Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20615
(433 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 49 2e-06
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 48 2e-06
At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase, puta... 30 0.59
At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase fa... 29 1.4
At3g63500.2 68416.m07153 expressed protein 29 1.8
At3g50180.1 68416.m05486 hypothetical protein 28 2.4
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 28 2.4
At2g44410.1 68415.m05523 expressed protein 28 3.1
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g... 27 4.1
At3g13760.1 68416.m01736 DC1 domain-containing protein contains ... 27 4.1
At4g38990.1 68417.m05524 glycosyl hydrolase family 9 protein end... 27 7.2
At2g31920.1 68415.m03899 expressed protein 27 7.2
At5g12400.1 68418.m01458 PHD finger transcription factor, putati... 26 9.6
At3g10595.1 68416.m01274 myb family transcription factor contain... 26 9.6
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 26 9.6
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 48.8 bits (111), Expect = 2e-06
Identities = 27/56 (48%), Positives = 33/56 (58%)
Frame = +3
Query: 69 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSA 236
MS K EKP L G RIKTRKR+ DP F DA+VQ GDL+ K ++S+
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIESS 55
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 48.4 bits (110), Expect = 2e-06
Identities = 28/56 (50%), Positives = 33/56 (58%)
Frame = +3
Query: 69 MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLDSA 236
MS K EKP L G RIKTRKR+ DP F DALVQ GDL+ + L+S+
Sbjct: 1 MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLESS 55
>At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase,
putative / neoxanthin cleavage enzyme, putative /
carotenoid cleavage dioxygenase, putative similar to
9-cis-epoxycarotenoid dioxygenase GI:6715257 from
[Phaseolus vulgaris]
Length = 589
Score = 30.3 bits (65), Expect = 0.59
Identities = 23/86 (26%), Positives = 41/86 (47%)
Frame = -2
Query: 423 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPDSSRPPAMSTSNMTSP*RRSRV 244
+SFTNT + R +S +S+++ +L + PS + P+ ++ +P +R+
Sbjct: 32 VSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKD-------TSRWNPLQRAAS 84
Query: 243 LIRPSLGTCRLRRDRHRPAPDPVPER 166
T LRR+R +P P V R
Sbjct: 85 AALDFAETALLRRERSKPLPKTVDPR 110
>At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase
family protein similar to SP|P31384 Glucose-repressible
alcohol dehydrogenase transcriptional effector (Carbon
catabolite repressor protein 4) {Saccharomyces
cerevisiae}; contains Pfam profile PF03372:
Endonuclease/Exonuclease/phosphatase family
Length = 602
Score = 29.1 bits (62), Expect = 1.4
Identities = 22/68 (32%), Positives = 29/68 (42%)
Frame = -2
Query: 222 TCRLRRDRHRPAPDPVPERRGNRSGHTSLSHLFFWS*SVDPIILVFLPSDSYNILILTGY 43
+C + R PAP P P R + SG HL + P+ + SYNIL T
Sbjct: 205 SCTILTSRVIPAPSPSPRRLISISGTDVTGHL---DSNGRPLSMGTFTVLSYNILSDTYA 261
Query: 42 VGAFYSFC 19
YS+C
Sbjct: 262 SSDIYSYC 269
>At3g63500.2 68416.m07153 expressed protein
Length = 1162
Score = 28.7 bits (61), Expect = 1.8
Identities = 20/70 (28%), Positives = 28/70 (40%)
Frame = -2
Query: 330 SIDTEPPDSSRPPAMSTSNMTSP*RRSRVLIRPSLGTCRLRRDRHRPAPDPVPERRGNRS 151
++D EPP+S RP + S S R R RR +R + P+ R +RS
Sbjct: 17 NVDGEPPNSDRPASSSHRGFFSGNNRDRGEDAAGFSRAFSRRRSNRDLDNHRPDARYHRS 76
Query: 150 GHTSLSHLFF 121
S F
Sbjct: 77 ESACFSRRAF 86
>At3g50180.1 68416.m05486 hypothetical protein
Length = 588
Score = 28.3 bits (60), Expect = 2.4
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = -2
Query: 318 EPPDSSRPPAMSTSNMTSP*RRSRVLIRPSLGTCRLRRDRHRPAPDPVPER 166
+PP PP P R R+L P RLR+ + P+P+P R
Sbjct: 28 QPPPPPPPPPPPPPPRLGPRLRLRLLPPPRQQLLRLRKQQPLVLPEPLPLR 78
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 28.3 bits (60), Expect = 2.4
