Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20744
         (719 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   138   6e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    90   3e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    89   8e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    69   5e-13
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.7  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.6  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        26   4.7  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    26   4.7  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    26   6.2  
SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein Pop2|Schizosacchar...    25   8.2  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   8.2  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  138 bits (335), Expect = 6e-34
 Identities = 61/77 (79%), Positives = 70/77 (90%), Gaps = 1/77 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
           PR +LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 426 RKEAESCDCLQGFQLTH 476
           R+EAE+CD LQGFQLTH
Sbjct: 121 RREAEACDALQGFQLTH 137



 Score =  117 bits (282), Expect = 1e-27
 Identities = 54/70 (77%), Positives = 62/70 (88%)
 Frame = +2

Query: 506 GTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 685
           GTLL+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207

Query: 686 YDICFRTLKL 715
             I   TLK+
Sbjct: 208 SSIFANTLKI 217



 Score =  112 bits (270), Expect = 4e-26
 Identities = 47/63 (74%), Positives = 54/63 (85%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 246
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 247 PRA 255
           PRA
Sbjct: 61  PRA 63


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 89.8 bits (213), Expect = 3e-19
 Identities = 39/77 (50%), Positives = 55/77 (71%), Gaps = 1/77 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
           PR I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++DSVL+ +
Sbjct: 67  PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126

Query: 426 RKEAESCDCLQGFQLTH 476
           R+ A++C  LQGF + H
Sbjct: 127 RRMADNCSGLQGFLVFH 143



 Score = 74.5 bits (175), Expect = 1e-14
 Identities = 32/70 (45%), Positives = 48/70 (68%)
 Frame = +2

Query: 506 GTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 685
           G LL+ ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++N+D T+ +DNEA 
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEAC 213

Query: 686 YDICFRTLKL 715
           YDIC R L +
Sbjct: 214 YDICRRNLDI 223



 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 6/69 (8%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 228
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 229 SGGKYVPRA 255
             GK+VPR+
Sbjct: 61  GQGKFVPRS 69


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 88.6 bits (210), Expect = 8e-19
 Identities = 39/77 (50%), Positives = 53/77 (68%), Gaps = 1/77 (1%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
           PR I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D +
Sbjct: 63  PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122

Query: 426 RKEAESCDCLQGFQLTH 476
           R+ A++C  LQGF + H
Sbjct: 123 RRIADNCSGLQGFLVFH 139



 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 30/71 (42%), Positives = 47/71 (66%)
 Frame = +2

Query: 503 YGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 682
           +G LL+ ++  EY  +    +SV P+P+VS +VVEPYN+ L+ H  ++  D T+ +DNE+
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208

Query: 683 LYDICFRTLKL 715
            YDIC R L +
Sbjct: 209 CYDICRRNLDI 219



 Score = 55.6 bits (128), Expect = 7e-09
 Identities = 27/65 (41%), Positives = 37/65 (56%), Gaps = 2/65 (3%)
 Frame = +1

Query: 67  MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 240
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 241 YVPRA 255
           YVPR+
Sbjct: 61  YVPRS 65


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 69.3 bits (162), Expect = 5e-13
 Identities = 30/79 (37%), Positives = 52/79 (65%), Gaps = 3/79 (3%)
 Frame = +3

Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLD 419
           PR IL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D
Sbjct: 62  PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120

Query: 420 VVRKEAESCDCLQGFQLTH 476
           ++ +EA+  D L+GF L H
Sbjct: 121 MIDREADGSDSLEGFSLLH 139



 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 27/62 (43%), Positives = 43/62 (69%)
 Frame = +1

Query: 70  REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 249
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 250 RA 255
           RA
Sbjct: 63  RA 64



 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 28/64 (43%), Positives = 41/64 (64%), Gaps = 1/64 (1%)
 Frame = +2

Query: 506 GTLLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 682
           G+ L+ ++ + YP +I+ TYSV P S  VSD VV+PYN+ L++ +L  N D    +DN A
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAA 209

Query: 683 LYDI 694
           L  I
Sbjct: 210 LAHI 213


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +1

Query: 106 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 207
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 300 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 401
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +3

Query: 369 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 458
           A+GH   G ELV +  D +RK++E+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -2

Query: 442 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 305
           ++A+ RTTS T+    +PS       L P P    +  S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +3

Query: 321  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 452
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


>SPAC4D7.03 |pop2|sud1|F-box/WD repeat protein
           Pop2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 703

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 19/66 (28%), Positives = 28/66 (42%)
 Frame = +2

Query: 479 ARRRHRFRYGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDE 658
           A+  H FR    +I  +    P R++     +  P     V    + TL V +L +NTD 
Sbjct: 421 AKCTHIFRGHISIIRCLEILVPSRLIRHGVEIVEPDQPYIVSGSRDHTLRVWKLPKNTDP 480

Query: 659 TYCIDN 676
            Y  DN
Sbjct: 481 PYLPDN 486


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -2

Query: 358 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 263
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,781,087
Number of Sequences: 5004
Number of extensions: 54135
Number of successful extensions: 174
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)