDDENMAVTFRGLEGHVMEQL-KVYDVIFQFIQKSPDDIICKITMIWEKQN 124
Query: 180 SD 185
D
Sbjct: 125 DD 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,317,254
Number of Sequences: 28952
Number of extensions: 410432
Number of successful extensions: 1257
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1252
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1736283200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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