Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20626
(702 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 182 4e-48
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 178 5e-47
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 164 6e-43
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 135 4e-34
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.8
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.7
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.6
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 182 bits (442), Expect = 4e-48
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = +3
Query: 255 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 434
GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 109
Query: 435 DCAVLIVAAGTGEFEAGISKNGQTR 509
DCAVLIVAAGTGEFEAGISKNGQTR
Sbjct: 110 DCAVLIVAAGTGEFEAGISKNGQTR 134
Score = 135 bits (326), Expect = 4e-34
Identities = 63/64 (98%), Positives = 63/64 (98%)
Frame = +2
Query: 509 EHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 688
EHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPIS
Sbjct: 135 EHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 194
Query: 689 GWHG 700
GWHG
Sbjct: 195 GWHG 198
Score = 105 bits (253), Expect = 3e-25
Identities = 49/51 (96%), Positives = 49/51 (96%)
Frame = +1
Query: 109 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMVK 261
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 178 bits (433), Expect = 5e-47
Identities = 83/85 (97%), Positives = 84/85 (98%)
Frame = +3
Query: 255 GKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQA 434
GKGSFKYAWVLDKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQA
Sbjct: 50 GKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQA 109
Query: 435 DCAVLIVAAGTGEFEAGISKNGQTR 509
DCAVLIVAAG GEFEAGISKNGQTR
Sbjct: 110 DCAVLIVAAGIGEFEAGISKNGQTR 134
Score = 127 bits (307), Expect = 9e-32
Identities = 59/64 (92%), Positives = 61/64 (95%)
Frame = +2
Query: 509 EHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 688
EHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIGYN A+VAFVPIS
Sbjct: 135 EHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPIS 194
Query: 689 GWHG 700
GWHG
Sbjct: 195 GWHG 198
Score = 105 bits (253), Expect = 3e-25
Identities = 49/51 (96%), Positives = 49/51 (96%)
Frame = +1
Query: 109 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMVK 261
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEM K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 164 bits (399), Expect = 6e-43
Identities = 77/77 (100%), Positives = 77/77 (100%)
Frame = +3
Query: 279 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 458
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 459 AGTGEFEAGISKNGQTR 509
AGTGEFEAGISKNGQTR
Sbjct: 61 AGTGEFEAGISKNGQTR 77
Score = 135 bits (326), Expect = 4e-34
Identities = 63/64 (98%), Positives = 63/64 (98%)
Frame = +2
Query: 509 EHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 688
EHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPIS
Sbjct: 78 EHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 137
Query: 689 GWHG 700
GWHG
Sbjct: 138 GWHG 141
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 135 bits (326), Expect = 4e-34
Identities = 63/64 (98%), Positives = 63/64 (98%)
Frame = +2
Query: 509 EHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 688
EHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPIS
Sbjct: 62 EHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 121
Query: 689 GWHG 700
GWHG
Sbjct: 122 GWHG 125
Score = 131 bits (317), Expect = 5e-33
Identities = 61/61 (100%), Positives = 61/61 (100%)
Frame = +3
Query: 327 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 506
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 507 R 509
R
Sbjct: 61 R 61
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 366 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 470
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.53
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 121 KTHINIVVIGHVDSGKST 174
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 130 INIVVIGHVDSGKST 174
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 351 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 452
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.7
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 351 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 452
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 8.6
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +3
Query: 570 WIPLNHHTVSPDLRKSRRKY 629
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 205,674
Number of Sequences: 438
Number of extensions: 4061
Number of successful extensions: 18
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21561255
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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