Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20636
(703 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 63 3e-11
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 58 1e-09
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 5e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 33 0.040
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 30 0.37
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.37
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.49
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 4.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.0
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 7.9
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 25 7.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 63.3 bits (147), Expect = 3e-11
Identities = 35/97 (36%), Positives = 55/97 (56%), Gaps = 2/97 (2%)
Frame = +2
Query: 245 VKSLGQNPTESDVKKCTLHLKPDER--ISFEVFLPIYQAISKARSGDTANDFIEGLRHFD 418
++SLGQ+PT ++++ + D I F FL + K + D + E + FD
Sbjct: 38 MRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR--KMKDTDNEEEVREAFKVFD 95
Query: 419 KDGNGFISSAELRHLLSTLGEKLSDDEVEQSCRDKKT 529
KDGNG+I+ EL H+L++LGE+LS +EV R+ T
Sbjct: 96 KDGNGYITVEELTHVLTSLGERLSQEEVADMIREADT 132
Score = 35.9 bits (79), Expect = 0.006
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = +3
Query: 153 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRA 254
+++Q+AEF+EAF LFD DG I ++G +R+
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRS 40
Score = 26.6 bits (56), Expect = 3.4
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = +2
Query: 389 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 505
+F E FD+D +G I+S EL ++ +LG+ + E++
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQ 51
Score = 26.2 bits (55), Expect = 4.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +3
Query: 159 DQLAEFQEAFQLFDSRGDGKIHVAQIGDAL 248
D E +EAF++FD G+G I V ++ L
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHVL 111
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 58.0 bits (134), Expect = 1e-09
Identities = 30/88 (34%), Positives = 53/88 (60%)
Frame = +2
Query: 245 VKSLGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKD 424
+++ GQNPT +++ + L P E + E FL + + +F++G + FDKD
Sbjct: 32 LRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLNRPNGFDMPGDPEEFVKGFQVFDKD 89
Query: 425 GNGFISSAELRHLLSTLGEKLSDDEVEQ 508
G I ELR++L++LGEKLS++E+++
Sbjct: 90 ATGMIGVGELRYVLTSLGEKLSNEEMDE 117
Score = 33.1 bits (72), Expect = 0.040
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +3
Query: 174 FQEAFQLFDSRGDGKIHVAQIGDALRA 254
+++AF LFD G G+I IGD LRA
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRA 34
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 56.0 bits (129), Expect = 5e-09
Identities = 30/87 (34%), Positives = 53/87 (60%)
Frame = +2
Query: 245 VKSLGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKD 424
++SLG N T++++ K + L + I + F+ +K R ++ ++I+ R FDKD
Sbjct: 35 LRSLGINVTDAELAKLSNEL--GDAIDEKKFMSFVS--NKLRETESEEEYIKAFRVFDKD 90
Query: 425 GNGFISSAELRHLLSTLGEKLSDDEVE 505
+G+I +A+ + TLGEKLSD+EV+
Sbjct: 91 NSGYIETAKFADYMKTLGEKLSDNEVQ 117
Score = 31.1 bits (67), Expect = 0.16
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 153 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRA 254
S++Q E +EAF L+D DG I + +G LR+
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRS 37
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 33.1 bits (72), Expect = 0.040
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +2
Query: 386 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQSCR 517
ND +E FD +G I + +R LS++G+++ EVE R
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILR 160
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +2
Query: 398 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 502
E DKDG+G I +++ +L++L + S+D +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSI 86
Score = 27.5 bits (58), Expect = 2.0
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3
Query: 144 AGYSEDQLAEFQEAFQLFDSRGDGKI 221
A + Q+ E +EAF L D GDG I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNI 65
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 29.9 bits (64), Expect = 0.37
Identities = 36/124 (29%), Positives = 55/124 (44%)
Frame = +2
Query: 320 ISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDE 499
I FE +LP + GD AN+ E K +S+ ++ LL TL + L+
Sbjct: 1435 IYFEPYLPDLLPLLLTSFGDNANEVREATMDAVKQIMSQLSAFGVKLLLPTLLDGLN--- 1491
Query: 500 VEQSCRDKKTLREISTMRTLFTSSCRAEF*VIICCSTFICKLTKSVTIQLEP*WDTVNKN 679
E + R KK EI L S A + + T I KL++ +T +T NK+
Sbjct: 1492 -EYNWRSKKASVEI-----LGLMSYMAPKQLSVFLPTIIPKLSEVLTDSHSQVRNTANKS 1545
Query: 680 LLKY 691
LL++
Sbjct: 1546 LLRF 1549
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.9 bits (64), Expect = 0.37
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +3
Query: 153 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRA 254
+E+Q + EAF+LFDS D I ++ A+RA
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRA 65
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = +2
Query: 389 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQSCRD 520
D E + FD D + I ELR + LG EV + RD
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +3
Query: 159 DQLAEFQEAFQLFDSRGDGKIHVAQI 236
D L E + AF+LFD GKI + +
Sbjct: 107 DPLEEIKRAFELFDDDETGKISLRNL 132
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.5 bits (63), Expect = 0.49
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = -1
Query: 514 AGLLYLIITELLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 335
A L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1374 ATCLHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 4.5
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -2
Query: 252 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 79
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 78 SKSN 67
K+N
Sbjct: 276 IKAN 279
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.0
Identities = 22/63 (34%), Positives = 33/63 (52%), Gaps = 8/63 (12%)
Frame = +2
Query: 209 RWKDTCRSDRRC----VKSLGQNPTESDVKKCTLH----LKPDERISFEVFLPIYQAISK 364
RW CRSD RC + Q PT+ +++ TL LK E+ S++ F I QA+ +
Sbjct: 589 RWLAACRSDPRCRRLDFQHFLQRPTQR-LQRYTLELDTILKHTEQSSWD-FQLITQAVKE 646
Query: 365 ARS 373
R+
Sbjct: 647 LRA 649
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 25.4 bits (53), Expect = 7.9
Identities = 9/24 (37%), Positives = 18/24 (75%)
Frame = -1
Query: 532 ESLLVPAGLLYLIITELLSESREQ 461
E LL+P GLL++ +++L+ + R +
Sbjct: 281 EKLLLPVGLLWIRLSDLVEKQRRK 304
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 25.4 bits (53), Expect = 7.9
Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = +2
Query: 329 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK 484
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQK 696
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,722,347
Number of Sequences: 5004
Number of extensions: 55260
Number of successful extensions: 191
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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