BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20630 (689 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g36640.1 68417.m05200 SEC14 cytosolic factor family protein /... 39 0.004 At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein /... 38 0.006 At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein /... 38 0.006 At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein /... 38 0.006 At1g75170.1 68414.m08731 SEC14 cytosolic factor family protein /... 36 0.025 At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly... 34 0.10 At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein /... 33 0.24 At1g74740.1 68414.m08660 calcium-dependent protein kinase, putat... 32 0.31 At2g19630.1 68415.m02293 F-box family protein contains F-box dom... 31 0.54 At2g38910.1 68415.m04783 calcium-dependent protein kinase, putat... 30 1.3 At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosph... 30 1.3 At5g65140.1 68418.m08194 trehalose-6-phosphate phosphatase, puta... 30 1.7 At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 30 1.7 At1g22210.1 68414.m02777 trehalose-6-phosphate phosphatase, puta... 29 2.2 At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative / phos... 29 2.2 At3g01640.1 68416.m00095 GHMP kinase family protein contains GHM... 29 2.9 At4g30710.2 68417.m04353 expressed protein contains Pfam domain,... 29 3.8 At4g30710.1 68417.m04352 expressed protein contains Pfam domain,... 29 3.8 At3g51850.1 68416.m05686 calcium-dependent protein kinase, putat... 29 3.8 At1g35670.1 68414.m04435 calcium-dependent protein kinase 2 (CDP... 29 3.8 At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDP... 28 5.1 At4g35310.1 68417.m05019 calcium-dependent protein kinase, putat... 28 5.1 At4g09570.1 68417.m01575 calcium-dependent protein kinase, putat... 28 5.1 At2g17290.1 68415.m01997 calcium-dependent protein kinase isofor... 28 5.1 At1g18890.1 68414.m02351 calcium-dependent protein kinase 1 (CDP... 28 5.1 At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 28 6.7 At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 28 6.7 At5g60770.1 68418.m07625 high-affinity nitrate transporter, puta... 28 6.7 At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /... 28 6.7 At5g58410.1 68418.m07314 expressed protein contains similarity t... 27 8.9 At4g38230.1 68417.m05399 calcium-dependent protein kinase, putat... 27 8.9 At1g78090.1 68414.m09100 trehalose-6-phosphate phosphatase (TPPB... 27 8.9 >At4g36640.1 68417.m05200 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to polyphosphoinositide binding protein Ssh1p (GI:2739044) {Glycine max, SEC14 cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis] and to SEC14 cytosolic factor (SP:P53989) [Candida glabrata] Length = 294 Score = 38.7 bits (86), Expect = 0.004 Identities = 17/53 (32%), Positives = 28/53 (52%) Frame = +1 Query: 517 MELIKYLITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFV 675 M+ + +I + + YYP L +YN P + A ++ K L + EK+KFV Sbjct: 159 MKTTREIIHILQNYYPERLGIAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFV 211 >At1g22180.3 68414.m02773 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam profile: PF00650 CRAL/TRIO domain; similar to polyphosphoinositide binding protein Ssh1p (GI:|2739044) {Glycine max}; similar to Phosphatidylinositol Transfer Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae] Length = 249 Score = 37.9 bits (84), Expect = 0.006 Identities = 17/43 (39%), Positives = 24/43 (55%) Frame = +1 Query: 556 YYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFVTKD 684 +YP L I+YN P + + +K+VK L K K+KFV D Sbjct: 111 HYPERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSD 153 >At1g22180.2 68414.m02774 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam profile: PF00650 CRAL/TRIO domain; similar to polyphosphoinositide binding protein Ssh1p (GI:|2739044) {Glycine max}; similar to Phosphatidylinositol Transfer Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae] Length = 314 Score = 37.9 bits (84), Expect = 0.006 Identities = 17/43 (39%), Positives = 24/43 (55%) Frame = +1 Query: 556 YYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFVTKD 684 +YP L I+YN P + + +K+VK L K K+KFV D Sbjct: 176 HYPERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSD 218 >At1g22180.1 68414.m02772 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam profile: PF00650 CRAL/TRIO domain; similar to polyphosphoinositide binding protein Ssh1p (GI:|2739044) {Glycine max}; similar to Phosphatidylinositol Transfer Protein Sec14p (GI:2780955) [Saccharomyces cerevisiae] Length = 249 Score = 37.9 bits (84), Expect = 0.006 Identities = 17/43 (39%), Positives = 24/43 (55%) Frame = +1 Query: 556 YYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFVTKD 684 +YP L I+YN P + + +K+VK L K K+KFV D Sbjct: 111 HYPERLGLAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSD 153 >At1g75170.1 68414.m08731 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to polyphosphoinositide binding protein Ssh1p (GI:2739044) {Glycine max}; similar to SEC14 cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer protein) (PI/PCTP) (SP:P24859) [Kluyveromyces lactis] and to SEC14 cytosolic factor (SP:P53989) [Candida glabrata] Length = 296 Score = 35.9 bits (79), Expect = 0.025 Identities = 17/46 (36%), Positives = 26/46 (56%) Frame = +1 Query: 538 ITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFV 675 I + + +YP L +YN P + A +K+VK + AK K+KFV Sbjct: 169 INILQNHYPERLAVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFV 214 >At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / polyphosphoinositide-binding protein, putative similar to polyphosphoinositide binding protein SEC14 homolog Ssh1p (GI:2739044) {Glycine max} Length = 341 Score = 33.9 bits (74), Expect = 0.10 Identities = 16/50 (32%), Positives = 28/50 (56%) Frame = +1 Query: 517 MELIKYLITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKM 666 ++L+ + T+ YP N + N P++ SA +KVVK LL + +K+ Sbjct: 169 IKLVTIISTIDDLNYPEKTNTYYVVNAPYIFSACWKVVKPLLQERTRKKV 218 >At4g08690.1 68417.m01432 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein contains Pfam PF00650: CRAL/TRIO domain; similar to polyphosphoinositide binding protein Ssh2p (GI:2739046) [Glycine max]; similar to SEC14-like protein (GB:U82515) [D. discoideum] Length = 301 Score = 32.7 bits (71), Expect = 0.24 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +1 Query: 556 YYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFVTKD 684 +YP L + ++YN P +KV + L K K+KFV D Sbjct: 173 HYPERLAFAVLYNPPKFFEPFWKVARPFLEPKTRNKVKFVYSD 215 >At1g74740.1 68414.m08660 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Arabidopsis thaliana] gi|604880|dbj|BAA04829; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 541 Score = 32.3 bits (70), Expect = 0.31 Identities = 16/49 (32%), Positives = 30/49 (61%) Frame = +2 Query: 356 LLVFKSKMHIKGQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTG 502 LL+ + ++ G DY E ++I+ ++E +E+ ++ MFFD DG+G Sbjct: 403 LLMEVADVNGNGCLDYGEFVAVIIH-LQKMENDEHFRQAFMFFDKDGSG 450 >At2g19630.1 68415.m02293 F-box family protein contains F-box domain Pfam:PF00646 Length = 297 Score = 31.5 bits (68), Expect = 0.54 Identities = 21/54 (38%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = -2 Query: 262 DIFLQVRMSHSMSAACLASFSQWASTLFR-YFSSLVRSKSAGWKSTSFSLRRFS 104 +IFL R+S + A C QWASTL R YF+ L ++S F+ R+ S Sbjct: 24 EIFL--RLSVNSIARCRCVSKQWASTLSRPYFTELFLTRSLARPKLLFAYRKGS 75 >At2g38910.1 68415.m04783 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase, isoform AK1 (CDPK) [Arabidopsis thaliana] SWISS-PROT:Q06850; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 583 Score = 30.3 bits (65), Expect = 1.3 Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 2/80 (2%) Frame = +2 Query: 278 NEDNIRMDYVKEGVFFPRGRDVDGSLLLVFKSKMHI--KGQRDYDELKKIMIYWFDRVER 451 N +I ++ +K+G+ G D+ S +L I G DY E M++ +++E+ Sbjct: 451 NSGHITLEELKKGLDRV-GADLKDSEILGLMQAADIDNSGTIDYGEFIAAMVH-LNKIEK 508 Query: 452 EENGKKISMFFDMDGTGLST 511 E++ +FD DG+G T Sbjct: 509 EDHLFTAFSYFDQDGSGYIT 528 >At1g55840.1 68414.m06404 SEC14 cytosolic factor (SEC14) / phosphoglyceride transfer protein similar to polyphosphoinositide binding protein SEC14 homolog Ssh1p (GB:AAB94598) [Glycine max]; identified in Eur J Biochem 1998 Dec 1;258(2):402-10 as AtSEC14, characterized by functional complementation in S. cerevisiae. Length = 325 Score = 30.3 bits (65), Expect = 1.3 Identities = 13/51 (25%), Positives = 28/51 (54%) Frame = +1 Query: 517 MELIKYLITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMK 669 ++L+ + T+ YP + N+P++ SA +K +K LL + +K++ Sbjct: 169 IKLMTAITTIDDLNYPEKTETYYVVNVPYIFSACWKTIKPLLQERTKKKIQ 219 >At5g65140.1 68418.m08194 trehalose-6-phosphate phosphatase, putative similar to trehalose-6-phosphate phosphatase (AtTPPB) [Arabidopsis thaliana] GI:2944180; contains Pfam profile PF02358: Trehalose-phosphatase Length = 370 Score = 29.9 bits (64), Expect = 1.7 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = +2 Query: 434 FDRVEREENGKKISMFFDMDGT 499 F+R+ E GK+I MF D DGT Sbjct: 104 FERIIEEARGKQIVMFLDYDGT 125 >At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to polyphosphoinositide binding protein Ssh1p (GI:2739044) {Glycine max}; similar to polyphosphoinositide binding protein Ssh2, Glycine max, gb:T05953; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 668 Score = 29.9 bits (64), Expect = 1.7 Identities = 12/48 (25%), Positives = 25/48 (52%) Frame = +1 Query: 529 KYLITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKF 672 K + L + YP F++ I N+PW A ++++ + ++ K+ F Sbjct: 474 KQALHLLQDNYPEFVSKQIFINVPWWYLAFYRIISPFMSQRSKSKLVF 521 >At1g22210.1 68414.m02777 trehalose-6-phosphate phosphatase, putative similar to trehalose-6-phosphate phosphatase (AtTPPB) GI:2944180 from [Arabidopsis thaliana]; contains Pfam profile PF02358: Trehalose-phosphatase Length = 320 Score = 29.5 bits (63), Expect = 2.2 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +2 Query: 434 FDRVEREENGKKISMFFDMDGT 499 F+++ R+ GK+I MF D DGT Sbjct: 55 FEQIMRDAEGKQIIMFLDYDGT 76 >At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative / phosphoglyceride transfer protein, putative contains Pfam PF00650 : CRAL/TRIO domain and PF03765 : CRAL/TRIO, N-terminus; similar to polyphosphoinositide binding protein Ssh2p GB:AAB94599 GI:2739046 from [Glycine max] Length = 255 Score = 29.5 bits (63), Expect = 2.2 Identities = 16/47 (34%), Positives = 25/47 (53%) Frame = +1 Query: 535 LITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFV 675 L TL CY P L + I + P++ A+KV+ + A +K+ FV Sbjct: 176 LSTLQDCY-PERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVFV 221 >At3g01640.1 68416.m00095 GHMP kinase family protein contains GHMP kinases putative ATP-binding protein domain, Pfam:PF00288 Length = 362 Score = 29.1 bits (62), Expect = 2.9 Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 6/58 (10%) Frame = +3 Query: 12 LTQFKTISCCLQ*YNRFISNHGYCGGR------AEIFENRLKENDVDFHPADLERTRD 167 L QF ++ L NR + N GY GG ++F N KEND+ H A+ + D Sbjct: 69 LVQFTSLDHLL---NR-LQNEGYYGGVRLLMAICKVFRNYCKENDIQLHQANFSLSYD 122 >At4g30710.2 68417.m04353 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 28.7 bits (61), Expect = 3.8 Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 6/72 (8%) Frame = -2 Query: 355 ETAIHVSAPREEDTFFNIVHAYVVLVD------VGSSDIFLQVRMSHSMSAACLASFSQW 194 E+ +++ E+T FN+ HA L D +G + L++++ +S+ + S W Sbjct: 452 ESVMYIQRLTSEETLFNVWHAISELQDHVTRQRIGLQQLKLEIKL-NSLLNDQMVSLEDW 510 Query: 193 ASTLFRYFSSLV 158 A+ + SSLV Sbjct: 511 ATLERDHVSSLV 522 >At4g30710.1 68417.m04352 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 28.7 bits (61), Expect = 3.8 Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 6/72 (8%) Frame = -2 Query: 355 ETAIHVSAPREEDTFFNIVHAYVVLVD------VGSSDIFLQVRMSHSMSAACLASFSQW 194 E+ +++ E+T FN+ HA L D +G + L++++ +S+ + S W Sbjct: 452 ESVMYIQRLTSEETLFNVWHAISELQDHVTRQRIGLQQLKLEIKL-NSLLNDQMVSLEDW 510 Query: 193 ASTLFRYFSSLV 158 A+ + SSLV Sbjct: 511 ATLERDHVSSLV 522 >At3g51850.1 68416.m05686 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Arabidopsis thaliana] gi|836942|gb|AAA67655; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 528 Score = 28.7 bits (61), Expect = 3.8 Identities = 13/39 (33%), Positives = 21/39 (53%) Frame = +2 Query: 386 KGQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTG 502 KG DY E + ++ +V +E+ +K +FD DG G Sbjct: 408 KGTLDYGEFVAVSLH-LQKVANDEHLRKAFSYFDKDGNG 445 >At1g35670.1 68414.m04435 calcium-dependent protein kinase 2 (CDPK2) identical to calcium-dependent protein kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 495 Score = 28.7 bits (61), Expect = 3.8 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E ++ +++EREEN +FD DG+G T Sbjct: 381 GTIDYGEFLAATLH-MNKMEREENLVAAFSYFDKDGSGYIT 420 >At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDPK9) identical to calcium-dependent protein kinase [Arabidopsis thaliana] gi|836938|gb|AAA67653; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 490 Score = 28.3 bits (60), Expect = 5.1 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E I+ +++EREEN FFD D +G T Sbjct: 377 GTIDYGEFLAATIH-LNKLEREENLVAAFSFFDKDASGYIT 416 >At4g35310.1 68417.m05019 calcium-dependent protein kinase, putative / CDPK, putative similar to calmodulin-domain protein kinase CDPK isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 556 Score = 28.3 bits (60), Expect = 5.1 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E I+ +++EREE+ +FD DG+G T Sbjct: 452 GTIDYSEFIAATIH-LNKLEREEHLVAAFQYFDKDGSGFIT 491 >At4g09570.1 68417.m01575 calcium-dependent protein kinase, putative / CDPK, putative similar to calcium-dependent protein kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 501 Score = 28.3 bits (60), Expect = 5.1 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E ++ +++EREEN +FD DG+G T Sbjct: 380 GTIDYGEFLAATLH-INKMEREENLVVAFSYFDKDGSGYIT 419 >At2g17290.1 68415.m01997 calcium-dependent protein kinase isoform 6 (CPK6) identical to calmodulin-domain protein kinase CDPK isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 544 Score = 28.3 bits (60), Expect = 5.1 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E I+ +++EREE+ +FD DG+G T Sbjct: 440 GTIDYSEFIAATIH-LNKLEREEHLVSAFQYFDKDGSGYIT 479 >At1g18890.1 68414.m02351 calcium-dependent protein kinase 1 (CDPK1) identical to calcium-dependent protein kinase [Arabidopsis thaliana] gi|604880|dbj|BAA04829; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 