BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20728 (699 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12150.1 68416.m01514 expressed protein 42 3e-04 At3g48690.1 68416.m05317 expressed protein similar to PrMC3 [Pin... 32 0.42 At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pin... 29 2.2 At3g09270.1 68416.m01101 glutathione S-transferase, putative sim... 28 6.8 At4g32650.2 68417.m04648 inward rectifying potassium channel, pu... 27 9.0 At4g32650.1 68417.m04647 inward rectifying potassium channel, pu... 27 9.0 At3g25600.1 68416.m03187 calmodulin, putative similar to calmodu... 27 9.0 >At3g12150.1 68416.m01514 expressed protein Length = 363 Score = 42.3 bits (95), Expect = 3e-04 Identities = 26/60 (43%), Positives = 36/60 (60%) Frame = +3 Query: 510 HFYWRRRNLMVKPLLKEAGIGGIILENPFYGLRKPIDQIRSSLHNVSDIFVMGGCLILES 689 H Y RR L PL+K+ I ++LE+PFYG R+P Q + L VSD+ ++G I ES Sbjct: 134 HTYDRRLRLG-GPLVKQ-NIATMVLESPFYGQRRPFLQCGARLLCVSDLLLLGRATIEES 191 Score = 29.1 bits (62), Expect = 3.0 Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 2/53 (3%) Frame = +2 Query: 179 VYRSILLTKFFTKGWGKP--ENLRRLFGSEKLYRIVKNASNWLKETIPSPLLR 331 ++R+ + FF++GWG P E L R+ ++L+ + NW P PL+R Sbjct: 24 MHRTKMTPPFFSRGWGGPNLELLERMV--QRLFPLEVQGQNW-----PPPLVR 69 >At3g48690.1 68416.m05317 expressed protein similar to PrMC3 [Pinus radiata] GI:5487873 Length = 324 Score = 31.9 bits (69), Expect = 0.42 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +3 Query: 510 HFYWRRRNLMVKPLLKEAGIGGIILENPFYGLRKPIDQ 623 H R + P L + GI GIIL +P++ + PID+ Sbjct: 170 HMAMRAAKEKLSPGLNDTGISGIILLHPYFWSKTPIDE 207 >At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pinus radiata] GI:5487873 Length = 329 Score = 29.5 bits (63), Expect = 2.2 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = +3 Query: 552 LKEAGIGGIILENPFYGLRKPID 620 L E+GI GIIL +P++ + P+D Sbjct: 188 LNESGISGIILVHPYFWSKTPVD 210 >At3g09270.1 68416.m01101 glutathione S-transferase, putative similar to glutathione transferase GB:CAA71784 [Glycine max] Length = 224 Score = 27.9 bits (59), Expect = 6.8 Identities = 17/53 (32%), Positives = 26/53 (49%) Frame = +3 Query: 540 VKPLLKEAGIGGIILENPFYGLRKPIDQIRSSLHNVSDIFVMGGCLILESLVL 698 V+ +LK GI +E YG R P+ + +H + + G I ESLV+ Sbjct: 21 VEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNGRSIAESLVI 73 >At4g32650.2 68417.m04648 inward rectifying potassium channel, putative (KAT3) (AKT4) (KC1) identical to K+ inward rectifying channel protein KC1 [Arabidopsis thaliana] gi|4090537|gb|AAC98810; similar to (KAT1) K+ channel [Arabidopsis thaliana] gi|1165000|emb|CAA63601; Shaker-type channel (1P/6TM), PMID:11500563 Length = 597 Score = 27.5 bits (58), Expect = 9.0 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 3/78 (3%) Frame = -1 Query: 375 SEVSFKKLALGDILFLSNGDGIVSFNQFEAFFTI---RYNFSEPNNLRKFSGLPQPFVKN 205 S V+ + GD+ +++ + +FN F F I Y NL L +++ Sbjct: 283 SIVTLTTVGYGDLHAVNSREK--TFNMFYMLFNIGLTSYIIGIMTNLVVHGALRTFAMRS 340 Query: 204 FVNRILRYTASNLLADIL 151 +N ILRYT+ N L D + Sbjct: 341 AINDILRYTSKNRLPDTM 358 >At4g32650.1 68417.m04647 inward rectifying potassium channel, putative (KAT3) (AKT4) (KC1) identical to K+ inward rectifying channel protein KC1 [Arabidopsis thaliana] gi|4090537|gb|AAC98810; similar to (KAT1) K+ channel [Arabidopsis thaliana] gi|1165000|emb|CAA63601; Shaker-type channel (1P/6TM), PMID:11500563 Length = 662 Score = 27.5 bits (58), Expect = 9.0 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 3/78 (3%) Frame = -1 Query: 375 SEVSFKKLALGDILFLSNGDGIVSFNQFEAFFTI---RYNFSEPNNLRKFSGLPQPFVKN 205 S V+ + GD+ +++ + +FN F F I Y NL L +++ Sbjct: 283 SIVTLTTVGYGDLHAVNSREK--TFNMFYMLFNIGLTSYIIGIMTNLVVHGALRTFAMRS 340 Query: 204 FVNRILRYTASNLLADIL 151 +N ILRYT+ N L D + Sbjct: 341 AINDILRYTSKNRLPDTM 358 >At3g25600.1 68416.m03187 calmodulin, putative similar to calmodulin GI:239841 from [Paramecium tetraurelia] Length = 161 Score = 27.5 bits (58), Expect = 9.0 Identities = 9/12 (75%), Positives = 12/12 (100%) Frame = -1 Query: 327 SNGDGIVSFNQF 292 SNGDG++SFN+F Sbjct: 133 SNGDGVISFNEF 144 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,116,000 Number of Sequences: 28952 Number of extensions: 320760 Number of successful extensions: 871 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 852 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 871 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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