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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20716
         (722 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g30080.1 68415.m03660 metal transporter, putative (ZIP6) iden...    40   0.002
At5g62160.1 68418.m07801 metal transporter, putative (ZIP12) ide...    40   0.002
At1g55910.1 68414.m06412 metal transporter, putative (ZIP11) sim...    37   0.016
At1g05300.2 68414.m00537 metal transporter, putative (ZIP5) iden...    34   0.11 
At1g05300.1 68414.m00536 metal transporter, putative (ZIP5) iden...    34   0.11 
At2g32270.1 68415.m03944 zinc transporter (ZIP3) identical to zi...    33   0.14 
At1g31260.1 68414.m03826 metal transporter, putative (ZIP10) ide...    31   0.77 
At5g45105.1 68418.m05535 metal transporter, putative (ZIP8) simi...    31   1.0  
At4g13550.1 68417.m02112 lipase class 3 family protein very low ...    31   1.0  
At5g47110.1 68418.m05808 lil3 protein, putative similar to Lil3 ...    30   1.4  
At4g19690.2 68417.m02892 iron-responsive transporter (IRT1) iden...    30   1.4  
At4g19690.1 68417.m02891 iron-responsive transporter (IRT1) iden...    30   1.4  
At3g12750.1 68416.m01592 zinc transporter (ZIP1) identical to pu...    29   2.4  
At2g04032.1 68415.m00373 metal transporter, putative (ZIP7) iden...    29   3.1  
At4g19680.1 68417.m02890 iron-responsive transporter (IRT2) memb...    29   4.1  
At2g30990.1 68415.m03780 expressed protein contains Pfam profile...    29   4.1  
At5g57010.1 68418.m07115 calmodulin-binding family protein conta...    28   5.5  
At4g17600.1 68417.m02632 lil3 protein identical to Lil3 protein ...    28   5.5  
At1g66680.1 68414.m07579 S locus-linked protein, putative simila...    28   5.5  
At5g01590.1 68418.m00074 expressed protein                             27   9.5  
At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putat...    27   9.5  
At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC ...    27   9.5  
At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family prote...    27   9.5  
At1g60960.1 68414.m06862 metal transporter, putative (IRT3) iden...    27   9.5  

>At2g30080.1 68415.m03660 metal transporter, putative (ZIP6)
           identical to putative metal transporter ZIP6
           [Arabidopsis thaliana] gi|17385786|gb|AAL38433; similar
           to zinc transporter protein ZIP1 [Glycine max]
           gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 341

 Score = 39.9 bits (89), Expect = 0.002
 Identities = 18/63 (28%), Positives = 34/63 (53%)
 Frame = +2

Query: 296 NRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMY 475
           ++ ++ +  F  GV+LST+ +HV+PE  E++ + Q  +     DF    L+T  G     
Sbjct: 59  DKAILVIKCFAAGVILSTSLVHVLPEAFESLADCQVSSRHPWKDFPFAGLVTMIGAITAL 118

Query: 476 LIE 484
           L++
Sbjct: 119 LVD 121


>At5g62160.1 68418.m07801 metal transporter, putative (ZIP12)
           identical to putative metal transporter ZIP12
           [Arabidopsis thaliana] gi|17385794|gb|AAL38437; similar
           to zinc transporter protein ZIP1 [Glycine max]
           gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 355

 Score = 39.5 bits (88), Expect = 0.002
 Identities = 20/77 (25%), Positives = 38/77 (49%)
 Frame = +2

Query: 287 KSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFF 466
           K+ +   M + +F  GV+L+T F+H++P+  E++ +          DF +T L+      
Sbjct: 73  KTESNFFMYVKAFAAGVILATGFVHILPDATESLTSSCLGEEPPWGDFPMTGLVAMAASI 132

