BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20728 (699 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo... 32 0.069 SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.64 SPBP19A11.07c ||SPBP4H10.02c|human down-regulated in multiple ca... 26 6.0 SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyc... 26 6.0 SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual 26 6.0 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 25 7.9 >SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharomyces pombe|chr 2|||Manual Length = 840 Score = 32.3 bits (70), Expect = 0.069 Identities = 24/101 (23%), Positives = 53/101 (52%), Gaps = 7/101 (6%) Frame = +2 Query: 167 KLDAVYRSILLTKFFTKGWGKPENLR-RLFGSEKLYRIV---KNASNWLKETIPSPLLRN 334 K DAVYR+ ++ K+F + K N++ R++ + + YR++ + + W ++T R Sbjct: 193 KADAVYRAFIMLKYFPRWVQKLRNIQSRIYSAGEAYRLILIGQMVNRWRQKT------RE 246 Query: 335 KMSPRASFLKDTSL--VLLR-NTFQESYQR*HKRHISKHCY 448 ++ + F K+ L VLL+ + ++ R H+ + + + Sbjct: 247 RLHQKLRFQKNAFLLDVLLKWSALTVNWNRKHEYMLKRRVF 287 >SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1073 Score = 29.1 bits (62), Expect = 0.64 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = -2 Query: 503 VPAK*RQTGLQSLSGHFVGSSAWKCAFCAIS-GTIPGRCFSRGLVKYPSRSLPS 345 +PA+ R L LS HF+ S + + + I T G+C+ LVK P ++ P+ Sbjct: 53 LPARTRSVAL--LSSHFLQSESGRHSIANILIATENGKCYLLQLVKTPEKAFPT 104 >SPBP19A11.07c ||SPBP4H10.02c|human down-regulated in multiple cancers-1 homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 25.8 bits (54), Expect = 6.0 Identities = 23/91 (25%), Positives = 39/91 (42%) Frame = +2 Query: 14 CSITKITYFKFNQSFLFQPIC*RNKLGNQSYNFNLKTILFSIKIT*RMSASKLDAVYRSI 193 C I K + + S F +C + YN + ILFSI +AS + + + Sbjct: 423 CHIDK-SLISPSPSSQFPVLCTLENPSSIEYNI-ISNILFSIPTIPLKNASPIVELISYV 480 Query: 194 LLTKFFTKGWGKPENLRRLFGSEKLYRIVKN 286 + +FF K + ++L S LY ++ N Sbjct: 481 MKPEFFMKSQQNASDCKKLL-SSFLYFLINN 510 >SPBC18H10.16 |||amino acid permease, unknown 9|Schizosaccharomyces pombe|chr 2|||Manual Length = 1050 Score = 25.8 bits (54), Expect = 6.0 Identities = 16/45 (35%), Positives = 23/45 (51%) Frame = -2 Query: 464 SGHFVGSSAWKCAFCAISGTIPGRCFSRGLVKYPSRSLPSATFCS 330 +G+ S + F A +G + G S G +K PSRS+P T S Sbjct: 287 TGYESFKSTFGVFFPATAGLLAGASMS-GDLKAPSRSIPKGTISS 330 >SPAC343.06c |||scramblase|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = -1 Query: 243 RKFSGLPQPFVKNFVNRILRYTASN 169 R F GLP+ F + N +LR+T+++ Sbjct: 231 RNFMGLPREFFTDTGNYVLRFTSTS 255 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/21 (42%), Positives = 15/21 (71%) Frame = -2 Query: 662 HKYIRDIVERRTDLINRFTKS 600 H Y D++ +RTD++N F +S Sbjct: 828 HGYGNDVLVQRTDVVNNFGES 848 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,003,826 Number of Sequences: 5004 Number of extensions: 65258 Number of successful extensions: 189 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 189 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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