Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20623
         (562 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...   127   1e-30
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...   121   8e-29
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...    84   1e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...    84   1e-17
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    47   2e-06
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.35 
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    29   0.62 
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi...    27   1.9  
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    26   3.3  
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo...    26   4.4  
SPAC2F7.07c |||histone deacetylase complex subunit Rco1 |Schizos...    26   4.4  
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi...    25   7.6  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score =  127 bits (307), Expect = 1e-30
 Identities = 58/83 (69%), Positives = 70/83 (84%)
 Frame = -2

Query: 249 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRK 70
           AP    +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRK
Sbjct: 8   APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRK 67

Query: 69  SEIEYYALLAKTGVHHYSGNNIE 1
           SE+EYYA+L++  VHHYSGNNI+
Sbjct: 68  SELEYYAMLSRCSVHHYSGNNID 90


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score =  121 bits (291), Expect = 8e-29
 Identities = 54/79 (68%), Positives = 66/79 (83%)
 Frame = -2

Query: 237 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 58
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63

Query: 57  YYALLAKTGVHHYSGNNIE 1
           YYA+L+K  VHHY+G NI+
Sbjct: 64  YYAMLSKANVHHYAGTNID 82


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score = 84.2 bits (199), Expect = 1e-17
 Identities = 37/40 (92%), Positives = 39/40 (97%)
 Frame = +1

Query: 325 AKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 444
           +KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 404 SKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 443


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score = 84.2 bits (199), Expect = 1e-17
 Identities = 37/40 (92%), Positives = 39/40 (97%)
 Frame = +1

Query: 325 AKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDS 444
           +KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS
Sbjct: 400 SKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDS 439


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 20/41 (48%), Positives = 29/41 (70%), Gaps = 3/41 (7%)
 Frame = +1

Query: 328 KRAFVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVGMD 441
           ++AF+HWY GEGM+E EF+EA     DL +  + Y+E G+D
Sbjct: 391 RKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGID 431


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.35
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = -2

Query: 225 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 100
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 28.7 bits (61), Expect = 0.62
 Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 8/69 (11%)
 Frame = +1

Query: 250 VKLMNPRAQMEKKRLKTQ-MK---EVDLEAKRAFVHWYVGEGM----EEGEFSEAREDLA 405
           V + +P  ++E  R+ T+ MK   +VDLE   A  H YVG  +     E    + RE + 
Sbjct: 389 VGIPDPTGRLEILRIHTKNMKLADDVDLEQIAAETHGYVGSDLASLCSEAAMQQIREKMD 448

Query: 406 ALEKDYEEV 432
            ++ D +E+
Sbjct: 449 MIDLDEDEI 457


>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
            homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 3071

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = +1

Query: 214  LFLFLCCNHFGSVKLMNPRAQME-KKRLKTQMKEVDLEAK 330
            +FL+ C  HF +V L +    ME +++   Q +  DLE K
Sbjct: 1049 IFLYDCDQHFSTVCLYSANMHMEFREKFFLQARFYDLEVK 1088


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 17/58 (29%), Positives = 32/58 (55%)
 Frame = -2

Query: 249 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 76
           APK +  KK  KT++  + +  ++        G K+ +K +R+G+  LVI+A +  P+
Sbjct: 33  APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82


>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
           component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 536

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 15/60 (25%), Positives = 30/60 (50%)
 Frame = +1

Query: 253 KLMNPRAQMEKKRLKTQMKEVDLEAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 432
           +L   + Q+EKK+ +     + LE +   V   + +   + +FSE    L++L   +EE+
Sbjct: 462 ELEKKKEQVEKKQEELMQTRIVLEEQVFLVENMIEDAKAKRKFSEVETLLSSLAPLHEEI 521


>SPAC2F7.07c |||histone deacetylase complex subunit Rco1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 607

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +3

Query: 249 RKANESKSTNGKEAAQNSNERGRS 320
           R A+ +K+T+ +  AQN N  GR+
Sbjct: 43  RSASPNKATSRRNRAQNGNSNGRA 66


>SPAC977.14c |||aldo/keto reductase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 351

 Score = 25.0 bits (52), Expect = 7.6
 Identities = 11/31 (35%), Positives = 20/31 (64%)
 Frame = -2

Query: 117 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVH 25
           ++ +VI++K   P+RK  I+ +  L+  GVH
Sbjct: 87  RSSIVILSKCFFPVRKDLIKIFGDLSSRGVH 117


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,192,890
Number of Sequences: 5004
Number of extensions: 44155
Number of successful extensions: 147
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 236012634
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)