BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20688 (711 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 154 1e-38 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 100 2e-22 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 100 4e-22 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 79 5e-16 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 29 0.87 SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 27 2.6 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.5 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 27 3.5 SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex ... 26 4.6 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 25 8.1 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 154 bits (374), Expect = 1e-38 Identities = 67/83 (80%), Positives = 75/83 (90%) Frame = +3 Query: 261 SGGKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 440 +GGKYVPRA+L+DLEPGTMDAV+SG +G LFRPDN ++GQSGAGN WAKGHYTEGAEL D Sbjct: 55 AGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELAD 114 Query: 441 AVLDVVRKECENCDCLQGFQLTH 509 AVLDVVR+E E CD LQGFQLTH Sbjct: 115 AVLDVVRREAEACDALQGFQLTH 137 Score = 91.5 bits (217), Expect = 1e-19 Identities = 36/55 (65%), Positives = 47/55 (85%) Frame = +1 Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEAS 246 MREIVH+QAGQCGNQ+GA FW I++EHG+D G+Y GTS+ Q ER++VY+NEA+ Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAA 55 Score = 84.2 bits (199), Expect = 2e-17 Identities = 36/46 (78%), Positives = 42/46 (91%) Frame = +1 Query: 574 PDRIMNTYSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 711 PDR+M T+SV P+PK SDTVVEPYNA LS+HQLVEN+DET+CIDNE Sbjct: 160 PDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNE 205 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 100 bits (240), Expect = 2e-22 Identities = 45/86 (52%), Positives = 60/86 (69%), Gaps = 1/86 (1%) Frame = +3 Query: 255 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAE 431 +E+G GKYVPR+I +DLEP +D VR+G Y LF P+ + G+ A NN+A+GHYT G E Sbjct: 54 SETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKE 113 Query: 432 LVDAVLDVVRKECENCDCLQGFQLTH 509 LVD V D +R+ +NC LQGF + H Sbjct: 114 LVDEVTDKIRRIADNCSGLQGFLVFH 139 Score = 48.8 bits (111), Expect = 8e-07 Identities = 20/39 (51%), Positives = 30/39 (76%) Frame = +1 Query: 595 YSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 711 +SV P+P+VS +VVEPYN+VL+ H ++ D T+ +DNE Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNE 207 Score = 44.0 bits (99), Expect = 2e-05 Identities = 18/34 (52%), Positives = 21/34 (61%) Frame = +1 Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTG 183 MREI+ + GQ G QIG WE+ EHGI P G Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNG 34 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 99.5 bits (237), Expect = 4e-22 Identities = 43/86 (50%), Positives = 62/86 (72%), Gaps = 1/86 (1%) Frame = +3 Query: 255 AESG-GKYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQSGAGNNWAKGHYTEGAE 431 +E+G GK+VPR+I +DLEP +D VR+G Y LF P+ V G+ A NN+A+GHYT G E Sbjct: 58 SETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKE 117 Query: 432 LVDAVLDVVRKECENCDCLQGFQLTH 509 ++D+VL+ +R+ +NC LQGF + H Sbjct: 118 MIDSVLERIRRMADNCSGLQGFLVFH 143 Score = 52.8 bits (121), Expect = 5e-08 Identities = 21/39 (53%), Positives = 32/39 (82%) Frame = +1 Query: 595 YSVVPSPKVSDTVVEPYNAVLSIHQLVENTDETYCIDNE 711 +SV P+P+VS +VVEPYN+VL+ H ++N+D T+ +DNE Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNE 211 Score = 42.3 bits (95), Expect = 7e-05 Identities = 20/53 (37%), Positives = 30/53 (56%) Frame = +1 Query: 82 MREIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNE 240 MRE++ + GQ G QIG WE+ EHGI P G T + ++ + + Y N+ Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGF--PTENSEVHKNNSYLND 51 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 79.4 bits (187), Expect = 5e-16 Identities = 34/85 (40%), Positives = 55/85 (64%), Gaps = 2/85 (2%) Frame = +3 Query: 270 KYVPRAILLDLEPGTMDAVRSGAYGQLFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDA 443 +Y+PRAIL+DLEP ++ + S YG L+ P+N + ++ GAGNNWA G Y+ + + Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFED 117 Query: 444 VLDVVRKECENCDCLQGFQLTHPSA 518 ++D++ +E + D L+GF L H A Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIA 142 Score = 57.6 bits (133), Expect = 2e-09 Identities = 24/53 (45%), Positives = 36/53 (67%) Frame = +1 Query: 85 REIVHLQAGQCGNQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERISVYYNEA 243 REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R V++ ++ Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQS 55 Score = 49.6 bits (113), Expect = 4e-07 Identities = 23/48 (47%), Positives = 32/48 (66%), Gaps = 1/48 (2%) Frame = +1 Query: 568 RVPDRIMNTYSVVP-SPKVSDTVVEPYNAVLSIHQLVENTDETYCIDN 708 R P +I+ TYSV P S VSD VV+PYN++L++ +L N D +DN Sbjct: 160 RYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDN 207 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 28.7 bits (61), Expect = 0.87 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = +3 Query: 402 AKGHYTEGAELVDAVLDVVRKECENCDCLQ 491 A+GH G ELV A D +RK+ EN L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1131 Score = 27.1 bits (57), Expect = 2.6 Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 3/72 (4%) Frame = +3 Query: 477 CDCLQGFQLTHPSAAAPVP---AWATSSSQRSAKSTRQNHEHILSSPLAQSIRHCRRTIQ 647 C C+ GFQ + V A+SSS SA +T I +SP+ S + +I Sbjct: 317 CFCVVGFQQGTTAKIVVVDYSEEMASSSSSASATATSSAESSIATSPITSS-SNVVSSIS 375 Query: 648 RSSLHPSTSREY 683 SS+ S Y Sbjct: 376 TSSMDSSAVSSY 387 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 26.6 bits (56), Expect = 3.5 Identities = 10/34 (29%), Positives = 21/34 (61%) Frame = +1 Query: 121 NQIGAKFWEIISEEHGIDPTGVYRGTSDLQLERI 222 N++G E+++E+ +DPT + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/20 (55%), Positives = 12/20 (60%) Frame = -1 Query: 483 NRSSHTPCGLHQGRRRRAQR 424 +R H P G H GR RRA R Sbjct: 50 SRMDHRPDGYHDGRGRRAYR 69 >SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex subunit Sld3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 668 Score = 26.2 bits (55), Expect = 4.6 Identities = 9/18 (50%), Positives = 11/18 (61%) Frame = +1 Query: 505 LIPRRRHRFRHGQPPHLK 558 L+P R H+ RH PP K Sbjct: 425 LLPERSHKHRHSAPPRSK 442 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 25.4 bits (53), Expect = 8.1 Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%) Frame = +3 Query: 333 GAYGQLFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 434 G YG +F P F+F +G NW+ Y E A L Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,370,860 Number of Sequences: 5004 Number of extensions: 42081 Number of successful extensions: 158 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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