BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20620 (726 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 154 5e-38 At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 102 3e-22 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 96 2e-20 At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 70 2e-12 At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 67 1e-11 At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 40 0.001 At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 40 0.001 At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 38 0.007 At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 38 0.009 At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 34 0.083 At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 34 0.11 At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 34 0.11 At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.25 At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.25 At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.25 At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 31 0.59 At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 31 0.59 At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 31 0.78 At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 31 0.78 At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 1.0 At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.4 At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.4 At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.4 At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 29 4.1 At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 29 4.1 At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc... 29 4.1 At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 28 5.5 At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.5 At1g22730.1 68414.m02840 MA3 domain-containing protein contains ... 28 5.5 At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 5.5 At5g62310.1 68418.m07822 incomplete root hair elongation (IRE) /... 28 7.2 At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 7.2 At5g02010.1 68418.m00120 expressed protein contains Pfam profile... 28 7.2 At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nic... 27 9.6 At5g06810.1 68418.m00770 mitochondrial transcription termination... 27 9.6 At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 27 9.6 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 154 bits (374), Expect = 5e-38 Identities = 72/85 (84%), Positives = 78/85 (91%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QTREHALLAFTLGVKQ+I NKMD Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156 Score = 136 bits (328), Expect = 2e-32 Identities = 64/71 (90%), Positives = 65/71 (91%) Frame = +1 Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 223 DKLKAERERGI 255 DKLKAERERGI Sbjct: 61 DKLKAERERGI 71 Score = 85.4 bits (202), Expect = 3e-17 Identities = 35/57 (61%), Positives = 46/57 (80%) Frame = +2 Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 679 +T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 154 bits (374), Expect = 5e-38 Identities = 72/85 (84%), Positives = 78/85 (91%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QTREHALLAFTLGVKQ+I NKMD Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156 Score = 136 bits (328), Expect = 2e-32 Identities = 64/71 (90%), Positives = 65/71 (91%) Frame = +1 Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 223 DKLKAERERGI 255 DKLKAERERGI Sbjct: 61 DKLKAERERGI 71 Score = 85.4 bits (202), Expect = 3e-17 Identities = 35/57 (61%), Positives = 46/57 (80%) Frame = +2 Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 679 +T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 154 bits (374), Expect = 5e-38 Identities = 72/85 (84%), Positives = 78/85 (91%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QTREHALLAFTLGVKQ+I NKMD Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156 Score = 136 bits (328), Expect = 2e-32 Identities = 64/71 (90%), Positives = 65/71 (91%) Frame = +1 Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 223 DKLKAERERGI 255 DKLKAERERGI Sbjct: 61 DKLKAERERGI 71 Score = 85.4 bits (202), Expect = 3e-17 Identities = 35/57 (61%), Positives = 46/57 (80%) Frame = +2 Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 679 +T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 154 bits (374), Expect = 5e-38 Identities = 72/85 (84%), Positives = 78/85 (91%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+G Sbjct: 72 TIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDG 131 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QTREHALLAFTLGVKQ+I NKMD Sbjct: 132 QTREHALLAFTLGVKQMICCCNKMD 156 Score = 136 bits (328), Expect = 2e-32 Identities = 64/71 (90%), Positives = 65/71 (91%) Frame = +1 Query: 43 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 222 MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60 Query: 223 DKLKAERERGI 255 DKLKAERERGI Sbjct: 61 DKLKAERERGI 71 Score = 85.4 bits (202), Expect = 3e-17 Identities = 35/57 (61%), Positives = 46/57 (80%) Frame = +2 Query: 509 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKG 679 +T P YS+ R++EI KEVSSY+KK+GYNP + FVPISG+ GDNM+E ST + W+KG Sbjct: 157 ATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 102 bits (244), Expect = 3e-22 Identities = 47/85 (55%), Positives = 60/85 (70%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G + G Sbjct: 166 TVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYERGG 225 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QTREH LA TLGV +LIV VNKMD Sbjct: 226 QTREHVQLAKTLGVSKLIVVVNKMD 250 Score = 70.1 bits (164), Expect = 1e-12 Identities = 29/67 (43%), Positives = 48/67 (71%) Frame = +1 Query: 52 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 231 +K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157 Query: 232 KAERERG 252 + ER +G Sbjct: 158 EEERLKG 164 Score = 39.1 bits (87), Expect = 0.003 Identities = 20/55 (36%), Positives = 34/55 (61%), Gaps = 3/55 (5%) Frame = +2 Query: 524 YSEPRFEEIKKEVSSYIKKIGYNPAA-VAFVPISGWHGDNMLEPSTK--MPWFKG 679 +S+ R++EI++++ ++K GYN V F+PISG G NM + + PW+ G Sbjct: 256 WSKERYDEIEQKMVPFLKASGYNTKKDVVFLPISGLMGKNMDQRMGQEICPWWSG 310 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 95.9 bits (228), Expect = 2e-20 Identities = 44/99 (44%), Positives = 66/99 (66%), Gaps = 1/99 (1%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK-N 431 T+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG Sbjct: 304 TMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDNLK 363 Query: 432 GQTREHALLAFTLGVKQLIVGVNKMDPLNHHTVSPDLRK 548 GQTREHA + GV+Q+IV +NKMD + + DL K Sbjct: 364 GQTREHARVLRGFGVEQVIVAINKMDIVGYSKERFDLIK 402 Score = 83.4 bits (197), Expect = 1e-16 Identities = 35/66 (53%), Positives = 49/66 (74%) Frame = +1 Query: 58 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 237 + +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+ Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297 Query: 238 ERERGI 255 ERERGI Sbjct: 298 ERERGI 303 Score = 34.7 bits (76), Expect = 0.063 Identities = 15/55 (27%), Positives = 32/55 (58%), Gaps = 3/55 (5%) Frame = +2 Query: 524 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML-EPSTK--MPWFKG 679 YS+ RF+ IK+ V S+++ + +++ ++P+S N++ PS W++G Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447 >At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) identical to SWISS-PROT:P17745 elongation factor Tu, chloroplast precursor (EF-Tu) [Arabidopsis thaliana] Length = 476 Score = 69.7 bits (163), Expect = 2e-12 Identities = 35/88 (39%), Positives = 53/88 (60%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G Sbjct: 129 TINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP------- 181 Query: 435 QTREHALLAFTLGVKQLIVGVNKMDPLN 518 QT+EH LLA +GV ++V +NK D ++ Sbjct: 182 QTKEHILLAKQVGVPDMVVFLNKEDQVD 209 Score = 39.1 bits (87), Expect = 0.003 Identities = 17/45 (37%), Positives = 25/45 (55%) Frame = +1 Query: 40 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 174 K ++K H+NI IGHVD GK+T T L I +K+++ Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116 >At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu, putative similar to mitochondrial elongation factor Tu [Arabidopsis thaliana] gi|1149571|emb|CAA61511 Length = 454 Score = 67.3 bits (157), Expect = 1e-11 Identities = 35/85 (41%), Positives = 51/85 (60%) Frame = +3 Query: 255 TIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNG 434 TI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G Sbjct: 117 TIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP------- 