Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20677
         (616 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces...   106   3e-24
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    31   0.13 
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces...    26   3.8  
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    25   6.6  
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr...    25   8.7  
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo...    25   8.7  
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|...    25   8.7  

>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 195

 Score =  106 bits (254), Expect = 3e-24
 Identities = 51/93 (54%), Positives = 69/93 (74%), Gaps = 1/93 (1%)
 Frame = +3

Query: 249 KEFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRI 428
           K+F+ +HV+F+  R+ILPKP  K+RV   QKRPRSRTLT+V++AILED+VFP EI+GKR 
Sbjct: 85  KKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVHNAILEDIVFPTEIIGKRT 142

Query: 429 RVKLDGSQLIKVHLDKNQQTTIEHKVEP-SSLY 524
           R   DG + IKV LD     T+++K+   SS+Y
Sbjct: 143 RQATDGRKTIKVFLDNRDANTVDYKLGSFSSVY 175



 Score = 72.9 bits (171), Expect = 3e-14
 Identities = 37/80 (46%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
 Frame = +1

Query: 19  KIIKASGAEADSFETSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVP 192
           KI+K S ++    +  ++Q L +LE++S D+  +LR L IT A+E+E+   KK+I+++VP
Sbjct: 6   KIVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVP 65

Query: 193 MPKLKAFQKIQIRLVRELEK 252
            P LKAF K Q RL RELEK
Sbjct: 66  QPLLKAFHKCQARLTRELEK 85



 Score = 37.1 bits (82), Expect = 0.002
 Identities = 15/20 (75%), Positives = 17/20 (85%)
 Frame = +2

Query: 506 GTFQSVYKKLTGREVTFEFP 565
           G+F SVY KLTG+ VTFEFP
Sbjct: 169 GSFSSVYHKLTGKNVTFEFP 188


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 31.1 bits (67), Expect = 0.13
 Identities = 25/86 (29%), Positives = 38/86 (44%), Gaps = 1/86 (1%)
 Frame = +3

Query: 252  EFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRIR 431
            E +  H V V D+ +   P       N+Q   R  +L+ + D + +  V   E V  R  
Sbjct: 1589 EENRSHTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVR-- 1646

Query: 432  VKLDGSQLIKVHLD-KNQQTTIEHKV 506
             K  GS ++   LD K+ Q   EHK+
Sbjct: 1647 -KEAGSYILNPSLDLKHTQEMFEHKL 1671


>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 404

 Score = 26.2 bits (55), Expect = 3.8
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +2

Query: 338 KEATLKDIDLCVRCYPRGLGLPC 406
           ++AT++++D C  C  RGL + C
Sbjct: 110 RKATIRNVDYCSACGGRGLFICC 132


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 18/61 (29%), Positives = 31/61 (50%), Gaps = 5/61 (8%)
 Frame = +1

Query: 118  LRELYITKAKEIELHNKKSIIIYVPMPKLK-----AFQKIQIRLVRELEKNSVVNM*SLL 282
            L +L  +  + I  HN K    + P P LK     A +++ + LV  + K+S++   SLL
Sbjct: 1292 LEKLVFSMLRYIRFHNSKQSSKHSPSPTLKSVGPWAPRRVDV-LVTSIGKDSILEKCSLL 1350

Query: 283  E 285
            +
Sbjct: 1351 Q 1351


>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 703

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/26 (42%), Positives = 19/26 (73%)
 Frame = -3

Query: 356 P*AWPLLFVSNTSFVAGLRQDLTVSN 279
           P A  +LF+S TSF++G+ Q + ++N
Sbjct: 373 PPAAMILFISCTSFISGILQLVLLNN 398


>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
           Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 254

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +3

Query: 300 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKR 425
           P  S + R  N+++  RSR   S + + LED+++    V  R
Sbjct: 49  PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90


>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 532

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = -1

Query: 487 VCWFLSKCTLMSCEPSNLTLMR 422
           +C FL K T  SC   NL L++
Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,414,453
Number of Sequences: 5004
Number of extensions: 48663
Number of successful extensions: 134
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)