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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20728
         (699 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g12150.1 68416.m01514 expressed protein                             42   3e-04
At3g48690.1 68416.m05317 expressed protein similar to PrMC3 [Pin...    32   0.42 
At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pin...    29   2.2  
At3g09270.1 68416.m01101 glutathione S-transferase, putative sim...    28   6.8  
At4g32650.2 68417.m04648 inward rectifying potassium channel, pu...    27   9.0  
At4g32650.1 68417.m04647 inward rectifying potassium channel, pu...    27   9.0  
At3g25600.1 68416.m03187 calmodulin, putative similar to calmodu...    27   9.0  

>At3g12150.1 68416.m01514 expressed protein
          Length = 363

 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 26/60 (43%), Positives = 36/60 (60%)
 Frame = +3

Query: 510 HFYWRRRNLMVKPLLKEAGIGGIILENPFYGLRKPIDQIRSSLHNVSDIFVMGGCLILES 689
           H Y RR  L   PL+K+  I  ++LE+PFYG R+P  Q  + L  VSD+ ++G   I ES
Sbjct: 134 HTYDRRLRLG-GPLVKQ-NIATMVLESPFYGQRRPFLQCGARLLCVSDLLLLGRATIEES 191



 Score = 29.1 bits (62), Expect = 3.0
 Identities = 17/53 (32%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
 Frame = +2

Query: 179 VYRSILLTKFFTKGWGKP--ENLRRLFGSEKLYRIVKNASNWLKETIPSPLLR 331
           ++R+ +   FF++GWG P  E L R+   ++L+ +     NW     P PL+R
Sbjct: 24  MHRTKMTPPFFSRGWGGPNLELLERMV--QRLFPLEVQGQNW-----PPPLVR 69


>At3g48690.1 68416.m05317 expressed protein similar to PrMC3 [Pinus
           radiata] GI:5487873
          Length = 324

 Score = 31.9 bits (69), Expect = 0.42
 Identities = 14/38 (36%), Positives = 21/38 (55%)
 Frame = +3

Query: 510 HFYWRRRNLMVKPLLKEAGIGGIILENPFYGLRKPIDQ 623
           H   R     + P L + GI GIIL +P++  + PID+
Sbjct: 170 HMAMRAAKEKLSPGLNDTGISGIILLHPYFWSKTPIDE 207


>At3g48700.1 68416.m05318 expressed protein similar to PrMC3 [Pinus
           radiata] GI:5487873
          Length = 329

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 11/23 (47%), Positives = 17/23 (73%)
 Frame = +3

Query: 552 LKEAGIGGIILENPFYGLRKPID 620
           L E+GI GIIL +P++  + P+D
Sbjct: 188 LNESGISGIILVHPYFWSKTPVD 210


>At3g09270.1 68416.m01101 glutathione S-transferase, putative
           similar to glutathione transferase GB:CAA71784 [Glycine
           max]
          Length = 224

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 17/53 (32%), Positives = 26/53 (49%)
 Frame = +3

Query: 540 VKPLLKEAGIGGIILENPFYGLRKPIDQIRSSLHNVSDIFVMGGCLILESLVL 698
           V+ +LK  GI    +E   YG R P+    + +H    + +  G  I ESLV+
Sbjct: 21  VEMVLKLKGIPYEYIEEDVYGNRSPMLLKYNPIHKKVPVLIHNGRSIAESLVI 73


>At4g32650.2 68417.m04648 inward rectifying potassium channel,
           putative (KAT3) (AKT4) (KC1) identical to K+ inward
           rectifying channel protein KC1 [Arabidopsis thaliana]
           gi|4090537|gb|AAC98810; similar to (KAT1) K+ channel
           [Arabidopsis thaliana] gi|1165000|emb|CAA63601;
           Shaker-type channel (1P/6TM), PMID:11500563
          Length = 597

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
 Frame = -1

Query: 375 SEVSFKKLALGDILFLSNGDGIVSFNQFEAFFTI---RYNFSEPNNLRKFSGLPQPFVKN 205
           S V+   +  GD+  +++ +   +FN F   F I    Y      NL     L    +++
Sbjct: 283 SIVTLTTVGYGDLHAVNSREK--TFNMFYMLFNIGLTSYIIGIMTNLVVHGALRTFAMRS 340

Query: 204 FVNRILRYTASNLLADIL 151
            +N ILRYT+ N L D +
Sbjct: 341 AINDILRYTSKNRLPDTM 358


>At4g32650.1 68417.m04647 inward rectifying potassium channel,
           putative (KAT3) (AKT4) (KC1) identical to K+ inward
           rectifying channel protein KC1 [Arabidopsis thaliana]
           gi|4090537|gb|AAC98810; similar to (KAT1) K+ channel
           [Arabidopsis thaliana] gi|1165000|emb|CAA63601;
           Shaker-type channel (1P/6TM), PMID:11500563
          Length = 662

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 3/78 (3%)
 Frame = -1

Query: 375 SEVSFKKLALGDILFLSNGDGIVSFNQFEAFFTI---RYNFSEPNNLRKFSGLPQPFVKN 205
           S V+   +  GD+  +++ +   +FN F   F I    Y      NL     L    +++
Sbjct: 283 SIVTLTTVGYGDLHAVNSREK--TFNMFYMLFNIGLTSYIIGIMTNLVVHGALRTFAMRS 340

Query: 204 FVNRILRYTASNLLADIL 151
            +N ILRYT+ N L D +
Sbjct: 341 AINDILRYTSKNRLPDTM 358


>At3g25600.1 68416.m03187 calmodulin, putative similar to calmodulin
           GI:239841 from [Paramecium tetraurelia]
          Length = 161

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 9/12 (75%), Positives = 12/12 (100%)
 Frame = -1

Query: 327 SNGDGIVSFNQF 292
           SNGDG++SFN+F
Sbjct: 133 SNGDGVISFNEF 144


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,116,000
Number of Sequences: 28952
Number of extensions: 320760
Number of successful extensions: 871
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 871
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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