Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20640
         (565 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe...    60   2e-10
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam...    37   0.002
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc...    34   0.013
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce...    32   0.051
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    28   0.82 
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    27   1.9  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    27   2.5  
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc...    26   3.3  
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa...    26   4.4  
SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor |Schizosacc...    26   4.4  
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||...    25   5.8  
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce...    25   5.8  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   7.7  
SPBC1A4.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    25   7.7  

>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 560

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 32/77 (41%), Positives = 45/77 (58%), Gaps = 1/77 (1%)
 Frame = +1

Query: 256 ALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGP 432
           A+S +F +P +   K LVVQ+ V  E+ ++CGG YLK+   +    +M     Y IMFGP
Sbjct: 102 AISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGEMSNSIDYRIMFGP 160

Query: 433 DICGPGTKKVHVIFSYK 483
           D CG    +VH IF +K
Sbjct: 161 DKCGV-NDRVHFIFKHK 176


>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
           Mam3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1082

 Score = 36.7 bits (81), Expect = 0.002
 Identities = 26/74 (35%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
 Frame = -2

Query: 258 SKNLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQF 82
           ++N  SSE F   S+S SL   P VN +   S    + ++ L S + S+ NF+S ++S  
Sbjct: 473 AENYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLS 529

Query: 81  IEDNASKLTTTSTT 40
           + +  S L+T STT
Sbjct: 530 LTNAKSSLSTPSTT 543


>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
           Thi1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 775

 Score = 34.3 bits (75), Expect = 0.013
 Identities = 17/43 (39%), Positives = 27/43 (62%), Gaps = 3/43 (6%)
 Frame = -2

Query: 276 FELTGESKNLASS---EVFKPLSSSGSLKNFPAVNLNFPNSFP 157
           F+LTGES N ++S   E F+P   + SL N P +N ++ ++ P
Sbjct: 674 FKLTGESSNGSNSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716


>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 32.3 bits (70), Expect = 0.051
 Identities = 21/64 (32%), Positives = 29/64 (45%)
 Frame = +1

Query: 298 PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFS 477
           P+V+ F VK  +   CG  Y+ +   +   K++  E P  I FG   CG        I S
Sbjct: 103 PIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKKCGMFDYISLSIIS 161

Query: 478 YKGK 489
           Y GK
Sbjct: 162 YDGK 165


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 28.3 bits (60), Expect = 0.82
 Identities = 15/44 (34%), Positives = 19/44 (43%)
 Frame = -2

Query: 183 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 52
           N N    F      + + S  +SGNFS   T Q   DN S  +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 36/138 (26%), Positives = 62/138 (44%), Gaps = 7/138 (5%)
 Frame = -2

Query: 426 EHNLIWSLSVHVLLLQFAVKDLEVSASTVNVLFMFDSELDY---QGFTLITERFELTGES 256
           +HNL  S +  + L+ +  +D    AS  + +F   SE        +  I+ R       
Sbjct: 28  DHNLCSSQASEMDLI-YQSQDTHSCASKQDAVFQLLSETKIPVPNRYRKISHRLSTLSNK 86

Query: 255 KNLASSEVFKPLSSSGSLKNFPAVNLN---FPNSFP-GCSLYTQLLSHESSGNFSSKNTS 88
           K L  S++ + LSSS  L N   VN     F  S P  CS  T   S++   NFS  +  
Sbjct: 87  KTL-KSQLDRFLSSSKKLHN-DDVNRGDYCFLLSTPVECSASTNSHSYDCLWNFSCNSFP 144

Query: 87  QFIEDNASKLTTTSTTAF 34
           ++   +AS+ ++ ++ ++
Sbjct: 145 EYSSYSASETSSVASYSY 162


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 19/78 (24%), Positives = 38/78 (48%)
 Frame = -2

Query: 267 TGESKNLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTS 88
           +  S+  +SS     LSS  S  +  + +    ++F   S  ++  SH SS + SS ++ 
Sbjct: 388 SSSSRPASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHNSTSSRSSSHSSSHSLSSLSSK 447

Query: 87  QFIEDNASKLTTTSTTAF 34
             +  ++S L T+S+  +
Sbjct: 448 PILTASSSSLLTSSSHTY 465


>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1190

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 12/48 (25%), Positives = 24/48 (50%)
 Frame = +2

Query: 104 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 247
           + F +D  + +W   EH  K   ++   + + FS+  DD+  + SE +
Sbjct: 49  QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95


>SPBC215.15 |sec13||COPII-coated vesicle component
           Sec13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 9/16 (56%), Positives = 11/16 (68%)
 Frame = +2

Query: 101 EEKFPDDSWESNWVYS 148
           EEKFPD +W  +W  S
Sbjct: 251 EEKFPDIAWRVSWSLS 266


>SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 539

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 15/53 (28%), Positives = 29/53 (54%)
 Frame = +2

Query: 203 SDPEDDKGLKTSEDARFLLSPVSSNRSVMRVNPW*SSSLSNMNKTLTVEADTS 361
           S+PE++K LKT E ++   SPV+  +  +  N   S++ S    ++   + T+
Sbjct: 486 SEPENEKKLKTEETSKTDDSPVTDAKPDVTSNGEPSTATSESKDSVLEPSSTT 538


>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1315

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 12/31 (38%), Positives = 14/31 (45%)
 Frame = -3

Query: 146 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 54
           C P  FP N+QE      H+    I  AS Q
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821


>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 2100

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
 Frame = -3

Query: 404  SPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERARTSHLQR-FS 228
            S C  F  +  +  LRYP   +   SC  + +  K   +    L ++E+  T  L+R   
Sbjct: 1975 SKCRYFLLTAIANQLRYPSSHTYYASCCFL-YLFKSSSNNPQELLIKEQMTTVLLERIIC 2033

Query: 227  NLCHPLGR*RTFQQ 186
            N  HP G   TF +
Sbjct: 2034 NRPHPWGLLITFTE 2047


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 11/43 (25%), Positives = 21/43 (48%), Gaps = 3/43 (6%)
 Frame = -3

Query: 128 PTNHQETS---PQRIHHNLSKIMPAS*QRPVQQLSFSIHSTTF 9
           PT H  T+   P   H N +  +P + +  +  ++   ++TTF
Sbjct: 136 PTTHVNTTTVVPPTTHANTTSFVPTTTESSIHPITTGFYNTTF 178


>SPBC1A4.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 249

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 23/79 (29%), Positives = 38/79 (48%)
 Frame = -2

Query: 258 SKNLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFI 79
           + N ASS V    + S  + NF      F N  P  SL  Q L+  S+   S ++T  ++
Sbjct: 104 TSNFASSSVTSAPTQSSHISNFTNSQKYFANDLPN-SLTDQPLAQPSA---SQRST--WL 157

Query: 78  EDNASKLTTTSTTAFIFDS 22
             +A+  T++ ++   FDS
Sbjct: 158 PCSAAVSTSSPSSDPFFDS 176


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,520,906
Number of Sequences: 5004
Number of extensions: 54313
Number of successful extensions: 191
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)