Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20608
         (359 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit...    73   2e-14
SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4 |Schiz...    35   0.003
SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharom...    32   0.023
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|...    29   0.16 
SPBC4B4.09 |usp105|prp39|U1 snRNP-associated protein Usp105|Schi...    29   0.22 
SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces po...    29   0.22 
SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual         29   0.22 
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe...    27   0.88 
SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos...    27   1.2  
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    25   3.5  
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr...    24   6.2  
SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M...    24   6.2  
SPBC947.01 |||AAA family ATPase, unknown biological role|Schizos...    24   6.2  
SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH comple...    24   8.2  
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    24   8.2  
SPBC1604.02c |||PPR repeat protein|Schizosaccharomyces pombe|chr...    24   8.2  
SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ...    24   8.2  
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn...    24   8.2  

>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
           factor complex subunit Rna14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 733

 Score = 72.5 bits (170), Expect = 2e-14
 Identities = 32/84 (38%), Positives = 51/84 (60%), Gaps = 1/84 (1%)
 Frame = +3

Query: 6   YEKLITAFPTTGRYWKIYIEQEMKARNFERVEKLFQRCLMKILNIELWRLYLNYVKE-TK 182
           YE+++  FP   R W  YI  E+   +F  VE LF RCL+K+L+++LW LYL+Y++    
Sbjct: 94  YEQMLRPFPYVPRVWVDYISSELAFNDFHAVELLFSRCLVKVLSVDLWTLYLSYIRRINP 153

Query: 183 CMLPTYKEKMAQAYDFALDKIGLD 254
                 +  + QAY+F ++ IG+D
Sbjct: 154 DGEGQSRSTITQAYEFVINTIGVD 177



 Score = 31.9 bits (69), Expect = 0.031
 Identities = 12/31 (38%), Positives = 22/31 (70%)
 Frame = +2

Query: 266 PIWNDYVTFLKSVEAVGSYAENQKISAVQKI 358
           PIW+++V FL+S  A  ++ + QK+  V++I
Sbjct: 182 PIWSEFVDFLRSGPANSTWEQQQKLDHVRRI 212


>SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 674

 Score = 35.1 bits (77), Expect = 0.003
 Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
 Frame = +3

Query: 3   MYEKLITAFPTTGRYWKIYIEQEMKARNFERVEKLFQRCLMKILNIE-LWRLYLNYVKE 176
           ++E+ +    T    W  YIE EMK RN      LF R + ++  ++ LW  Y+ Y++E
Sbjct: 92  VFERALDVDSTYIPLWLKYIECEMKNRNINHARNLFDRAVTQLPRVDKLWYKYV-YMEE 149



 Score = 29.5 bits (63), Expect = 0.16
 Identities = 9/40 (22%), Positives = 22/40 (55%)
 Frame = +3

Query: 48  WKIYIEQEMKARNFERVEKLFQRCLMKILNIELWRLYLNY 167
           WK YI+ E +   + +   ++Q+ L    ++++W  + N+
Sbjct: 507 WKAYIDFEFEEMEYGKARSIYQQLLRTAPHVKVWISFANF 546



 Score = 27.1 bits (57), Expect = 0.88
 Identities = 11/53 (20%), Positives = 23/53 (43%)
 Frame = +3

Query: 3   MYEKLITAFPTTGRYWKIYIEQEMKARNFERVEKLFQRCLMKILNIELWRLYL 161
           ++++ +T  P   + W  Y+  E    N     ++F+R L    +   W  Y+
Sbjct: 126 LFDRAVTQLPRVDKLWYKYVYMEEMLGNITGCRQVFERWLKWEPDENCWMSYI 178



 Score = 25.8 bits (54), Expect = 2.0
 Identities = 12/40 (30%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = +3

Query: 45  YWKIYIEQEMKARNFERVEKLFQRCL-MKILNIELWRLYL 161
           +W  Y + E+  + F R   +F+R L +    I LW  Y+
Sbjct: 72  HWMRYGQWELDQKEFARARSVFERALDVDSTYIPLWLKYI 111


>SPCC1183.07 |||U3 snoRNP-associated protein Rrp5|Schizosaccharomyces
            pombe|chr 3|||Manual
          Length = 1690

 Score = 32.3 bits (70), Expect = 0.023
 Identities = 14/40 (35%), Positives = 24/40 (60%)
 Frame = +3

Query: 3    MYEKLITAFPTTGRYWKIYIEQEMKARNFERVEKLFQRCL 122
            ++E L++++P     W + I+ EMK  +   V +LFQR L
Sbjct: 1604 IFEGLLSSYPKRLDLWNVLIDMEMKQDDPSIVRRLFQRLL 1643


>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
           L7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 250

 Score = 29.5 bits (63), Expect = 0.16
 Identities = 22/66 (33%), Positives = 29/66 (43%)
 Frame = +2

Query: 14  VDNGIPDHRTLLENIYRTGDESEKF*KSRKALPEVSDEDIKHRTLEAVPELREGDEVHAT 193
           V  GIP+H+T+ E IY+ G     F K  K    +SD  I    L     L   D +H  
Sbjct: 141 VTYGIPNHKTVRELIYKRG-----FGKVNKQRIPLSDNAIIEAALGKYSILSVEDLIHEI 195

Query: 194 HLQGEN 211
           +  G N
Sbjct: 196 YTVGPN 201


>SPBC4B4.09 |usp105|prp39|U1 snRNP-associated protein
           Usp105|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 612

 Score = 29.1 bits (62), Expect = 0.22
 Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +3

Query: 48  WKIYIEQEMKARNFERVEKLFQRCLMK-ILNIELWRLYLNYV 170
           W+ Y++ E    +F+R+  L++RCL+   L  E W  Y  ++
Sbjct: 285 WRKYLDFEEVEGDFQRICHLYERCLITCALYDEFWFRYARWM 326


>SPBC1861.04c |||RNA-binding protein Prp24|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1014

 Score = 29.1 bits (62), Expect = 0.22
 Identities = 14/51 (27%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
 Frame = +3

Query: 27  FPTTGRYWKIYIEQEMK----ARNFERVEKLFQRCLMKILNIELWRLYLNY 167
           FP +   W  Y+  E K      ++ R++ LF   +   L+I++W +YL +
Sbjct: 73  FPLSEDLWVDYLLDECKNCRTLDDYVRIKTLFDLAVQDYLSIKIWCMYLEF 123


>SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 128

 Score = 29.1 bits (62), Expect = 0.22
 Identities = 13/49 (26%), Positives = 27/49 (55%)
 Frame = +3

Query: 54  IYIEQEMKARNFERVEKLFQRCLMKILNIELWRLYLNYVKETKCMLPTY 200
           +Y+++++      RV K   + + ++L I +  L+LNYV +  C +  Y
Sbjct: 8   LYVKKDLTYGECVRVAKEKYKIIHRLLYISIIFLFLNYVVDIVCYVKNY 56


>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 972

 Score = 27.1 bits (57), Expect = 0.88
 Identities = 18/84 (21%), Positives = 33/84 (39%)
 Frame = +3

Query: 6    YEKLITAFPTTGRYWKIYIEQEMKARNFERVEKLFQRCLMKILNIELWRLYLNYVKETKC 185
            +EK +T F      W++YI  E K R   + +    + L    N  +   +  +    + 
Sbjct: 797  FEKGVTLFSANTAIWEVYIFFESKLRQENKPKIRAMKILKSASNAVVTACWYLFYVAVQQ 856

Query: 186  MLPTYKEKMAQAYDFALDKIGLDS 257
            + PT  +   +  D  L+   L S
Sbjct: 857  IEPTNSQYFLRTLDITLNNEKLKS 880


>SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 790

 Score = 26.6 bits (56), Expect = 1.2
 Identities = 16/67 (23%), Positives = 32/67 (47%), Gaps = 11/67 (16%)
 Frame = +3

Query: 3   MYEKLITAFPTTGRYWKIYIEQEM----------KARNFERVEKLFQRCLMKILNIE-LW 149
           ++E+  +  P + + WK Y+E  +           A  F  V   F+R L+ +  +  +W
Sbjct: 53  LFERACSELPGSYKIWKSYLELRVAHVEHLNPYFHAEAFASVNDCFERSLILLHKMPVIW 112

Query: 150 RLYLNYV 170
           +LYL ++
Sbjct: 113 KLYLQFL 119



 Score = 24.6 bits (51), Expect = 4.7
 Identities = 13/57 (22%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
 Frame = +3

