Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20679
(783 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 126 1e-29
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 125 4e-29
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 125 4e-29
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 124 9e-29
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 120 8e-28
At5g42250.1 68418.m05143 alcohol dehydrogenase, putative similar... 28 6.1
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 126 bits (305), Expect = 1e-29
Identities = 72/175 (41%), Positives = 100/175 (57%), Gaps = 2/175 (1%)
Frame = +2
Query: 257 MVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHE 436
MV++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH
Sbjct: 50 MVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHG 109
Query: 437 N--RNDEEVGAGDQGLMFGYATDETENACR*L*CLHTNSIRKLQSSGEMENFGGQDQIQK 610
+ + EEVGAGDQG MFGYATDET L T KL + K
Sbjct: 110 HLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGK 169
Query: 611 HRLLANMYLLVVQQSPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPHNIL 775
++ P RVHTV++S QH E +T + + +++E VIK VIP L
Sbjct: 170 TQVTIEYINESGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYL 224
Score = 86.2 bits (204), Expect = 2e-17
Identities = 43/70 (61%), Positives = 51/70 (72%), Gaps = 4/70 (5%)
Frame = +3
Query: 120 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKT----VWCFCVAKSHP 287
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT V+ K++
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 288 KLTWIIKKLC 317
I++K C
Sbjct: 64 DYEQIVRKTC 73
Score = 61.7 bits (143), Expect = 5e-10
Identities = 29/48 (60%), Positives = 33/48 (68%)
Frame = +1
Query: 514 MPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVP 657
MPLT VLA KL K+ E+R+NG W RPD KTQVT EY+ GA VP
Sbjct: 138 MPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVP 185
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 125 bits (301), Expect = 4e-29
Identities = 71/175 (40%), Positives = 99/175 (56%), Gaps = 2/175 (1%)
Frame = +2
Query: 257 MVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHE 436
MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH
Sbjct: 50 MVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHG 109
Query: 437 N--RNDEEVGAGDQGLMFGYATDETENACR*L*CLHTNSIRKLQSSGEMENFGGQDQIQK 610
+ + EE+GAGDQG MFGYATDET L T +L + K
Sbjct: 110 HFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGK 169
Query: 611 HRLLANMYLLVVQQSPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPHNIL 775
++ Y P RVHTV++S QH E +T + + +++E VIK VIP L
Sbjct: 170 TQVTVEYYNDKGAMVPIRVHTVLISTQHDETVTNDEIARDLKEHVIKPVIPEKYL 224
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +3
Query: 120 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKT 254
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 125 bits (301), Expect = 4e-29
Identities = 71/175 (40%), Positives = 99/175 (56%), Gaps = 2/175 (1%)
Frame = +2
Query: 257 MVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHE 436
MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH
Sbjct: 50 MVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHG 109
Query: 437 N--RNDEEVGAGDQGLMFGYATDETENACR*L*CLHTNSIRKLQSSGEMENFGGQDQIQK 610
+ + EE+GAGDQG MFGYATDET L T +L + K
Sbjct: 110 HFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGK 169
Query: 611 HRLLANMYLLVVQQSPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPHNIL 775
++ Y P RVHTV++S QH E +T + + +++E VIK VIP L
Sbjct: 170 TQVTVEYYNDKGAMVPIRVHTVLISTQHDETVTNDEIARDLKEHVIKPVIPEKYL 224
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +3
Query: 120 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKT 254
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 124 bits (298), Expect = 9e-29
Identities = 68/175 (38%), Positives = 99/175 (56%), Gaps = 2/175 (1%)
Frame = +2
Query: 257 MVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHE 436
MV++ GEIT+KA +DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH
Sbjct: 50 MVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHG 109
Query: 437 N--RNDEEVGAGDQGLMFGYATDETENACR*L*CLHTNSIRKLQSSGEMENFGGQDQIQK 610
+ + E++GAGDQG MFGYATDET L T +L + K
Sbjct: 110 HFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGK 169
Query: 611 HRLLANMYLLVVQQSPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPHNIL 775
++ Y P RVHTV++S QH E +T + + +++E VIK +IP L
Sbjct: 170 TQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIARDLKEHVIKPIIPEKYL 224
Score = 86.2 bits (204), Expect = 2e-17
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +3
Query: 120 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKT 254
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48
Score = 56.4 bits (130), Expect = 2e-08
Identities = 26/48 (54%), Positives = 32/48 (66%)
Frame = +1
Query: 514 MPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVP 657
MPL+ VLA K+ ++ E+R+NG W RPD KTQVT EY GA VP
Sbjct: 138 MPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVP 185
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 120 bits (290), Expect = 8e-28
Identities = 69/175 (39%), Positives = 99/175 (56%), Gaps = 2/175 (1%)
Frame = +2
Query: 257 MVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHE 436
MV++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH
Sbjct: 50 MVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHG 109
Query: 437 N--RNDEEVGAGDQGLMFGYATDETENACR*L*CLHTNSIRKLQSSGEMENFGGQDQIQK 610
+ + E++GAGDQG MFGYATDET L T KL + + K
Sbjct: 110 HLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGK 169
Query: 611 HRLLANMYLLVVQQSPQRVHTVVVSLQHSEKITLETLRDEIREKVIKEVIPHNIL 775
++ P RVHTV++S QH E +T + + +++E VIK VIP L
Sbjct: 170 TQVTVEYKNDGGAMIPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPAKYL 224
Score = 85.0 bits (201), Expect = 5e-17
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +3
Query: 120 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKT 254
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKT 48
Score = 58.0 bits (134), Expect = 7e-09
Identities = 28/48 (58%), Positives = 32/48 (66%)
Frame = +1
Query: 514 MPLTVVLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVP 657
MPLT VLA KL K+ E+R+N W RPD KTQVT EY GGA +P
Sbjct: 138 MPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIP 185
>At5g42250.1 68418.m05143 alcohol dehydrogenase, putative similar to
alcohol dehydrogenase ADH GI:7705214 from [Lycopersicon
esculentum]; contains Pfam zinc-binding dehydrogenase
domain PF00107
Length = 390
Score = 28.3 bits (60), Expect = 6.1
Identities = 26/96 (27%), Positives = 39/96 (40%), Gaps = 4/96 (4%)
Frame = -1
Query: 660 CGDC--CTTSKYIFASNLCF*IWSCPPKFSISPELCNFLIE--FVCKHYSQRHAFSVSSV 493
CGDC C + K S F + P++ S + E F + S ++V V
Sbjct: 108 CGDCVDCKSHKSNLCSKFPFKVSPWMPRYDNSSRFTDLNGETLFHFLNVSSFSEYTVLDV 167
Query: 492 A*PNIKPWSPAPTSSSFLFSCTPAAMFGDCWSSASI 385
A +K S P S + L SC + G W +A +
Sbjct: 168 A-NVVKIDSSIPPSRACLLSCGVSTGVGAAWETAKV 202
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,806,342
Number of Sequences: 28952
Number of extensions: 431732
Number of successful extensions: 1245
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1236
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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