Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20664
(409 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 108 4e-25
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 108 4e-25
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 108 4e-25
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 67 9e-13
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 67 1e-12
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 47 1e-06
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 1e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 38 5e-04
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.003
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.003
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 30 0.16
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.28
SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomy... 25 3.4
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 4.5
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 4.5
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 4.5
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 25 6.0
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 25 6.0
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 7.9
SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual 24 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 108 bits (259), Expect = 4e-25
Identities = 49/53 (92%), Positives = 50/53 (94%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
G GSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNM
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNM 102
Score = 105 bits (251), Expect = 3e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 108 bits (259), Expect = 4e-25
Identities = 49/53 (92%), Positives = 50/53 (94%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
G GSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNM
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNM 102
Score = 105 bits (251), Expect = 3e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 108 bits (259), Expect = 4e-25
Identities = 49/53 (92%), Positives = 50/53 (94%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
G GSFKYAWVLDKLKAERERGITIDIALWKFET KY VT+IDAPGHRDFIKNM
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNM 102
Score = 105 bits (251), Expect = 3e-24
Identities = 47/51 (92%), Positives = 49/51 (96%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GK
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGK 51
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 67.3 bits (157), Expect = 9e-13
Identities = 28/48 (58%), Positives = 39/48 (81%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKD 260
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK+
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKE 283
Score = 50.0 bits (114), Expect = 1e-07
Identities = 23/63 (36%), Positives = 36/63 (57%)
Frame = +2
Query: 221 REVREGGPGNG*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFI 400
RE +E G S+ +W LD ERE+G T+++ FET +++DAPGH+ ++
Sbjct: 275 REAKEAGKE----SWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYV 330
Query: 401 KNM 409
NM
Sbjct: 331 TNM 333
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 66.9 bits (156), Expect = 1e-12
Identities = 30/54 (55%), Positives = 37/54 (68%)
Frame = +2
Query: 248 NG*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
+G GSF YAW+LD + ER RG+T+D+A FE+ K I DAPGHRDFI M
Sbjct: 219 SGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGM 272
Score = 49.6 bits (113), Expect = 2e-07
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GK
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGK 221
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 46.8 bits (106), Expect = 1e-06
Identities = 23/53 (43%), Positives = 31/53 (58%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
G SF +DK E+ RGITI A ++ET+ + +D PGH D+IKNM
Sbjct: 81 GQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNM 133
Score = 35.1 bits (77), Expect = 0.004
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +3
Query: 111 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 197
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 43.6 bits (98), Expect = 1e-05
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +2
Query: 278 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
V+D L AER+RGITI+ A F + +ID PGH DF
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADF 106
Score = 28.7 bits (61), Expect = 0.37
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCG 200
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 38.3 bits (85), Expect = 5e-04
Identities = 18/43 (41%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +2
Query: 281 LDKLKAERERGITIDI----ALWKFETSKYYVTIIDAPGHRDF 397
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDF 137
Score = 24.6 bits (51), Expect = 6.0
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 212
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.003
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 200
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.003
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 200
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 29.9 bits (64), Expect = 0.16
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +2
Query: 284 DKLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFIKN 406
+K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.28
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGI 206
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
Score = 26.2 bits (55), Expect = 2.0
Identities = 22/58 (37%), Positives = 28/58 (48%), Gaps = 19/58 (32%)
Frame = +2
Query: 281 LDKLKAERERGITIDIA----LWK---------------FETSKYYVTIIDAPGHRDF 397
+D ++ ERE+GITI A W+ FE S Y + IID PGH DF
Sbjct: 102 MDFMELEREKGITIQSAATHCTWERTVDQIEANEKQKTDFEKS-YNINIIDTPGHIDF 158
>SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 271
Score = 25.4 bits (53), Expect = 3.4
Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Frame = -1
Query: 379 SINDGNIV---LASFELPESNIDCDTTLTLSL*FVQYPSIFEGSLPISWASFS 230
S++ G + L F++P S + D+TLT L + SLP+ S +
Sbjct: 99 SVSQGGVASLELRLFDIPTSLLRSDSTLTAKLLVASFGETIPFSLPLGQLSIN 151
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 4.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 99 PKMGKEKTHINIVVIGHVDSGKST 170
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 4.5
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 246 PGPPSRTSRWYVCQYHHIC 190
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 4.5
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2
Query: 356 YYVTIIDAPGHRDF 397
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1234
Score = 24.6 bits (51), Expect = 6.0
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 3/63 (4%)
Frame = -1
Query: 406 VLDEISVSRSINDGNIVLASFELPE---SNIDCDTTLTLSL*FVQYPSIFEGSLPISWAS 236
+LDE+ S S ++ + EL E S++ C + + F S+FE S+ ++W S
Sbjct: 32 LLDELKDSYS--SPSVAIQLLELNEQAFSSLGCKLDIHIVQHFSL--SLFETSVGMNWKS 87
Query: 235 FSN 227
FSN
Sbjct: 88 FSN 90
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.6 bits (51), Expect = 6.0
Identities = 17/61 (27%), Positives = 29/61 (47%), Gaps = 12/61 (19%)
Frame = +3
Query: 135 VVIGHVDSGKSTTTGHLIYKCG-----GI-----DKRTIEK--FEKEAQEMGKDPSNMLG 278
+V+GH GK+T +Y+ G GI +R + K E+ + E+ P ++ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAGWASQNGIIGCTQPRRLVAKSVSERVSLELNSPPGSLCG 104
Query: 279 Y 281
Y
Sbjct: 105 Y 105
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 7.9
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +3
Query: 132 IVVIGHVDSGKSTTTGHLIYKC 197
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPBC13E7.05 |gpi14||pig-M|Schizosaccharomyces pombe|chr 2|||Manual
Length = 815
Score = 24.2 bits (50), Expect = 7.9
Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 4/64 (6%)
Frame = +3
Query: 111 KEKTHINIVVIGHVDS----GKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKDPSNMLG 278
KE ++N+V D S++T + + K GI + +EK + +N L
Sbjct: 288 KENVNLNLVKTPKYDDLTKMNLSSSTANTLIK--GIQSYNFQNYEKAYSLLAAGVTNSLL 345
Query: 279 YWTN 290
WTN
Sbjct: 346 EWTN 349
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,639,037
Number of Sequences: 5004
Number of extensions: 29966
Number of successful extensions: 135
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 140222766
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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