545 Score = 28.3 bits (60), Expect = 5.1 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGT 499 G DY E ++I+ ++E +E K MFFD DG+ Sbjct: 418 GFLDYGEFVAVIIH-LQKIENDELFKLAFMFFDKDGS 453 >At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing protein contains Pfam profile PF01465: GRIP domain; supporting cDNA gi|20303028|gb|AF499634.1| Length = 765 Score = 27.9 bits (59), Expect = 6.7 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = +3 Query: 105 ENRLKENDVDFHPADLERTRDEKYLKRVLAHCEKDAKQAADMLWD 239 E LKE + + + ER R ++ L+ LA EK+ ++ A L D Sbjct: 462 EEALKEAEKEVYLVSAERDRAQQDLQSALASLEKELEERAGALKD 506 >At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing protein contains Pfam profile PF01465: GRIP domain; supporting cDNA gi|20303028|gb|AF499634.1| Length = 788 Score = 27.9 bits (59), Expect = 6.7 Identities = 15/45 (33%), Positives = 24/45 (53%) Frame = +3 Query: 105 ENRLKENDVDFHPADLERTRDEKYLKRVLAHCEKDAKQAADMLWD 239 E LKE + + + ER R ++ L+ LA EK+ ++ A L D Sbjct: 462 EEALKEAEKEVYLVSAERDRAQQDLQSALASLEKELEERAGALKD 506 >At5g60770.1 68418.m07625 high-affinity nitrate transporter, putative strong similarity to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI:3608362 Length = 527 Score = 27.9 bits (59), Expect = 6.7 Identities = 15/47 (31%), Positives = 20/47 (42%), Gaps = 1/47 (2%) Frame = -2 Query: 274 VGSSDIFLQVRMSHSMSAACLASFSQWASTLFR-YFSSLVRSKSAGW 137 VG + ++ VR A S W ST+F LV +AGW Sbjct: 148 VGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIGLVNGTAAGW 194 >At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein similar to polyphosphoinositide binding protein Ssh2p (GI:2739046) {Glycine max};; contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus Length = 409 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/47 (29%), Positives = 23/47 (48%) Frame = +1 Query: 535 LITLFKCYYPYFLNYIIIYNMPWVLSAAFKVVKTLLPAKAVEKMKFV 675 +++LF+ YP + I N+PW S + + L + K KFV Sbjct: 218 ILSLFQDNYPELVATKIFINVPWYFSVIYSMFSPFLTQRT--KSKFV 262 >At5g58410.1 68418.m07314 expressed protein contains similarity to hypothetical proteins Length = 1873 Score = 27.5 bits (58), Expect = 8.9 Identities = 14/39 (35%), Positives = 21/39 (53%) Frame = -1 Query: 134 VYIVFFETILKNLRTSAAVAMITDKTIISLKTTGYCLEL 18 ++I+F E + AA MITDK I +T+ CL + Sbjct: 1130 LHILFKEDLWGEEEAMAAFKMITDKLFIGEETSKNCLRI 1168 >At4g38230.1 68417.m05399 calcium-dependent protein kinase, putative / CDPK, putative calmodulin-domain protein kinase CDPK isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 340 Score = 27.5 bits (58), Expect = 8.9 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +2 Query: 389 GQRDYDELKKIMIYWFDRVEREENGKKISMFFDMDGTGLST 511 G DY E I+ +++EREE+ +FD DG+G T Sbjct: 235 GTIDYGEFIAATIH-LNKLEREEHLLSAFRYFDKDGSGYIT 274 >At1g78090.1 68414.m09100 trehalose-6-phosphate phosphatase (TPPB) identical to trehalose-6-phosphate phosphatase (AtTPPB) GI:2944180 [Arabidopsis thaliana] Length = 374 Score = 27.5 bits (58), Expect = 8.9 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +2 Query: 434 FDRVEREENGKKISMFFDMDGT 499 FD + GK+I MF D DGT Sbjct: 106 FDEIVNAAKGKQIVMFLDYDGT 127 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,221,823 Number of Sequences: 28952 Number of extensions: 367785 Number of successful extensions: 936 Number of sequences better than 10.0: 32 Number of HSP's better than 10.0 without gapping: 913 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 936 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1467502800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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