BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20606
(663 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 1e-44
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 101 9e-23
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 101 1e-22
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 94 1e-20
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 71 2e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 63 3e-11
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 54 1e-08
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 43 3e-05
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.085
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 30 0.34
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.45
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.4
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.2
SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 26 4.2
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.2
SPCC584.11c |||Svf1 family protein Svf1|Schizosaccharomyces pomb... 26 5.6
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 5.6
SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyce... 26 5.6
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 26 5.6
SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma... 25 9.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 174 bits (423), Expect = 1e-44
Identities = 78/88 (88%), Positives = 82/88 (93%)
Frame = +2
Query: 245 QKRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 424
++ LTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT
Sbjct: 61 KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMT 120
Query: 425 QIMFETFNTPAMYVAIQAVLSLYASGRT 508
QI+FETFN PA YVAIQAVLSLYASGRT
Sbjct: 121 QIIFETFNAPAFYVAIQAVLSLYASGRT 148
Score = 129 bits (311), Expect = 4e-31
Identities = 57/62 (91%), Positives = 61/62 (98%)
Frame = +3
Query: 69 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 248
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 249 RG 254
RG
Sbjct: 62 RG 63
Score = 66.9 bits (156), Expect = 2e-12
Identities = 29/35 (82%), Positives = 33/35 (94%)
Frame = +3
Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYS 659
IY+GYALPHAI+RLDLAGRDLT YLMKIL +RGY+
Sbjct: 165 IYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT 199
Score = 31.1 bits (67), Expect = 0.15
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = +1
Query: 511 GIVLDSGDGVSQTVP 555
GIVLDSGDGV+ TVP
Sbjct: 150 GIVLDSGDGVTHTVP 164
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 101 bits (242), Expect = 9e-23
Identities = 47/96 (48%), Positives = 61/96 (63%)
Frame = +2
Query: 260 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 439
+L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 440 TFNTPAMYVAIQAVLSLYASGRTPVSCWTPATVSPR 547
+FN +Y+A+QAVL+L AS WT + V+ R
Sbjct: 149 SFNCAGLYIAVQAVLALAAS-------WTSSKVTDR 177
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%)
Frame = +3
Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 236
+++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ +
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58
Score = 30.7 bits (66), Expect = 0.20
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +3
Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDR 650
+ +GY + +I + LAGRD+T ++ +L DR
Sbjct: 196 VAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +1
Query: 499 RSYPGIVLDSGDGVSQTVP 555
RS G V+DSGDGV+ +P
Sbjct: 177 RSLTGTVVDSGDGVTHIIP 195
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 101 bits (241), Expect = 1e-22
Identities = 42/85 (49%), Positives = 63/85 (74%)
Frame = +2
Query: 248 KRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 427
+ L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM +
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121
Query: 428 IMFETFNTPAMYVAIQAVLSLYASG 502
MFE + +YVAIQAVLSLYA G
Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQG 146
Score = 55.6 bits (128), Expect = 6e-09
Identities = 30/61 (49%), Positives = 39/61 (63%), Gaps = 2/61 (3%)
Frame = +3
Query: 75 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 248
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 249 R 251
R
Sbjct: 62 R 62
Score = 39.9 bits (89), Expect = 3e-04
Identities = 16/35 (45%), Positives = 24/35 (68%)
Frame = +3
Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYS 659
+Y+ L H + RLD+AGRD T YL+ +L +GY+
Sbjct: 165 VYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYA 199
Score = 27.1 bits (57), Expect = 2.4
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +1
Query: 511 GIVLDSGDGVSQTVP 555
G+V+DSGDGV+ VP
Sbjct: 150 GVVVDSGDGVTHIVP 164
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 94.3 bits (224), Expect = 1e-20
Identities = 42/85 (49%), Positives = 59/85 (69%), Gaps = 1/85 (1%)
Frame = +2
Query: 257 LTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIM 433
L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ +
Sbjct: 69 LKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYF 128
Query: 434 FETFNTPAMYVAIQAVLSLYASGRT 508
+ET N PA+ ++Q VL+LYAS RT
Sbjct: 129 YETLNVPALSFSLQPVLALYASART 153
Score = 73.3 bits (172), Expect = 3e-14
Identities = 32/56 (57%), Positives = 41/56 (73%)
Frame = +3
Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 254
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Score = 36.7 bits (81), Expect = 0.003
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +3
Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYSV 662
IYDG+++P AI + ++ GRD+T YL L G+ +
Sbjct: 170 IYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHEL 205
Score = 25.4 bits (53), Expect = 7.4
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +1
Query: 472 PSRALAVRVRSYPGIVLDSGDGVSQTVP 555
P AL R+ GIVL+ GDG++ +VP
Sbjct: 143 PVLALYASART-TGIVLECGDGLTHSVP 169
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 70.5 bits (165), Expect = 2e-13
Identities = 37/108 (34%), Positives = 53/108 (49%)
Frame = +2
Query: 182 PSGCDGRHGTERLVRRRRGTEQKRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE 361
PS C RL + + +K I +G V NWD +W + +L+ P
Sbjct: 38 PSYCGEFSDGRRLFGEEYIYKSNPGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPL 97
Query: 362 EHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGR 505
EHP+L+TE NP NR K + MFE+ PA Y+A Q + +ASG+
Sbjct: 98 EHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFASGK 145
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 72 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 173
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 63.3 bits (147), Expect = 3e-11
Identities = 27/74 (36%), Positives = 44/74 (59%)
Frame = +2
Query: 272 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 451
P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N
Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144
Query: 452 PAMYVAIQAVLSLY 493
P++ I + S Y
Sbjct: 145 PSVAYGIDGLFSFY 158
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +3
Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 182
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 54.4 bits (125), Expect = 1e-08
Identities = 22/76 (28%), Positives = 44/76 (57%)
Frame = +2
Query: 281 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 460
+GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA
Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133
Query: 461 YVAIQAVLSLYASGRT 508
Y+ QAV +A+ ++
Sbjct: 134 YLTKQAVCVAFANSKS 149
Score = 38.7 bits (86), Expect = 7e-04
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +3
Query: 72 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 242
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 43.2 bits (97), Expect = 3e-05
Identities = 25/81 (30%), Positives = 38/81 (46%), Gaps = 4/81 (4%)
Frame = +2
Query: 272 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 439
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 440 TFNTPAMYVAIQAVLSLYASG 502
P +A + ++ LYA G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIG 192
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.085
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 332 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 475
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 29.9 bits (64), Expect = 0.34
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = +3
Query: 132 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 254
G+ PRA F ++ P H G+++ M KD G+E S +G
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKG 540
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.5 bits (63), Expect = 0.45
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -3
Query: 229 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 68
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 27.1 bits (57), Expect = 2.4
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = +3
Query: 582 AILRLDLAGRDLTGYLMKILTDRGYSV 662
A+ R+D+ G+ LT YL ++++ R Y++
Sbjct: 182 AVKRVDVGGKLLTNYLKEVISYRKYNM 208
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 374 LLTEAPLNPKANREKMTQIMFETFN 448
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 658
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = -2
Query: 653 AAVGEDLHEVACEVATGQVQTQDGVRECVSVVD 555
AA G+ + +C++A+G Q+G + +S+V+
Sbjct: 402 AAAGKFFSQHSCDLASGNKSAQEGQNQFLSMVE 434
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 4.2
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 232 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 116
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPCC584.11c |||Svf1 family protein Svf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 380
Score = 25.8 bits (54), Expect = 5.6
Identities = 21/78 (26%), Positives = 29/78 (37%), Gaps = 9/78 (11%)
Frame = -1
Query: 597 PDAGWREGVRIRRRWYG------LGDTVAGVQHDTG---VRPDAYSESTAWMATYMAGVL 445
PD W E RI W G D V G R D +E +W+ ++ GV
Sbjct: 283 PDTEWHEPTRISYEWDGKDAETYTEDIHLSVDAPLGRRLQRIDVLAEIPSWLKGFVHGVS 342
Query: 444 NVSNMIWVIFSLLALGLR 391
I+ FS + L+
Sbjct: 343 GTKPFIYQYFSPVKFTLK 360
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 5.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 126 NRPCTYRSHCLLPTRQLP 73
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
>SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 698
Score = 25.8 bits (54), Expect = 5.6
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -1
Query: 273 GYLRVRNLFCSVPRL 229
GY +RN+ CSVPRL
Sbjct: 675 GYCIIRNVGCSVPRL 689
>SPBP35G2.05c |cki2||serine/threonine protein kinase
Cki2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 435
Score = 25.8 bits (54), Expect = 5.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -2
Query: 635 LHEVACEVATGQVQTQDGVRECVSVVDGTVWETPSPESSTI 513
L E+ +V T DGV + + + DG WE+ S S +
Sbjct: 274 LQELFDDVLRANGDTNDGVYDWMLLNDGKGWESSSSHFSVV 314
>SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 535
Score = 25.0 bits (52), Expect = 9.7
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 550 VPSTTDTHSLTPSCVW 597
VP TDT++LTP W
Sbjct: 30 VPENTDTYALTPLFYW 45
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,893,531
Number of Sequences: 5004
Number of extensions: 64172
Number of successful extensions: 216
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 213
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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