Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20706
(620 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 126 2e-29
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 126 2e-29
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 125 2e-29
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 125 2e-29
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 48 5e-06
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 41 8e-04
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 41 8e-04
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 33 0.20
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 30 1.1
At5g04020.1 68418.m00382 calmodulin-binding protein-related (PIC... 30 1.1
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 30 1.1
At5g23575.1 68418.m02766 transmembrane protein, putative similar... 27 7.6
At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein con... 27 7.6
At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1) 27 7.6
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 126 bits (303), Expect = 2e-29
Identities = 58/85 (68%), Positives = 66/85 (77%)
Frame = +1
Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
GADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 54 GADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDS 113
Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
V TA TLLK+ LF YQGH+ AALV
Sbjct: 114 RVVTALTLLKKHLFSYQGHVSAALV 138
Score = 68.1 bits (159), Expect = 4e-12
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = +3
Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMA 620
LGGVD TGPH++ IYPHGS D LP+ATMGSGSLAAM+
Sbjct: 139 LGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMS 175
Score = 56.0 bits (129), Expect = 2e-08
Identities = 28/52 (53%), Positives = 34/52 (65%)
Frame = +2
Query: 98 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVI 253
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVI
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVI 52
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 126 bits (303), Expect = 2e-29
Identities = 58/85 (68%), Positives = 66/85 (77%)
Frame = +1
Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
GADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 54 GADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDS 113
Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
V TA TLLK+ LF YQGH+ AALV
Sbjct: 114 RVVTALTLLKKHLFSYQGHVSAALV 138
Score = 68.1 bits (159), Expect = 4e-12
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = +3
Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMA 620
LGGVD TGPH++ IYPHGS D LP+ATMGSGSLAAM+
Sbjct: 139 LGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMS 175
Score = 56.0 bits (129), Expect = 2e-08
Identities = 28/52 (53%), Positives = 34/52 (65%)
Frame = +2
Query: 98 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVI 253
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVI
Sbjct: 1 MSQSSVDIPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVI 52
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 125 bits (302), Expect = 2e-29
Identities = 58/85 (68%), Positives = 66/85 (77%)
Frame = +1
Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
GADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 54 GADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDS 113
Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
V TA TLLK+ LF YQGH+ AALV
Sbjct: 114 RVITALTLLKKHLFSYQGHVSAALV 138
Score = 68.1 bits (159), Expect = 4e-12
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = +3
Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMA 620
LGGVD TGPH++ IYPHGS D LP+ATMGSGSLAAM+
Sbjct: 139 LGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMS 175
Score = 56.8 bits (131), Expect = 1e-08
Identities = 28/52 (53%), Positives = 34/52 (65%)
Frame = +2
Query: 98 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVI 253
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVI
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVI 52
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 125 bits (302), Expect = 2e-29
Identities = 58/85 (68%), Positives = 66/85 (77%)
Frame = +1
Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
GADTRATE +V+DKNC+KIHY+A N+YCCGAGTAADTE T V+SQL L R TGR
Sbjct: 54 GADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMVSSQLRLHRYQTGRDS 113
Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
V TA TLLK+ LF YQGH+ AALV
Sbjct: 114 RVITALTLLKKHLFSYQGHVSAALV 138
Score = 68.1 bits (159), Expect = 4e-12
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = +3
Query: 510 LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMA 620
LGGVD TGPH++ IYPHGS D LP+ATMGSGSLAAM+
