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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20595
         (668 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   121   1e-28
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   121   1e-28
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   121   1e-28
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    40   4e-04
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.086
SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|ch...    27   3.2  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   7.5  
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    25   9.9  
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    25   9.9  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  121 bits (291), Expect = 1e-28
 Identities = 53/72 (73%), Positives = 60/72 (83%)
 Frame = +1

Query: 37  KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
           K  PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E 
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387

Query: 217 NPKSIKSGDAAI 252
           +PK +KSGDA I
Sbjct: 388 SPKFVKSGDACI 399



 Score = 80.2 bits (189), Expect = 2e-16
 Identities = 53/127 (41%), Positives = 66/127 (51%), Gaps = 4/127 (3%)
 Frame = +3

Query: 3   ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*PSWSNLKRLHTSIGLPHCPHCLQICR 170
           ++RRG V GDSKN+P  GC  F +     +H    S      L        C     I +
Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEK 376

Query: 171 NQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 350
             R+S           PK  +        +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 377 IDRRSGKKIE----ESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432

Query: 351 VGVIKAV 371
           VGVIKAV
Sbjct: 433 VGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  121 bits (291), Expect = 1e-28
 Identities = 53/72 (73%), Positives = 60/72 (83%)
 Frame = +1

Query: 37  KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
           K  PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E 
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387

Query: 217 NPKSIKSGDAAI 252
           +PK +KSGDA I
Sbjct: 388 SPKFVKSGDACI 399



 Score = 80.2 bits (189), Expect = 2e-16
 Identities = 53/127 (41%), Positives = 66/127 (51%), Gaps = 4/127 (3%)
 Frame = +3

Query: 3   ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*PSWSNLKRLHTSIGLPHCPHCLQICR 170
           ++RRG V GDSKN+P  GC  F +     +H    S      L        C     I +
Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEK 376

Query: 171 NQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 350
             R+S           PK  +        +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 377 IDRRSGKKIE----ESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432

Query: 351 VGVIKAV 371
           VGVIKAV
Sbjct: 433 VGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  121 bits (291), Expect = 1e-28
 Identities = 53/72 (73%), Positives = 60/72 (83%)
 Frame = +1

Query: 37  KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
           K  PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK  E 
Sbjct: 328 KNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKKIEE 387

Query: 217 NPKSIKSGDAAI 252
           +PK +KSGDA I
Sbjct: 388 SPKFVKSGDACI 399



 Score = 80.2 bits (189), Expect = 2e-16
 Identities = 53/127 (41%), Positives = 66/127 (51%), Gaps = 4/127 (3%)
 Frame = +3

Query: 3   ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*PSWSNLKRLHTSIGLPHCPHCLQICR 170
           ++RRG V GDSKN+P  GC  F +     +H    S      L        C     I +
Sbjct: 317 DIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEK 376

Query: 171 NQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 350
             R+S           PK  +        +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 377 IDRRSGKKIE----ESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432

Query: 351 VGVIKAV 371
           VGVIKAV
Sbjct: 433 VGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 19/61 (31%), Positives = 31/61 (50%)
 Frame = +1

Query: 25  LVTPKTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 204
           ++T    P      F AQ+ +L  P  ++ GY+ V+  HTA     FA++  K+D+   K
Sbjct: 546 VLTSTKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDKTNRK 605

Query: 205 S 207
           S
Sbjct: 606 S 606



 Score = 37.9 bits (84), Expect = 0.001
 Identities = 16/35 (45%), Positives = 26/35 (74%), Gaps = 1/35 (2%)
 Frame = +3

Query: 273 PLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 374
           P+C+E F+++  +GRF +RD   TVAVG V+K ++
Sbjct: 628 PVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.086
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +3

Query: 273 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 365
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPBC691.01 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 312

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = +1

Query: 463 FSYNCDTSFTERCFEEKRATNSFLFYIFYKACNVTL 570
           F + C     + CF  ++    FLFY F  AC V +
Sbjct: 139 FDHYCSFVGKDVCFSNQKFFYQFLFYGFSAACMVLI 174


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -1

Query: 251 MAASPDLMDFGLTSVDLPVRRSTFS 177
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 10/37 (27%), Positives = 19/37 (51%)
 Frame = -2

Query: 430 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 320
           P  + ++   T+PP  S+  T  + PT ++   + TA
Sbjct: 73  PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -2

Query: 403 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 281
           + L P +S  +    TP+AT    S+  + P GG    D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,684,125
Number of Sequences: 5004
Number of extensions: 53859
Number of successful extensions: 158
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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