Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20644
(689 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 156 3e-39
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 155 4e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 99 3e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 4e-14
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 5.9
SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch... 26 5.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.9
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 7.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 156 bits (378), Expect = 3e-39
Identities = 63/85 (74%), Positives = 80/85 (94%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR+
Sbjct: 69 SIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRR 128
Query: 435 LADQCTGLQGFLIFHSFGGGTGSGL 509
+AD C+GLQGFL+FHSFGGGTGSGL
Sbjct: 129 MADNCSGLQGFLVFHSFGGGTGSGL 153
Score = 102 bits (245), Expect = 4e-23
Identities = 43/58 (74%), Positives = 55/58 (94%)
Frame = +2
Query: 512 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEA 685
+LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA
Sbjct: 155 ALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEA 212
Score = 97.1 bits (231), Expect = 2e-21
Identities = 49/69 (71%), Positives = 51/69 (73%), Gaps = 5/69 (7%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 228
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 229 TGAGKHVPR 255
TG GK VPR
Sbjct: 60 TGQGKFVPR 68
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 155 bits (377), Expect = 4e-39
Identities = 65/84 (77%), Positives = 78/84 (92%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR+
Sbjct: 65 SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124
Query: 435 LADQCTGLQGFLIFHSFGGGTGSG 506
+AD C+GLQGFL+FHSFGGGTGSG
Sbjct: 125 IADNCSGLQGFLVFHSFGGGTGSG 148
Score = 104 bits (249), Expect = 1e-23
Identities = 45/64 (70%), Positives = 50/64 (78%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 244 HVPR 255
+VPR
Sbjct: 61 YVPR 64
Score = 97.5 bits (232), Expect = 2e-21
Identities = 41/58 (70%), Positives = 54/58 (93%)
Frame = +2
Query: 512 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEA 685
+LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+
Sbjct: 151 ALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 99 bits (238), Expect = 3e-22
Identities = 44/85 (51%), Positives = 59/85 (69%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
AV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122
Query: 435 LADQCTGLQGFLIFHSFGGGTGSGL 509
A+ C LQGF + HS GGGTGSG+
Sbjct: 123 EAEACDALQGFQLTHSLGGGTGSGM 147
Score = 56.8 bits (131), Expect = 3e-09
Identities = 24/59 (40%), Positives = 39/59 (66%)
Frame = +2
Query: 512 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAI 688
+LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+
Sbjct: 149 TLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207
Score = 46.4 bits (105), Expect = 4e-06
Identities = 23/64 (35%), Positives = 31/64 (48%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 244 HVPR 255
+VPR
Sbjct: 59 YVPR 62
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.9 bits (171), Expect = 4e-14
Identities = 36/87 (41%), Positives = 54/87 (62%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRI 428
A+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I
Sbjct: 64 AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122
Query: 429 RKLADQCTGLQGFLIFHSFGGGTGSGL 509
+ AD L+GF + HS GGTGSGL
Sbjct: 123 DREADGSDSLEGFSLLHSIAGGTGSGL 149
Score = 54.4 bits (125), Expect = 1e-08
Identities = 26/63 (41%), Positives = 38/63 (60%)
Frame = +1
Query: 67 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 246
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 247 VPR 255
+PR
Sbjct: 61 IPR 63
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/60 (38%), Positives = 36/60 (60%), Gaps = 1/60 (1%)
Frame = +2
Query: 512 SLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAI 688
S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAAL 210
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -1
Query: 299 ADLINNSRFKIDEDSRGTCLPAPVSLKKVLKESSPPPM 186
AD+IN SR+ ID S T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 5.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 143 STASSLMARCPQTRPSGVETILSTLSSARPELAST 247
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 469
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
Frame = -1
Query: 341 NKLFWMKQLSVCASADLINNSRFKI------DEDSRGTCLPAPVSLKKVLKESSPPPMVL 180
N F + + + + N RF+I D +S+ T +PA + ++ + +S P P +
Sbjct: 287 NLEFGLTPVEILGHKGNVENVRFQITDSIRTDAESKFTTIPAQLFIRSIGYKSMPLPGMK 346
Query: 179 SVGI 168
VG+
Sbjct: 347 DVGV 350
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 162 WPDAHRQDHRGWRRFFQHFLQR 227
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 53 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 175
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,953,559
Number of Sequences: 5004
Number of extensions: 63863
Number of successful extensions: 209
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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