Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20599
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 126 3e-30
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 84 2e-17
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 83 5e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 71 1e-13
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 30 0.37
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.48
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.0
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.5
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 26 6.0
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.9
SPAC9G1.05 |||actin cortical patch component Aip1 |Schizosacchar... 25 7.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 126 bits (304), Expect = 3e-30
Identities = 53/69 (76%), Positives = 63/69 (91%)
Frame = +2
Query: 254 LDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESE 433
+DLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E
Sbjct: 66 VDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAE 125
Query: 434 SCDCLQGFQ 460
+CD LQGFQ
Sbjct: 126 ACDALQGFQ 134
Score = 115 bits (277), Expect = 6e-27
Identities = 48/70 (68%), Positives = 57/70 (81%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 239
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 240 PRAILSTWSP 269
PRA+L P
Sbjct: 61 PRAVLVDLEP 70
Score = 107 bits (257), Expect = 2e-24
Identities = 50/67 (74%), Positives = 57/67 (85%)
Frame = +1
Query: 499 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 678
G L+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL
Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEAL 207
Query: 679 YDICFRT 699
I T
Sbjct: 208 SSIFANT 214
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 83.8 bits (198), Expect = 2e-17
Identities = 33/68 (48%), Positives = 50/68 (73%)
Frame = +2
Query: 254 LDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESE 433
+DLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ ++
Sbjct: 72 VDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMAD 131
Query: 434 SCDCLQGF 457
+C LQGF
Sbjct: 132 NCSGLQGF 139
Score = 68.9 bits (161), Expect = 6e-13
Identities = 30/66 (45%), Positives = 45/66 (68%)
Frame = +1
Query: 499 GHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL 678
G L+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D T+ +DNEA
Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEAC 213
Query: 679 YDICFR 696
YDIC R
Sbjct: 214 YDICRR 219
Score = 50.8 bits (116), Expect = 2e-07
Identities = 26/76 (34%), Positives = 38/76 (50%), Gaps = 6/76 (7%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 221
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 222 SGGKYVPRAILSTWSP 269
GK+VPR+I P
Sbjct: 61 GQGKFVPRSIYVDLEP 76
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 82.6 bits (195), Expect = 5e-17
Identities = 33/68 (48%), Positives = 48/68 (70%)
Frame = +2
Query: 254 LDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESE 433
+DLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ ++
Sbjct: 68 VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127
Query: 434 SCDCLQGF 457
+C LQGF
Sbjct: 128 NCSGLQGF 135
Score = 65.3 bits (152), Expect = 8e-12
Identities = 28/67 (41%), Positives = 44/67 (65%)
Frame = +1
Query: 496 YGHPLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 675
+G L+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D T+ +DNE+
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNES 208
Query: 676 LYDICFR 696
YDIC R
Sbjct: 209 CYDICRR 215
Score = 56.8 bits (131), Expect = 3e-09
Identities = 28/72 (38%), Positives = 39/72 (54%), Gaps = 2/72 (2%)
Frame = +3
Query: 60 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 233
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 234 YVPRAILSTWSP 269
YVPR+I P
Sbjct: 61 YVPRSIYVDLEP 72
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 71.3 bits (167), Expect = 1e-13
Identities = 31/69 (44%), Positives = 46/69 (66%)
Frame = +3
Query: 63 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 242
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 243 RAILSTWSP 269
RAIL P
Sbjct: 63 RAILIDLEP 71
Score = 59.7 bits (138), Expect = 4e-10
Identities = 23/70 (32%), Positives = 46/70 (65%), Gaps = 2/70 (2%)
Frame = +2
Query: 254 LDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKE 427
+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E
Sbjct: 67 IDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDRE 125
Query: 428 SESCDCLQGF 457
++ D L+GF
Sbjct: 126 ADGSDSLEGF 135
Score = 58.0 bits (134), Expect = 1e-09
Identities = 28/64 (43%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
Frame = +1
Query: 499 GHPLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEA 675
G L+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D +DN A
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAA 209
Query: 676 LYDI 687
L I
Sbjct: 210 LAHI 213
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 29.9 bits (64), Expect = 0.37
Identities = 24/69 (34%), Positives = 38/69 (55%), Gaps = 11/69 (15%)
Frame = +1
Query: 493 RYGHPLISKIREEYP-DRIMNTYSVVP--SPKVSDTVVE---PYN--ATLSVHQL---VE 639
+Y H + SK++ EYP D+++ SV+ PK VVE P N A L+ H + ++
Sbjct: 392 QYAHVVASKLQREYPRDKVLYYSSVLTEFDPKGIQEVVESLRPNNFFAILAAHSIEKGLD 451
Query: 640 NTDETYCID 666
N ++ Y ID
Sbjct: 452 NKEKFYGID 460
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 29.5 bits (63), Expect = 0.48
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +3
Query: 459 NLHIPSVEAPGPVWAPSHLKDP*RVPRQNHEHILSSPLAQS 581
N+H PS A + +++P +P+QNHEH SPL +S
Sbjct: 623 NIHHPSANLLDASAALNPVQNPLLMPQQNHEH---SPLVRS 660
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 2.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 362 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 451
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 99 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 200
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 293 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 394
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +1
Query: 43 KITYTK*GKSFIYRPANVV 99
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/43 (37%), Positives = 21/43 (48%)
Frame = +2
Query: 227 RQVRAPRHPLDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGN 355
R+V AP+H GT RS +FR ++ FGQ GN
Sbjct: 486 REVFAPKHARR-RLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 314 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 445
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC9G1.05 |||actin cortical patch component Aip1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 595
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3
Query: 417 SAKNQNLAIAYRASNLHIPSVEAP 488
+AK+ +LA A +N+HI SVE P
Sbjct: 531 NAKSTHLATASLDTNIHIYSVERP 554
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 351 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 256
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,736,141
Number of Sequences: 5004
Number of extensions: 55456
Number of successful extensions: 178
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -