BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20646 (634 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 115 5e-27 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 115 5e-27 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 88 9e-19 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 62 5e-11 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 54 2e-08 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 53 3e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 2e-05 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.020 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.020 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.020 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 31 0.10 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.42 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 1.3 SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 27 3.0 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 3.9 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 6.9 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 25 6.9 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 6.9 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 6.9 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 6.9 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.1 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 25 9.1 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 115 bits (277), Expect = 5e-27 Identities = 65/127 (51%), Positives = 80/127 (62%), Gaps = 1/127 (0%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 433 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 434 ALXXXXXXXXXXXQTETVLRQAIAEASSLFCFMNKMDRXXXXXXXXXXXXYQTFQRIVEN 613 AL QTETVLRQA+ E +NK+DR YQ F R+VE+ Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVES 184 Query: 614 VNVIIAT 634 VNV+I+T Sbjct: 185 VNVVIST 191 Score = 102 bits (245), Expect = 4e-23 Identities = 47/70 (67%), Positives = 57/70 (81%) Frame = +3 Query: 51 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 230 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 231 KDEQDRCIPL 260 DEQ+R + + Sbjct: 61 ADEQERGVTI 70 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 115 bits (277), Expect = 5e-27 Identities = 65/127 (51%), Positives = 80/127 (62%), Gaps = 1/127 (0%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 433 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 434 ALXXXXXXXXXXXQTETVLRQAIAEASSLFCFMNKMDRXXXXXXXXXXXXYQTFQRIVEN 613 AL QTETVLRQA+ E +NK+DR YQ F R+VE+ Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVES 184 Query: 614 VNVIIAT 634 VNV+I+T Sbjct: 185 VNVVIST 191 Score = 102 bits (245), Expect = 4e-23 Identities = 47/70 (67%), Positives = 57/70 (81%) Frame = +3 Query: 51 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 230 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 231 KDEQDRCIPL 260 DEQ+R + + Sbjct: 61 ADEQERGVTI 70 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 88.2 bits (209), Expect = 9e-19 Identities = 53/126 (42%), Positives = 72/126 (57%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 436 +KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA Sbjct: 70 MKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGA 121 Query: 437 LXXXXXXXXXXXQTETVLRQAIAEASSLFCFMNKMDRXXXXXXXXXXXXYQTFQRIVENV 616 QT TVLRQA + + +NKMDR + R+VE V Sbjct: 122 FVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDRLITELKLSPIEAHYHLLRLVEQV 181 Query: 617 NVIIAT 634 N +I T Sbjct: 182 NAVIGT 187 Score = 64.1 bits (149), Expect = 2e-11 Identities = 29/64 (45%), Positives = 43/64 (67%) Frame = +3 Query: 69 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 248 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 249 CIPL 260 I + Sbjct: 67 GITM 70 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 62.5 bits (145), Expect = 5e-11 Identities = 34/76 (44%), Positives = 42/76 (55%) Frame = +2 Query: 320 NPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQA 499 N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA+ QT TV RQ Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQM 193 Query: 500 IAEASSLFCFMNKMDR 547 F+NKMDR Sbjct: 194 RRYNVPRISFVNKMDR 209 Score = 35.1 bits (77), Expect = 0.008 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +3 Query: 57 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 188 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 53.6 bits (123), Expect = 2e-08 Identities = 27/63 (42%), Positives = 35/63 (55%) Frame = +2 Query: 359 INLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAEASSLFCFMNK 538 INLID+PGH DF+ EV ++ V DGA+ QT+ V +QA F+NK Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVIFVNK 154 Query: 539 MDR 547 MDR Sbjct: 155 MDR 157 Score = 35.1 bits (77), Expect = 0.008 Identities = 20/57 (35%), Positives = 31/57 (54%), Gaps = 3/57 (5%) Frame = +3 Query: 102 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCIPLN 263 +IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R I +N Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITIN 82 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 53.2 bits (122), Expect = 3e-08 Identities = 25/91 (27%), Positives = 40/91 (43%) Frame = +2 Query: 347 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAEASSLFC 526 K F ID+PGHVDF EV A + ++DG + T +++ AI + Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVL 266 Query: 527 FMNKMDRXXXXXXXXXXXXYQTFQRIVENVN 619 +NK+DR Y + +++ VN Sbjct: 267 VLNKVDRLILELRLPPNDAYHKLRHVIDEVN 297 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 44.0 bits (99), Expect = 2e-05 Identities = 24/83 (28%), Positives = 38/83 (45%) Frame = +2 Query: 347 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAIAEASSLFC 526 + +L+NLID+PGHVDF +EV +L +G + QT + A ++ + Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIP 181 Query: 527 FMNKMDRXXXXXXXXXXXXYQTF 595 +NK+D QTF Sbjct: 182 VLNKVDLPTADVDRTLIQVQQTF 204 Score = 43.6 bits (98), Expect = 2e-05 Identities = 25/70 (35%), Positives = 40/70 (57%) Frame = +3 Query: 75 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 254 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D K E +R Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLD--KLEVERRR 104 Query: 255 PLNLRPSLCS 284 + ++ CS Sbjct: 105 GITVKAQTCS 114 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.020 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 31.5 bits (68), Expect = 0.10 Identities = 18/57 (31%), Positives = 30/57 (52%) Frame = +3 Query: 93 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCIPLN 263 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R I ++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITIS 105 Score = 28.3 bits (60), Expect = 0.97 Identities = 15/60 (25%), Positives = 24/60 (40%), Gaps = 1/60 (1%) Frame = +2 Query: 368 IDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQT-ETVLRQAIAEASSLFCFMNKMD 544 +D PGH D+ + DGA+ QT E +L + ++NK+D Sbjct: 121 VDCPGHADYIKNMITGAATMDGAIIVVSATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 29.5 bits (63), Expect = 0.42 Identities = 20/87 (22%), Positives = 41/87 (47%) Frame = -2 Query: 525 QNRLDASAIA*RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPF 346 Q + +S+++ ++ S + + +TTT +PS + S++ +S S+ S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 345 SLFSRWSGFVMNTKSFSSSSKNIEMAV 265 S S S ++ S SSSS + + + Sbjct: 184 SSSSSSSSSSSSSSSSSSSSSSSSVPI 210 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +3 Query: 114 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR 215 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 >SPBC25H2.15 |||programmed cell death protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 26.6 bits (56), Expect = 3.0 Identities = 16/82 (19%), Positives = 39/82 (47%) Frame = +3 Query: 105 IRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCIPLNLRPSLCS 284 +++ I+H++ KS+ + V+ I + + G F+D + ++ PS S Sbjct: 112 VKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDATSANNPFSLSTDVNPSKPS 171 Query: 285 SSLKRKI*YSSQTLTSVKRVRK 350 S++ K ++++ S+ +K Sbjct: 172 SNVFSKPSFAAKAQQSITDQQK 193 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 3.9 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +3 Query: 111 NMSVIAHVDHGKSTLTDSLVSKAGII 188 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.4 bits (53), Expect = 6.9 Identities = 19/70 (27%), Positives = 33/70 (47%) Frame = -2 Query: 489 STVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMN 310 S+ S T TP + STT+ + S + S ++S S+ + + S S S + Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSS-SASSSGSISSAD 198 Query: 309 TKSFSSSSKN 280 K+ S+SS + Sbjct: 199 AKTVSASSNS 208 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -2 Query: 351 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLM 256 P S +S W+ V+NTK SFS ++M +L+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELI 199 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 6.9 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -3 Query: 107 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 12 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 6.9 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 102 SASCPSSHGSRLP*NSPF*MVYDFCSIK 19 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.4 bits (53), Expect = 6.9 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +1 Query: 136 ITASQPSRTRWFPR-PVSLLVREPERPVSLTRVRTNK 243 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 9.1 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +3 Query: 111 NMSVIAHVDHGKSTLTDSL 167 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.0 bits (52), Expect = 9.1 Identities = 17/53 (32%), Positives = 28/53 (52%) Frame = +3 Query: 30 KNHKPSKMVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII 188 K P+ +V+ V EI + K + ++ V HVD GKST+ ++ + G I Sbjct: 155 KKQNPTDLVS--VPEIFEQSNPKPVV-HLVVTGHVDSGKSTMLGRIMFELGEI 204 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,570,375 Number of Sequences: 5004 Number of extensions: 50959 Number of successful extensions: 173 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 170 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 281707720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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