Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20722
(757 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26200.1 68418.m03118 mitochondrial substrate carrier family ... 34 0.12
At3g49990.1 68416.m05466 expressed protein 33 0.27
At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family... 32 0.36
At5g22810.1 68418.m02667 GDSL-motif lipase, putative similar to ... 30 1.9
At3g01290.1 68416.m00037 band 7 family protein similar to hypers... 29 3.3
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 29 4.4
At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putati... 29 4.4
At1g20530.1 68414.m02558 hypothetical protein 29 4.4
At1g06670.1 68414.m00707 DEIH-box RNA/DNA helicase identical to ... 29 4.4
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 28 5.8
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 28 5.8
At1g76710.2 68414.m08928 SET domain-containing protein (ASHH1) l... 28 5.8
At1g76710.1 68414.m08927 SET domain-containing protein (ASHH1) l... 28 5.8
At5g03380.1 68418.m00291 heavy-metal-associated domain-containin... 28 7.7
At3g05900.1 68416.m00664 neurofilament protein-related similar t... 28 7.7
>At5g26200.1 68418.m03118 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 342
Score = 33.9 bits (74), Expect = 0.12
Identities = 18/39 (46%), Positives = 27/39 (69%)
Frame = -1
Query: 232 TSVGLTESSILGATSRIG*AGTTSFSSATGIAKLTAAKA 116
T++ +T+SS+ AT R+G + TTS + A G A LT+A A
Sbjct: 108 TALEITKSSVGQATVRLGLSDTTSLAVANGAAGLTSAVA 146
>At3g49990.1 68416.m05466 expressed protein
Length = 502
Score = 32.7 bits (71), Expect = 0.27
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = +2
Query: 410 IPDAEAKSADIKVEEPAAQPEDSKTEVQATVAEFQKKKNLV 532
+P A+ D+K E P A+P KT Q + E +++KN V
Sbjct: 422 LPGRRAEQTDVKAEIPKAEPIKRKTHGQESKEEKKERKNAV 462
>At5g09530.1 68418.m01103 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 370
Score = 32.3 bits (70), Expect = 0.36
Identities = 24/79 (30%), Positives = 35/79 (44%), Gaps = 2/79 (2%)
Frame = +1
Query: 511 SKEEKPSATDAEGSADSAAIIPNMVK-KIDLAPTVES-DAAAIPEIKTPEAADAPKLADN 684
SK E P + E A +P + K ++ P V +A +PEI+ PE P+L
Sbjct: 173 SKPEVPKLMETE--KPEAPKVPEIPKPELPKLPEVPKLEAPKVPEIQKPELPKMPELPKM 230
Query: 685 PVDEDKPADISPDAPKAEA 741
P + P+ PK EA
Sbjct: 231 PEIQKPELPKLPEVPKLEA 249
>At5g22810.1 68418.m02667 GDSL-motif lipase, putative similar to
EXL3 (GP:15054386) [Arabidopsis thaliana]
Length = 337
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -1
Query: 319 IVLFGLWYSGYLVVTNWSRFGFFSSVAAATSVGLTESSILGATSRIG 179
+V+F ++ Y + T S FGF + A GL E+SIL +G
Sbjct: 254 LVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVG 300
>At3g01290.1 68416.m00037 band 7 family protein similar to
hypersensitive-induced response protein [Zea mays]
GI:7716470; contains Pfam profile PF01145: SPFH domain /
Band 7 family
Length = 285
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -3
Query: 230 FGRFNRVINFGRDF*NWLSGNYV 162
FG+F +V+N G F W+ G+YV
Sbjct: 21 FGKFQKVLNPGLQFVPWVIGDYV 43
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 28.7 bits (61), Expect = 4.4
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +2
Query: 281 DEVPAIPEAKKDDIAPE 331
D+ +IPEAK+DD APE
Sbjct: 197 DDTASIPEAKEDDAAPE 213
>At2g22450.1 68415.m02662 riboflavin biosynthesis protein, putative
similar to SP|P50855 Riboflavin biosynthesis protein
ribA [Includes: GTP cyclohydrolase II (EC 3.5.4.25);
3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP
synthase)] {Actinobacillus pleuropneumoniae}; contains
Pfam profiles PF00925: GTP cyclohydrolase II, PF00926:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Length = 476
Score = 28.7 bits (61), Expect = 4.4
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +1
Query: 487 STSYRR*ISKE--EKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAA 660
S Y R ISK+ + EG S++ I+L P + S E+ P
Sbjct: 29 SLVYPRIISKKFNVRMRVIPEEGDVFSSSKSNGSSMGIELQPDLVSFGTLAAEM-IPTTM 87
Query: 661 DAPKLADNPVDEDKPADISPDAPKA 735
D+P++ D D D+P D P+A
Sbjct: 88 DSPEVEDEEFDLDRPTDGFASIPQA 112
>At1g20530.1 68414.m02558 hypothetical protein
Length = 614
Score = 28.7 bits (61), Expect = 4.4
Identities = 15/54 (27%), Positives = 24/54 (44%)
Frame = +1
Query: 592 IDLAPTVESDAAAIPEIKTPEAADAPKLADNPVDEDKPADISPDAPKAEAKSAD 753
++ P VES+A PE +PE+ + + D D P D + E + D
Sbjct: 60 VESPPDVESEADESPETYSPESCSSAHSVTSS-DSDLPPKFDSDCEEGEGTNCD 112
>At1g06670.1 68414.m00707 DEIH-box RNA/DNA helicase identical to
DEIH-box RNA/DNA helicase GB:BAA84364 GI:5881579
[Arabidopsis thaliana]
Length = 1576
Score = 28.7 bits (61), Expect = 4.4
Identities = 21/89 (23%), Positives = 45/89 (50%), Gaps = 8/89 (8%)
Frame = +1
Query: 514 KEEKPSATDAEGSADSAAIIPNMVK-----KIDLAPTVESDAAAIPEIK---TPEAADAP 669
K+E S + + ++ +I N+V +++AP ++ AA PE K + +
Sbjct: 1306 KKEPKSVSKLDLGSEKVSIPSNLVNGNEQHDLNIAPGEDASAAKQPEKKRSRSKKRKSGN 1365
Query: 670 KLADNPVDEDKPADISPDAPKAEAKSADD 756
L +++ KP+D++ + + E KSA++
Sbjct: 1366 NLDLGKMEKSKPSDLANENEQTEPKSANN 1394
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 5/49 (10%)
Frame = +1
Query: 598 LAPTVESDAAAIPEIKTPEA----ADAPK-LADNPVDEDKPADISPDAP 729
L+P S A +P +P+ AD P+ L+ P D KP+ S +AP
Sbjct: 127 LSPKPPSPRAEVPRSLSPKPPSPRADLPRSLSPKPFDRSKPSSASANAP 175
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 5/49 (10%)
Frame = +1
Query: 598 LAPTVESDAAAIPEIKTPEA----ADAPK-LADNPVDEDKPADISPDAP 729
L+P S A +P +P+ AD P+ L+ P D KP+ S +AP
Sbjct: 126 LSPKPPSPRAEVPRSLSPKPPSPRADLPRSLSPKPFDRSKPSSASANAP 174
>At1g76710.2 68414.m08928 SET domain-containing protein (ASHH1) low
similarity to huntingtin interacting protein 1 [Homo
sapiens] GI:12697196; contains Pfam profile PF00856: SET
domain; identical to cDNA ASH1-like protein 1 (ASHH1)
partial cds GI:15488417
Length = 492
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/65 (24%), Positives = 28/65 (43%)
Frame = +1
Query: 511 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPV 690
+ EEK E +S A+ N+ ++ D PT + +KT + D L+ N
Sbjct: 276 TNEEKEKDISTENHLESTAL--NIQQQSDSTPTPMEEDVVTETVKTETSEDMKLLSQNSQ 333
Query: 691 DEDKP 705
++ P
Sbjct: 334 EDSSP 338
>At1g76710.1 68414.m08927 SET domain-containing protein (ASHH1) low
similarity to huntingtin interacting protein 1 [Homo
sapiens] GI:12697196; contains Pfam profile PF00856: SET
domain; identical to cDNA ASH1-like protein 1 (ASHH1)
partial cds GI:15488417
Length = 492
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/65 (24%), Positives = 28/65 (43%)
Frame = +1
Query: 511 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPV 690
+ EEK E +S A+ N+ ++ D PT + +KT + D L+ N
Sbjct: 276 TNEEKEKDISTENHLESTAL--NIQQQSDSTPTPMEEDVVTETVKTETSEDMKLLSQNSQ 333
Query: 691 DEDKP 705
++ P
Sbjct: 334 EDSSP 338
>At5g03380.1 68418.m00291 heavy-metal-associated domain-containing
protein similar to farnesylated protein ATFP2
[GI:4097545]; contains Pfam profile PF00403:
Heavy-metal-associated domain
Length = 392
Score = 27.9 bits (59), Expect = 7.7
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +1
Query: 547 GSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAADAPKLADNPVDEDKPADISPDA 726
G+ D + + KI P A P+ +TP ++ + +P E+KPA+ P A
Sbjct: 67 GNVDPVEVRDKVADKIK-RPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAA 125
Query: 727 PKAEAKSADD 756
+ + ++
Sbjct: 126 VEKPGEKKEE 135
>At3g05900.1 68416.m00664 neurofilament protein-related similar to
NF-180 (GI:632549) [Petromyzon marinus] similar to
Neurofilament triplet H protein (200 kDa neurofilament
protein) (Neurofilament heavy polypeptide) (NF-H)
(Swiss-Prot:P12036) [Homo sapiens]
Length = 673
Score = 27.9 bits (59), Expect = 7.7
Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 2/71 (2%)
Frame = +1
Query: 511 SKEEKPSATDAEGSADSAAIIPNMVKKIDLAPTVESDAAAIPEIKTPEAA--DAPKLADN 684
SK+EK T GSA + I + +++ + P E++ A I E A + + D
Sbjct: 302 SKDEK---TSESGSALCSEEILSTIQESNTDPIKETEGDASYPIDVIEKAITEEKHVVDE 358
Query: 685 PVDEDKPADIS 717
P +E+KP++ S
Sbjct: 359 PANEEKPSESS 369
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,822,449
Number of Sequences: 28952
Number of extensions: 230135
Number of successful extensions: 832
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 798
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 829
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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