Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20664
(409 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 107 3e-24
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 107 3e-24
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 107 3e-24
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 107 3e-24
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 69 2e-12
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 60 7e-10
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 48 2e-06
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 46 1e-05
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 40 8e-04
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 40 8e-04
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 38 0.003
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 37 0.005
At5g13650.2 68418.m01585 elongation factor family protein contai... 35 0.024
At5g13650.1 68418.m01584 elongation factor family protein contai... 35 0.024
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.097
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 29 1.2
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 1.2
At5g17060.1 68418.m01999 ADP-ribosylation factor, putative simil... 27 4.8
At2g03360.1 68415.m00295 hypothetical protein contains Pfam doma... 27 4.8
At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putati... 27 6.4
At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putati... 27 6.4
At5g63710.1 68418.m07997 leucine-rich repeat transmembrane prote... 26 8.5
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 107 bits (257), Expect = 3e-24
Identities = 47/50 (94%), Positives = 49/50 (98%)
Frame = +2
Query: 260 SFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
SFKYAWVLDKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNM
Sbjct: 53 SFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNM 102
Score = 97.1 bits (231), Expect = 4e-21
Identities = 45/51 (88%), Positives = 46/51 (90%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNK 51
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 107 bits (257), Expect = 3e-24
Identities = 47/50 (94%), Positives = 49/50 (98%)
Frame = +2
Query: 260 SFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
SFKYAWVLDKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNM
Sbjct: 53 SFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNM 102
Score = 97.1 bits (231), Expect = 4e-21
Identities = 45/51 (88%), Positives = 46/51 (90%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNK 51
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 107 bits (257), Expect = 3e-24
Identities = 47/50 (94%), Positives = 49/50 (98%)
Frame = +2
Query: 260 SFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
SFKYAWVLDKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNM
Sbjct: 53 SFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNM 102
Score = 97.1 bits (231), Expect = 4e-21
Identities = 45/51 (88%), Positives = 46/51 (90%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNK 51
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 107 bits (257), Expect = 3e-24
Identities = 47/50 (94%), Positives = 49/50 (98%)
Frame = +2
Query: 260 SFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
SFKYAWVLDKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNM
Sbjct: 53 SFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNM 102
Score = 97.1 bits (231), Expect = 4e-21
Identities = 45/51 (88%), Positives = 46/51 (90%)
Frame = +3
Query: 105 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNK 51
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 68.5 bits (160), Expect = 2e-12
Identities = 27/53 (50%), Positives = 40/53 (75%)
Frame = +2
Query: 251 G*GSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
G GSF YAW LD+ ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NM
Sbjct: 282 GKGSFAYAWALDESAEERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNM 334
Score = 53.6 bits (123), Expect = 5e-08
Identities = 21/46 (45%), Positives = 34/46 (73%)
Frame = +3
Query: 120 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 257
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GK
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGK 283
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 59.7 bits (138), Expect = 7e-10
Identities = 23/49 (46%), Positives = 37/49 (75%)
Frame = +3
Query: 114 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKD 260
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ ++
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRE 146
Score = 46.4 bits (105), Expect = 7e-06
Identities = 19/50 (38%), Positives = 32/50 (64%)
Frame = +2
Query: 260 SFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
S+ A+++D + ER +G T+++ FET TI+DAPGH+ ++ NM
Sbjct: 147 SWYMAYIMDTNEEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNM 196
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 48.4 bits (110), Expect = 2e-06
Identities = 20/43 (46%), Positives = 30/43 (69%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
+DK E++RGITI A ++ET+K + +D PGH D++KNM
Sbjct: 105 IDKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNM 147
Score = 32.7 bits (71), Expect = 0.097
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +3
Query: 111 KEKTHINIVVIGHVDSGKSTTT 176
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 46.0 bits (104), Expect = 1e-05
Identities = 19/43 (44%), Positives = 27/43 (62%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNM 409
+D ER RGITI+ A ++ET + +D PGH D++KNM
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNM 159
Score = 39.1 bits (87), Expect = 0.001
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +3
Query: 102 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 236
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 39.5 bits (88), Expect = 8e-04
Identities = 19/39 (48%), Positives = 22/39 (56%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
+D + ERE+GITI A Y V IID PGH DF
Sbjct: 108 MDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDF 146
Score = 27.9 bits (59), Expect = 2.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGI 206
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 39.5 bits (88), Expect = 8e-04
Identities = 19/39 (48%), Positives = 22/39 (56%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
+D + ERE+GITI A Y V IID PGH DF
Sbjct: 108 MDSMDLEREKGITIQSAATYCTWKDYKVNIIDTPGHVDF 146
Score = 27.9 bits (59), Expect = 2.8
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGI 206
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 37.9 bits (84), Expect = 0.003
Identities = 18/39 (46%), Positives = 24/39 (61%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
+D ++ E+ERGITI A K+ + IID PGH DF
Sbjct: 136 MDWMEQEQERGITITSAATTTFWDKHRINIIDTPGHVDF 174
Score = 32.7 bits (71), Expect = 0.097
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +3
Query: 117 KTHINIVVIGHVDSGKSTTTGHLIYKCG 200
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 37.1 bits (82), Expect = 0.005
Identities = 18/43 (41%), Positives = 26/43 (60%), Gaps = 4/43 (9%)
Frame = +2
Query: 281 LDKLKAERERGITIDIAL----WKFETSKYYVTIIDAPGHRDF 397
LD + ERERGITI + + +E + + + +ID PGH DF
Sbjct: 123 LDNMDLERERGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDF 165
Score = 28.3 bits (60), Expect = 2.1
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 227
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 34.7 bits (76), Expect = 0.024
Identities = 18/40 (45%), Positives = 20/40 (50%)
Frame = +2
Query: 278 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
++D ERERGITI V IID PGH DF
Sbjct: 120 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDF 159
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 34.7 bits (76), Expect = 0.024
Identities = 18/40 (45%), Positives = 20/40 (50%)
Frame = +2
Query: 278 VLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
++D ERERGITI V IID PGH DF
Sbjct: 119 IMDSNDLERERGITILSKNTSITYKNTKVNIIDTPGHSDF 158
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 32.7 bits (71), Expect = 0.097
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGG 203
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
Score = 32.7 bits (71), Expect = 0.097
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDF 397
+D L E+ R IT+ + + Y + +ID+PGH DF
Sbjct: 49 MDYLDEEQRRAITMKSSSISLKYKDYSLNLIDSPGHMDF 87
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 29.1 bits (62), Expect = 1.2
Identities = 20/48 (41%), Positives = 24/48 (50%), Gaps = 9/48 (18%)
Frame = +2
Query: 281 LDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDF 397
LDKL +RERGIT+ E S Y + +ID PGH DF
Sbjct: 103 LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDF 148
Score = 27.5 bits (58), Expect = 3.7
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +3
Query: 102 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 212
K EK N +I H+D GKST L+ G I K
Sbjct: 60 KFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.1 bits (62), Expect = 1.2
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCG 200
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At5g17060.1 68418.m01999 ADP-ribosylation factor, putative similar
to ADP-ribosylation factor 1; ARF 1 (GP:385340)
{Drosophila melanogaster)
Length = 192
Score = 27.1 bits (57), Expect = 4.8
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3
Query: 174 TGHLIYKCGGIDKRTIEKFEKEAQEMGKDP 263
T LIY +D+ I K ++E QE+ KDP
Sbjct: 85 TDGLIYVVDSLDRERIGKAKQEFQEIIKDP 114
>At2g03360.1 68415.m00295 hypothetical protein contains Pfam domain,
PF04577: Protein of unknown function (DUF563)
Length = 393
Score = 27.1 bits (57), Expect = 4.8
Identities = 14/30 (46%), Positives = 15/30 (50%), Gaps = 2/30 (6%)
Frame = -1
Query: 94 WSYGYIDF--TFSLHKTIFFDTHHGLQTAC 11
W YID TFS HKTI D + T C
Sbjct: 137 WMPKYIDILGTFSKHKTILLDKENASITHC 166
>At3g14080.2 68416.m01780 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 6.4
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 251
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
>At3g14080.1 68416.m01779 small nuclear ribonucleoprotein, putative
/ snRNP, putative / Sm protein, putative similar to U6
snRNA-associated Sm-like protein LSm1 (Small nuclear
ribonuclear CaSm, Cancer-associated Sm-like) [Homo
sapiens] SWISS-PROT:O15116; contains Pfam profile:
PF01423 Sm protein
Length = 128
Score = 26.6 bits (56), Expect = 6.4
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +3
Query: 129 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM 251
N+V+IG +D+ + H+I + + + +K E+EA E+
Sbjct: 75 NVVLIGELDTEREELPPHMI-RVSEAEIKRAQKVEREASEL 114
>At5g63710.1 68418.m07997 leucine-rich repeat transmembrane protein
kinase, putative
Length = 614
Score = 26.2 bits (55), Expect = 8.5
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = -1
Query: 268 FEGSLPISWASFSNFSMVRLS 206
F GS+P SW+ SN + LS
Sbjct: 152 FSGSIPASWSQLSNLKHLDLS 172
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,629,386
Number of Sequences: 28952
Number of extensions: 160402
Number of successful extensions: 492
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 466
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 488
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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