Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20640
(565 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 119 2e-27
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 118 3e-27
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 109 9e-25
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 65 4e-11
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 59 2e-09
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 54 5e-08
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 33 0.17
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 31 0.40
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 0.93
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 1.2
At5g35210.2 68418.m04175 peptidase M50 family protein / sterol-r... 29 1.6
At5g35210.1 68418.m04174 peptidase M50 family protein / sterol-r... 29 1.6
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 29 2.1
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 2.8
At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger) fa... 29 2.8
At1g11300.1 68414.m01298 S-locus lectin protein kinase family pr... 29 2.8
At4g15180.1 68417.m02328 SET domain-containing protein contains ... 28 3.7
At4g35760.1 68417.m05076 expressed protein 27 6.5
At5g41320.1 68418.m05022 expressed protein 27 8.7
At3g29796.1 68416.m03790 hypothetical protein 27 8.7
At2g42290.1 68415.m05235 leucine-rich repeat family protein cont... 27 8.7
At1g74670.1 68414.m08647 gibberellin-responsive protein, putativ... 27 8.7
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 119 bits (286), Expect = 2e-27
Identities = 55/98 (56%), Positives = 67/98 (68%), Gaps = 1/98 (1%)
Frame = +1
Query: 256 ALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPD 435
A+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGPD
Sbjct: 79 AISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGPD 138
Query: 436 ICGPGTKKVHVIFSYKGKNHLIKK-VSAAKMMSTHICT 546
ICG TKKVH I +Y G NHLIKK V TH+ T
Sbjct: 139 ICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYT 176
Score = 51.6 bits (118), Expect = 4e-07
Identities = 27/60 (45%), Positives = 39/60 (65%), Gaps = 2/60 (3%)
Frame = +2
Query: 80 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 253
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Score = 27.5 bits (58), Expect = 6.5
Identities = 8/21 (38%), Positives = 15/21 (71%)
Frame = +3
Query: 501 QKSIRCKDDVYTHLYTLIVKP 563
+K + C+ D TH+YT +++P
Sbjct: 161 KKEVPCETDQLTHVYTFVLRP 181
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 118 bits (284), Expect = 3e-27
Identities = 55/98 (56%), Positives = 67/98 (68%), Gaps = 1/98 (1%)
Frame = +1
Query: 256 ALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPD 435
A+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPD
Sbjct: 79 AISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPD 138
Query: 436 ICGPGTKKVHVIFSYKGKNHLIKK-VSAAKMMSTHICT 546
ICG TKKVH I +Y NHLIKK V TH+ T
Sbjct: 139 ICGYSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYT 176
Score = 51.2 bits (117), Expect = 5e-07
Identities = 28/56 (50%), Positives = 37/56 (66%), Gaps = 2/56 (3%)
Frame = +2
Query: 92 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 253
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RF
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Score = 27.9 bits (59), Expect = 5.0
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 501 QKSIRCKDDVYTHLYTLIVKP 563
+K + C+ D TH+YT I++P
Sbjct: 161 KKDVPCETDQLTHVYTFILRP 181
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 109 bits (263), Expect = 9e-25
Identities = 48/84 (57%), Positives = 63/84 (75%)
Frame = +1
Query: 256 ALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPD 435
A+S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPD
Sbjct: 85 AISAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPD 144
Query: 436 ICGPGTKKVHVIFSYKGKNHLIKK 507
ICG TKK+HVI SY+G+N+ IKK
Sbjct: 145 ICGTQTKKLHVIVSYQGQNYPIKK 168
Score = 46.4 bits (105), Expect = 1e-05