Sbjct: 139 LGGVDITGPHLHTIYPHGSTDTLPFATMGSGSLAAMS 175
Score = 56.8 bits (131), Expect = 1e-08
Identities = 28/52 (53%), Positives = 34/52 (65%)
Frame = +2
Query: 98 MASALVPEIPVPGFSFENFQRNAFLAQKGFPAPKATKTGTTIVGIIYADGVI 253
M+ + V P GFSF+ +RN L QKG AP KTGTTIVG+I+ DGVI
Sbjct: 1 MSQSTVDVPPKGGFSFDLCKRNDMLTQKGLKAPSFLKTGTTIVGLIFKDGVI 52
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 48.0 bits (109), Expect = 5e-06
Identities = 22/85 (25%), Positives = 45/85 (52%)
Frame = +1
Query: 256 GADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTV 435
GAD+R + V+++ KI L N+Y C +G+AAD+++ + V L + G+
Sbjct: 27 GADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRYFLHQHTIQHGQPA 86
Query: 436 PVETAATLLKRMLFRYQGHIGAALV 510
V+ +A L++ + + + + L+
Sbjct: 87 TVKVSANLIRMLAYNNKNMLQTGLI 111
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 40.7 bits (91), Expect = 8e-04
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +1
Query: 259 ADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVP 438
AD+RA+ +S ++ +KI + M AG AAD + +++ + L L R +
Sbjct: 73 ADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRIS 132
Query: 439 VETAATLLKRMLFRYQG 489
V A+ LL ML+ Y+G
Sbjct: 133 VSGASKLLANMLYSYRG 149
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 40.7 bits (91), Expect = 8e-04
Identities = 24/77 (31%), Positives = 40/77 (51%)
Frame = +1
Query: 259 ADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVP 438
AD+RA+ +S ++ +KI + M AG AAD + +++ + L L R +
Sbjct: 73 ADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRIS 132
Query: 439 VETAATLLKRMLFRYQG 489
V A+ LL ML+ Y+G
Sbjct: 133 VSGASKLLANMLYSYRG 149
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 32.7 bits (71), Expect = 0.20
Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +3
Query: 498 GCSG-LGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAA 614
GC LGG DR GP +Y I P G + A +G G AA
Sbjct: 133 GCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGKQAA 172
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 4/58 (6%)
Frame = +3
Query: 453 HTAETNAVSLSRSYRGCSGLG----GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAA 614
H A+ V RS++ G+G G+D +G H+Y P G+ + +GS S AA
Sbjct: 111 HLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 168
>At5g04020.1 68418.m00382 calmodulin-binding protein-related (PICBP)
contains similarity to potato calmodulin-binding protein
PCBP GI:17933110 from [Solanum tuberosum]
Length = 1495
Score = 30.3 bits (65), Expect = 1.1
Identities = 24/92 (26%), Positives = 41/92 (44%), Gaps = 4/92 (4%)
Frame = +1
Query: 247 RYTGADTRATENTVVSDKNCQKIHYLASNMYCCGAGT----AADTEMTTQSVASQLELQR 414
RY G +AT ++ + + ++ + + + YC G AAD S+ + ++R
Sbjct: 204 RYNGL-LKATCSSAMKGSSSKRSNDVCTYRYCSLHGRRHSHAADNNAGVPSLKRFVSMRR 262
Query: 415 LHTGRTVPVETAATLLKRMLFRYQGHIGAALV 510
R V LLKR L R +G +G +V
Sbjct: 263 KFMNRQKSVNRRLVLLKRTLSRKRGPLGGRVV 294
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 4/58 (6%)
Frame = +3
Query: 453 HTAETNAVSLSRSYRGCSGLG----GVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAA 614
H A+ V RS++ G+G G+D +G H+Y P G+ + +GS S AA
Sbjct: 111 HLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 168
>At5g23575.1 68418.m02766 transmembrane protein, putative similar to
cleft lip and palate transmembrane protein 1 [Homo
sapiens] GI:4039014; contains Pfam profile PF05602:
Cleft lip and palate transmembrane protein 1 (CLPTM1)
Length = 593
Score = 27.5 bits (58), Expect = 7.6
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = -2
Query: 391 QQIVLSFQCQL---LFLLHNNTCWML 323
+ +VL+F CQ L+LL N+T WM+
Sbjct: 368 KSVVLNFICQFVIFLYLLDNDTSWMI 393
>At5g08500.1 68418.m01007 transmembrane CLPTM1 family protein
contains Pfam profile PF05602: Cleft lip and palate
transmembrane protein 1 (CLPTM1)
Length = 590
Score = 27.5 bits (58), Expect = 7.6
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = -2
Query: 391 QQIVLSFQCQL---LFLLHNNTCWML 323
+ +VL+F CQ L+LL N+T WM+
Sbjct: 366 KSVVLNFICQFIIFLYLLDNDTSWMI 391
>At3g60820.1 68416.m06804 20S proteasome beta subunit F1 (PBF1)
Length = 223
Score = 27.5 bits (58), Expect = 7.6
Identities = 17/73 (23%), Positives = 31/73 (42%)
Frame = +1
Query: 259 ADTRATENTVVSDKNCQKIHYLASNMYCCGAGTAADTEMTTQSVASQLELQRLHTGRTVP 438
ADTR + + ++ KIH LA +G AD + + + S+ + + + +
Sbjct: 31 ADTRMSTGYSILSRDYSKIHKLADRAVLSSSGFQADVKALQKVLKSRHLIYQHQHNKQMS 90
Query: 439 VETAATLLKRMLF 477
A LL L+
Sbjct: 91 CPAMAQLLSNTLY 103
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,818,964
Number of Sequences: 28952
Number of extensions: 295903
Number of successful extensions: 805
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1255974912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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