Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20730
(478 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 139 2e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 90 1e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 89 3e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 76 2e-15
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.1
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.5
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 1.9
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 2.6
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 4.5
SPAC2E1P5.05 |||U3 snoRNP-associated protein Rrp9 |Schizosacchar... 25 5.9
SPBC2F12.13 |klp5|sot1|kinesin-like protein Klp5|Schizosaccharom... 25 5.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 139 bits (336), Expect = 2e-34
Identities = 59/75 (78%), Positives = 69/75 (92%)
Frame = +1
Query: 253 LEPGTMESVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
LEPGTM++V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+C
Sbjct: 68 LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127
Query: 433 DCLQGFQLTHSLGGG 477
D LQGFQLTHSLGGG
Sbjct: 128 DALQGFQLTHSLGGG 142
Score = 120 bits (290), Expect = 9e-29
Identities = 50/70 (71%), Positives = 59/70 (84%)
Frame = +2
Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 232
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 233 PRAILVDWSP 262
PRA+LVD P
Sbjct: 61 PRAVLVDLEP 70
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 90.2 bits (214), Expect = 1e-19
Identities = 36/75 (48%), Positives = 54/75 (72%)
Frame = +1
Query: 253 LEPGTMESVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
LEP ++ VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 74 LEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNC 133
Query: 433 DCLQGFQLTHSLGGG 477
LQGF + HS GGG
Sbjct: 134 SGLQGFLVFHSFGGG 148
Score = 56.0 bits (129), Expect = 3e-09
Identities = 28/76 (36%), Positives = 40/76 (52%), Gaps = 6/76 (7%)
Frame = +2
Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 214
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 215 SGGKYVPRAILVDWSP 262
GK+VPR+I VD P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 89.0 bits (211), Expect = 3e-19
Identities = 36/75 (48%), Positives = 52/75 (69%)
Frame = +1
Query: 253 LEPGTMESVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESC 432
LEP ++ VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C
Sbjct: 70 LEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNC 129
Query: 433 DCLQGFQLTHSLGGG 477
LQGF + HS GGG
Sbjct: 130 SGLQGFLVFHSFGGG 144
Score = 62.1 bits (144), Expect = 4e-11
Identities = 30/72 (41%), Positives = 41/72 (56%), Gaps = 2/72 (2%)
Frame = +2
Query: 53 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 226
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 227 YVPRAILVDWSP 262
YVPR+I VD P
Sbjct: 61 YVPRSIYVDLEP 72
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 76.2 bits (179), Expect = 2e-15
Identities = 32/69 (46%), Positives = 48/69 (69%)
Frame = +2
Query: 56 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 235
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 236 RAILVDWSP 262
RAIL+D P
Sbjct: 63 RAILIDLEP 71
Score = 67.7 bits (158), Expect = 9e-13
Identities = 27/77 (35%), Positives = 49/77 (63%), Gaps = 2/77 (2%)
Frame = +1
Query: 253 LEPGTMESVRSGPFGQIFRPDNFVFGQSG--AGNNWAKGHYTEGAELVDSVLDVVRKESE 426
LEP + ++ S +G ++ P+N + ++G AGNNWA G Y+ + + ++D++ +E++
Sbjct: 69 LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127
Query: 427 SCDCLQGFQLTHSLGGG 477
D L+GF L HS+ GG
Sbjct: 128 GSDSLEGFSLLHSIAGG 144
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.1
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 355 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 444
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 92 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 193
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 1.9
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 286 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 387
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 2.6
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3
Query: 36 KITYTK*GKSFIYRPANVV 92
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 4.5
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 307 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 438
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC2E1P5.05 |||U3 snoRNP-associated protein Rrp9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 524
Score = 25.0 bits (52), Expect = 5.9
Identities = 15/57 (26%), Positives = 23/57 (40%)
Frame = -2
Query: 399 DRVNELSTLCVVSLGPVVAGAGLSEDEVVRTEDLSERSGADRFHGAGLQSTRMARGT 229
D V E++ L + + G + GL V+R E + H + M RGT
Sbjct: 206 DHVKEITCLAISNDGRWIVTGGLDHRIVIRDSVTLEPQHCWKHHRDAVMGLAMRRGT 262
>SPBC2F12.13 |klp5|sot1|kinesin-like protein
Klp5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 883
Score = 25.0 bits (52), Expect = 5.9
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = -2
Query: 339 AGLSEDEVVRTEDLSERSGADRFHGAGLQSTRMARG 232
AG++ED + T + + +G++R L+ +R+ G
Sbjct: 279 AGINEDHTLATLSIIDLAGSERATATKLRGSRLFEG 314
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,840,709
Number of Sequences: 5004
Number of extensions: 32830
Number of successful extensions: 94
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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