Identities = 25/56 (44%), Positives = 35/56 (62%), Gaps = 2/56 (3%)
Frame = +2
Query: 89 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDAR 250
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAK 82
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 64.9 bits (151), Expect = 4e-11
Identities = 27/56 (48%), Positives = 39/56 (69%)
Frame = +1
Query: 256 ALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIM 423
A+S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 85 AISAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
Score = 46.4 bits (105), Expect = 1e-05
Identities = 25/56 (44%), Positives = 35/56 (62%), Gaps = 2/56 (3%)
Frame = +2
Query: 89 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDAR 250
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAK 82
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 58.8 bits (136), Expect = 2e-09
Identities = 28/73 (38%), Positives = 43/73 (58%), Gaps = 3/73 (4%)
Frame = +1
Query: 274 KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICG 444
+P + + +V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG
Sbjct: 85 EPLNLKEGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG 144
Query: 445 PGTKKVHVIFSYK 483
GT KVH I +K
Sbjct: 145 -GTNKVHFILKHK 156
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 54.4 bits (125), Expect = 5e-08
Identities = 27/69 (39%), Positives = 40/69 (57%), Gaps = 3/69 (4%)
Frame = +1
Query: 286 NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTK 456
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 92 NEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-ATN 149
Query: 457 KVHVIFSYK 483
KVH I +K
Sbjct: 150 KVHFILKHK 158
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 32.7 bits (71), Expect = 0.17
Identities = 15/58 (25%), Positives = 25/58 (43%)
Frame = +1
Query: 247 EVLALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 420
E L + +F + ++F KH D GG +F L + ++ GE P E+
Sbjct: 810 ESLLMIDEFNMVNETNSQTKIEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 31.5 bits (68), Expect = 0.40
Identities = 17/66 (25%), Positives = 35/66 (53%), Gaps = 2/66 (3%)
Frame = -2
Query: 207 SLKNFPAVNLNFPNSFPGC-SLYTQLLSHES-SGNFSSKNTSQFIEDNASKLTTTSTTAF 34
S+K+ P +++ PNS P C + + + L + +G K + +I NA +L T + + +
Sbjct: 332 SIKDQPNISVRKPNSVPECLTFHLETLEWQGYAGRPEDKEIAVYILGNALRLNTATISRY 391
Query: 33 IFDSQY 16
S++
Sbjct: 392 FSSSRF 397
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 0.93
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 307 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 417
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 1.2
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -3
Query: 395 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 267
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At5g35210.2 68418.m04175 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1409
Score = 29.5 bits (63), Expect = 1.6
Identities = 13/45 (28%), Positives = 23/45 (51%)
Frame = -2
Query: 540 NVCRHHLCSGYFFDEMVLTLVTEDYVYLLGSRTTNVRAEHNLIWS 406
NV HH+ +++ +++L ED+V + R N + E WS
Sbjct: 980 NVLVHHVIMMFYYGNPLVSLSFEDFVTRIALRCLNTQMEMLKFWS 1024
>At5g35210.1 68418.m04174 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains PFam PF02163:
sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 1576
Score = 29.5 bits (63), Expect = 1.6
Identities = 13/45 (28%), Positives = 23/45 (51%)
Frame = -2
Query: 540 NVCRHHLCSGYFFDEMVLTLVTEDYVYLLGSRTTNVRAEHNLIWS 406
NV HH+ +++ +++L ED+V + R N + E WS
Sbjct: 980 NVLVHHVIMMFYYGNPLVSLSFEDFVTRIALRCLNTQMEMLKFWS 1024
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 29.1 bits (62), Expect = 2.1
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -2
Query: 135 LLSHESSGNFSSKNTSQFIEDNASKLTTTS 46
LLSH S+ N TS+ + + S+LTTTS
Sbjct: 218 LLSHNSTINIDDLRTSKAVSRSLSELTTTS 247
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 2.8
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 401 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 300
