BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20618 (715 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro... 144 8e-36 SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch... 144 8e-36 SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos... 143 2e-35 SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 30 0.38 SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po... 29 0.50 SPAC2E12.02 |hsf1|hstf, hsf|transcription factor Hsf1|Schizosacc... 28 1.2 SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 27 2.0 SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 27 3.5 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 3.5 SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharom... 26 6.1 SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha... 26 6.1 SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyce... 26 6.1 SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 25 8.1 >SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 144 bits (350), Expect = 8e-36 Identities = 64/83 (77%), Positives = 74/83 (89%) Frame = +1 Query: 256 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 435 MDV+S+EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRT Sbjct: 87 MDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRT 146 Query: 436 IRYPDPLIKVNDSIQLDIATTKI 504 IRYPDPLIKVND+I+L++ T KI Sbjct: 147 IRYPDPLIKVNDTIKLNLETNKI 169 Score = 140 bits (340), Expect = 1e-34 Identities = 62/83 (74%), Positives = 69/83 (83%) Frame = +2 Query: 5 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 184 RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV Sbjct: 3 RGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVK 62 Query: 185 KIVKQRLIKVDGKVRTDPTYPAG 253 I+ QRLIKVDGKVRTD T+P G Sbjct: 63 AILMQRLIKVDGKVRTDSTFPTG 85 Score = 95.9 bits (228), Expect = 5e-21 Identities = 42/68 (61%), Positives = 55/68 (80%) Frame = +3 Query: 510 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKG 689 FIKF++ M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ Sbjct: 172 FIKFDTSAQVMVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGET 231 Query: 690 TKAYISLP 713 K++ISLP Sbjct: 232 GKSWISLP 239 >SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 2|||Manual Length = 262 Score = 144 bits (350), Expect = 8e-36 Identities = 64/83 (77%), Positives = 74/83 (89%) Frame = +1 Query: 256 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 435 MDV+S+EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRT Sbjct: 87 MDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRT 146 Query: 436 IRYPDPLIKVNDSIQLDIATTKI 504 IRYPDPLIKVND+I+L++ T KI Sbjct: 147 IRYPDPLIKVNDTIKLNLETNKI 169 Score = 139 bits (336), Expect = 4e-34 Identities = 61/83 (73%), Positives = 69/83 (83%) Frame = +2 Query: 5 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 184 RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV Sbjct: 3 RGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVK 62 Query: 185 KIVKQRLIKVDGKVRTDPTYPAG 253 I+ QRLI+VDGKVRTD T+P G Sbjct: 63 AILMQRLIQVDGKVRTDSTFPTG 85 Score = 96.7 bits (230), Expect = 3e-21 Identities = 42/68 (61%), Positives = 55/68 (80%) Frame = +3 Query: 510 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKG 689 FIKF++ M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ Sbjct: 172 FIKFDTSAQVMVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGEA 231 Query: 690 TKAYISLP 713 K++ISLP Sbjct: 232 GKSWISLP 239 >SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizosaccharomyces pombe|chr 1|||Manual Length = 262 Score = 143 bits (347), Expect = 2e-35 Identities = 63/83 (75%), Positives = 74/83 (89%) Frame = +1 Query: 256 MDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRT 435 MDV+S++KT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKRV G K VP+LVTHDGRT Sbjct: 87 MDVISVDKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKRVQLGAKGVPFLVTHDGRT 146 Query: 436 IRYPDPLIKVNDSIQLDIATTKI 504 IRYPDPLIKVND+I+L++ T KI Sbjct: 147 IRYPDPLIKVNDTIKLNLETNKI 169 Score = 140 bits (340), Expect = 1e-34 Identities = 62/83 (74%), Positives = 69/83 (83%) Frame = +2 Query: 5 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 184 RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G EV Sbjct: 3 RGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGREVK 62 Query: 185 KIVKQRLIKVDGKVRTDPTYPAG 253 I+ QRLIKVDGKVRTD T+P G Sbjct: 63 AILMQRLIKVDGKVRTDSTFPTG 85 Score = 95.9 bits (228), Expect = 5e-21 Identities = 42/68 (61%), Positives = 55/68 (80%) Frame = +3 Query: 510 FIKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKG 689 FIKF++ M+TGGRN+GRVGTIV RE H GSF+I+H+KD+ FATRL+NVF+IG+ Sbjct: 172 FIKFDTSAQVMVTGGRNMGRVGTIVHREHHLGSFEIIHVKDALDREFATRLSNVFVIGET 231 Query: 690 TKAYISLP 713 K++ISLP Sbjct: 232 GKSWISLP 239 >SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1428 Score = 29.9 bits (64), Expect = 0.38 Identities = 10/23 (43%), Positives = 16/23 (69%) Frame = +3 Query: 459 QSQRFHPVRHCNYEDYGFIKFES 527 ++++ HPVRH YED + FE+ Sbjct: 5 ENEKIHPVRHSKYEDKSKLPFET 27 >SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces pombe|chr 2|||Manual Length = 1014 Score = 29.5 bits (63), Expect = 0.50 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 5/47 (10%) Frame = -3 Query: 704 DVRLRALAD-----YEHVVQPRGEGVSRGVLDVHNVEGAGMSLAGHD 579 + RLR+L + Y V+ P EG LD+H+ A ++L GH+ Sbjct: 887 EARLRSLFESYGKLYRVVLHPEHEGAVVEFLDIHDAGKASLALEGHE 933 >SPAC2E12.02 |hsf1|hstf, hsf|transcription factor Hsf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 609 Score = 28.3 bits (60), Expect = 1.2 Identities = 18/58 (31%), Positives = 26/58 (44%) Frame = -3 Query: 671 HVVQPRGEGVSRGVLDVHNVEGAGMSLAGHDGAHTPQVTASRDHTQVPGLKLDEAIIF 498 H P GEG R V N + + +H+P+ S+ +T PGLK + A F Sbjct: 287 HNTTPDGEGKYRSV---QNGRALNYVSSFNSDSHSPKDYISQSYTNEPGLKKESADSF 341 >SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1338 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%) Frame = -1 Query: 253 SSRISRVSPNFPIN--LYEALFH-NFQDFVSGQSILQTIPQENHQGQALAQLVGT 98 SS+ + P+ P++ +E F NFQ S L IP++ G ++A+++GT Sbjct: 27 SSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGT 81 >SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit Cct8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/35 (40%), Positives = 18/35 (51%) Frame = +3 Query: 516 KFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIV 620 KFE LC + G L R+G + E GS D+V Sbjct: 318 KFELRRLCRVVGATPLARMGVPMPEEM--GSVDVV 350 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 26.6 bits (56), Expect = 3.5 Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 1/62 (1%) Frame = +1 Query: 259 DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYL-VTHDGRT 435 +V S+EK N++F + Y+V + + + K+ ++ ++ KNVP + V H Sbjct: 1718 NVSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQIISFFLKNVPEITVQHQTEM 1777 Query: 436 IR 441 ++ Sbjct: 1778 LK 1779 >SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.8 bits (54), Expect = 6.1 Identities = 16/39 (41%), Positives = 20/39 (51%) Frame = -3 Query: 629 LDVHNVEGAGMSLAGHDGAHTPQVTASRDHTQVPGLKLD 513 LD+H V S+ D A T + AS DH VPG K + Sbjct: 174 LDLHQVAQVAQSVI--DNAAT--IAASLDHCAVPGRKFE 208 >SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccharomyces pombe|chr 1|||Manual Length = 931 Score = 25.8 bits (54), Expect = 6.1 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +2 Query: 263 LSQLRRLMNCFV*SMMLRADSQSTVSPLRRLSTSCVK 373 LS+++ L+NCF+ S + D + LR L +K Sbjct: 424 LSRIKHLLNCFLNSSTVSLDVSMVLELLRDLKKKKIK 460 >SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 398 Score = 25.8 bits (54), Expect = 6.1 Identities = 15/38 (39%), Positives = 20/38 (52%) Frame = -3 Query: 614 VEGAGMSLAGHDGAHTPQVTASRDHTQVPGLKLDEAII 501 V A LAGH VTAS+D +++ LK D A + Sbjct: 207 VHSATKYLAGHSDV-LAGVTASKDRSKILDLKADRAYL 243 >SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1261 Score = 25.4 bits (53), Expect = 8.1 Identities = 12/22 (54%), Positives = 15/22 (68%) Frame = -2 Query: 354 SLLRGDTVDCESALNIID*TKQ 289 S RGDT D +ALNI + TK+ Sbjct: 898 STRRGDTDDATTALNIFEETKR 919 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,211,187 Number of Sequences: 5004 Number of extensions: 69761 Number of successful extensions: 208 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 195 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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