Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20635
(781 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 114 7e-26
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 112 3e-25
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 112 3e-25
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 112 3e-25
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 110 9e-25
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot... 28 8.0
At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (... 28 8.0
At1g52990.1 68414.m05997 thioredoxin family protein similar to S... 28 8.0
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 114 bits (274), Expect = 7e-26
Identities = 52/92 (56%), Positives = 72/92 (78%)
Frame = +1
Query: 253 NDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSK 432
+DAILDA L QDP++KVACET TKT MV++ GEIT+KANVDY+++VR+T + IG+ +
Sbjct: 24 SDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADV 83
Query: 433 GFDYKTCSVMLALDQQSPNIAAGVHGTEMTRK 528
G D C V++ ++QQSP+IA GVHG +T+K
Sbjct: 84 GLDADNCKVLVNIEQQSPDIAQGVHG-HLTKK 114
Score = 56.8 bits (131), Expect = 2e-08
Identities = 28/40 (70%), Positives = 29/40 (72%)
Frame = +3
Query: 501 GAWNRNDEEVGAGDQGLMFGYATDETEECMPLTVVLXHKL 620
G + EEVGAGDQG MFGYATDET E MPLT VL KL
Sbjct: 109 GHLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKL 148
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +2
Query: 194 FLFTSESVGEGHPDKMCDQ 250
FLFTSESV EGHPDK+CDQ
Sbjct: 4 FLFTSESVNEGHPDKLCDQ 22
Score = 35.9 bits (79), Expect = 0.030
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +2
Query: 629 KLQSFRRNGEFWWARPDSKTQVXCEYV 709
KL R+NG W RPD KTQV EY+
Sbjct: 151 KLTEVRKNGTCPWLRPDGKTQVTIEYI 177
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 112 bits (269), Expect = 3e-25
Identities = 49/86 (56%), Positives = 66/86 (76%)
Frame = +1
Query: 253 NDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSK 432
+DA+LDA L QDPD+KVACET TKT MV++ GEIT+KA +DY+K+VR+T + IG+
Sbjct: 24 SDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDV 83
Query: 433 GFDYKTCSVMLALDQQSPNIAAGVHG 510
G D C V++ ++QQSP+IA GVHG
Sbjct: 84 GLDADKCKVLVNIEQQSPDIAQGVHG 109
Score = 54.0 bits (124), Expect = 1e-07
Identities = 24/40 (60%), Positives = 30/40 (75%)
Frame = +3
Query: 501 GAWNRNDEEVGAGDQGLMFGYATDETEECMPLTVVLXHKL 620
G + + E++GAGDQG MFGYATDET E MPL+ VL K+
Sbjct: 109 GHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKI 148
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +2
Query: 194 FLFTSESVGEGHPDKMCDQ 250
FLFTSESV EGHPDK+CDQ
Sbjct: 4 FLFTSESVNEGHPDKLCDQ 22
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2
Query: 629 KLQSFRRNGEFWWARPDSKTQVXCEY 706
+L R+NG W RPD KTQV EY
Sbjct: 151 RLTEVRKNGTCRWLRPDGKTQVTVEY 176
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 112 bits (269), Expect = 3e-25
Identities = 50/86 (58%), Positives = 66/86 (76%)
Frame = +1
Query: 253 NDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSK 432
+DA+LDA L QDPD+KVACET TKT MV++ GEIT+KA VDY+K+VR+T + IG+
Sbjct: 24 SDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDV 83
Query: 433 GFDYKTCSVMLALDQQSPNIAAGVHG 510
G D C V++ ++QQSP+IA GVHG
Sbjct: 84 GLDADKCKVLVNIEQQSPDIAQGVHG 109
Score = 54.8 bits (126), Expect = 6e-08
Identities = 26/40 (65%), Positives = 30/40 (75%)
Frame = +3
Query: 501 GAWNRNDEEVGAGDQGLMFGYATDETEECMPLTVVLXHKL 620
G + + EE+GAGDQG MFGYATDET E MPL+ VL KL
Sbjct: 109 GHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKL 148
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +2
Query: 194 FLFTSESVGEGHPDKMCDQ 250
FLFTSESV EGHPDK+CDQ
Sbjct: 4 FLFTSESVNEGHPDKLCDQ 22
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2
Query: 629 KLQSFRRNGEFWWARPDSKTQVXCEY 706
+L R+NG W RPD KTQV EY
Sbjct: 151 RLTEVRKNGTCAWLRPDGKTQVTVEY 176
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 112 bits (269), Expect = 3e-25
