BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20716 (722 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g30080.1 68415.m03660 metal transporter, putative (ZIP6) iden... 40 0.002 At5g62160.1 68418.m07801 metal transporter, putative (ZIP12) ide... 40 0.002 At1g55910.1 68414.m06412 metal transporter, putative (ZIP11) sim... 37 0.016 At1g05300.2 68414.m00537 metal transporter, putative (ZIP5) iden... 34 0.11 At1g05300.1 68414.m00536 metal transporter, putative (ZIP5) iden... 34 0.11 At2g32270.1 68415.m03944 zinc transporter (ZIP3) identical to zi... 33 0.14 At1g31260.1 68414.m03826 metal transporter, putative (ZIP10) ide... 31 0.77 At5g45105.1 68418.m05535 metal transporter, putative (ZIP8) simi... 31 1.0 At4g13550.1 68417.m02112 lipase class 3 family protein very low ... 31 1.0 At5g47110.1 68418.m05808 lil3 protein, putative similar to Lil3 ... 30 1.4 At4g19690.2 68417.m02892 iron-responsive transporter (IRT1) iden... 30 1.4 At4g19690.1 68417.m02891 iron-responsive transporter (IRT1) iden... 30 1.4 At3g12750.1 68416.m01592 zinc transporter (ZIP1) identical to pu... 29 2.4 At2g04032.1 68415.m00373 metal transporter, putative (ZIP7) iden... 29 3.1 At4g19680.1 68417.m02890 iron-responsive transporter (IRT2) memb... 29 4.1 At2g30990.1 68415.m03780 expressed protein contains Pfam profile... 29 4.1 At5g57010.1 68418.m07115 calmodulin-binding family protein conta... 28 5.5 At4g17600.1 68417.m02632 lil3 protein identical to Lil3 protein ... 28 5.5 At1g66680.1 68414.m07579 S locus-linked protein, putative simila... 28 5.5 At5g01590.1 68418.m00074 expressed protein 27 9.5 At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putat... 27 9.5 At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC ... 27 9.5 At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family prote... 27 9.5 At1g60960.1 68414.m06862 metal transporter, putative (IRT3) iden... 27 9.5 >At2g30080.1 68415.m03660 metal transporter, putative (ZIP6) identical to putative metal transporter ZIP6 [Arabidopsis thaliana] gi|17385786|gb|AAL38433; similar to zinc transporter protein ZIP1 [Glycine max] gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 341 Score = 39.9 bits (89), Expect = 0.002 Identities = 18/63 (28%), Positives = 34/63 (53%) Frame = +2 Query: 296 NRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMY 475 ++ ++ + F GV+LST+ +HV+PE E++ + Q + DF L+T G Sbjct: 59 DKAILVIKCFAAGVILSTSLVHVLPEAFESLADCQVSSRHPWKDFPFAGLVTMIGAITAL 118 Query: 476 LIE 484 L++ Sbjct: 119 LVD 121 >At5g62160.1 68418.m07801 metal transporter, putative (ZIP12) identical to putative metal transporter ZIP12 [Arabidopsis thaliana] gi|17385794|gb|AAL38437; similar to zinc transporter protein ZIP1 [Glycine max] gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 355 Score = 39.5 bits (88), Expect = 0.002 Identities = 20/77 (25%), Positives = 38/77 (49%) Frame = +2 Query: 287 KSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFF 466 K+ + M + +F GV+L+T F+H++P+ E++ + DF +T L+ Sbjct: 73 KTESNFFMYVKAFAAGVILATGFVHILPDATESLTSSCLGEEPPWGDFPMTGLVAMAASI 132 Query: 467 IMYLIEELVHIYIHTER 517 + LIE Y++ R Sbjct: 133 LTMLIESFASGYLNRSR 149 >At1g55910.1 68414.m06412 metal transporter, putative (ZIP11) similar to putative zinc transporter ZIP2 [Arabidopsis thaliana] gi|3252868|gb|AAC24198; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 326 Score = 36.7 bits (81), Expect = 0.016 Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 3/67 (4%) Frame = +2 Query: 323 FGGGVLLSTTFMHVMPEVQENIENLQARNILQEFD--FSLTPLLTCCGFFIMYLIEELV- 493 F GGV L+T MH + + E L E + +L C GF + L + ++ Sbjct: 83 