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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20667
         (726 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_29362| Best HMM Match : No HMM Matches (HMM E-Value=.)             134   9e-32
SB_31634| Best HMM Match : Peptidase_M2 (HMM E-Value=0)               125   3e-29
SB_53557| Best HMM Match : No HMM Matches (HMM E-Value=.)             117   8e-27
SB_43454| Best HMM Match : Peptidase_M2 (HMM E-Value=0.0041)          108   5e-24
SB_6324| Best HMM Match : MCM (HMM E-Value=0)                         100   1e-21
SB_17710| Best HMM Match : Peptidase_M2 (HMM E-Value=8.8e-06)          78   6e-15
SB_53646| Best HMM Match : No HMM Matches (HMM E-Value=.)              64   1e-10
SB_16138| Best HMM Match : No HMM Matches (HMM E-Value=.)              38   0.008
SB_14754| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.9  
SB_41908| Best HMM Match : Pep_M12B_propep (HMM E-Value=1.2e-07)       28   6.7  
SB_31094| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.7  
SB_47813| Best HMM Match : HEAT (HMM E-Value=5.9e-06)                  28   8.9  

>SB_29362| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 587

 Score =  134 bits (323), Expect = 9e-32
 Identities = 57/84 (67%), Positives = 65/84 (77%)
 Frame = +1

Query: 4   KPTDREIVCHASAWDFFDGEDFRIKQCTTVDYEYFQTTHHEMGHIQYYLQYRDQPVVFRD 183
           KP  RE+ CHASAWDF    D RIKQCTT+ + Y  TTHHE+GHIQYYLQY DQP  +R 
Sbjct: 323 KPEGREVQCHASAWDFKINRDVRIKQCTTITHNYLVTTHHELGHIQYYLQYWDQPFEYRT 382

Query: 184 GANQGFHEAVGDTIALSVSSPKHL 255
           GAN GFHEAVGDT++LSV +P HL
Sbjct: 383 GANPGFHEAVGDTLSLSVDTPTHL 406



 Score = 93.9 bits (223), Expect = 1e-19
 Identities = 49/110 (44%), Positives = 69/110 (62%), Gaps = 1/110 (0%)
 Frame = +3

Query: 216 GHDSSLGVVTETSRR-VGLATGDAEDEQTEINQLYKMGIDKIAFLPFAYTLDLFRYGVFR 392
           G   SL V T T  + +GL    + D +++IN L KM + K+AFLPF + +D +R+ VF 
Sbjct: 393 GDTLSLSVDTPTHLKLIGLLDAVSNDSKSDINALMKMALRKVAFLPFGFLIDQWRWKVFS 452

Query: 393 RKTLPEDYNCHYWKLREQLQGVEPPVNRTEDDFDAAAKYTCLPTSNTPDI 542
                ++YN  +WKLR + QG++PPV RTE+DFD  AKY  +P  NTP I
Sbjct: 453 GDITSDNYNREWWKLRTKYQGIKPPVCRTENDFDPGAKYH-IP-GNTPYI 500


>SB_31634| Best HMM Match : Peptidase_M2 (HMM E-Value=0)
          Length = 663

 Score =  125 bits (302), Expect = 3e-29
 Identities = 57/91 (62%), Positives = 71/91 (78%), Gaps = 5/91 (5%)
 Frame = +1

Query: 1   EKPTDREIVCHASAWDFFD----GE-DFRIKQCTTVDYEYFQTTHHEMGHIQYYLQYRDQ 165
           EKP DRE++CHA+A DFF+    GE D RIKQCT + ++    THHEMGHI Y+L+Y DQ
Sbjct: 324 EKPEDREVLCHAAALDFFEKNSKGEKDVRIKQCTEITHQDLIITHHEMGHIYYFLKYWDQ 383

Query: 166 PVVFRDGANQGFHEAVGDTIALSVSSPKHLV 258
           P ++RDGAN  FHEA+GDTIALSV +P+HLV
Sbjct: 384 PYIYRDGANPAFHEALGDTIALSVDTPRHLV 414



 Score = 78.2 bits (184), Expect = 6e-15
 Identities = 34/75 (45%), Positives = 50/75 (66%)
 Frame = +3

Query: 285 EDEQTEINQLYKMGIDKIAFLPFAYTLDLFRYGVFRRKTLPEDYNCHYWKLREQLQGVEP 464
           ED++ EIN L KM + KIAFLPF Y L+ +R+ V+  +    +YN  +W+LR + QGV P
Sbjct: 424 EDKEAEINALMKMALSKIAFLPFGYLLEKWRWQVYTGEIDSSEYNKKWWELRVKHQGVRP 483

Query: 465 PVNRTEDDFDAAAKY 509
           P++R E  FD  +K+
Sbjct: 484 PISRDETTFDPGSKF 498



 Score = 72.1 bits (169), Expect = 4e-13
 Identities = 33/75 (44%), Positives = 47/75 (62%)
 Frame = +2

Query: 500 SQVHVSANVEYARYYVSFIIQFQFHRALCQLAGEHAAGDPNKKLVDCDIYQSVAAGNALA 679
           S+ H++AN  Y +Y++S I+QFQ H+ +C +AG       N+ L  C IY S  AG  L+
Sbjct: 496 SKFHIAANARYIQYFLSRILQFQLHKTVCDIAG------INEPLHKCSIYNSTKAGQKLS 549

Query: 680 NMLKMGSSKPWPDAM 724
            ML+MG SKPW D +
Sbjct: 550 AMLEMGRSKPWRDTL 564


>SB_53557| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1049

 Score =  117 bits (282), Expect = 8e-27
 Identities = 51/84 (60%), Positives = 64/84 (76%)
 Frame = +1

Query: 4   KPTDREIVCHASAWDFFDGEDFRIKQCTTVDYEYFQTTHHEMGHIQYYLQYRDQPVVFRD 183
           KP ++ +VCHASA+DF   +D RIK CT +  E   T HHEMGH +Y+L Y+DQP  +R+
Sbjct: 462 KPGNKSVVCHASAFDFLK-DDVRIKMCTRLKQEDLITIHHEMGHCEYFLAYKDQPYAYRN 520

Query: 184 GANQGFHEAVGDTIALSVSSPKHL 255
           GAN GFHEA+GDTIALSV +PKHL
Sbjct: 521 GANPGFHEAIGDTIALSVVTPKHL 544



 Score = 68.9 bits (161), Expect = 4e-12
 Identities = 33/84 (39%), Positives = 49/84 (58%)
 Frame = +3

Query: 291 EQTEINQLYKMGIDKIAFLPFAYTLDLFRYGVFRRKTLPEDYNCHYWKLREQLQGVEPPV 470
           ++  IN L    +  +A LPF Y +D +R+ V   +  P +YN  +W++R   QGV+PPV
Sbjct: 754 DEASINFLMTQALSHVAPLPFTYLVDQWRWKVLAGEISPSNYNSAWWRMRTLYQGVKPPV 813

Query: 471 NRTEDDFDAAAKYTCLPTSNTPDI 542
            R E DFD  AK+  +  SN+P I
Sbjct: 814 ARNESDFDPGAKFHIV--SNSPYI 835



 Score = 67.7 bits (158), Expect = 9e-12
 Identities = 33/75 (44%), Positives = 43/75 (57%)
 Frame = +2

Query: 500  SQVHVSANVEYARYYVSFIIQFQFHRALCQLAGEHAAGDPNKKLVDCDIYQSVAAGNALA 679
            ++ H+ +N  Y  Y+VS I+QFQFHRALC L  +H        L +C IYQS  AG    
Sbjct: 824  AKFHIVSNSPYIGYFVSLILQFQFHRALCNLV-KHTG-----PLHECSIYQSKEAGKKFK 877

Query: 680  NMLKMGSSKPWPDAM 724
             ML  G S+PWP  +
Sbjct: 878  EMLAAGRSRPWPQTL 892


>SB_43454| Best HMM Match : Peptidase_M2 (HMM E-Value=0.0041)
          Length = 192

 Score =  108 bits (259), Expect = 5e-24
 Identities = 55/108 (50%), Positives = 65/108 (60%), Gaps = 24/108 (22%)
 Frame = +1