Identities = 50/86 (58%), Positives = 66/86 (76%)
Frame = +1
Query: 253 NDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSK 432
+DA+LDA L QDPD+KVACET TKT MV++ GEIT+KA VDY+K+VR+T + IG+
Sbjct: 24 SDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDV 83
Query: 433 GFDYKTCSVMLALDQQSPNIAAGVHG 510
G D C V++ ++QQSP+IA GVHG
Sbjct: 84 GLDADKCKVLVNIEQQSPDIAQGVHG 109
Score = 54.8 bits (126), Expect = 6e-08
Identities = 26/40 (65%), Positives = 30/40 (75%)
Frame = +3
Query: 501 GAWNRNDEEVGAGDQGLMFGYATDETEECMPLTVVLXHKL 620
G + + EE+GAGDQG MFGYATDET E MPL+ VL KL
Sbjct: 109 GHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKL 148
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +2
Query: 194 FLFTSESVGEGHPDKMCDQ 250
FLFTSESV EGHPDK+CDQ
Sbjct: 4 FLFTSESVNEGHPDKLCDQ 22
Score = 33.9 bits (74), Expect = 0.12
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2
Query: 629 KLQSFRRNGEFWWARPDSKTQVXCEY 706
+L R+NG W RPD KTQV EY
Sbjct: 151 RLTEVRKNGTCAWLRPDGKTQVTVEY 176
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 110 bits (265), Expect = 9e-25
Identities = 51/92 (55%), Positives = 70/92 (76%)
Frame = +1
Query: 253 NDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSK 432
+DAILDA L QDP++KVACET TKT MV++ GEIT+ A VDY+K+VR T + IG+ +
Sbjct: 24 SDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADV 83
Query: 433 GFDYKTCSVMLALDQQSPNIAAGVHGTEMTRK 528
G D C+V++ ++QQSP+IA GVHG +T+K
Sbjct: 84 GLDADKCNVLVNIEQQSPDIAQGVHG-HLTKK 114
Score = 55.2 bits (127), Expect = 5e-08
Identities = 26/40 (65%), Positives = 29/40 (72%)
Frame = +3
Query: 501 GAWNRNDEEVGAGDQGLMFGYATDETEECMPLTVVLXHKL 620
G + E++GAGDQG MFGYATDET E MPLT VL KL
Sbjct: 109 GHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKL 148
Score = 43.2 bits (97), Expect = 2e-04
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = +2
Query: 194 FLFTSESVGEGHPDKMCDQ 250
FLFTSESV EGHPDK+CDQ
Sbjct: 4 FLFTSESVNEGHPDKLCDQ 22
Score = 31.5 bits (68), Expect = 0.65
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = +2
Query: 629 KLQSFRRNGEFWWARPDSKTQVXCEY 706
KL R+N W RPD KTQV EY
Sbjct: 151 KLTEVRKNKTCPWLRPDGKTQVTVEY 176
>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
nucleotide-exchange protein 2 {Homo sapiens}; contains
Pfam profile PF01369: Sec7 domain
Length = 1756
Score = 27.9 bits (59), Expect = 8.0
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +1
Query: 286 DPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRE---TVKHIGYDDSSKGFDYKTCS 456
DP+ +A +T + G L C K+ VD++K+ RE + ++ D +S G +T S
Sbjct: 523 DPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKS 582
>At1g54960.1 68414.m06277 NPK1-related protein kinase, putative
(ANP2) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 2, partial cds GI:2342424
Length = 596
Score = 27.9 bits (59), Expect = 8.0
Identities = 13/49 (26%), Positives = 25/49 (51%)
Frame = +1
Query: 277 LNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDD 423
L+ + DAK +C+T+++ +L C + N + + V V H Y +
Sbjct: 431 LDNNTDAKKSCDTMSEISDILKCKFDENSGNGETETKVSMEVDHPSYSE 479
>At1g52990.1 68414.m05997 thioredoxin family protein similar to
SP|P48384 Thioredoxin M-type, chloroplast precursor
(TRX-M) {Pisum sativum}; contains Pfam profile PF00085:
Thioredoxin
Length = 313
Score = 27.9 bits (59), Expect = 8.0
Identities = 12/23 (52%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
Frame = -1
Query: 553 IKPWSPAPTSSSFLF-HAPQQQC 488
IK W P+ TS+ F+F H Q QC
Sbjct: 142 IKSWIPSFTSTEFIFTHREQNQC 164
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,104,014
Number of Sequences: 28952
Number of extensions: 426155
Number of successful extensions: 1089
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1089
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1746037600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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