Query: 36  TGRYWKIYIEQEMKARNFERVEKLFQRCL-MKILNIELWRLYLNYVKETKCMLPTYK 203
           + + W  Y++ E      E   KL+ R   +KI   ++   Y N ++E      ++K
Sbjct: 479 SSKIWMYYLDLEESVGTIETTRKLYDRVFELKIATPQVVVNYANLLEENAYFEDSFK 535


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 25.0 bits (52), Expect = 3.5
 Identities = 10/29 (34%), Positives = 17/29 (58%)
 Frame = +3

Query: 48  WKIYIEQEMKARNFERVEKLFQRCLMKIL 134
           W +Y E  +  RN E +EK+ +   +K+L
Sbjct: 289 WAVY-ESAVSNRNLENIEKIIKALNIKVL 316


>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 1471

 Score = 24.2 bits (50), Expect = 6.2
 Identities = 9/26 (34%), Positives = 16/26 (61%)
 Frame = -2

Query: 130 IFIRHLWKSFSTLSKFLAFISCSIYI 53
           I I+  W+S+    ++L+ I C+I I
Sbjct: 805 ILIQAHWRSYIQRKRYLSLIKCAIVI 830


>SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 629

 Score = 24.2 bits (50), Expect = 6.2
 Identities = 8/17 (47%), Positives = 11/17 (64%)
 Frame = +3

Query: 300 VSKPLVHTPRTRRYPPF 350
           +S+ L+H     RYPPF
Sbjct: 70  LSRSLIHVQELSRYPPF 86


>SPBC947.01 |||AAA family ATPase, unknown biological
           role|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 660

 Score = 24.2 bits (50), Expect = 6.2
 Identities = 10/22 (45%), Positives = 13/22 (59%), Gaps = 1/22 (4%)
 Frame = +2

Query: 215 AGLR-LRPGQDRSGLHAYPIWN 277
           A LR +RP   + G+H Y  WN
Sbjct: 632 ASLRTIRPSVSQEGIHRYEEWN 653


>SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH complex
           subunit Tfb2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 447

 Score = 23.8 bits (49), Expect = 8.2
 Identities = 9/31 (29%), Positives = 20/31 (64%)
 Frame = +3

Query: 81  RNFERVEKLFQRCLMKILNIELWRLYLNYVK 173
           +N  R+ +   + L++ +N ++W L L+Y+K
Sbjct: 173 KNQLRITRAGFQFLLQDINAQIWTLLLDYLK 203


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 23.8 bits (49), Expect = 8.2
 Identities = 14/37 (37%), Positives = 18/37 (48%)
 Frame = -1

Query: 332 GSRRMNQRLRHSSETSRSRSILGKRGVQTYLVQGEVV 222
           GSRRMNQ L++  E   S   L        L QG ++
Sbjct: 744 GSRRMNQVLQYLEEQKTSSEPLPASYSTALLEQGRML 780


>SPBC1604.02c |||PPR repeat protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 697

 Score = 23.8 bits (49), Expect = 8.2
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +3

Query: 48  WKIYIEQEMKARNFERVEKL 107
           +++ IE  MK  NFE  EKL
Sbjct: 261 YRVLIESFMKFGNFEEAEKL 280


>SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1150

 Score = 23.8 bits (49), Expect = 8.2
 Identities = 15/46 (32%), Positives = 23/46 (50%)
 Frame = -2

Query: 193 GSMHFVSFT*FRYSLQSSMFNIFIRHLWKSFSTLSKFLAFISCSIY 56
           G      FT F  S +  + N+ I    K+FSTL + L F++ S +
Sbjct: 304 GDFTLFHFTLFLLS-KIILLNLKISDYEKAFSTLQQALLFLTSSFF 348


>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
           synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 534

 Score = 23.8 bits (49), Expect = 8.2
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +3

Query: 6   YEKLITAFPTTGRYWKIYIEQEMK 77
           YE+ ITAF     Y KI++E+  K
Sbjct: 453 YEQKITAFKHAHIYDKIFLEEARK 476


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,599,640
Number of Sequences: 5004
Number of extensions: 32239
Number of successful extensions: 135
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 110009772
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)