Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20673
(713 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 45 4e-05
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 43 2e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 42 3e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 42 4e-04
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 41 7e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.002
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 35 0.047
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 31 0.76
At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containi... 31 0.76
At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein ... 29 3.1
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 4.0
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 7.1
At5g50630.1 68418.m06272 nodulin family protein similar to nodul... 27 9.3
At5g50520.1 68418.m06257 nodulin family protein similar to nodul... 27 9.3
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica... 27 9.3
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 9.3
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 45.2 bits (102), Expect = 4e-05
Identities = 30/88 (34%), Positives = 47/88 (53%), Gaps = 8/88 (9%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 426
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 427 TVESRLSSDGVLTVIAPRTPAATKNERV 510
V++ + +GVLTV P+ A K +V
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKKKAQV 150
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 25/80 (31%), Positives = 46/80 (57%), Gaps = 8/80 (10%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 426
D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN +
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 427 TVESRLSSDGVLTVIAPRTP 486
+++ + +GVL+V P+ P
Sbjct: 126 EIKASM-ENGVLSVTVPKVP 144
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/83 (22%), Positives = 42/83 (50%), Gaps = 3/83 (3%)
Frame = +1
Query: 259 QVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNPDT 429
++ D+ S E++ + D ++++G+ ++ + + R + R LP+NC D
Sbjct: 139 KMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDK 198
Query: 430 VESRLSSDGVLTVIAPRTPAATK 498
+++ L +GVL + P+T K
Sbjct: 199 IKAEL-KNGVLFITIPKTKVERK 220
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.3 bits (95), Expect = 3e-04
Identities = 26/80 (32%), Positives = 45/80 (56%), Gaps = 8/80 (10%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 426
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 427 TVESRLSSDGVLTVIAPRTP 486
V++ + +GVLTV+ P+ P
Sbjct: 128 EVKATM-ENGVLTVVVPKAP 146
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 41.9 bits (94), Expect = 4e-04
Identities = 31/88 (35%), Positives = 48/88 (54%), Gaps = 8/88 (9%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 426
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 427 TVESRLSSDGVLTVIAPRTPAATKNERV 510
V++ + +GVLTV P+ A TK V
Sbjct: 122 QVKAAM-ENGVLTVTVPK--AETKKADV 146
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 41.1 bits (92), Expect = 7e-04
Identities = 28/88 (31%), Positives = 45/88 (51%), Gaps = 8/88 (9%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPD 426
D+ EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMD 121
Query: 427 TVESRLSSDGVLTVIAPRTPAATKNERV 510
V++ + +GVLTV P+ K +V
Sbjct: 122 QVKASM-ENGVLTVTVPKVETNKKKAQV 148
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 39.5 bits (88), Expect = 0.002
Identities = 25/78 (32%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
Frame = +1
Query: 271 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 426
DV EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN +
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 427 TVESRLSSDGVLTVIAPR 480
V++ + +GVL+V P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 35.1 bits (77), Expect = 0.047
Identities = 20/91 (21%), Positives = 46/91 (50%), Gaps = 9/91 (9%)
Frame = +1
Query: 256 FQVNLDVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYALP 408
+++ +V + E++ + DG + ++G H+ ++ E Y S + + +LP
Sbjct: 136 YKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLP 195
Query: 409 ENCNPDTVESRLSSDGVLTVIAPRTPAATKN 501
++ + +++ L + GVL ++ PRT KN
Sbjct: 196 DDAKVEDIKAELKN-GVLNLVIPRTEKPKKN 225
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 31.1 bits (67), Expect = 0.76
Identities = 19/72 (26%), Positives = 33/72 (45%)
Frame = +1
Query: 265 NLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRL 444
++D+ EEI V+ D ++ + F R++ LPE+ + + +
Sbjct: 40 SVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIGISAGY 99
Query: 445 SSDGVLTVIAPR 480
DGVLTVI P+
Sbjct: 100 E-DGVLTVIVPK 110
>At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 471
Score = 31.1 bits (67), Expect = 0.76
Identities = 22/74 (29%), Positives = 34/74 (45%)
Frame = +1
Query: 349 ERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERVFPSLKP 528
++ +E Y+ RR C+PDT+ R+ DG+ + A +E +F KP
Sbjct: 342 KKWEEATYLFEDMPRR-----GCSPDTLSYRIVFDGLCEGLQFEEAAVILDEMLFKGYKP 396
Query: 529 ARSGRRLRSPLRKL 570
R RL L+KL
Sbjct: 397 RRD--RLEGFLQKL 408
>At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 675
Score = 29.1 bits (62), Expect = 3.1
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +1
Query: 475 PRTPAATKNERVFPSL---KPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSV 624
P+ PA++KN + PS S +L + + + NND AVN+ +V
Sbjct: 594 PQKPASSKNLKTTPSSHIQSSLLSAMKLAAEFESAKVERGNNDPTEAVNKSNV 646
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 4.0
Identities = 16/54 (29%), Positives = 22/54 (40%)
Frame = +1
Query: 292 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSD 453
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 7.1
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +1
Query: 115 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 228
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At5g50630.1 68418.m06272 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +3
Query: 525 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 695
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At5g50520.1 68418.m06257 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.5 bits (58), Expect = 9.3
Identities = 14/57 (24%), Positives = 27/57 (47%)
Frame = +3
Query: 525 TGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYLY 695
+G + I + A ++ T + S C CYSV + C++ +++LS +Y
Sbjct: 468 SGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVY 524
>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
to GF14 protein phi chain GI:1493805, SP:P46077 from
[Arabidopsis thaliana]
Length = 267
Score = 27.5 bits (58), Expect = 9.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +3
Query: 93 RTAFAIRTSDWRLLRTIS*RPSSRQRCPEITTVRGSRWRSPIETSV 230
+ R + WR++ +I + SR +TT+R +RS IE+ +
Sbjct: 59 KNVIGARRASWRIISSIEQKEESRGNDDHVTTIRD--YRSKIESEL 102
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 9.3
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +1
Query: 292 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 468
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,176,023
Number of Sequences: 28952
Number of extensions: 281101
Number of successful extensions: 858
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 857
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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