FAGGVFLATALMHFLSDADETFRGLLTAEGESEPSPAYPFAYMLACAGFMLTMLADSVIA 142 Query: 494 HIYIHTE 514 HIY T+ Sbjct: 143 HIYSKTQ 149 >At1g05300.2 68414.m00537 metal transporter, putative (ZIP5) identical to putative metal transporter ZIP5 [Arabidopsis thaliana] gi|17385784|gb|AAL38432; similar to zinc transporter protein ZIP1 [Glycine max] gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 267 Score = 33.9 bits (74), Expect = 0.11 Identities = 18/63 (28%), Positives = 29/63 (46%) Frame = +2 Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMYLIEELVHI 499 +F GV+L+T FMHV+PE E + + + EF F T + + ++ Sbjct: 88 AFAAGVILATGFMHVLPEGYEKLTSPCLKGEAWEFPF--TGFIAMVAAILTLSVDSFATS 145 Query: 500 YIH 508 Y H Sbjct: 146 YFH 148 >At1g05300.1 68414.m00536 metal transporter, putative (ZIP5) identical to putative metal transporter ZIP5 [Arabidopsis thaliana] gi|17385784|gb|AAL38432; similar to zinc transporter protein ZIP1 [Glycine max] gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 360 Score = 33.9 bits (74), Expect = 0.11 Identities = 18/63 (28%), Positives = 29/63 (46%) Frame = +2 Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMYLIEELVHI 499 +F GV+L+T FMHV+PE E + + + EF F T + + ++ Sbjct: 88 AFAAGVILATGFMHVLPEGYEKLTSPCLKGEAWEFPF--TGFIAMVAAILTLSVDSFATS 145 Query: 500 YIH 508 Y H Sbjct: 146 YFH 148 >At2g32270.1 68415.m03944 zinc transporter (ZIP3) identical to zinc transporter [Arabidopsis thaliana] gi|3252870|gb|AAC24199; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 339 Score = 33.5 bits (73), Expect = 0.14 Identities = 15/38 (39%), Positives = 23/38 (60%) Frame = +2 Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFS 433 +F GV+L+T FMHV+PE E + + + EF F+ Sbjct: 93 AFAAGVILATGFMHVLPEAYEMLNSPCLTSEAWEFPFT 130 >At1g31260.1 68414.m03826 metal transporter, putative (ZIP10) identical to putative metal transporter ZIP10 [Arabidopsis thaliana] gi|17385792|gb|AAL38436; similar to iron-regulated transporter 2 GB:AAD30549 GI:4836773 from [Lycopersicon esculentum]; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 364 Score = 31.1 bits (67), Expect = 0.77 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = +2 Query: 320 SFGGGVLLSTTFMHVMPEVQENIEN 394 SF G++LST FMHV+P+ E + + Sbjct: 91 SFASGIILSTGFMHVLPDSFEMLSS 115 >At5g45105.1 68418.m05535 metal transporter, putative (ZIP8) similar to putative metal transporter ZIP8 [Arabidopsis thaliana] gi|18997103|gb|AAL83293; similar to iron-regulated transporter 1 [Lycopersicon esculentum] gi|9716481|gb|AAF97509; member to the Zinc (Zn2+)-Iron (Fe2+) Permease (ZIP) family, PMID:11500563 Length = 315 Score = 30.7 bits (66), Expect = 1.0 Identities = 11/32 (34%), Positives = 20/32 (62%) Frame = +2 Query: 299 RVVMTLLSFGGGVLLSTTFMHVMPEVQENIEN 394 ++ M + F G++L T FMHV+P+ E + + Sbjct: 83 KIFMIIKCFASGIILGTGFMHVLPDSFEMLSS 114 >At4g13550.1 68417.m02112 lipase class 3 family protein very low similarity to diacylglycerol lipase [Aspergillus oryzae] GI:1772352; contains Pfam profiles PF01764: Lipase (class 3), PF00168: C2 domain Length = 785 Score = 30.7 bits (66), Expect = 1.0 Identities = 18/64 (28%), Positives = 29/64 (45%) Frame = +1 Query: 430 LTDSPIDVLRLLHNVSDRRIGAHIHPYREKNNGQTDLLVRNLSVRKSRCTEGHSNEAKNS 609 L D+P+ LL+N ++ GA +++ LS K +GH NE ++ Sbjct: 265 LNDAPLKNTELLNNTAEDSEGASSEDSSDQHRSTNLSSSGKLSKDKDGDGDGHGNELEDD 324 Query: 610 TESG 621 ESG Sbjct: 325 NESG 328 >At5g47110.1 68418.m05808 lil3 protein, putative similar to Lil3 protein [Arabidopsis thaliana] gi|4741966|gb|AAD28780 Length = 258 Score = 30.3 bits (65), Expect = 1.4 Identities = 10/31 (32%), Positives = 16/31 (51%) Frame = +1 Query: 109 KQTKWLTDTAMTTMTIQMALYWPKAFPWWCF 201 K+ KWL D TT ++ ++ PWW + Sbjct: 131 KRRKWLEDNPETTSNDELVVFDTSIIPWWAW 161 >At4g19690.2 68417.m02892 iron-responsive transporter (IRT1) identical to Fe(II) transport protein [Arabidopsis thaliana] gi|1353266|gb|AAB01678; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 339 Score = 30.3 bits (65), Expect = 1.4 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%) Frame = +2 Query: 305 VMTLLS-FGGGVLLSTTFMHVMPEVQENIENL-QARNILQEFDFS 433 + T++ F G++L T FMHV+P+ E + ++ N +F FS Sbjct: 77 IFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFS 121 >At4g19690.1 68417.m02891 iron-responsive transporter (IRT1) identical to Fe(II) transport protein [Arabidopsis thaliana] gi|1353266|gb|AAB01678; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 211 Score = 30.3 bits (65), Expect = 1.4 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%) Frame = +2 Query: 305 VMTLLS-FGGGVLLSTTFMHVMPEVQENIENL-QARNILQEFDFS 433 + T++ F G++L T FMHV+P+ E + ++ N +F FS Sbjct: 85 IFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFS 129 >At3g12750.1 68416.m01592 zinc transporter (ZIP1) identical to putative zinc transporter GB:AAC24197 from [Arabidopsis thaliana], ( Proc. Natl. Acad. Sci. U.S.A. 95 (12), 7220-7224 (1998)); member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 355 Score = 29.5 bits (63), Expect = 2.4 Identities = 10/33 (30%), Positives = 20/33 (60%) Frame = +2 Query: 296 NRVVMTLLSFGGGVLLSTTFMHVMPEVQENIEN 394 N + + +F GV+L T F+H++P+ E + + Sbjct: 82 NDIFFMVKAFAAGVILCTGFVHILPDAFERLSS 114 >At2g04032.1 68415.m00373 metal transporter, putative (ZIP7) identical to putative metal transporter ZIP7 [Arabidopsis thaliana] gi|17385788|gb|AAL38434; similar to iron-regulated transporter 1 [Lycopersicon esculentum] gi|9716481|gb|AAF97509; similar to root iron transporter protein [Pisum sativum] gi|3153889|gb|AAC17441; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 365 Score = 29.1 bits (62), Expect = 3.1 Identities = 21/75 (28%), Positives = 39/75 (52%), Gaps = 1/75 (1%) Frame = +2 Query: 263 SVEDAGTFKSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENI-ENLQARNILQEFDFSLT 439 S+ G + + +V TL S GV+L+T FMHV+P+ +++ + Q+F F+ Sbjct: 79 SIPALGPDREMSVIVKTLAS---GVILATGFMHVLPDSFDDLTSKCLPEDPWQKFPFA-- 133 Query: 440 PLLTCCGFFIMYLIE 484 +T ++ +IE Sbjct: 134 TFITMISALLVLMIE 148 >At4g19680.1 68417.m02890 iron-responsive transporter (IRT2) member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 257 Score = 28.7 bits (61), Expect = 4.1 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +2 Query: 308 MTLLSFGGGVLLSTTFMHVMPEVQENIEN 394 M + F G++L T FMHV+P+ E + + Sbjct: 82 MIVKCFSSGIILGTGFMHVLPDSFEMLSS 110 >At2g30990.1 68415.m03780 expressed protein contains Pfam profile: PF05097 protein of unknown function (DUF688) Length = 573 Score = 28.7 bits (61), Expect = 4.1 Identities = 21/67 (31%), Positives = 35/67 (52%), Gaps = 1/67 (1%) Frame = +3 Query: 51 KSTRPLRSPLALLPHRANR-QTNKMAYRHSHDHDDDTDGIVLAKGISMVVLFCASMICGL 227 K +P L+ + N+ + N +RHS D +++ DG IS ++ AS +CGL Sbjct: 217 KPPKPEEPVKQLMKKKQNKVEQNPYRFRHSPDQEEE-DG-----NISSMM---ASGVCGL 267 Query: 228 IPQIIAR 248 +PQ+ R Sbjct: 268 LPQLCLR 274 >At5g57010.1 68418.m07115 calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 495 Score = 28.3 bits (60), Expect = 5.5 Identities = 16/56 (28%), Positives = 26/56 (46%) Frame = +2 Query: 152 RYRWHCIGQRHFHGGAFLCFHDMWSNTSDHREKV*WLSVEDAGTFKSTNRVVMTLL 319 R+ I RH +G ++D+WS + + WL + D G + N+ TLL Sbjct: 213 RHWLEAIDPRHRYGHNLHLYYDVWSESESTQPFFFWLDIGD-GKEVNLNKCSRTLL 267 >At4g17600.1 68417.m02632 lil3 protein identical to Lil3 protein [Arabidopsis