Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20706
         (620 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...   110   1e-25
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    57   2e-09
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar...    34   0.014
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    28   1.3  
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    27   1.7  
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar...    26   3.8  
SPCC1235.04c |||FAD synthetase|Schizosaccharomyces pombe|chr 3||...    26   3.8  
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc...    26   5.1  
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos...    25   6.7  

>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score =  110 bits (265), Expect = 1e-25
 Identities = 49/85 (57%), Positives = 64/85 (75%)
 Frame = +1

Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
           GADTRAT   +++DKNC+K+H ++ N++C GAGTAADTE  T  ++S +EL  L+T R  
Sbjct: 50  GADTRATAGPIIADKNCKKLHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKP 109

Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
            V TA T+LK+ LFRYQGHIGA LV
Sbjct: 110 RVVTALTMLKQHLFRYQGHIGAYLV 134



 Score = 59.3 bits (137), Expect = 4e-10
 Identities = 25/40 (62%), Positives = 30/40 (75%)
 Frame = +2

Query: 134 GFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVI 253
           GF FE +QRN  L +KGFP PKAT TGTTIVG+I  D ++
Sbjct: 9   GFDFEYYQRNLLLQEKGFPTPKATSTGTTIVGVIAKDCIV 48



 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 23/37 (62%), Positives = 28/37 (75%)
 Frame = +3

Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMA 620
           LGG D  GPH++ I  HGS DKLPY  +GSGSLAA++
Sbjct: 135 LGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAIS 171


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 57.2 bits (132), Expect = 2e-09
 Identities = 26/84 (30%), Positives = 45/84 (53%)
 Frame = +1

Query: 259 ADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVP 438
           AD+R T    ++++   K+  L  N++CC +G+AADT+     +   L + R+  G    
Sbjct: 40  ADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLLKYYLSMYRIQFGHDPS 99

Query: 439 VETAATLLKRMLFRYQGHIGAALV 510
           V TAATL   M ++ +  + A L+
Sbjct: 100 VHTAATLASEMCYQNKNMLSAGLI 123



 Score = 27.5 bits (58), Expect = 1.7
 Identities = 21/56 (37%), Positives = 26/56 (46%), Gaps = 5/56 (8%)
 Frame = +3

Query: 453 HTAETNAVSLSRSYRGCSGLG----GVD-RTGPHIYCIYPHGSVDKLPYATMGSGS 605
           HTA T A  +    +     G    G D +TG  +Y I   GS+ K P A  GSGS
Sbjct: 101 HTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLGGSLHKQPLAIGGSGS 156


>SPAC4A8.13c |pts1||20S proteasome component beta
           5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 34.3 bits (75), Expect = 0.014
 Identities = 19/79 (24%), Positives = 39/79 (49%), Gaps = 2/79 (2%)
 Frame = +1

Query: 262 DTRATENTVVSDKNCQKIHYLASNMYCCG--AGTAADTEMTTQSVASQLELQRLHTGRTV 435
           D+RA+   +++ +  +K+  +  N Y  G  AG AAD +     +  +  L +L     +
Sbjct: 78  DSRASAGPLIASQTVKKV--IEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELI 135

Query: 436 PVETAATLLKRMLFRYQGH 492
            V  A+ +L  + + Y+G+
Sbjct: 136 SVSAASKILSNITYSYKGY 154


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 13/42 (30%), Positives = 21/42 (50%)
 Frame = +3

Query: 480 LSRSYRGCSGLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGS 605
           +SR +     + G+D  GP +Y   P G+  +     +GSGS
Sbjct: 134 MSRPFGVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGS 175


>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 27.5 bits (58), Expect = 1.7
 Identities = 17/46 (36%), Positives = 21/46 (45%)
 Frame = +2

Query: 137 FSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVIQELTQEP 274
           FS   F  N  L Q  + A  A   G T VGI   DGV+    ++P
Sbjct: 7   FSLTTFSPNGKLVQIEY-ALNAVNAGVTSVGIKATDGVVLATEKKP 51



 Score = 25.0 bits (52), Expect = 8.8
 Identities = 11/35 (31%), Positives = 17/35 (48%)
 Frame = +3

Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAA 614
           + G+D  GP +Y + P G+        +G  S AA
Sbjct: 136 VAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAA 170


>SPAC22F8.06 |pam1||20S proteasome component beta
           6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 225

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 11/72 (15%), Positives = 35/72 (48%)
 Frame = +1

Query: 262 DTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVPV 441
           DTR+     ++ +   ++H +  ++    +G  AD     + +  +++L   +  R +  
Sbjct: 30  DTRSVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVKRIQQRIDLYHDNHERKMSA 89

Query: 442 ETAATLLKRMLF 477
           ++ A +++ +L+
Sbjct: 90  QSCACMVRTLLY 101


>SPCC1235.04c |||FAD synthetase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 265

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 18/49 (36%), Positives = 22/49 (44%)
 Frame = +3

Query: 462 ETNAVSLSRSYRGCSGLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSL 608
           ETN    S   RG + LGGV  T P+     P G+    P   +  GSL
Sbjct: 186 ETNTKYCSLYDRGYTSLGGVSDTSPNPALKNPDGTYS--PAYLLSDGSL 232


>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
           Pre5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = +3

Query: 516 GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAA 614
           G D +GPH+    P G V +    +MGS S +A
Sbjct: 136 GYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSA 168


>SPBP35G2.05c |cki2||serine/threonine protein kinase
           Cki2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 435

 Score = 25.4 bits (53), Expect = 6.7
 Identities = 12/31 (38%), Positives = 15/31 (48%)
 Frame = -3

Query: 573 YLQIHEDKCSKYEDRFCQHLPNQSSPYMTLI 481
           Y +I E K S      C   PN+ S YMT +
Sbjct: 230 YEKISEKKQSTSISELCAGFPNEFSKYMTYV 260


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,641,697
Number of Sequences: 5004
Number of extensions: 55781
Number of successful extensions: 152
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)