SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20647
         (630 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces...   106   2e-24
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    30   0.24 
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po...    27   3.0  
SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces...    26   3.9  
SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr...    25   9.0  
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo...    25   9.0  
SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|...    25   9.0  

>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 195

 Score =  106 bits (255), Expect = 2e-24
 Identities = 49/85 (57%), Positives = 64/85 (75%)
 Frame = +2

Query: 254 KKFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRI 433
           KKF+ +HV+F+  R+ILPKP  K+RV   QKRPRSRTLT+V++AILED+VFP EI+GKR 
Sbjct: 85  KKFADRHVIFIAQRRILPKPGRKSRVT--QKRPRSRTLTAVHNAILEDIVFPTEIIGKRT 142

Query: 434 RVKLDGSQLIKVHLDKNQQTTIEHK 508
           R   DG + IKV LD     T+++K
Sbjct: 143 RQATDGRKTIKVFLDNRDANTVDYK 167



 Score = 71.7 bits (168), Expect = 8e-14
 Identities = 36/80 (45%), Positives = 55/80 (68%), Gaps = 2/80 (2%)
 Frame = +3

Query: 24  KIIKASGAEADSFETSISQALVELETNS-DLKAQLRELYITKAKEIELHN-KKSIIIYVP 197
           KI+K S ++    +  ++Q L +LE++S D+  +LR L IT A+E+E+   KK+I+++VP
Sbjct: 6   KIVKRSSSQPTETDLLVAQCLYDLESSSKDMAKELRPLQITSAREVEVGGGKKAIVVFVP 65

Query: 198 MPKLKAFQKIQIRLVRELER 257
            P LKAF K Q RL RELE+
Sbjct: 66  QPLLKAFHKCQARLTRELEK 85



 Score = 35.1 bits (77), Expect = 0.008
 Identities = 14/21 (66%), Positives = 17/21 (80%)
 Frame = +1

Query: 508 MDTFQSVYKKLTGREVTFEFP 570
           + +F SVY KLTG+ VTFEFP
Sbjct: 168 LGSFSSVYHKLTGKNVTFEFP 188


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 30.3 bits (65), Expect = 0.24
 Identities = 24/80 (30%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
 Frame = +2

Query: 272  HVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRIRVKLDG 451
            H V V D+ +   P       N+Q   R  +L+ + D + +  V   E V  R   K  G
Sbjct: 1594 HTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVR---KEAG 1650

Query: 452  SQLIKVHLD-KNQQTTIEHK 508
            S ++   LD K+ Q   EHK
Sbjct: 1651 SYILNPSLDLKHTQEMFEHK 1670


>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 472

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 11/21 (52%), Positives = 14/21 (66%)
 Frame = -1

Query: 516 GVHLCSIVVCWFLSKCTLMSC 454
           GV+L SI++  FLS C L  C
Sbjct: 138 GVYLYSILILLFLSHCVLRCC 158


>SPAC16C9.05 |||PHD finger containing protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 404

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +1

Query: 343 KEATLKDIDLCVRCYPRGLGLPC 411
           ++AT++++D C  C  RGL + C
Sbjct: 110 RKATIRNVDYCSACGGRGLFICC 132


>SPAP7G5.03 |||conjugation protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 703

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 11/26 (42%), Positives = 19/26 (73%)
 Frame = -3

Query: 361 P*AWPLLFVSNTSFVAGLRQDLTVSN 284
           P A  +LF+S TSF++G+ Q + ++N
Sbjct: 373 PPAAMILFISCTSFISGILQLVLLNN 398


>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
           Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 254

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +2

Query: 305 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKR 430
           P  S + R  N+++  RSR   S + + LED+++    V  R
Sbjct: 49  PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90


>SPBC14F5.13c |||alkaline phosphatase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 532

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = -1

Query: 492 VCWFLSKCTLMSCEPSNLTLMR 427
           +C FL K T  SC   NL L++
Sbjct: 207 LCSFLPKSTYRSCRSDNLDLLK 228


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,416,235
Number of Sequences: 5004
Number of extensions: 48448
Number of successful extensions: 136
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -