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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20649
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||...    89   6e-19
SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual          69   9e-13
SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase comp...    28   1.1  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    27   2.0  
SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyce...    27   2.0  
SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces p...    27   2.6  
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb...    26   4.5  
SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces pombe...    25   7.9  
SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex ...    25   7.9  
SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca...    25   7.9  
SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase Ogm2|Schizo...    25   7.9  

>SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 654

 Score = 89.0 bits (211), Expect = 6e-19
 Identities = 43/68 (63%), Positives = 50/68 (73%), Gaps = 6/68 (8%)
 Frame = +2

Query: 494 IRCETHHKVLDMCAAPGSKTAQLIEFLHADEDK------MPTGFVMANDVDNSRCYMLVH 655
           +  E+HHKVLDMCAAPGSKTAQL+E LH    K      +P+G V+AND DN R +MLVH
Sbjct: 139 LNVESHHKVLDMCAAPGSKTAQLLEALHKPTKKEDITTLLPSGIVIANDSDNKRAHMLVH 198

Query: 656 QAKRLNSP 679
           Q KRLNSP
Sbjct: 199 QIKRLNSP 206



 Score = 71.7 bits (168), Expect = 9e-14
 Identities = 34/73 (46%), Positives = 49/73 (67%), Gaps = 1/73 (1%)
 Frame = +3

Query: 324 PVNLPWYPGGFAWQLRLTRMDIRRNEALYRLHNFLVAETAAGGVSRQEQVSMIPPVVLDV 503
           PV+LPWYP G A+ L +++  IR++  L  L  FLV ET AG ++RQE VSM+PP++L+V
Sbjct: 82  PVSLPWYPDGMAFMLDISKEVIRKSPHLKALQEFLVLETEAGDINRQESVSMVPPLLLNV 141

Query: 504 KPTIRCL-TCVLP 539
           +   + L  C  P
Sbjct: 142 ESHHKVLDMCAAP 154



 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 20/46 (43%), Positives = 29/46 (63%)
 Frame = +2

Query: 80  YEDIVRENAAFEEYYKMQKICPEEQWPVFMQTLKENLPTAFRITGS 217
           Y  ++  N  FE+YY++QK+  E+ + +  Q L E LPT FRIT S
Sbjct: 5   YVYLLDRNENFEKYYRLQKLVTEDDFILLKQKLTEQLPTTFRITAS 50


>SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 674

 Score = 68.5 bits (160), Expect = 9e-13
 Identities = 31/62 (50%), Positives = 42/62 (67%)
 Frame = +3

Query: 324 PVNLPWYPGGFAWQLRLTRMDIRRNEALYRLHNFLVAETAAGGVSRQEQVSMIPPVVLDV 503
           P  LPWYP G A+ +   +  IR++  L RL  FLV+E  AG ++RQE VSM+PP+ LDV
Sbjct: 98  PTVLPWYPDGLAYIVDAQKDVIRKSPPLKRLQRFLVSENEAGNINRQEAVSMLPPLFLDV 157

Query: 504 KP 509
           +P
Sbjct: 158 EP 159



 Score = 67.7 bits (158), Expect = 1e-12
 Identities = 39/70 (55%), Positives = 43/70 (61%), Gaps = 11/70 (15%)
 Frame = +2

Query: 503 ETHHKVLDMCAAPGSKTAQLIEFLH-------ADED----KMPTGFVMANDVDNSRCYML 649
           E HH +LDMCAAPGSKTAQLIE ++       A  D    K   G V+AND D  R  ML
Sbjct: 158 EPHHVILDMCAAPGSKTAQLIEAVYKKANIKDAAHDSKNLKSVEGLVIANDADPKRAQML 217

Query: 650 VHQAKRLNSP 679
           VHQ  RLNSP
Sbjct: 218 VHQINRLNSP 227



 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 23/54 (42%), Positives = 33/54 (61%), Gaps = 6/54 (11%)
 Frame = +2

Query: 71  RKAYED---IVRENAAFEEYYKMQKIC---PEEQWPVFMQTLKENLPTAFRITG 214
           ++AY D   IV EN  FE YYK Q +    P +++  FM+ +++ LPT FRI G
Sbjct: 12  KRAYNDKSEIVLENKQFEGYYKKQNLFRGKPNDEFDSFMEYMRKPLPTTFRICG 65


>SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase complex
           beta subunit Qcr1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 457

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
 Frame = +3

Query: 372 LTRMDIRRN-EALYRLHNFLVAETAAGGVSRQEQVSMIPPVVLDVKPTIRCLTCVLPRGL 548
           LTR D+ +  +  YR    +++  +AG +S +E V +       ++P+   L+   PRGL
Sbjct: 186 LTREDLLQYIKDNYRSDRMIIS--SAGSISHEELVKLAEKYFGHLEPSAEQLSLGAPRGL 243

Query: 549 RP 554
           +P
Sbjct: 244 KP 245


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 10/32 (31%), Positives = 20/32 (62%)
 Frame = +2

Query: 11  RKRDRKEQEKNPQEKPNGDNRKAYEDIVRENA 106
           +K  RK++E +P+ +    NR +Y D+ R ++
Sbjct: 672 KKYIRKDRELSPRGRERSSNRNSYSDLSRSSS 703


>SPCC1223.14 ||SPCC297.01|chorismate synthase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 395

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = +3

Query: 60  TGIIERLMKILFEKMPHSKNTIRCKKSAQKSSGRSSCKLS 179
           TG+ E     L  K+ H+  +I   KS +  SGR  CK++
Sbjct: 244 TGLGEPCFDKLEAKLAHAMMSIPATKSFEIGSGREGCKVA 283


>SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 608

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 16/56 (28%), Positives = 26/56 (46%)
 Frame = +2

Query: 503 ETHHKVLDMCAAPGSKTAQLIEFLHADEDKMPTGFVMANDVDNSRCYMLVHQAKRL 670
           + + ++LDM +APG K   +         +  TG + AND + +R   L     RL
Sbjct: 338 QPNERILDMSSAPGGKVTYVAAL------QKNTGIIFANDSNKARTKALSANIHRL 387


>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1778

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 6/56 (10%)
 Frame = +2

Query: 14  KRDRKEQEKNPQEKPNGDNRKAYEDIVREN----AAFEEY--YKMQKICPEEQWPV 163
           K D K QEK PQ + +G  +K    ++ ++     + EE   Y  +K+C   Q+ V
Sbjct: 766 KSDSKVQEKAPQNEADGSLKKDEHVVLSDDYWMKPSIEELSKYPKEKLCSVHQFSV 821


>SPAC2C4.06c |||rRNA methyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 455

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/15 (60%), Positives = 12/15 (80%)
 Frame = +2

Query: 518 VLDMCAAPGSKTAQL 562
           ++D CAAPG+KT  L
Sbjct: 214 IIDGCAAPGNKTTHL 228


>SPAC3H1.01c |orp3|orc3, SPAP14E8.06c|origin recognition complex
           subunit Orp3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/35 (25%), Positives = 19/35 (54%)
 Frame = +2

Query: 14  KRDRKEQEKNPQEKPNGDNRKAYEDIVRENAAFEE 118
           K + KE + +P+E P G+ ++   ++  +    EE
Sbjct: 619 KENLKEDDNHPRESPQGNLQRELNELDEDKRKLEE 653


>SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non
           catalytic subunit Arm1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 811

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/31 (35%), Positives = 14/31 (45%)
 Frame = +2

Query: 500 CETHHKVLDMCAAPGSKTAQLIEFLHADEDK 592
           CE  H  LD+    G K   L   +H D D+
Sbjct: 157 CEEFHLYLDVLLLVGDKDRALDALIHQDADR 187


>SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase
           Ogm2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 739

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 9/13 (69%), Positives = 10/13 (76%)
 Frame = -2

Query: 163 DRPLLFWADFLHL 125
           D P  FW+DFLHL
Sbjct: 533 DYPSSFWSDFLHL 545


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,732,717
Number of Sequences: 5004
Number of extensions: 54176
Number of successful extensions: 157
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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