Query: 467 IMYLIEELVHIYIHTER 517
           +  LIE     Y++  R
Sbjct: 133 LTMLIESFASGYLNRSR 149


>At1g55910.1 68414.m06412 metal transporter, putative (ZIP11)
           similar to putative zinc transporter ZIP2 [Arabidopsis
           thaliana] gi|3252868|gb|AAC24198; member of the Zinc
           (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 326

 Score = 36.7 bits (81), Expect = 0.016
 Identities = 20/67 (29%), Positives = 30/67 (44%), Gaps = 3/67 (4%)
 Frame = +2

Query: 323 FGGGVLLSTTFMHVMPEVQENIENLQARNILQEFD--FSLTPLLTCCGFFIMYLIEELV- 493
           F GGV L+T  MH + +  E    L       E    +    +L C GF +  L + ++ 
Sbjct: 83  FAGGVFLATALMHFLSDADETFRGLLTAEGESEPSPAYPFAYMLACAGFMLTMLADSVIA 142

Query: 494 HIYIHTE 514
           HIY  T+
Sbjct: 143 HIYSKTQ 149


>At1g05300.2 68414.m00537 metal transporter, putative (ZIP5)
           identical to putative metal transporter ZIP5
           [Arabidopsis thaliana] gi|17385784|gb|AAL38432; similar
           to zinc transporter protein ZIP1 [Glycine max]
           gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 267

 Score = 33.9 bits (74), Expect = 0.11
 Identities = 18/63 (28%), Positives = 29/63 (46%)
 Frame = +2

Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMYLIEELVHI 499
           +F  GV+L+T FMHV+PE  E + +   +    EF F  T  +      +   ++     
Sbjct: 88  AFAAGVILATGFMHVLPEGYEKLTSPCLKGEAWEFPF--TGFIAMVAAILTLSVDSFATS 145

Query: 500 YIH 508
           Y H
Sbjct: 146 YFH 148


>At1g05300.1 68414.m00536 metal transporter, putative (ZIP5)
           identical to putative metal transporter ZIP5
           [Arabidopsis thaliana] gi|17385784|gb|AAL38432; similar
           to zinc transporter protein ZIP1 [Glycine max]
           gi|15418778|gb|AAK37761; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 360

 Score = 33.9 bits (74), Expect = 0.11
 Identities = 18/63 (28%), Positives = 29/63 (46%)
 Frame = +2

Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFFIMYLIEELVHI 499
           +F  GV+L+T FMHV+PE  E + +   +    EF F  T  +      +   ++     
Sbjct: 88  AFAAGVILATGFMHVLPEGYEKLTSPCLKGEAWEFPF--TGFIAMVAAILTLSVDSFATS 145

Query: 500 YIH 508
           Y H
Sbjct: 146 YFH 148


>At2g32270.1 68415.m03944 zinc transporter (ZIP3) identical to zinc
           transporter [Arabidopsis thaliana]
           gi|3252870|gb|AAC24199; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 339

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 15/38 (39%), Positives = 23/38 (60%)
 Frame = +2

Query: 320 SFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFS 433
           +F  GV+L+T FMHV+PE  E + +    +   EF F+
Sbjct: 93  AFAAGVILATGFMHVLPEAYEMLNSPCLTSEAWEFPFT 130


>At1g31260.1 68414.m03826 metal transporter, putative (ZIP10)
           identical to putative metal transporter ZIP10
           [Arabidopsis thaliana] gi|17385792|gb|AAL38436; similar
           to iron-regulated transporter 2 GB:AAD30549 GI:4836773
           from [Lycopersicon esculentum]; member of the Zinc
           (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 364

 Score = 31.1 bits (67), Expect = 0.77
 Identities = 12/25 (48%), Positives = 18/25 (72%)
 Frame = +2

