Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20652
(746 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch... 129 4e-31
SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 78 1e-15
SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1 |Schizosacc... 76 5e-15
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 38 0.002
SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom... 30 0.40
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual 29 0.70
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 27 2.1
SPBC342.03 |||1,3-beta-glucanosyltransferase |Schizosaccharomyce... 27 2.1
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 26 6.6
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 25 8.7
>SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 683
Score = 129 bits (312), Expect = 4e-31
Identities = 60/90 (66%), Positives = 74/90 (82%)
Frame = +3
Query: 255 TVGKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPL 434
TVGKDVS+LFPDV+ + T ++ LKKLVYLYL+NYAK+ PD+ I+AVNTFVKD E+ NP
Sbjct: 46 TVGKDVSSLFPDVLKNLATRDITLKKLVYLYLINYAKTHPDLCILAVNTFVKDSEEYNPT 105
Query: 435 IRALAVRTMGCIRVDKITEYLCEPLESA*K 524
+RALA+RTMGCIRV+KI YL +PL A K
Sbjct: 106 LRALAIRTMGCIRVNKIIGYLADPLRKALK 135
Score = 83.4 bits (197), Expect = 3e-17
Identities = 37/64 (57%), Positives = 49/64 (76%)
Frame = +2
Query: 506 IRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAVAALS 685
+RK LKDE PYVRK AAVCV K+YD+ GF++QL+ L+SD NP+VVANAV +L+
Sbjct: 130 LRKALKDEHPYVRKAAAVCVVKMYDLDREYCASNGFIEQLQALVSDPNPVVVANAVRSLA 189
Query: 686 EINE 697
EI++
Sbjct: 190 EIHD 193
>SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 745
Score = 78.2 bits (184), Expect = 1e-15
Identities = 34/75 (45%), Positives = 53/75 (70%)
Frame = +3
Query: 261 GKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPLIR 440
G+++S FPDVV + ++N E+++LV++YL+ YA+ PD+A+++VNT K D NPL R
Sbjct: 64 GENMSLYFPDVVKLVASENPEIRRLVHIYLLQYAEFNPDLALLSVNTVQKTLYDKNPLTR 123
Query: 441 ALAVRTMGCIRVDKI 485
+ A+R M IRV I
Sbjct: 124 STAIRVMSSIRVPAI 138
Score = 37.9 bits (84), Expect = 0.002
Identities = 20/61 (32%), Positives = 35/61 (57%)
Frame = +2
Query: 491 ISMRTIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANA 670
I + I++C+ D VR++AA+ + K Y + S + +K LLSD++P+VV A
Sbjct: 141 IVLLAIQQCITDTADRVRQSAALAITKCYSLDPSYKSQ--LEEHIKTLLSDNSPIVVPAA 198
Query: 671 V 673
+
Sbjct: 199 L 199
>SPBC2G2.06c |apl1||AP-2 adaptor complex subunit Apl1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 677
Score = 76.2 bits (179), Expect = 5e-15
Identities = 36/83 (43%), Positives = 53/83 (63%)
Frame = +3
Query: 261 GKDVSALFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCEDSNPLIR 440
G D+S+LFP V++ M+++NLELKKL YLYL YA +P A AV + D SNP+IR
Sbjct: 45 GYDMSSLFPSVISSMESNNLELKKLCYLYLKIYASVKPTEAKRAVKLILNDIYSSNPMIR 104
Query: 441 ALAVRTMGCIRVDKITEYLCEPL 509
+LA+RT+ + + +P+
Sbjct: 105 SLALRTLTSVNIKNFWVAAMDPI 127
Score = 74.5 bits (175), Expect = 1e-14
Identities = 36/70 (51%), Positives = 48/70 (68%)
Frame = +2
Query: 494 SMRTIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAV 673
+M I + L D DPYVRKTAA+ +AKLY MVE G +D LK++LSD + +VVAN++
Sbjct: 123 AMDPIVRLLDDTDPYVRKTAAIGIAKLYSYDKKMVESSGLIDHLKEMLSDESSVVVANSL 182
Query: 674 AALSEINEAS 703
AAL I +S
Sbjct: 183 AALMNIVNSS 192
>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 940
Score = 37.5 bits (83), Expect = 0.002
Identities = 20/64 (31%), Positives = 34/64 (53%)
Frame = +2
Query: 503 TIRKCLKDEDPYVRKTAAVCVAKLYDISSSMVEDQGFLDQLKDLLSDSNPMVVANAVAAL 682
T+R+CL+ YVRK A + V +Y +S+ ++ D L + L ++S NA+ L
Sbjct: 133 TVRQCLEHRHAYVRKNAILAVFSIYQVSNHLIPDAASLAE-DFLAAESEGTCKRNALIVL 191
Query: 683 SEIN 694
I+
Sbjct: 192 FTID 195
Score = 29.1 bits (62), Expect = 0.70
Identities = 19/56 (33%), Positives = 26/56 (46%), Gaps = 5/56 (8%)
Frame = +3
Query: 303 MQTDNLELKKLVYLY-----LMNYAKSQPDMAIMAVNTFVKDCEDSNPLIRALAVR 455
M + N ELKKL+Y Y N + I+A N+F D + N IR +R
Sbjct: 61 MPSRNKELKKLLYYYWEVCPKYNNDGTMKQEMILACNSFRNDLQHPNEFIRGATLR 116
>SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 550
Score = 29.9 bits (64), Expect = 0.40
Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +2
Query: 572 LYDISSSMVEDQGFLDQLKDLLS-DSNPMVVANAVAALSEINEASV-SGRPLVEMNA 736
++ ++ S + D GFL L DLLS N + +AV+ L + +S + R ++E NA
Sbjct: 321 IHPLNESPIIDAGFLRPLVDLLSCTENEEIQCHAVSTLRNLAASSERNKRAIIEANA 377
>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1339
Score = 29.1 bits (62), Expect = 0.70
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +3
Query: 306 QTDNLELKKLVYLYLMNYAKSQPDMAIMAVNTFVKDCE 419
+ DN ++LVY +M Y +Q ++I+ +TF + E
Sbjct: 68 KNDNAAFRELVYNLMMRYVSTQKHLSIIERHTFFQTIE 105
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
Frame = +1
Query: 82 KKKDPINYRTNI*NYIKMTDSKYFTT---TKKGEIFELKSELNSD 207
KKKD +++ N+ + T+ KY T K + FEL E N D
Sbjct: 93 KKKDNDDFKQNLKSSSNKTEEKYSATQASKSKNDEFELGGEENED 137
>SPBC342.03 |||1,3-beta-glucanosyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 456
Score = 27.5 bits (58), Expect = 2.1
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +2
Query: 608 GFLDQLKDLLSDSNPMVVANAVAALSEINEASVSGRPLVEMNA 736
G+ D++K+ + S P+ ++ EIN+ + RP E+ A
Sbjct: 238 GYYDRIKEFSNYSIPLFLSEFGCNTVEINDDTTPNRPFTEIEA 280
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.8 bits (54), Expect = 6.6
Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = +1
Query: 358 MQSHSQTWLSWQSILLSRT-VRIPIH*FELWLYGLWDA 468
M SHS T L Q++L+S+ +R+ I ELW GL +A
Sbjct: 1797 MLSHSDT-LVRQALLVSQELIRVAILWHELWYEGLEEA 1833
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 25.4 bits (53), Expect = 8.7
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = -2
Query: 496 RYSVILSTR--MHPIVRTAKARINGLESSQSLTKVLTAM 386
RY + S+R M+P +T + IN L S QS KVL +
Sbjct: 9 RYFNLKSSRIGMNPQTKTFEGAINRLNSLQSNAKVLEVL 47
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,937,686
Number of Sequences: 5004
Number of extensions: 56993
Number of successful extensions: 190
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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