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At1g67180.1 68414.m07642 zinc finger (C3HC4-type RING finger)
family protein / BRCT domain-containing protein contains
Pfam domains PF00533: BRCA1 C Terminus (BRCT) domain and
PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 453
Score = 28.7 bits (61), Expect = 2.8
Identities = 21/68 (30%), Positives = 36/68 (52%)
Frame = +2
Query: 161 KEFGKFKLTAGKFFSDPEDDKGLKTSEDARFLLSPVSSNRSVMRVNPW*SSSLSNMNKTL 340
K+ K T K+FS+ +++ TSE A ++ V +NR +R+ SS+ NK
Sbjct: 108 KKVNKASETFDKYFSNGGENRSGSTSELATWMEKNVEANRHSVRLRTKRPSSILE-NKEN 166
Query: 341 TVEADTSR 364
+ A++SR
Sbjct: 167 SGVAESSR 174
>At1g11300.1 68414.m01298 S-locus lectin protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains S-locus glycoprotein family domain,
Pfam:PF00954
Length = 1635
Score = 28.7 bits (61), Expect = 2.8
Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
Frame = +2
Query: 107 KFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKG----LKTSEDARFLLSPVSS 274
K+P DSW N + + G +T+ K SDP + + F+++ ++
Sbjct: 151 KYPTDSWLPNMLVGTNARIGGGNVTITSWKSPSDPSPGSYTAALVLAAYPELFIMNNNNN 210
Query: 275 NRSVMRVNPW 304
N +V R PW
Sbjct: 211 NSTVWRSGPW 220
>At4g15180.1 68417.m02328 SET domain-containing protein contains
Pfam profile PF00856: SET domain
Length = 2326
Score = 28.3 bits (60), Expect = 3.7
Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 3/63 (4%)
Frame = +2
Query: 86 CDVFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPE---DDKGLKTSEDARFL 256
CD E DS W Y ++ G E G +L+ K + D +K S++ R+L
Sbjct: 613 CDTPPHEPMASDSSLGKWFYLDYYGTEHGPARLSDLKALMEQGILFSDHMIKHSDNNRWL 672
Query: 257 LSP 265
++P
Sbjct: 673 VNP 675
>At4g35760.1 68417.m05076 expressed protein
Length = 376
Score = 27.5 bits (58), Expect = 6.5
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +2
Query: 242 DARFLLSPVSSNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANW 382
D RF P+ + S + SLS+ + + T E TS S T NW
Sbjct: 36 DRRFPAIPIKCSSSEPENGEDSAPSLSSSSSSSTSEVSTSNSSTYNW 82
>At5g41320.1 68418.m05022 expressed protein
Length = 515
Score = 27.1 bits (57), Expect = 8.7
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -2
Query: 240 SEVFKPLSSSGSLKNFPAVNLNFPNSFPG 154
S +F LSSS L++FP + FP+S G
Sbjct: 366 SSLFPKLSSSSLLQSFPVPSSLFPSSSQG 394
>At3g29796.1 68416.m03790 hypothetical protein
Length = 463
Score = 27.1 bits (57), Expect = 8.7
Identities = 22/89 (24%), Positives = 42/89 (47%)
Frame = -2
Query: 438 NVRAEHNLIWSLSVHVLLLQFAVKDLEVSASTVNVLFMFDSELDYQGFTLITERFELTGE 259
NVR +++ +L + L+ F +DL V V +F E + L +FE+ GE
Sbjct: 13 NVRGKYSS-QNLRRNSELVMFPRRDLFVGEEEVCRRDLFVGEEEVCRRDLFVGKFEVCGE 71
Query: 258 SKNLASSEVFKPLSSSGSLKNFPAVNLNF 172
+ + ++ + +SS +LK + + F
Sbjct: 72 NNSSSNLSFIRENNSSANLKIYSSAKKRF 100
>At2g42290.1 68415.m05235 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 646
Score = 27.1 bits (57), Expect = 8.7
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 3/29 (10%)
Frame = -3
Query: 437 MSGPNIISYGVSP---CMSFCSSLQSKTL 360
M P+I+S VS CMSFCSSL S L
Sbjct: 1 MKLPSILSLVVSSIFLCMSFCSSLNSDGL 29
>At1g74670.1 68414.m08647 gibberellin-responsive protein, putative
similar to SP|P46690 Gibberellin-regulated protein 4
precursor {Arabidopsis thaliana} GASA4; contains Pfam
profile PF02704: Gibberellin regulated protein
Length = 101
Score = 27.1 bits (57), Expect = 8.7
Identities = 14/37 (37%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -3
Query: 401 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 300
PCM FC +K L PP C C NW T+
Sbjct: 59 PCMFFCQKCCAKCLCVPPGTYGNKQVCPCYN-NWKTQ 94
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,332,386
Number of Sequences: 28952
Number of extensions: 294960
Number of successful extensions: 866
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 861
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1082538160
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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