Query: 320 SFGGGVLLSTTFMHVMPEVQENIEN 394
           SF  G++LST FMHV+P+  E + +
Sbjct: 91  SFASGIILSTGFMHVLPDSFEMLSS 115


>At5g45105.1 68418.m05535 metal transporter, putative (ZIP8) similar
           to putative metal transporter ZIP8 [Arabidopsis
           thaliana] gi|18997103|gb|AAL83293; similar to
           iron-regulated transporter 1 [Lycopersicon esculentum]
           gi|9716481|gb|AAF97509; member to the Zinc (Zn2+)-Iron
           (Fe2+) Permease (ZIP) family, PMID:11500563
          Length = 315

 Score = 30.7 bits (66), Expect = 1.0
 Identities = 11/32 (34%), Positives = 20/32 (62%)
 Frame = +2

Query: 299 RVVMTLLSFGGGVLLSTTFMHVMPEVQENIEN 394
           ++ M +  F  G++L T FMHV+P+  E + +
Sbjct: 83  KIFMIIKCFASGIILGTGFMHVLPDSFEMLSS 114


>At4g13550.1 68417.m02112 lipase class 3 family protein very low
           similarity to diacylglycerol lipase [Aspergillus oryzae]
           GI:1772352; contains Pfam profiles PF01764: Lipase
           (class 3), PF00168: C2 domain
          Length = 785

 Score = 30.7 bits (66), Expect = 1.0
 Identities = 18/64 (28%), Positives = 29/64 (45%)
 Frame = +1

Query: 430 LTDSPIDVLRLLHNVSDRRIGAHIHPYREKNNGQTDLLVRNLSVRKSRCTEGHSNEAKNS 609
           L D+P+    LL+N ++   GA      +++          LS  K    +GH NE ++ 
Sbjct: 265 LNDAPLKNTELLNNTAEDSEGASSEDSSDQHRSTNLSSSGKLSKDKDGDGDGHGNELEDD 324

Query: 610 TESG 621
            ESG
Sbjct: 325 NESG 328


>At5g47110.1 68418.m05808 lil3 protein, putative similar to Lil3
           protein [Arabidopsis thaliana] gi|4741966|gb|AAD28780
          Length = 258

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 10/31 (32%), Positives = 16/31 (51%)
 Frame = +1

Query: 109 KQTKWLTDTAMTTMTIQMALYWPKAFPWWCF 201
           K+ KWL D   TT   ++ ++     PWW +
Sbjct: 131 KRRKWLEDNPETTSNDELVVFDTSIIPWWAW 161


>At4g19690.2 68417.m02892 iron-responsive transporter (IRT1)
           identical to Fe(II) transport protein [Arabidopsis
           thaliana] gi|1353266|gb|AAB01678; member of the Zinc
           (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 339

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
 Frame = +2

Query: 305 VMTLLS-FGGGVLLSTTFMHVMPEVQENIENL-QARNILQEFDFS 433
           + T++  F  G++L T FMHV+P+  E + ++    N   +F FS
Sbjct: 77  IFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFS 121


>At4g19690.1 68417.m02891 iron-responsive transporter (IRT1)
           identical to Fe(II) transport protein [Arabidopsis
           thaliana] gi|1353266|gb|AAB01678; member of the Zinc
           (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 211

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
 Frame = +2

Query: 305 VMTLLS-FGGGVLLSTTFMHVMPEVQENIENL-QARNILQEFDFS 433
           + T++  F  G++L T FMHV+P+  E + ++    N   +F FS
Sbjct: 85  IFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFS 129


>At3g12750.1 68416.m01592 zinc transporter (ZIP1) identical to
           putative zinc transporter GB:AAC24197 from [Arabidopsis
           thaliana], ( Proc. Natl. Acad. Sci. U.S.A. 95 (12),
           7220-7224 (1998)); member of the Zinc (Zn2+)-Iron (Fe2+)
           permease (ZIP) family, PMID:11500563
          Length = 355

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 10/33 (30%), Positives = 20/33 (60%)
 Frame = +2

Query: 296 NRVVMTLLSFGGGVLLSTTFMHVMPEVQENIEN 394
           N +   + +F  GV+L T F+H++P+  E + +
Sbjct: 82  NDIFFMVKAFAAGVILCTGFVHILPDAFERLSS 114


>At2g04032.1 68415.m00373 metal transporter, putative (ZIP7)
           identical to putative metal transporter ZIP7
           [Arabidopsis thaliana] gi|17385788|gb|AAL38434; similar
           to iron-regulated transporter 1 [Lycopersicon
           esculentum] gi|9716481|gb|AAF97509; similar to root iron
           transporter protein [Pisum sativum]
           gi|3153889|gb|AAC17441; member of the Zinc (Zn2+)-Iron
           (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 365

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 21/75 (28%), Positives = 39/75 (52%), Gaps = 1/75 (1%)
 Frame = +2

Query: 263 SVEDAGTFKSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENI-ENLQARNILQEFDFSLT 439
           S+   G  +  + +V TL S   GV+L+T FMHV+P+  +++       +  Q+F F+  
Sbjct: 79  SIPALGPDREMSVIVKTLAS---GVILATGFMHVLPDSFDDLTSKCLPEDPWQKFPFA-- 133

Query: 440 PLLTCCGFFIMYLIE 484
             +T     ++ +IE
Sbjct: 134 TFITMISALLVLMIE 148


>At4g19680.1 68417.m02890 iron-responsive transporter (IRT2) member
           of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family,
           PMID:11500563
          Length = 257

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 308 MTLLSFGGGVLLSTTFMHVMPEVQENIEN 394
           M +  F  G++L T FMHV+P+  E + +
Sbjct: 82  MIVKCFSSGIILGTGFMHVLPDSFEMLSS 110


>At2g30990.1 68415.m03780 expressed protein contains Pfam profile:
           PF05097 protein of unknown function (DUF688)
          Length = 573

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 21/67 (31%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
 Frame = +3

Query: 51  KSTRPLRSPLALLPHRANR-QTNKMAYRHSHDHDDDTDGIVLAKGISMVVLFCASMICGL 227
           K  +P      L+  + N+ + N   +RHS D +++ DG      IS ++   AS +CGL
Sbjct: 217 KPPKPEEPVKQLMKKKQNKVEQNPYRFRHSPDQEEE-DG-----NISSMM---ASGVCGL 267

Query: 228 IPQIIAR 248
           +PQ+  R
Sbjct: 268 LPQLCLR 274


>At5g57010.1 68418.m07115 calmodulin-binding family protein contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 495

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 16/56 (28%), Positives = 26/56 (46%)
 Frame = +2

Query: 152 RYRWHCIGQRHFHGGAFLCFHDMWSNTSDHREKV*WLSVEDAGTFKSTNRVVMTLL 319
           R+    I  RH +G     ++D+WS +   +    WL + D G   + N+   TLL
Sbjct: 213 RHWLEAIDPRHRYGHNLHLYYDVWSESESTQPFFFWLDIGD-GKEVNLNKCSRTLL 267


>At4g17600.1 68417.m02632 lil3 protein identical to Lil3 protein
           [Arabidopsis thaliana] gi|4741966|gb|AAD28780
          Length = 262

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 10/31 (32%), Positives = 15/31 (48%)
 Frame = +1

Query: 109 KQTKWLTDTAMTTMTIQMALYWPKAFPWWCF 201
           K+ KWL +   TT   +  L+     PWW +
Sbjct: 134 KRRKWLEENPETTSNDEPVLFDTSIIPWWAW 164


>At1g66680.1 68414.m07579 S locus-linked protein, putative similar
           to S locus-linked protein SLL2 [Brassica napus]
           GI:1518113
          Length = 358

 Score = 28.3 bits (60), Expect = 5.5
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +1

Query: 544 VRNLSVRKSRCTEGHSNEAKNSTESGIES 630
           V N ++RKS    G  N+A N   SG E+
Sbjct: 291 VENFNIRKSNLCRGDGNDANNVLSSGSEA 319


>At5g01590.1 68418.m00074 expressed protein
          Length = 424

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 18/61 (29%), Positives = 26/61 (42%)
 Frame = -3

Query: 390 SMFSWTSGITCMKVVESSTPPPKESRVITTRFVDLNVPASSTESHQTFSR*SEVLDHISW 211
           ++F W SG     V+ES   P   S+  TTR    N    +  S +   R S   D + W
Sbjct: 363 ALFLWASGSELTTVLESDHMPTSSSQTTTTRVGQSNTRVKAMGSDK--YRASYGSDQLWW 420

Query: 210 K 208
           +
Sbjct: 421 R 421


>At4g37270.1 68417.m05275 cadmium/zinc-transporting ATPase, putative
           (HMA1) contains InterPro accession IPR001757: ATPase,
           E1-E2 type; identical to Potential
           cadmium/zinc-transporting ATPase HMA1 (EC 3.6.3.3) (EC
           3.6.3.5) (Swiss-Prot:Q9M3H5) [Arabidopsis thaliana];
           identical to cDNA putative transcription factor (MYB73)
           mRNA, partial cds GI:3941503
          Length = 819

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 60  RPLRSPLAL-LPHRANRQTNKMAYRHSHDHDDDTD 161
           +PLR   +L LP R+ R      + H H HDD+ D
Sbjct: 44  KPLRISASLNLPPRSIRLRAVEDHHHDHHHDDEQD 78


>At4g22330.1 68417.m03228 alkaline phytoceramidase family / aPHC
           family contains Pfam profile: PF05875: alkaline
           phytoceramidase (aPHC)
          Length = 255

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = -2

Query: 541 VDLSVHYSSLCMDVYVHQFFYQIHYEEAAARQ 446
           +   VHY  LC+      + Y IH E+ AA++
Sbjct: 138 IGFKVHYVILCLLCIPRMYKYYIHTEDTAAKR 169


>At3g02030.1 68416.m00166 hydrolase, alpha/beta fold family protein
           contains Pfam profile PF00561: hydrolase, alpha/beta
           fold family; contains non-consensus splice site (GC) at
           intron 10
          Length = 565

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 19/62 (30%), Positives = 24/62 (38%), Gaps = 5/62 (8%)
 Frame = -3

Query: 285 NVPASSTESHQTFSR*SEVLDHISW----KHRKAPPWKCLWPIQCHLYRHRG-HGCVCKP 121
           +VP S T  H   S  S +L          HRK   +K +WP +    R     G    P
Sbjct: 483 SVPISGTHLHNLLSAKSHILLFPGGIREALHRKGEEYKLMWPEKAEFVRAAAKFGAKIVP 542

Query: 120 FC 115
           FC
Sbjct: 543 FC 544


>At1g60960.1 68414.m06862 metal transporter, putative (IRT3)
           identical to putative metal transporter IRT3
           [Arabidopsis thaliana] gi|17385796|gb|AAL38438; similar
           to iron-regulated transporter 1 [Lycopersicon
           esculentum] gi|9716481|gb|AAF97509; member of the Zinc
           (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
          Length = 425

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 20/73 (27%), Positives = 38/73 (52%)
 Frame = +2

Query: 287 KSTNRVVMTLLSFGGGVLLSTTFMHVMPEVQENIENLQARNILQEFDFSLTPLLTCCGFF 466
           ++   + +T  +F  GV+L+T F+H++    E ++N      L +F +S  P     GFF
Sbjct: 92  QTDGNLFVTAKAFAAGVILATGFVHMLAGGTEALKN----PCLPDFPWSKFPF---PGFF 144

Query: 467 IMYLIEELVHIYI 505
            M  I  L+ +++
Sbjct: 145 AM--IAALITLFV 155


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,870,190
Number of Sequences: 28952
Number of extensions: 340361
Number of successful extensions: 1084
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1037
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1079
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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