169 Query: 435 QTREHALLAFTLGVKQLIVGVNKMD 509 QT+EH LLA +GV L+ +NK+D Sbjct: 170 QTKEHILLARQVGVPSLVCFLNKVD 194 Score = 32.7 bits (71), Expect = 0.25 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = +1 Query: 49 KEKTHINIVVIGHVDSGKSTTT 114 + K H+N+ IGHVD GK+T T Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84 >At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 471 Score = 40.3 bits (90), Expect = 0.001 Identities = 23/78 (29%), Positives = 39/78 (50%) Frame = +3 Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 464 T + +V+ +D PGH + M+ G + D A+L++AA QT EH Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177 Query: 465 TLGVKQLIVGVNKMDPLN 518 + +K +I+ NK+D +N Sbjct: 178 MMRLKHIIILQNKIDLIN 195 >At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to SP|Q09130 Eukaryotic translation initiation factor 2 gamma subunit (eIF-2- gamma) {Schizosaccharomyces pombe}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain; isoform predicted to contain a TG non-consensus acceptor splice site. Length = 284 Score = 40.3 bits (90), Expect = 0.001 Identities = 23/78 (29%), Positives = 39/78 (50%) Frame = +3 Query: 285 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 464 T + +V+ +D PGH + M+ G + D A+L++AA QT EH Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177 Query: 465 TLGVKQLIVGVNKMDPLN 518 + +K +I+ NK+D +N Sbjct: 178 MMRLKHIIILQNKIDLIN 195 >At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative similar to gb|U37354 from S. pombe. ESTs gb|T41979, gb|N37284 and gb|N37529 come from this gene Length = 465 Score = 37.9 bits (84), Expect = 0.007 Identities = 25/81 (30%), Positives = 39/81 (48%), Gaps = 4/81 (4%) Frame = +3 Query: 279 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 446 FE SK +V+ +D PGH + M+ G + D A+L++AA QT E Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165 Query: 447 HALLAFTLGVKQLIVGVNKMD 509 H + +K +I+ NK+D Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186 >At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2 subunit 3, putative / eIF2S3, putative / eIF-2-gamma, putative Length = 465 Score = 37.5 bits (83), Expect = 0.009 Identities = 22/71 (30%), Positives = 35/71 (49%) Frame = +3 Query: 297 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 476 +V+ +D PGH + M+ G + D A+LI+AA QT EH + + Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173 Query: 477 KQLIVGVNKMD 509 K +I+ NK+D Sbjct: 174 KDIIIIQNKID 184 >At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-binding protein GUF1 - Saccharomyces cerevisiae, PIR:S50374 Length = 661 Score = 34.3 bits (75), Expect = 0.083 Identities = 28/93 (30%), Positives = 42/93 (45%) Frame = +3 Query: 282 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLA 461 E S Y + +ID PGH DF + S A+L+V A G QT + LA Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG-------VQAQTVANFYLA 183 Query: 462 FTLGVKQLIVGVNKMDPLNHHTVSPDLRKSRRK 560 F + ++ +NK+D T P+ K++ K Sbjct: 184 FEANL-TIVPVINKID---QPTADPERVKAQLK 212 Score = 27.9 bits (59), Expect = 7.2 Identities = 16/40 (40%), Positives = 19/40 (47%) Frame = +1 Query: 31 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 150 D K EK N +I H+D GKST L+ G I K Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95 >At1g06220.2 68414.m00656 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 33.9 bits (74), Expect = 0.11 Identities = 18/49 (36%), Positives = 23/49 (46%) Frame = +3 Query: 258 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404 + + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At1g06220.1 68414.m00655 elongation factor Tu family protein similar to Cryptosporidium parvum elongation factor-2 GB:U21667 GI:706974 from [Cryptosporidium parvum] Length = 987 Score = 33.9 bits (74), Expect = 0.11 Identities = 18/49 (36%), Positives = 23/49 (46%) Frame = +3 Query: 258 IDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404 + + L + Y I+D PGH +F M AD AVLIV A G Sbjct: 197 MSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245 >At4g11160.1 68417.m01808 translation initiation factor IF-2, mitochondrial, putative similar to SP|P46198|IF2M_BOVIN Translation initiation factor IF-2, mitochondrial precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus} Length = 743 Score = 32.7 bits (71), Expect = 0.25 Identities = 14/35 (40%), Positives = 19/35 (54%) Frame = +3 Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404 +T +D PGH F + G + D VL+VAA G Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304 >At3g22980.1 68416.m02898 elongation factor Tu family protein similar to eukaryotic translation elongation factor 2 GB:NP_001952 [Homo sapiens] Length = 1015 Score = 32.7 bits (71), Expect = 0.25 Identities = 13/25 (52%), Positives = 16/25 (64%) Frame = +1 Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGG 141 NI ++ HVD GK+T HLI GG Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35 Score = 32.7 bits (71), Expect = 0.25 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +3 Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404 Y + +ID+PGH DF + T +D A+++V A G Sbjct: 74 YSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110 >At1g62750.1 68414.m07082 elongation factor Tu family protein similar to elongation factor G SP:P34811 [Glycine max (Soybean)] Length = 783 Score = 32.7 bits (71), Expect = 0.25 Identities = 12/28 (42%), Positives = 20/28 (71%) Frame = +1 Query: 55 KTHINIVVIGHVDSGKSTTTGHLIYKCG 138 K + NI ++ H+D+GK+TTT ++Y G Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121 >At2g45030.1 68415.m05606 mitochondrial elongation factor, putative similar to SP|P25039 Elongation factor G 1, mitochondrial precursor (mEF-G-1) {Saccharomyces cerevisiae}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03764: Elongation factor G domain IV, PF00679: Elongation factor G C-terminus Length = 754 Score = 31.5 bits (68), Expect = 0.59 Identities = 17/52 (32%), Positives = 25/52 (48%) Frame = +3 Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 449 Y V IID PGH DF + D A+L++ + G I+ + Q R + Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184 Score = 29.1 bits (62), Expect = 3.1 Identities = 21/63 (33%), Positives = 33/63 (52%) Frame = +1 Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 246 NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 247 RGI 255 +GI Sbjct: 117 KGI 119 >At1g45332.1 68414.m05195 mitochondrial elongation factor, putative similar to mitochondrial elongation factor GI:3917 from [Saccharomyces cerevisiae] Length = 754 Score = 31.5 bits (68), Expect = 0.59 Identities = 17/52 (32%), Positives = 25/52 (48%) Frame = +3 Query: 294 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREH 449 Y V IID PGH DF + D A+L++ + G I+ + Q R + Sbjct: 133 YKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRY 184 Score = 29.1 bits (62), Expect = 3.1 Identities = 21/63 (33%), Positives = 33/63 (52%) Frame = +1 Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 246 NI + H+DSGK+T T +++ G R E E ++ G G+ +D + ERE Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTG----RIHEIHEVRGRD-GVGA-----KMDSMDLERE 116 Query: 247 RGI 255 +GI Sbjct: 117 KGI 119 >At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1201 Score = 31.1 bits (67), Expect = 0.78 Identities = 22/70 (31%), Positives = 35/70 (50%) Frame = +3 Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485 +ID PGH F G+S D A+L+V + + G+ QT E +L + + Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756 Query: 486 IVGVNKMDPL 515 I+ +NK+D L Sbjct: 757 IIALNKVDRL 766 >At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profiles PF00009: Elongation factor Tu GTP binding domain, PF03144: Elongation factor Tu domain 2 Length = 1088 Score = 31.1 bits (67), Expect = 0.78 Identities = 23/72 (31%), Positives = 34/72 (47%) Frame = +3 Query: 300 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 479 + +ID PGH F G++ D A+L+V + G+ QT E L VK Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610 Query: 480 QLIVGVNKMDPL 515 I+ +NK+D L Sbjct: 611 -FIIALNKVDRL 621 >At5g08650.1 68418.m01029 GTP-binding protein LepA, putative Length = 681 Score = 30.7 bits (66), Expect = 1.0 Identities = 13/42 (30%), Positives = 23/42 (54%) Frame = +3 Query: 279 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 404 +E + + + +ID PGH DF + + + A+L+V A G Sbjct: 147 YEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188 Score = 30.3 bits (65), Expect = 1.4 Identities = 37/124 (29%), Positives = 50/124 (40%), Gaps = 1/124 (0%) Frame = +1 Query: 67 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 246 N +I H+D GKST L+ G + R + K F LD + ERE Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131 