BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS20606 (663 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 174 1e-44 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 101 9e-23 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 101 1e-22 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 94 1e-20 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 71 2e-13 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 63 3e-11 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 54 1e-08 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 43 3e-05 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.085 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 30 0.34 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.45 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 27 2.4 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.2 SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces po... 26 4.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 4.2 SPCC584.11c |||Svf1 family protein Svf1|Schizosaccharomyces pomb... 26 5.6 SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 5.6 SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyce... 26 5.6 SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 26 5.6 SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma... 25 9.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 174 bits (423), Expect = 1e-44 Identities = 78/88 (88%), Positives = 82/88 (93%) Frame = +2 Query: 245 QKRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 424 ++ LTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT Sbjct: 61 KRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMT 120 Query: 425 QIMFETFNTPAMYVAIQAVLSLYASGRT 508 QI+FETFN PA YVAIQAVLSLYASGRT Sbjct: 121 QIIFETFNAPAFYVAIQAVLSLYASGRT 148 Score = 129 bits (311), Expect = 4e-31 Identities = 57/62 (91%), Positives = 61/62 (98%) Frame = +3 Query: 69 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 248 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 249 RG 254 RG Sbjct: 62 RG 63 Score = 66.9 bits (156), Expect = 2e-12 Identities = 29/35 (82%), Positives = 33/35 (94%) Frame = +3 Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYS 659 IY+GYALPHAI+RLDLAGRDLT YLMKIL +RGY+ Sbjct: 165 IYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT 199 Score = 31.1 bits (67), Expect = 0.15 Identities = 13/15 (86%), Positives = 14/15 (93%) Frame = +1 Query: 511 GIVLDSGDGVSQTVP 555 GIVLDSGDGV+ TVP Sbjct: 150 GIVLDSGDGVTHTVP 164 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 101 bits (242), Expect = 9e-23 Identities = 47/96 (48%), Positives = 61/96 (63%) Frame = +2 Query: 260 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFE 439 +L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148 Query: 440 TFNTPAMYVAIQAVLSLYASGRTPVSCWTPATVSPR 547 +FN +Y+A+QAVL+L AS WT + V+ R Sbjct: 149 SFNCAGLYIAVQAVLALAAS-------WTSSKVTDR 177 Score = 42.7 bits (96), Expect = 5e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +3 Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 236 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 Score = 30.7 bits (66), Expect = 0.20 Identities = 12/32 (37%), Positives = 21/32 (65%) Frame = +3 Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDR 650 + +GY + +I + LAGRD+T ++ +L DR Sbjct: 196 VAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDR 227 Score = 27.9 bits (59), Expect = 1.4 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = +1 Query: 499 RSYPGIVLDSGDGVSQTVP 555 RS G V+DSGDGV+ +P Sbjct: 177 RSLTGTVVDSGDGVTHIIP 195 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 101 bits (241), Expect = 1e-22 Identities = 42/85 (49%), Positives = 63/85 (74%) Frame = +2 Query: 248 KRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 427 + L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 428 IMFETFNTPAMYVAIQAVLSLYASG 502 MFE + +YVAIQAVLSLYA G Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQG 146 Score = 55.6 bits (128), Expect = 6e-09 Identities = 30/61 (49%), Positives = 39/61 (63%), Gaps = 2/61 (3%) Frame = +3 Query: 75 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 248 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 249 R 251 R Sbjct: 62 R 62 Score = 39.9 bits (89), Expect = 3e-04 Identities = 16/35 (45%), Positives = 24/35 (68%) Frame = +3 Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYS 659 +Y+ L H + RLD+AGRD T YL+ +L +GY+ Sbjct: 165 VYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYA 199 Score = 27.1 bits (57), Expect = 2.4 Identities = 10/15 (66%), Positives = 13/15 (86%) Frame = +1 Query: 511 GIVLDSGDGVSQTVP 555 G+V+DSGDGV+ VP Sbjct: 150 GVVVDSGDGVTHIVP 164 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 94.3 bits (224), Expect = 1e-20 Identities = 42/85 (49%), Positives = 59/85 (69%), Gaps = 1/85 (1%) Frame = +2 Query: 257 LTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQIM 433 L ++ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + Sbjct: 69 LKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYF 128 Query: 434 FETFNTPAMYVAIQAVLSLYASGRT 508 +ET N PA+ ++Q VL+LYAS RT Sbjct: 129 YETLNVPALSFSLQPVLALYASART 153 Score = 73.3 bits (172), Expect = 3e-14 Identities = 32/56 (57%), Positives = 41/56 (73%) Frame = +3 Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 254 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67 Score = 36.7 bits (81), Expect = 0.003 Identities = 15/36 (41%), Positives = 24/36 (66%) Frame = +3 Query: 555 IYDGYALPHAILRLDLAGRDLTGYLMKILTDRGYSV 662 IYDG+++P AI + ++ GRD+T YL L G+ + Sbjct: 170 IYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHEL 205 Score = 25.4 bits (53), Expect = 7.4 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = +1 Query: 472 PSRALAVRVRSYPGIVLDSGDGVSQTVP 555 P AL R+ GIVL+ GDG++ +VP Sbjct: 143 PVLALYASART-TGIVLECGDGLTHSVP 169 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 70.5 bits (165), Expect = 2e-13 Identities = 37/108 (34%), Positives = 53/108 (49%) Frame = +2 Query: 182 PSGCDGRHGTERLVRRRRGTEQKRFLTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPE 361 PS C RL + + +K I +G V NWD +W + +L+ P Sbjct: 38 PSYCGEFSDGRRLFGEEYIYKSNPGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPL 97 Query: 362 EHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGR 505 EHP+L+TE NP NR K + MFE+ PA Y+A Q + +ASG+ Sbjct: 98 EHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAAFASGK 145 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 72 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 173 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 63.3 bits (147), Expect = 3e-11 Identities = 27/74 (36%), Positives = 44/74 (59%) Frame = +2 Query: 272 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 451 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 452 PAMYVAIQAVLSLY 493 P++ I + S Y Sbjct: 145 PSVAYGIDGLFSFY 158 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 87 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 182 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 54.4 bits (125), Expect = 1e-08 Identities = 22/76 (28%), Positives = 44/76 (57%) Frame = +2 Query: 281 HGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAM 460 +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ + FE + PA Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAF 133 Query: 461 YVAIQAVLSLYASGRT 508 Y+ QAV +A+ ++ Sbjct: 134 YLTKQAVCVAFANSKS 149 Score = 38.7 bits (86), Expect = 7e-04 Identities = 20/57 (35%), Positives = 33/57 (57%) Frame = +3 Query: 72 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 242 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQ 58 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 43.2 bits (97), Expect = 3e-05 Identities = 25/81 (30%), Positives = 38/81 (46%), Gaps = 4/81 (4%) Frame = +2 Query: 272 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 439 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 440 TFNTPAMYVAIQAVLSLYASG 502 P +A + ++ LYA G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIG 192 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.085 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +2 Query: 332 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 475 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 29.9 bits (64), Expect = 0.34 Identities = 15/41 (36%), Positives = 23/41 (56%) Frame = +3 Query: 132 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 254 G+ PRA F ++ P H G+++ M KD G+E S +G Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKG 540 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.5 bits (63), Expect = 0.45 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -3 Query: 229 PTYESFCPM-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 68 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 27.1 bits (57), Expect = 2.4 Identities = 10/27 (37%), Positives = 20/27 (74%) Frame = +3 Query: 582 AILRLDLAGRDLTGYLMKILTDRGYSV 662 A+ R+D+ G+ LT YL ++++ R Y++ Sbjct: 182 AVKRVDVGGKLLTNYLKEVISYRKYNM 208 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.2 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +2 Query: 374 LLTEAPLNPKANREKMTQIMFETFN 448 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPCC1235.01 ||SPCC320.02c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 658 Score = 26.2 bits (55), Expect = 4.2 Identities = 10/33 (30%), Positives = 21/33 (63%) Frame = -2 Query: 653 AAVGEDLHEVACEVATGQVQTQDGVRECVSVVD 555 AA G+ + +C++A+G Q+G + +S+V+ Sbjct: 402 AAAGKFFSQHSCDLASGNKSAQEGQNQFLSMVE 434 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 4.2 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -3 Query: 232 SPTYESFC--PMPTITP*WRGLPTIEGNTARGASSPAKPAL 116 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPCC584.11c |||Svf1 family protein Svf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 25.8 bits (54), Expect = 5.6 Identities = 21/78 (26%), Positives = 29/78 (37%), Gaps = 9/78 (11%) Frame = -1 Query: 597 PDAGWREGVRIRRRWYG------LGDTVAGVQHDTG---VRPDAYSESTAWMATYMAGVL 445 PD W E RI W G D V G R D +E +W+ ++ GV Sbjct: 283 PDTEWHEPTRISYEWDGKDAETYTEDIHLSVDAPLGRRLQRIDVLAEIPSWLKGFVHGVS 342 Query: 444 NVSNMIWVIFSLLALGLR 391 I+ FS + L+ Sbjct: 343 GTKPFIYQYFSPVKFTLK 360 >SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 425 Score = 25.8 bits (54), Expect = 5.6 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = -1 Query: 126 NRPCTYRSHCLLPTRQLP 73 NRP YRSH + TR+ P Sbjct: 323 NRPSNYRSHSSMGTRRSP 340 >SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 698 Score = 25.8 bits (54), Expect = 5.6 Identities = 10/15 (66%), Positives = 12/15 (80%) Frame = -1 Query: 273 GYLRVRNLFCSVPRL 229 GY +RN+ CSVPRL Sbjct: 675 GYCIIRNVGCSVPRL 689 >SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizosaccharomyces pombe|chr 2|||Manual Length = 435 Score = 25.8 bits (54), Expect = 5.6 Identities = 13/41 (31%), Positives = 20/41 (48%) Frame = -2 Query: 635 LHEVACEVATGQVQTQDGVRECVSVVDGTVWETPSPESSTI 513 L E+ +V T DGV + + + DG WE+ S S + Sbjct: 274 LQELFDDVLRANGDTNDGVYDWMLLNDGKGWESSSSHFSVV 314 >SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 535 Score = 25.0 bits (52), Expect = 9.7 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = +1 Query: 550 VPSTTDTHSLTPSCVW 597 VP TDT++LTP W Sbjct: 30 VPENTDTYALTPLFYW 45 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,893,531 Number of Sequences: 5004 Number of extensions: 64172 Number of successful extensions: 216 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 184 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 213 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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