thaliana] gi|4741966|gb|AAD28780 Length = 262 Score = 28.3 bits (60), Expect = 5.5 Identities = 10/31 (32%), Positives = 15/31 (48%) Frame = +1 Query: 109 KQTKWLTDTAMTTMTIQMALYWPKAFPWWCF 201 K+ KWL + TT + L+ PWW + Sbjct: 134 KRRKWLEENPETTSNDEPVLFDTSIIPWWAW 164 >At1g66680.1 68414.m07579 S locus-linked protein, putative similar to S locus-linked protein SLL2 [Brassica napus] GI:1518113 Length = 358 Score = 28.3 bits (60), Expect = 5.5 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +1 Query: 544 VRNLSVRKSRCTEGHSNEAKNSTESGIES 630 V N ++RKS G N+A N SG E+ Sbjct: 291 VENFNIRKSNLCRGDGNDANNVLSSGSEA 319 >At5g01590.1 68418.m00074 expressed protein Length = 424 Score = 27.5 bits (58), Expect = 9.5 Identities = 18/61 (29%), Positives = 26/61 (42%) Frame = -3 Query: 390 SMFSWTSGITCMKVVESSTPPPKESRVITTRFVDLNVPASSTESHQTFSR*SEVLDHISW 211 ++F W SG V+ES P S+ TTR N + S + R S D + W Sbjct: 363 ALFLWASGSELTTVLESDHMPTSSSQTTTTRVGQSNTRVKAMGSDK--YRASYGSDQLWW 420 Query: 210 K 208 + Sbjct: 421 R 421 >At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putative (HMA1) contains InterPro accession IPR001757: ATPase, E1-E2 type; identical to Potential cadmium/zinc-transporting ATPase HMA1 (EC 3.6.3.3) (EC 3.6.3.5) (Swiss-Prot:Q9M3H5) [Arabidopsis thaliana]; identical to cDNA putative transcription factor (MYB73) mRNA, partial cds GI:3941503 Length = 819 Score = 27.5 bits (58), Expect = 9.5 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 60 RPLRSPLAL-LPHRANRQTNKMAYRHSHDHDDDTD 161 +PLR +L LP R+ R + H H HDD+ D Sbjct: 44 KPLRISASLNLPPRSIRLRAVEDHHHDHHHDDEQD 78 >At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC family contains Pfam profile: PF05875: alkaline phytoceramidase (aPHC) Length = 255 Score = 27.5 bits (58), Expect = 9.5 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = -2 Query: 541 VDLSVHYSSLCMDVYVHQFFYQIHYEEAAARQ 446 + VHY LC+ + Y IH E+ AA++ Sbjct: 138 IGFKVHYVILCLLCIPRMYKYYIHTEDTAAKR 169 >At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family protein contains Pfam profile PF00561: hydrolase, alpha/beta fold family; contains non-consensus splice site (GC) at intron 10 Length = 565 Score = 27.5 bits (58), Expect = 9.5 Identities = 19/62 (30%), Positives = 24/62 (38%), Gaps = 5/62 (8%) Frame = -3 Query: 285 NVPASSTESHQTFSR*SEVLDHISW----KHRKAPPWKCLWPIQCHLYRHRG-HGCVCKP 121 +VP S T H S S +L HRK +K +WP + R G P Sbjct: 483 SVPISGTHLHNLLSAKSHILLFPGGIREALHRKGEEYKLMWPEKAEFVRAAAKFGAKIVP 542 Query: 120 FC 115 FC Sbjct: 543 FC 544 >At1g60960.1 68414.m06862 metal transporter, putative (IRT3) identical to putative metal transporter IRT3 [Arabidopsis thaliana] gi|17385796|gb|AAL38438; similar to iron-regulated transporter 1 [Lycopersicon esculentum] gi|9716481|gb|AAF97509; member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563 Length = 425 Score = 27.5 bits (58), Expect = 9.5 Identities = 20/73 (27%), Positives = 38/73 (52%) Frame = +2 Query: 287 KSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFF 466 ++ + +T +F GV+L+T F+H++ E ++N L +F +S P GFF Sbjct: 92 QTDGNLFVTAKAFAAGVILATGFVHMLAGGTEALKN----PCLPDFPWSKFPF---PGFF 144 Query: 467 IMYLIEELVHIYI 505 M I L+ +++ Sbjct: 145 AM--IAALITLFV 155 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,870,190 Number of Sequences: 28952 Number of extensions: 340361 Number of successful extensions: 1084 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 1037 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1079 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1575119672 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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