Identities = 15/54 (27%), Positives = 25/54 (46%)
Frame = -2
Query: 423 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPDSSRPPAMSTSNMTSP 262
+S S R +I SL +K+ + PS+ ++PP +PP T + P
Sbjct: 321 MSQERAASASRDAAIISLIQKITGHTIQLPPSLSSQPPPPYQPPPAVTKRVAEP 374
>At2g44410.1 68415.m05523 expressed protein
Length = 413
Score = 27.9 bits (59), Expect = 3.1
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -2
Query: 315 PPDSSRPPAM-STSNMTSP*RRSRVLIRPSLGTCRLRRDRHRPAPDPVPERRGNRSGHTS 139
PP ++ P + TS++ +P + + T LRR+R RP+P + NRS + S
Sbjct: 287 PPYATFPGLLVDTSDIPAPFDDDAFDVDSFVDTTSLRRNRRRPSPAVRASYQRNRSNNAS 346
>At4g25000.1 68417.m03587 alpha-amylase, putative /
1,4-alpha-D-glucan glucanohydrolase, putative similar to
alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
GI:21670851; contains Pfam profile PF00128: Alpha
amylase, catalytic domain
Length = 423
Score = 27.5 bits (58), Expect = 4.1
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +3
Query: 129 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKY 224
D K YD N R L Q +E AGG + A+ +
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269
>At3g13760.1 68416.m01736 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 652
Score = 27.5 bits (58), Expect = 4.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 193 PVAISTQPTST*TRPDQNSTTTLWRSHIRCTHC 291
PV IST + T T W+ HI+CT C
Sbjct: 473 PVVISTSYSDTDEILCNACQGTCWQPHIKCTKC 505
>At4g38990.1 68417.m05524 glycosyl hydrolase family 9 protein
endo-1,4-beta-glucanase precursor - Fragariax ananassa,
PID:g3549291 A short intron was annotated between exons
4 and 5 to circumvent a frameshift. The frameshift may
be artificial due to a sequencing error, or
alternatively is genuine suggesting a truncated protein
or pseudogene.
Length = 495
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -2
Query: 423 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSP 331
+S T C KF+ S+ LA++ +LGD+P
Sbjct: 366 LSTTPLCLKFQPDSLRRLAKRQVDYILGDNP 396
>At2g31920.1 68415.m03899 expressed protein
Length = 585
Score = 26.6 bits (56), Expect = 7.2
Identities = 15/58 (25%), Positives = 25/58 (43%)
Frame = -2
Query: 402 SKFRMVSISSLAEKMQVLVLGDSPSIDTEPPDSSRPPAMSTSNMTSP*RRSRVLIRPS 229
SK+ S V+ G S+ + P D + P N +P RSR +++P+
Sbjct: 204 SKYFNCGTSPALRNKNVVKPGSPISMASSPKDGIKSPISKHLNCETPALRSRYVVKPA 261
>At5g12400.1 68418.m01458 PHD finger transcription factor, putative
similarity to predicted proteins, Arabidopsis thaliana
Length = 1595
Score = 26.2 bits (55), Expect = 9.6
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -2
Query: 384 SISSLAEKMQVLVLGDSPSIDTEPPDSSRPPA 289
SISSL E+ +V G + +I PP PP+
Sbjct: 373 SISSLTEEKTWIVDGKADNISALPPKPQLPPS 404
>At3g10595.1 68416.m01274 myb family transcription factor contains
similarity to Pfam profile: PF00249 Myb DNA binding
domain
Length = 183
Score = 26.2 bits (55), Expect = 9.6
Identities = 16/52 (30%), Positives = 22/52 (42%)
Frame = +1
Query: 187 SGPVAISTQPTST*TRPDQNSTTTLWRSHIRCTHCWRPAAVGRFGVDGRRIA 342
S VA + T + + T+W TH W + RFG D R+IA
Sbjct: 59 SSRVAFPNELTKDMAQSSYQAERTIWTKE---THEWFLIGLDRFGKDWRKIA 107
>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
subunit, putative contains 5 WD-40 repeats (PF00400);
similar to katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 1180
Score = 26.2 bits (55), Expect = 9.6
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
Frame = -2
Query: 363 KMQVLVLGDSPSI--DTEPPDSSRPPAMSTSNMTS 265
++ +VLG S+ DT P S+RP S +N+TS
Sbjct: 692 RVMPVVLGRDTSMATDTPPVTSTRPDRTSATNLTS 726
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,256,697
Number of Sequences: 28952
Number of extensions: 191378
Number of successful extensions: 750
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 676
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 749
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 685039728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -