BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20648 (689 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 113 3e-26 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 113 3e-26 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 111 7e-26 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 103 2e-23 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 97 2e-21 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 57 3e-09 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 52 8e-08 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 42 6e-05 SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 25 7.8 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 7.8 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 113 bits (271), Expect = 3e-26 Identities = 52/83 (62%), Positives = 65/83 (78%) Frame = +1 Query: 259 ERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQT 438 ERLIGDAAKNQ+ NP NT+FDAKRLIGR ++D VQ D+K +PFKV+EK+ KP +QV+ Sbjct: 46 ERLIGDAAKNQVAMNPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEF 105 Query: 439 SQGDKVFAPEEISAMVLTKMKET 507 K F PEEIS+MVL KM+E+ Sbjct: 106 KGETKTFTPEEISSMVLLKMRES 128 Score = 101 bits (243), Expect = 7e-23 Identities = 48/56 (85%), Positives = 53/56 (94%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDK 677 EA+LG KVT AVVTVPAYFND+QRQATKDAG I+GLNV+RIINEPTAAAIAYGLD+ Sbjct: 130 EAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDR 185 Score = 75.4 bits (177), Expect = 7e-15 Identities = 34/39 (87%), Positives = 35/39 (89%) Frame = +2 Query: 134 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFT 250 IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT 43 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 113 bits (271), Expect = 3e-26 Identities = 53/60 (88%), Positives = 57/60 (95%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDKKEGE 689 EAYLGK VTHAVVTVPAYFNDAQRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK + E Sbjct: 163 EAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDTE 222 Score = 104 bits (250), Expect = 1e-23 Identities = 46/83 (55%), Positives = 63/83 (75%) Frame = +1 Query: 259 ERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQT 438 ERL+G+AAKNQ +NPENT+FD KRLIGR++ ++T+ D+K FPF +V ++P V+V Sbjct: 79 ERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNV 138 Query: 439 SQGDKVFAPEEISAMVLTKMKET 507 K F PEEISAM+L+KMK+T Sbjct: 139 GGKKKKFTPEEISAMILSKMKQT 161 Score = 87.4 bits (207), Expect = 2e-18 Identities = 40/43 (93%), Positives = 41/43 (95%) Frame = +2 Query: 125 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQ 253 GTVIGIDLGTTYSCV V KNGRVEIIANDQGNRITPSYVAFT+ Sbjct: 35 GTVIGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTE 77 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 111 bits (268), Expect = 7e-26 Identities = 52/83 (62%), Positives = 64/83 (77%) Frame = +1 Query: 259 ERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQT 438 ERLIGDAAKNQ+ NP NT+FDAKRLIGR++ D VQ D+K +PFKV+ K+ KP +QV+ Sbjct: 46 ERLIGDAAKNQVAMNPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEY 105 Query: 439 SQGDKVFAPEEISAMVLTKMKET 507 K F PEEIS+MVL KM+ET Sbjct: 106 KGETKTFTPEEISSMVLMKMRET 128 Score = 104 bits (249), Expect = 1e-23 Identities = 51/61 (83%), Positives = 56/61 (91%), Gaps = 1/61 (1%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDK-KEG 686 EAYLG KVT AVVTVPAYFND+QRQATKDAG I+GLNV+RIINEPTAAAIAYGLD+ +G Sbjct: 130 EAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQG 189 Query: 687 E 689 E Sbjct: 190 E 190 Score = 75.4 bits (177), Expect = 7e-15 Identities = 34/39 (87%), Positives = 35/39 (89%) Frame = +2 Query: 134 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFT 250 IGIDLGTTYSCVG + N RVEIIANDQGNR TPSYVAFT Sbjct: 5 IGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFT 43 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 103 bits (248), Expect = 2e-23 Identities = 49/82 (59%), Positives = 58/82 (70%) Frame = +1 Query: 259 ERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQT 438 ERL+GDAAKNQ NP NTVFDAKRLIGR + D Q D+K +PFKV++ N P ++V Sbjct: 50 ERLVGDAAKNQAAMNPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNY 109 Query: 439 SQGDKVFAPEEISAMVLTKMKE 504 K F +EISAMVLTKMKE Sbjct: 110 LGEKKQFTAQEISAMVLTKMKE 131 Score = 93.1 bits (221), Expect = 3e-20 Identities = 44/57 (77%), Positives = 50/57 (87%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDKK 680 EA L K+V AV+TVPAYF+D+QR ATKDAG I+GLNV+RIINEPTAAAIAYGLD K Sbjct: 134 EAKLNKRVEKAVITVPAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAK 190 Score = 65.3 bits (152), Expect = 8e-12 Identities = 29/40 (72%), Positives = 33/40 (82%) Frame = +2 Query: 134 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQ 253 IGIDLGTTYSCV V++ VEII NDQG R TPS+VAFT+ Sbjct: 9 IGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTE 48 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 97.5 bits (232), Expect = 2e-21 Identities = 44/55 (80%), Positives = 51/55 (92%) Frame = +3 Query: 516 YLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDKK 680 YLGK V +AVVTVPAYFND+QRQATK AG I+GLNV+R++NEPTAAA+AYGLDKK Sbjct: 178 YLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK 232 Score = 75.4 bits (177), Expect = 7e-15 Identities = 38/84 (45%), Positives = 56/84 (66%) Frame = +1 Query: 256 GERLIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQHDVKFFPFKVVEKNSKPHVQVQ 435 GERL+G +AK Q NPENT F KRLIGR + + VQ D+K P+K+VE +S ++ Sbjct: 94 GERLVGVSAKRQAVINPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVE-HSNGDAWLE 152 Query: 436 TSQGDKVFAPEEISAMVLTKMKET 507 ++G K ++P +I +L+KM+ET Sbjct: 153 -ARG-KTYSPSQIGGFILSKMRET 174 Score = 53.2 bits (122), Expect = 3e-08 Identities = 24/43 (55%), Positives = 31/43 (72%) Frame = +2 Query: 125 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQ 253 G VIGIDLGTT SC+ + + ++IAN +G R TPS VAFT+ Sbjct: 50 GPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTK 92 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 56.8 bits (131), Expect = 3e-09 Identities = 25/58 (43%), Positives = 40/58 (68%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDKKE 683 EA L V+ V+++PA+F D QR+A +A I+GLN +R++N+ AAA+ YG+ K + Sbjct: 134 EAELIGSVSDVVISIPAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTD 191 Score = 41.9 bits (94), Expect = 8e-05 Identities = 29/89 (32%), Positives = 45/89 (50%), Gaps = 3/89 (3%) Frame = +1 Query: 244 LHSSGER--LIGDAAKNQLTTNPENTVFDAKRLIGREWSDQTVQH-DVKFFPFKVVEKNS 414 L S GER +G+AAK+ +N NTV KRL GR + D ++ + F K+ E + Sbjct: 42 LVSYGERSRFLGEAAKSAEASNFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDG 101 Query: 415 KPHVQVQTSQGDKVFAPEEISAMVLTKMK 501 +VQ + F+ ++ A TK+K Sbjct: 102 FVGAKVQYLNEETAFSNIQLIAAYFTKIK 130 Score = 39.5 bits (88), Expect = 4e-04 Identities = 15/39 (38%), Positives = 26/39 (66%) Frame = +2 Query: 131 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAF 247 V+GID G + + + V +N +++I N+ NR TPS V++ Sbjct: 7 VVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSY 45 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 52.0 bits (119), Expect = 8e-08 Identities = 23/51 (45%), Positives = 35/51 (68%) Frame = +3 Query: 510 EAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIA 662 E +LG KV V++VP YF DAQR+A + A +GL V+++I++P A +A Sbjct: 156 EDFLGTKVNGCVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILA 206 Score = 39.9 bits (89), Expect = 3e-04 Identities = 15/40 (37%), Positives = 27/40 (67%) Frame = +2 Query: 128 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAF 247 TVIGI G S + ++G+ +++AN++GNR PS +++ Sbjct: 25 TVIGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSY 64 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 42.3 bits (95), Expect = 6e-05 Identities = 19/51 (37%), Positives = 31/51 (60%) Frame = +3 Query: 525 KKVTHAVVTVPAYFNDAQRQATKDAGTISGLNVMRIINEPTAAAIAYGLDK 677 +K+T V+TVP +FN+ QR +A I +V+ +I++ A AI Y L + Sbjct: 158 EKITDLVLTVPPHFNELQRSILLEAARILNKHVLALIDDNVAVAIEYSLSR 208 >SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 25.4 bits (53), Expect = 7.8 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +1 Query: 256 GERLIGDAAKNQLTTNPENT 315 GE ++ D+ N TNP+NT Sbjct: 6 GENIVSDSIVNMELTNPDNT 25 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 25.4 bits (53), Expect = 7.8 Identities = 9/14 (64%), Positives = 11/14 (78%) Frame = -3 Query: 249 VKATYEGVMRFPWS 208 +K TYEG +R PWS Sbjct: 284 MKLTYEGELRAPWS 297 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,696,829 Number of Sequences: 5004 Number of extensions: 53525 Number of successful extensions: 173 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 319939482 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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