Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20638
(626 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 2e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 3e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 5e-22
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 1.9
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.5
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.3
At3g51290.1 68416.m05614 proline-rich family protein 28 4.4
At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase fa... 28 5.8
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 7.7
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 7.7
At2g28550.1 68415.m03468 AP2 domain-containing transcription fac... 27 7.7
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF... 27 7.7
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 105 bits (252), Expect = 2e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +3
Query: 60 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 239
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 240 IFNGKSMKISVP 275
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 103 bits (247), Expect = 1e-22
Identities = 46/85 (54%), Positives = 65/85 (76%), Gaps = 1/85 (1%)
Frame = +2
Query: 254 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLRT 430
K EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q+++
Sbjct: 69 KLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKS 128
Query: 431 DFDSGKELLCTVLKSCGEECVIAVK 505
FD GK+L+ +V+ + GEE + A+K
Sbjct: 129 GFDDGKDLVVSVMSAMGEEQINALK 153
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 101 bits (243), Expect = 3e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +3
Query: 60 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 239
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 240 IFNGKSMKISVP 275
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 100 bits (239), Expect = 9e-22
Identities = 45/85 (52%), Positives = 64/85 (75%), Gaps = 1/85 (1%)
Frame = +2
Query: 254 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 430
K EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKN 128
Query: 431 DFDSGKELLCTVLKSCGEECVIAVK 505
F+ GK+++ +V+ + GEE + A+K
Sbjct: 129 GFEEGKDIVVSVMSAMGEEQMCALK 153
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (241), Expect = 5e-22
Identities = 45/85 (52%), Positives = 63/85 (74%), Gaps = 1/85 (1%)
Frame = +2
Query: 254 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLRT 430
K EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRL 128
Query: 431 DFDSGKELLCTVLKSCGEECVIAVK 505
FD GK+++ +V+ S GEE + AVK
Sbjct: 129 GFDEGKDIVVSVMSSMGEEQICAVK 153
Score = 96.7 bits (230), Expect = 1e-20
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +3
Query: 60 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 239
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 240 IFNGKSMKISVP 275
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.5 bits (63), Expect = 1.9
Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = -2
Query: 469 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 293
+HGA + AA++V L +RD E + V+V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 292 VHVVCGGTD 266
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.5
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +2
Query: 389 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 490
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.3
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -2
Query: 160 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 53
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 28.3 bits (60), Expect = 4.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +1
Query: 37 KTQQWVTSKTHTLRPETPGPQPPSP 111
+T W T+ T ++ P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At2g33255.1 68415.m04075 haloacid dehalogenase-like hydrolase
family protein contains InterPro accession IPR005834:
Haloacid dehalogenase-like hydrolase
Length = 224
Score = 27.9 bits (59), Expect = 5.8
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = -1
Query: 341 HQRYQSVGSLRASRGVRPCCVWRDRYLHTFTIEDINPNQV 222
HQR++ + S R RP D LH + DI PN+V
Sbjct: 121 HQRFEVIFSPALGREFRPYKPNPDPLLHICSTWDIQPNEV 160
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 7.7
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 302 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 451
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 7.7
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +2
Query: 332 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 466
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At2g28550.1 68415.m03468 AP2 domain-containing transcription factor
RAP2.7 (RAP2.7) nearly identical to AP2 domain
transcription factor RAP2.7 (GI:2281639) [Arabidopsis
thaliana]
Length = 449
Score = 27.5 bits (58), Expect = 7.7
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +1
Query: 61 KTHTLRPETPGPQPP 105
+T +RP+ PGPQPP
Sbjct: 402 QTSAVRPQPPGPQPP 416
>At1g35830.1 68414.m04452 VQ motif-containing protein contains
PF05678: VQ motif
Length = 302
Score = 27.5 bits (58), Expect = 7.7
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +1
Query: 13 SFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSP 111
++L V + ++TH L P+ P P PP P
Sbjct: 60 TYLNFVNNLISDDILNQTHLLPPQPPPPPPPPP 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,384,704
Number of Sequences: 28952
Number of extensions: 322500
Number of successful extensions: 991
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 916
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 981
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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