Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20726
(681 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 35 0.009
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 31 0.15
SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo... 30 0.36
SPAC22E12.14c |sck2||serine/threonine protein kinase Sck2|Schizo... 29 0.47
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.82
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 28 1.1
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb... 27 1.9
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.5
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 27 2.5
SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid phospho... 27 3.3
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 27 3.3
SPAC31A2.10 |||RanGTP-binding protein |Schizosaccharomyces pombe... 26 4.4
SPAC821.12 |orb6||serine/threonine protein kinase Orb6|Schizosac... 26 5.8
SPBC30B4.08 |eri1||double-strand siRNA ribonuclease|Schizosaccha... 26 5.8
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 25 7.7
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1016
Score = 35.1 bits (77), Expect = 0.009
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 25 HVFFAGIDWQQVYHQKYTPPLIP 93
H FF+ I+W +YH++ PP IP
Sbjct: 939 HPFFSNINWDDIYHKRTQPPYIP 961
>SPAC1B9.02c |sck1||serine/threonine protein kinase
Sck1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 696
Score = 31.1 bits (67), Expect = 0.15
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +1
Query: 16 MKEHVFFAGIDWQQVYHQKYTPPLIP 93
+KEH FFA I+W + +K PP P
Sbjct: 557 LKEHPFFADINWDLLSKKKVQPPFKP 582
>SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 569
Score = 29.9 bits (64), Expect = 0.36
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 10 RRMKEHVFFAGIDWQQVYHQKYTPPLIP 93
+ +K H FF IDW+++ +K PP P
Sbjct: 477 QEIKNHPFFDDIDWKKLCAKKIQPPFKP 504
>SPAC22E12.14c |sck2||serine/threonine protein kinase
Sck2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 646
Score = 29.5 bits (63), Expect = 0.47
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +1
Query: 22 EHVFFAGIDWQQVYHQKYTPPLIP 93
+H FF GIDW+++ ++ +PP P
Sbjct: 523 KHPFFDGIDWKKLAAKEISPPFKP 546
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.7 bits (61), Expect = 0.82
Identities = 14/28 (50%), Positives = 17/28 (60%), Gaps = 1/28 (3%)
Frame = +1
Query: 88 IPPRGRSTPPMPSTLEA-STKKTPKELS 168
+PP+GRS PP P A ST + P LS
Sbjct: 353 LPPQGRSAPPPPPPRSAPSTGRQPPPLS 380
Score = 25.4 bits (53), Expect = 7.7
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +1
Query: 79 PPLIPPRGRSTPPMPSTLEASTKKTP 156
PP IP GRS P +P AS TP
Sbjct: 392 PPAIP--GRSAPALPPLGNASRTSTP 415
>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1316
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 16 MKEHVFFAGIDWQQVYHQKYTPPLIP 93
+KEH FF GI+W ++ + P IP
Sbjct: 926 IKEHPFFNGINWDDIF--SHEAPFIP 949
>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 814
Score = 27.5 bits (58), Expect = 1.9
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = +2
Query: 284 EQKRKAKQKQKFDADEKESDCILHGYIKKLG 376
EQK+ KQK K + +EK CIL G +K+G
Sbjct: 301 EQKKAIKQK-KDEEEEKYKWCILDGRKEKVG 330
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 2.5
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +1
Query: 76 TPPLIPPRGRSTPPMPSTLEASTKKTPKELS*QKRIRCNTRTSL 207
T IPP G ST P+ T+ ++ TP + C T TS+
Sbjct: 395 TSTSIPPTGNSTTPVTPTVPPTSSSTPLTTT-----NCTTSTSV 433
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 27.1 bits (57), Expect = 2.5
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +1
Query: 25 HVFFAGIDWQQVYHQKYTPPLIP 93
H FFA I W +Y++ Y P P
Sbjct: 920 HPFFASIVWDDLYNKLYEPSYKP 942
>SPBC32F12.01c ||SPBC685.10c|inositol phosphosphingolipid
phospholipase C |Schizosaccharomyces pombe|chr
2|||Manual
Length = 424
Score = 26.6 bits (56), Expect = 3.3
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -3
Query: 490 LDSVQQNHFGLGAVLEVQLEPVRVQ 416
LD +HF + VL ++L+P+ VQ
Sbjct: 282 LDCSYSDHFAIETVLSIKLQPIPVQ 306
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 26.6 bits (56), Expect = 3.3
Identities = 8/26 (30%), Positives = 16/26 (61%)
Frame = +1
Query: 16 MKEHVFFAGIDWQQVYHQKYTPPLIP 93
+K+H + IDW ++ + PP++P
Sbjct: 344 IKKHRIYRRIDWNKLEKRMLPPPIVP 369
>SPAC31A2.10 |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 460
Score = 26.2 bits (55), Expect = 4.4
Identities = 12/44 (27%), Positives = 22/44 (50%)
Frame = +1
Query: 538 LRTRNDTKVVLTNTDEIGLKEWACRCVRLTNVPRTPCLDGEESR 669
L+ +T + LTN + L E+ R L + + CL+ +S+
Sbjct: 409 LKLEPETSIYLTNPGSLSLLEYLLRLCSLQELHQISCLEMTDSQ 452
>SPAC821.12 |orb6||serine/threonine protein kinase
Orb6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 469
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = +1
Query: 22 EHVFFAGIDWQQVYHQKYTPPLIP 93
+H FF GIDW + ++ P IP
Sbjct: 388 QHPFFTGIDWDHI--RETAAPFIP 409
>SPBC30B4.08 |eri1||double-strand siRNA
ribonuclease|Schizosaccharomyces pombe|chr 2|||Manual
Length = 313
Score = 25.8 bits (54), Expect = 5.8
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +2
Query: 278 KLEQKRKAKQKQKFDADEKESDCI 349
+LE+KRK ++ QKF + C+
Sbjct: 56 RLEEKRKQERYQKFSTSNENKTCL 79
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 25.4 bits (53), Expect = 7.7
Identities = 16/69 (23%), Positives = 30/69 (43%)
Frame = +2
Query: 212 DIGTLAGRSR*DRIHTVNLEADKLEQKRKAKQKQKFDADEKESDCILHGYIKKLGGPFAS 391
D LA + ++ +N + ++LEQ K K D D ++ + + PF +
Sbjct: 852 DDAVLANNEQKLKLTKLNFQVNELEQLEKDINKSSEDCDLQKKKLLEVSSKQGSQAPFLN 911
Query: 392 AWQTRYAKL 418
++ Y KL
Sbjct: 912 ELESEYEKL 920
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,608,581
Number of Sequences: 5004
Number of extensions: 49526
Number of successful extensions: 191
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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