Query: 4   KPTDREIVCHASAWDFFDGEDFR------------------------IKQCTTVDYEYFQ 111
           KP DR +VCHASAWDF    D R                        IKQCTT+ ++   
Sbjct: 66  KPKDRGVVCHASAWDFLISRDVRYEIQGDYVVKRGRMIAVIVTVMKVIKQCTTITHDSLV 125

Query: 112 TTHHEMGHIQYYLQYRDQPVVFRDGANQGFHEAVGDTIALSVSSPKHL 255
           TTHHE+GHIQY+LQY  QP  +R GAN GFHEAVGDT++LSV +P HL
Sbjct: 126 TTHHELGHIQYFLQYAHQPYEYRTGANPGFHEAVGDTLSLSVDTPAHL 173


>SB_6324| Best HMM Match : MCM (HMM E-Value=0)
          Length = 1592

 Score =  100 bits (239), Expect = 1e-21
 Identities = 41/61 (67%), Positives = 50/61 (81%)
 Frame = +1

Query: 73  IKQCTTVDYEYFQTTHHEMGHIQYYLQYRDQPVVFRDGANQGFHEAVGDTIALSVSSPKH 252
           IKQCTT+ ++Y  TTHHE+GHIQY+LQY  QP  +R GAN GFHEAVGDT++LSV +P H
Sbjct: 2   IKQCTTITHDYLVTTHHELGHIQYFLQYAHQPYEYRTGANPGFHEAVGDTLSLSVDTPAH 61

Query: 253 L 255
           L
Sbjct: 62  L 62



 Score = 97.5 bits (232), Expect = 1e-20
 Identities = 49/110 (44%), Positives = 72/110 (65%), Gaps = 1/110 (0%)
 Frame = +3

Query: 216 GHDSSLGVVTETS-RRVGLATGDAEDEQTEINQLYKMGIDKIAFLPFAYTLDLFRYGVFR 392
           G   SL V T     R+GL    + + +++IN L KM + K+AFLPF + +D +R+ VFR
Sbjct: 49  GDTLSLSVDTPAHLHRIGLLDAVSNNTESDINALMKMALSKVAFLPFGFLIDQWRWRVFR 108

Query: 393 RKTLPEDYNCHYWKLREQLQGVEPPVNRTEDDFDAAAKYTCLPTSNTPDI 542
            +  P++YN  +W+LR + QG++PPV RTE+DFD  AKY  +P +NTP I
Sbjct: 109 GEITPQNYNRKWWELRTKYQGIKPPVCRTENDFDPGAKYH-IP-ANTPYI 156



 Score = 29.5 bits (63), Expect = 2.9
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +2

Query: 674 LANMLKMGSSKPWPDAM 724
           ++ MLK+G SKPWP A+
Sbjct: 156 ISAMLKLGKSKPWPQAL 172


>SB_17710| Best HMM Match : Peptidase_M2 (HMM E-Value=8.8e-06)
          Length = 179

 Score = 78.2 bits (184), Expect = 6e-15
 Identities = 34/75 (45%), Positives = 50/75 (66%)
 Frame = +3

Query: 285 EDEQTEINQLYKMGIDKIAFLPFAYTLDLFRYGVFRRKTLPEDYNCHYWKLREQLQGVEP 464
           ED++ EIN L KM + KIAFLPF Y L+ +R+ V+  +    +YN  +W+LR + QGV P
Sbjct: 54  EDKEAEINALMKMALSKIAFLPFGYLLEKWRWQVYTGEIDSSEYNKKWWELRVKHQGVRP 113

Query: 465 PVNRTEDDFDAAAKY 509
           P++R E  FD  +K+
Sbjct: 114 PISRDETTFDPGSKF 128



 Score = 75.4 bits (177), Expect = 4e-14
 Identities = 31/44 (70%), Positives = 38/44 (86%)
 Frame = +1

Query: 127 MGHIQYYLQYRDQPVVFRDGANQGFHEAVGDTIALSVSSPKHLV 258
           MGHI Y+L+Y DQP ++RDGAN  FHEA+GDTIALSV +P+HLV
Sbjct: 1   MGHIYYFLKYWDQPYIYRDGANPAFHEALGDTIALSVDTPRHLV 44



 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 24/59 (40%), Positives = 35/59 (59%)
 Frame = +2

Query: 500 SQVHVSANVEYARYYVSFIIQFQFHRALCQLAGEHAAGDPNKKLVDCDIYQSVAAGNAL 676
           S+ H++AN  Y +Y++S I+QFQ H+ +C +AG       N+ L  C IY S  AG  L
Sbjct: 126 SKFHIAANARYIQYFLSRILQFQLHKTVCDIAG------INEPLHKCSIYNSTKAGQKL 178


>SB_53646| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 162

 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 30/72 (41%), Positives = 44/72 (61%)
 Frame = +3

Query: 327 IDKIAFLPFAYTLDLFRYGVFRRKTLPEDYNCHYWKLREQLQGVEPPVNRTEDDFDAAAK 506
           +  +A LPF Y +D +R+ V   +  P +YN  +W++R   QGV+PPV R E DFD  AK
Sbjct: 5   LSHVAPLPFTYLVDQWRWKVLAGEISPSNYNSAWWRMRTLYQGVKPPVARNESDFDPGAK 64

Query: 507 YTCLPTSNTPDI 542
           +  +  SN+P I
Sbjct: 65  FHIV--SNSPYI 74


>SB_16138| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 84

 Score = 37.9 bits (84), Expect = 0.008
 Identities = 27/82 (32%), Positives = 38/82 (46%), Gaps = 4/82 (4%)
 Frame = +3

Query: 303 INQLYKMGIDKIAFLPFAYTLDLFRYGVFRRKTLPEDYNCHYWKLREQLQGVEPP---VN 473
           IN L +  + K+A LP A  +DL RY V    T P  Y         ++ G   P     
Sbjct: 3   INTLIRDALQKVAILPCAVIVDLLRYRVLSGYTPPRRYKYGLVAAEARIPGCRGPRGGRG 62

Query: 474 RTEDDFD-AAAKYTCLPTSNTP 536
            TED+FD ++  + C   +NTP
Sbjct: 63  LTEDNFDPSSITHVC---NNTP 81


>SB_14754| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 145

 Score = 29.5 bits (63), Expect = 2.9
 Identities = 14/41 (34%), Positives = 25/41 (60%)
 Frame = +2

Query: 134 TSSTTCSTGINPLCSGTERTKVSTKRSGTR*LSRCRHRNIS 256
           T++T+ S   NP+  G +RT+V T  + +   S CRH +++
Sbjct: 2   TNTTSTSLHPNPIAEGYQRTEVVTDHAESE--STCRHASLA 40


>SB_41908| Best HMM Match : Pep_M12B_propep (HMM E-Value=1.2e-07)
          Length = 258

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +3

Query: 390 RRKTLPEDYNCHYWKLREQLQGVEPPVNRTED 485
           RR T PE+ N HY+K+R   + +   V +++D
Sbjct: 46  RRSTGPEEPNFHYFKVRAMGKDLHLKVTKSDD 77


>SB_31094| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1183

 Score = 28.3 bits (60), Expect = 6.7
 Identities = 10/19 (52%), Positives = 13/19 (68%)
 Frame = +3

Query: 399 TLPEDYNCHYWKLREQLQG 455
           T+ +DY+C YWK R   QG
Sbjct: 114 TIEQDYSCSYWKGRVPRQG 132


>SB_47813| Best HMM Match : HEAT (HMM E-Value=5.9e-06)
          Length = 623

 Score = 27.9 bits (59), Expect = 8.9
 Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
 Frame = +2

Query: 524 VEYARYYVSFIIQFQFHRA---LCQLAGEHAAGDPNKKLVDCDIYQSVAAGNALANMLKM 694
           V+Y + YVSF  Q +  RA   +CQL G     D ++ +  C         NA+  + +M
Sbjct: 479 VQYLKDYVSF--QTEMERAVPVICQLLGSKTTTDVSEAIEFCVTAHEFGLRNAIVGVRRM 536


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,141,290
Number of Sequences: 59808
Number of extensions: 458818
Number of successful extensions: 1510
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1350
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1499
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1937927537
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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