Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20632
(687 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 190 1e-48
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 187 6e-48
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 168 4e-42
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 132 2e-31
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 100 1e-21
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 84 9e-17
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 68 6e-12
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 51 1e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 6e-04
SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08) 31 0.87
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.87
SB_52667| Best HMM Match : MRP-L47 (HMM E-Value=0.0013) 30 2.0
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.7
SB_56561| Best HMM Match : Hom_end_hint (HMM E-Value=4.1) 27 4.3
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 4.7
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.2
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 190 bits (462), Expect = 1e-48
Identities = 93/142 (65%), Positives = 110/142 (77%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ IFDAKRLIGR+++DATVQ+DMK WPF++ SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA*TFFRIINEPTAAAIA 603
VL KMKETAEAYLG+ V +AVITVPAYF + + K R+INEPTAAA+A
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATKDAGTIAGLNVLRVINEPTAAALA 182
Query: 604 YGLDKKGTGERNVLIFDLGGGT 669
YGLDK +GE+NVLIFDLGGGT
Sbjct: 183 YGLDKSLSGEKNVLIFDLGGGT 204
Score = 129 bits (312), Expect = 2e-30
Identities = 58/65 (89%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 239 MNPNN 253
+NP N
Sbjct: 61 LNPEN 65
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 187 bits (456), Expect = 6e-48
Identities = 92/142 (64%), Positives = 106/142 (74%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGRKF+D V DM HWPF V +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA*TFFRIINEPTAAAIA 603
VLTKMKETAEAYLG V +AV+TVPAYF + + K RIINEPTAAAIA
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATKDAGTIAGLNVLRIINEPTAAAIA 182
Query: 604 YGLDKKGTGERNVLIFDLGGGT 669
YGLDKK ERNVLI+DLGGGT
Sbjct: 183 YGLDKKVGVERNVLIYDLGGGT 204
Score = 134 bits (324), Expect = 6e-32
Identities = 62/65 (95%), Positives = 63/65 (96%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPEN 65
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 168 bits (408), Expect = 4e-42
Identities = 84/142 (59%), Positives = 104/142 (73%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEVSSM 423
P+ +FDAKRLIGR ++D +VQ D+K +PF+V KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 424 VLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA*TFFRIINEPTAAAIA 603
VLTKMKETAEAYLGK V +AV+TVPAYF + + K T RIINEPTAAAIA
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGAIAGLTVMRIINEPTAAAIA 880
Query: 604 YGLDKKGTGERNVLIFDLGGGT 669
YG+DKK GE+N+L+FDLGGGT
Sbjct: 881 YGMDKK-EGEKNILVFDLGGGT 901
Score = 102 bits (244), Expect = 3e-22
Identities = 47/60 (78%), Positives = 53/60 (88%), Gaps = 1/60 (1%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 253
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 132 bits (320), Expect = 2e-31
Identities = 60/65 (92%), Positives = 62/65 (95%)
Frame = +2
Query: 59 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 239 MNPNN 253
MNP N
Sbjct: 61 MNPTN 65
Score = 111 bits (268), Expect = 4e-25
Identities = 63/113 (55%), Positives = 72/113 (63%), Gaps = 25/113 (22%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEV--- 414
P IFDAKRLIGR+F+D V+ DMKHW FEV ++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 415 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYF 507
SSMVL KMKETAEAYLG V +AV+TVPAYF
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYF 175
Score = 106 bits (254), Expect = 2e-23
Identities = 57/87 (65%), Positives = 63/87 (72%)
Frame = +1
Query: 409 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA*TFFRIINEPT 588
++SSMVL KMKETAEAYLG V +AV+TVPAYF + + K RIINEPT
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATKDAGVISGLNVLRIINEPT 270
Query: 589 AAAIAYGLDKKGTGERNVLIFDLGGGT 669
AAAIAYGLDKK ERNVLIFDLGGGT
Sbjct: 271 AAAIAYGLDKKVGQERNVLIFDLGGGT 297
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 100 bits (240), Expect = 1e-21
Identities = 62/144 (43%), Positives = 82/144 (56%), Gaps = 2/144 (1%)
Frame = +1
Query: 244 PQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDG-GKPKIKVAYKGEDKTFFPEEVSS 420
P+ I+DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 421 MVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA-*TFFRIINEPTAAA 597
V+ K++ AE L TV AV++VPA F +K + ++ SLA T R+INEPTAAA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEF-DIKQRNATVKAASLAGITVLRVINEPTAAA 210
Query: 598 IAYGLDKKGTGERNVLIFDLGGGT 669
+AYGL KK G NV++ DLGGGT
Sbjct: 211 LAYGLHKK-EGVANVMVVDLGGGT 233
Score = 67.7 bits (158), Expect = 8e-12
Identities = 30/65 (46%), Positives = 44/65 (67%), Gaps = 2/65 (3%)
Frame = +2
Query: 65 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 238
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 239 MNPNN 253
