Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20653
         (696 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch...   129   3e-31
SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc...    78   1e-15
SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1 |Schizosacc...    76   4e-15
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc...    38   0.002
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual    29   0.64 
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    27   1.9  
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc...    26   5.9  
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p...    25   7.8  
SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom...    25   7.8  

>SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 683

 Score =  129 bits (312), Expect = 3e-31
 Identities = 60/90 (66%), Positives = 74/90 (82%)
 Frame = +3

Query: 255 TVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPL 434
           TVGKDVS+LFPDV+  + T ++ LKKLVYLYL+NYAK+ PD+ I+AVNTFVKD E+ NP 
Sbjct: 46  TVGKDVSSLFPDVLKNLATRDITLKKLVYLYLINYAKTHPDLCILAVNTFVKDSEEYNPT 105

Query: 435 IRALAVRTMGCIRVDKITEYLCEPLESA*K 524
           +RALA+RTMGCIRV+KI  YL +PL  A K
Sbjct: 106 LRALAIRTMGCIRVNKIIGYLADPLRKALK 135



 Score = 82.6 bits (195), Expect = 5e-17
 Identities = 37/63 (58%), Positives = 48/63 (76%)
 Frame = +2

Query: 506 IRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAVAALS 685
           +RK LKDE PYVRK AAVCV K+YD+        GF++QL+ L+SD NP+VVANAV +L+
Sbjct: 130 LRKALKDEHPYVRKAAAVCVVKMYDLDREYCASNGFIEQLQALVSDPNPVVVANAVRSLA 189

Query: 686 EIN 694
           EI+
Sbjct: 190 EIH 192


>SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 745

 Score = 78.2 bits (184), Expect = 1e-15
 Identities = 34/75 (45%), Positives = 53/75 (70%)
 Frame = +3

Query: 261 GKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPLIR 440
           G+++S  FPDVV  + ++N E+++LV++YL+ YA+  PD+A+++VNT  K   D NPL R
Sbjct: 64  GENMSLYFPDVVKLVASENPEIRRLVHIYLLQYAEFNPDLALLSVNTVQKTLYDKNPLTR 123

Query: 441 ALAVRTMGCIRVDKI 485
           + A+R M  IRV  I
Sbjct: 124 STAIRVMSSIRVPAI 138



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 20/61 (32%), Positives = 35/61 (57%)
 Frame = +2

Query: 491 ISMRTIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANA 670
           I +  I++C+ D    VR++AA+ + K Y +  S        + +K LLSD++P+VV  A
Sbjct: 141 IVLLAIQQCITDTADRVRQSAALAITKCYSLDPSYKSQ--LEEHIKTLLSDNSPIVVPAA 198

Query: 671 V 673
           +
Sbjct: 199 L 199


>SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 677

 Score = 76.2 bits (179), Expect = 4e-15
 Identities = 36/83 (43%), Positives = 53/83 (63%)
 Frame = +3

Query: 261 GKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPLIR 440
           G D+S+LFP V++ M+++NLELKKL YLYL  YA  +P  A  AV   + D   SNP+IR
Sbjct: 45  GYDMSSLFPSVISSMESNNLELKKLCYLYLKIYASVKPTEAKRAVKLILNDIYSSNPMIR 104

Query: 441 ALAVRTMGCIRVDKITEYLCEPL 509
           +LA+RT+  + +        +P+
Sbjct: 105 SLALRTLTSVNIKNFWVAAMDPI 127



 Score = 73.7 bits (173), Expect = 2e-14
 Identities = 35/66 (53%), Positives = 46/66 (69%)
 Frame = +2

Query: 494 SMRTIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAV 673
           +M  I + L D DPYVRKTAA+ +AKLY     MVE  G +D LK++LSD + +VVAN++
Sbjct: 123 AMDPIVRLLDDTDPYVRKTAAIGIAKLYSYDKKMVESSGLIDHLKEMLSDESSVVVANSL 182

Query: 674 AALSEI 691
           AAL  I
Sbjct: 183 AALMNI 188


>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 940

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 20/64 (31%), Positives = 34/64 (53%)
 Frame = +2

Query: 503 TIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAVAAL 682
           T+R+CL+    YVRK A + V  +Y +S+ ++ D   L +   L ++S      NA+  L
Sbjct: 133 TVRQCLEHRHAYVRKNAILAVFSIYQVSNHLIPDAASLAE-DFLAAESEGTCKRNALIVL 191

Query: 683 SEIN 694
             I+
Sbjct: 192 FTID 195



 Score = 29.1 bits (62), Expect = 0.64
 Identities = 19/56 (33%), Positives = 26/56 (46%), Gaps = 5/56 (8%)
 Frame = +3

Query: 303 MQTDNLELKKLVYLY-----LMNYAKSQPDMAIMAVNTFVKDCEDSNPLIRALAVR 455
           M + N ELKKL+Y Y       N   +     I+A N+F  D +  N  IR   +R
Sbjct: 61  MPSRNKELKKLLYYYWEVCPKYNNDGTMKQEMILACNSFRNDLQHPNEFIRGATLR 116


>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1339

 Score = 29.1 bits (62), Expect = 0.64
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +3

Query: 306 QTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCE 419
           + DN   ++LVY  +M Y  +Q  ++I+  +TF +  E
Sbjct: 68  KNDNAAFRELVYNLMMRYVSTQKHLSIIERHTFFQTIE 105


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1067

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
 Frame = +1

Query: 82  KKKDPINYRTNI*NYIKMTDSKYFTT---TKKGEIFELKSELNSD 207
           KKKD  +++ N+ +    T+ KY  T     K + FEL  E N D
Sbjct: 93  KKKDNDDFKQNLKSSSNKTEEKYSATQASKSKNDEFELGGEENED 137


>SPBC30D10.10c |tor1||phosphatidylinositol kinase
            Tor1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2335

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
 Frame = +1

Query: 358  MQSHSQTWLSWQSILLSRT-VRIPIH*FELWLYGLWDA 468
            M SHS T L  Q++L+S+  +R+ I   ELW  GL +A
Sbjct: 1797 MLSHSDT-LVRQALLVSQELIRVAILWHELWYEGLEEA 1833


>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 505

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
 Frame = -3

Query: 496 RYSVILSTR--MHPIVRTAKARINGLESSQSLTKVLTAM 386
           RY  + S+R  M+P  +T +  IN L S QS  KVL  +
Sbjct: 9   RYFNLKSSRIGMNPQTKTFEGAINRLNSLQSNAKVLEVL 47


>SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 550

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 572 LYDISSSMVEDQGFLDQLKDLLS-DSNPMVVANAVAALSEI 691
           ++ ++ S + D GFL  L DLLS   N  +  +AV+ L  +
Sbjct: 321 IHPLNESPIIDAGFLRPLVDLLSCTENEEIQCHAVSTLRNL 361


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,718,009
Number of Sequences: 5004
Number of extensions: 53012
Number of successful extensions: 174
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)