Query: 247 RGIQSILLSGSSKLASTMLP-SLMLLDTEISSRT*SQEPLRLIALCSS*LPVPVNSKLVS 423 RGI L + + P L L+DT S E R +A C L V S+ V Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTP-GHVDFSYEVSRSLAACEGALLVVDASQGVE 190 Query: 424 LRTV 435 +T+ Sbjct: 191 AQTL 194 >At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to SP|O60841 Translation initiation factor IF-2 {Homo sapiens}; contains Pfam profile PF00009: Elongation factor Tu GTP binding domain Length = 630 Score = 30.3 bits (65), Expect = 1.4 Identities = 22/70 (31%), Positives = 34/70 (48%) Frame = +3 Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485 +ID PGH F G+S D A+L+V + G+ QT E +L + + Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DITHGLQP--QTIE-SLNLLRMRNTEF 164 Query: 486 IVGVNKMDPL 515 I+ +NK+D L Sbjct: 165 IIALNKVDRL 174 >At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2 family protein / eIF-2 family protein similar to IF2 protein [Drosophila melanogaster] GI:7108770; contains Pfam profile PF03144: Elongation factor Tu domain 2 Length = 1294 Score = 30.3 bits (65), Expect = 1.4 Identities = 23/70 (32%), Positives = 34/70 (48%) Frame = +3 Query: 306 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 485 +ID PGH F G+S D A+L+V + G+ QT E +L + + Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIMHGLEP--QTIE-SLNLLRMRNTEF 823 Query: 486 IVGVNKMDPL 515 IV +NK+D L Sbjct: 824 IVALNKVDRL 833 >At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, putative similar to ELONGATION FACTOR 2 GB:O14460 from [Schizosaccharomyces pombe] Length = 843 Score = 29.5 bits (63), Expect = 2.4 Identities = 11/34 (32%), Positives = 20/34 (58%) Frame = +3 Query: 288 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 389 ++Y + +ID+PGH DF + D A+++V Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129 Score = 29.1 bits (62), Expect = 3.1 Identities = 13/24 (54%), Positives = 15/24 (62%) Frame = +1 Query: 67 NIVVIGHVDSGKSTTTGHLIYKCG 138 N+ VI HVD GKST T L+ G Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44 >At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329: Protein of unknown function (DUF731) Length = 442 Score = 28.7 bits (61), Expect = 4.1 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = +2 Query: 503 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 622 N +TE + P+ E K E+S++I IG+ V F P S Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100 >At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329: Protein of unknown function (DUF731) Length = 440 Score = 28.7 bits (61), Expect = 4.1 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = +2 Query: 503 NGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 622 N +TE + P+ E K E+S++I IG+ V F P S Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100 >At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar identical to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis thaliana]; supported by full-length cDNA GI:14517549; identical to cDNA Beta-fructosidase GI:3115854 Length = 648 Score = 28.7 bits (61), Expect = 4.1 Identities = 17/54 (31%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = -2 Query: 539 IWAHCMVVQWIHFVYSYDELFDTEGESEQGMLTG-LTVLRDTSFEFTGTGSYDE 381 +W H + IH++Y + + G+ TG T L D S TGS DE Sbjct: 148 VWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVMLYTGSTDE 201 >At5g44000.1 68418.m05384 glutathione S-transferase C-terminal domain-containing protein contains Pfam domain PF00043: Glutathione S-transferase, C-terminal domain Length = 399 Score = 28.3 bits (60), Expect = 5.5 Identities = 12/23 (52%), Positives = 13/23 (56%) Frame = -3 Query: 691 LHLPSFEPRHFG*RLQHVVSVPS 623 L PSF PRHF R+ H PS Sbjct: 9 LTFPSFSPRHFSPRMSHQSPKPS 31 >At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein similar to 1,4-alpha-glucan branching enzyme [Solanum tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme (EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum tuberosum} SP|P30924; contains Pfam profiles: PF00128 Alpha amylase catalytic domain, PF02922 Isoamylase N-terminal domain Length = 777 Score = 28.3 bits (60), Expect = 5.5 Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%) Frame = +2 Query: 527 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 616 SEP+ FEE K+V ++K+ GYN + VP Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289 >At1g22730.1 68414.m02840 MA3 domain-containing protein contains Pfam profile PF02847: MA3 domain; low similarity to programmed cell death 4 protein [Gallus gallus] GI:12958564 Length = 693 Score = 28.3 bits (60), Expect = 5.5 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +3 Query: 468 LGVKQLIVGVNKMDPLNHHTVSPDLRKSRRKYP 566 L + QL + + +DPL ++ DL KSRR P Sbjct: 14 LCISQLKISSSSLDPLPQANMAEDLTKSRRHSP 46 >At1g17220.1 68414.m02098 translation initiation factor IF-2, chloroplast, putative similar to SP|P57997|IF2C_PHAVU Translation initiation factor IF-2, chloroplast precursor (PvIF2cp) {Phaseolus vulgaris} Length = 1026 Score = 28.3 bits (60), Expect = 5.5 Identities = 19/67 (28%), Positives = 29/67 (43%) Frame = +3 Query: 309 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 488 +D PGH F G D A+++VAA G QT E A+ ++ Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG-------IRPQTNE-AIAHAKAAAVPIV 608 Query: 489 VGVNKMD 509 + +NK+D Sbjct: 609 IAINKID 615 >At5g62310.1 68418.m07822 incomplete root hair elongation (IRE) / protein kinase, putative nearly identical to IRE (incomplete root hair elongation) [Arabidopsis thaliana] gi|6729346|dbj|BAA89783 Length = 1168 Score = 27.9 bits (59), Expect = 7.2 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = -2 Query: 344 VLDEISVSRSINDGNIVLASFELPESNIDCIPRSRSAFSL 225 V D +S S ++ S + +DC+PRS++ FSL Sbjct: 549 VADSARLSNSSRQEDLDEISQRCSDDMLDCVPRSQNTFSL 588 >At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) Monovalent cation:proton antiporter family 2 (CPA2 family) member, PMID:11500563; related to glutathione-regulated potassium-efflux system protein [Escherichia coli] GP|606284|gb|AAA58147 Length = 568 Score = 27.9 bits (59), Expect = 7.2 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +2 Query: 533 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 643 PRF ++ ++SS ++ Y AAVAF +S W D + Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389 >At5g02010.1 68418.m00120 expressed protein contains Pfam profile PF03759: Domain of unknown function (DUF315) Length = 546 Score = 27.9 bits (59), Expect = 7.2 Identities = 16/50 (32%), Positives = 26/50 (52%) Frame = -2 Query: 395 GSYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCIPR 246 G E+S L+ D + + +IN +I LA E+PES ++ +PR Sbjct: 237 GGLQENSRKQLQHKRDCTNQILKAAMAIN--SITLADMEIPESYLESLPR 284 >At5g64600.1 68418.m08118 expressed protein similar to axi 1 [Nicotiana tabacum] GI:559921; contains Pfam profile PF03138: Plant protein family Length = 522 Score = 27.5 bits (58), Expect = 9.6 Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%) Frame = +2 Query: 470 RCQTAHRRSKQNGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHG--DNM 643 R T+H + K STE E KEV ++K +GY+ + V ++ +G D + Sbjct: 313 RESTSHWKIKSINSTEQR-EEGLCPLTPKEVGIFLKGLGYSQSTVIYIAAGEIYGGDDRL 371 Query: 644 LEPSTKMP 667 E ++ P Sbjct: 372 SELKSRFP 379 >At5g06810.1 68418.m00770 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 1141 Score = 27.5 bits (58), Expect = 9.6 Identities = 16/52 (30%), Positives = 27/52 (51%) Frame = +1 Query: 118 HLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGIQSILLS 273 H + G+D + I K KEA+E+ S++ + K+KA + G + LS Sbjct: 174 HHVLCYSGVDPKRIGKIFKEAREV--FSYETGVLASKIKAYEDLGFSRLFLS 223 >At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha subunit, chloroplast / 60 kDa chaperonin alpha subunit / CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO subunit binding-protein alpha subunit, chloroplast precursor (60 kDa chaperonin alpha subunit, CPN-60 alpha) [Arabidopsis thaliana] Length = 586 Score = 27.5 bits (58), Expect = 9.6 Identities = 16/58 (27%), Positives = 29/58 (50%) Frame = -2 Query: 392 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCIPRSRSAFSLSN 219 ++D+HS +L+ D + D + ++ N+VL F P+ D + +R A L N Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,653,131 Number of Sequences: 28952 Number of extensions: 359886 Number of successful extensions: 1159 Number of sequences better than 10.0: 39 Number of HSP's better than 10.0 without gapping: 1088 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1152 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1584903024 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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