+NP N
Sbjct: 90 INPKN 94
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 84.2 bits (199), Expect = 9e-17
Identities = 47/135 (34%), Positives = 76/135 (56%), Gaps = 4/135 (2%)
Frame = +1
Query: 268 KRLIGRKFEDATVQADMKHWPFEV-DSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 444
KR IGRKF D VQ ++ H P++V + I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 445 TAEAYLGKTVQNAVITVPAYFMTLKDKPQKMQVPSLA*TFFRIINEPTAAAIAYGLDKKG 624
TAE L V + VI+VP+Y+ + + + R++N+ TA ++AYG+ K+
Sbjct: 187 TAEIALKTKVTDCVISVPSYYTDRQRRCMLDASATAGLNCLRLMNDTTAVSLAYGIYKQD 246
Query: 625 ---TGERNVLIFDLG 660
RNV+ D+G
Sbjct: 247 LPTDKPRNVVFVDIG 261
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +2
Query: 77 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 232
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 68.1 bits (159), Expect = 6e-12
Identities = 49/118 (41%), Positives = 65/118 (55%), Gaps = 26/118 (22%)
Frame = +1
Query: 364 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYF------------ 507
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+F
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATKDAG 61
Query: 508 ---------MTLKDKPQ---KMQVPSLA*TFFRIINEPTAAAIAYGLDKK--GTGERN 639
T ++P + + P RIINEPTAAAIAYGLDK G ERN
Sbjct: 62 TIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLDKARGGKAERN 119
Score = 37.1 bits (82), Expect = 0.013
Identities = 16/20 (80%), Positives = 20/20 (100%)
Frame = +3
Query: 507 HDSQRQATKDAGTISGLNVL 566
+D+QRQATKDAGTI+GL+VL
Sbjct: 50 NDAQRQATKDAGTIAGLDVL 69
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 50.8 bits (116), Expect = 1e-06
Identities = 29/109 (26%), Positives = 55/109 (50%), Gaps = 4/109 (3%)
Frame = +1
Query: 358 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFMTLKDKPQKM 537
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +F + +
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAERRALLR 126
Query: 538 QVPSLA*TFFRIINEPTAAAIAYGLDKK----GTGERNVLIFDLGGGTS 672
+ +I+N TA A+ YGL ++ T E++ + +D+G ++
Sbjct: 127 AAELVGLNVLQIMNSNTAVALNYGLFQQKSFNDTLEKHFMFYDMGASST 175
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.5 bits (93), Expect = 6e-04
Identities = 18/28 (64%), Positives = 22/28 (78%)
Frame = +2
Query: 128 GKVEIIANDQGNRTTPSYVAFTDTERLI 211
G IIAND G+R TP+ VAF+DTE+LI
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLI 110
>SB_55989| Best HMM Match : zf-C2H2 (HMM E-Value=8e-08)
Length = 1011
Score = 31.1 bits (67), Expect = 0.87
Identities = 25/90 (27%), Positives = 40/90 (44%), Gaps = 4/90 (4%)
Frame = +1
Query: 283 RKFEDATVQADM----KHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKETA 450
+K+ ++ VQAD K +VD +PK K + E+ EE +M ++K
Sbjct: 626 KKWFNSRVQADSHYSGKAHLTQVDLYNMQPKFKKTKEEEEFDKLLEEADAMTQQEVKNVK 685
Query: 451 EAYLGKTVQNAVITVPAYFMTLKDKPQKMQ 540
LGK V+ + T +TL + Q Q
Sbjct: 686 NIKLGKNVKTQLYTCEPCNITLNSQKQMSQ 715
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.87
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +2
Query: 137 EIIANDQGNRTTPSYVAFT-DTERL 208
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_52667| Best HMM Match : MRP-L47 (HMM E-Value=0.0013)
Length = 119
Score = 29.9 bits (64), Expect = 2.0
Identities = 17/68 (25%), Positives = 32/68 (47%)
Frame = +1
Query: 268 KRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKET 447
K ++G + E A + + WPF+V + K + K P + +++ +TKM+E
Sbjct: 42 KLVLGER-ERAIQELSQQQWPFDVQDPALESTSKDLKETPIKETSPVQAATLNMTKMEEN 100
Query: 448 AEAYLGKT 471
+ G T
Sbjct: 101 QQELSGNT 108
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.7
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -2
Query: 245 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 147
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_56561| Best HMM Match : Hom_end_hint (HMM E-Value=4.1)
Length = 390
Score = 26.6 bits (56), Expect(2) = 4.3
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = +1
Query: 223 QEPGGDEPQQQIFDAKRLIGRKFEDATVQADMKHWPFEVDSDGGKPKIKVAYKGEDKTFF 402
+E G P+++ D + + ED ++ +K + D++ G P V Y GED TFF
Sbjct: 52 EEEGAKGPKRR--DRADRLSQTVEDEEIEEFLKST--QPDTNEGNPNENVPYPGED-TFF 106
Score = 20.6 bits (41), Expect(2) = 4.3
Identities = 7/15 (46%), Positives = 8/15 (53%)
Frame = +1
Query: 187 VHRHRASHRRCRQEP 231
VHR H RC+ P
Sbjct: 5 VHRTPTRHHRCKLNP 19
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 4.7
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2
Query: 5 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 91
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 28.3 bits (60), Expect = 6.2
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -2
Query: 452 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLSTSKGQCFMSACTVASSNLRP 279
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCP 1165
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,297,250
Number of Sequences: 59808
Number of extensions: 535393
Number of successful extensions: 1674
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1558
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1656
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1781448916
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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