Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS20595
(668 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 111 3e-25
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 111 3e-25
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 111 3e-25
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 111 3e-25
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 81 5e-16
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 41 6e-04
At5g51750.1 68418.m06417 subtilase family protein similar to sub... 34 0.074
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 33 0.23
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 30 1.6
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 3.7
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 28 4.9
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 6.5
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 111 bits (268), Expect = 3e-25
Identities = 48/73 (65%), Positives = 59/73 (80%)
Frame = +1
Query: 37 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
K P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 217 NPKSIKSGDAAIV 255
PK +K+GDA +V
Sbjct: 378 EPKFLKNGDAGMV 390
Score = 74.1 bits (174), Expect = 8e-14
Identities = 51/131 (38%), Positives = 71/131 (54%), Gaps = 7/131 (5%)
Frame = +3
Query: 3 ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*--PSWSNLKRLHTS-IGLPHCPHCLQ 161
+L+RGYVA +SK++P G F S +H ++ + HTS I + +
Sbjct: 307 DLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTK 366
Query: 162 ICRNQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 341
I R K +PK + + P+KP+ VE+F E+PPLGRFAVRDMRQ
Sbjct: 367 IDRRSGKEIEK-------EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQ 419
Query: 342 TVAVGVIKAVN 374
TVAVGVIK+V+
Sbjct: 420 TVAVGVIKSVD 430
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 111 bits (268), Expect = 3e-25
Identities = 48/73 (65%), Positives = 59/73 (80%)
Frame = +1
Query: 37 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
K P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 217 NPKSIKSGDAAIV 255
PK +K+GDA +V
Sbjct: 378 EPKFLKNGDAGMV 390
Score = 74.1 bits (174), Expect = 8e-14
Identities = 51/131 (38%), Positives = 71/131 (54%), Gaps = 7/131 (5%)
Frame = +3
Query: 3 ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*--PSWSNLKRLHTS-IGLPHCPHCLQ 161
+L+RGYVA +SK++P G F S +H ++ + HTS I + +
Sbjct: 307 DLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTK 366
Query: 162 ICRNQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 341
I R K +PK + + P+KP+ VE+F E+PPLGRFAVRDMRQ
Sbjct: 367 IDRRSGKEIEK-------EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQ 419
Query: 342 TVAVGVIKAVN 374
TVAVGVIK+V+
Sbjct: 420 TVAVGVIKSVD 430
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 111 bits (268), Expect = 3e-25
Identities = 48/73 (65%), Positives = 59/73 (80%)
Frame = +1
Query: 37 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
K P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 217 NPKSIKSGDAAIV 255
PK +K+GDA +V
Sbjct: 378 EPKFLKNGDAGMV 390
Score = 74.1 bits (174), Expect = 8e-14
Identities = 51/131 (38%), Positives = 71/131 (54%), Gaps = 7/131 (5%)
Frame = +3
Query: 3 ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*--PSWSNLKRLHTS-IGLPHCPHCLQ 161
+L+RGYVA +SK++P G F S +H ++ + HTS I + +
Sbjct: 307 DLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTK 366
Query: 162 ICRNQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 341
I R K +PK + + P+KP+ VE+F E+PPLGRFAVRDMRQ
Sbjct: 367 IDRRSGKEIEK-------EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQ 419
Query: 342 TVAVGVIKAVN 374
TVAVGVIK+V+
Sbjct: 420 TVAVGVIKSVD 430
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 111 bits (268), Expect = 3e-25
Identities = 48/73 (65%), Positives = 59/73 (80%)
Frame = +1
Query: 37 KTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEV 216
K P KGAA+FT+QVI++NHPGQI NGY PVLDCHT+HIA KF+EI K+DRR+GK E
Sbjct: 318 KDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTKIDRRSGKEIEK 377
Query: 217 NPKSIKSGDAAIV 255
PK +K+GDA +V
Sbjct: 378 EPKFLKNGDAGMV 390
Score = 74.1 bits (174), Expect = 8e-14
Identities = 51/131 (38%), Positives = 71/131 (54%), Gaps = 7/131 (5%)
Frame = +3
Query: 3 ELRRGYVAGDSKNNPT*GCCRFYSS----SHCA*--PSWSNLKRLHTS-IGLPHCPHCLQ 161
+L+RGYVA +SK++P G F S +H ++ + HTS I + +
Sbjct: 307 DLKRGYVASNSKDDPAKGAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSEILTK 366
Query: 162 ICRNQRKS*PSYW*IY*SQPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQ 341
I R K +PK + + P+KP+ VE+F E+PPLGRFAVRDMRQ
Sbjct: 367 IDRRSGKEIEK-------EPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGRFAVRDMRQ 419
Query: 342 TVAVGVIKAVN 374
TVAVGVIK+V+
Sbjct: 420 TVAVGVIKSVD 430
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 81.4 bits (192), Expect = 5e-16
Identities = 35/56 (62%), Positives = 43/56 (76%)
Frame = +1
Query: 88 LNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIV 255
+NH GQI NGYTPVLDCHT+HIA KF+EI K+D RTG E PK +K+ +AAI+
Sbjct: 1 MNHLGQIKNGYTPVLDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAII 56
Score = 65.3 bits (152), Expect = 3e-11
Identities = 28/52 (53%), Positives = 39/52 (75%)
Frame = +3
Query: 216 QPKIHQVWRCSHCNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 371
+PK + + N+ P+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V
Sbjct: 44 EPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSV 95
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 41.1 bits (92), Expect = 6e-04
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +3
Query: 252 CNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 362
C + + +C+E F +FP LGRF +R +T+AVG +
Sbjct: 486 CRIQVTNSICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = +1
Query: 46 PPKGAADFTAQVIVLN--HPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVN 219
P +F AQ+ +L + GY +L H C+ E+K ++D +T K +
Sbjct: 415 PVPAVTEFVAQLQILELLDNAIFTAGYKAILHIHAVVEECEIIELKSQIDLKTRKPMKKK 474
Query: 220 PKSIKSGDAAI 252
+K+G A +
Sbjct: 475 VLFVKNGAAVV 485
>At5g51750.1 68418.m06417 subtilase family protein similar to
subtilisin-like protease GI:3687307 from [Lycopersicon
esculentum]
Length = 780
Score = 34.3 bits (75), Expect = 0.074
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 2/83 (2%)
Frame = -2
Query: 487 MKYRSCMKNCAVNSSSYFLPLVAFSAALVTLPPPASLKLTALMTPTATVCLMSR-TAKRP 311
M R + N + SSY + + F A VT+ P + + TV +R KRP
Sbjct: 695 MTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRP 754
Query: 310 R-GGNSWKDSTHRGLEGTKLQWL 245
GG WK +TH+ + WL
Sbjct: 755 EFGGLVWKSTTHKVRSPVIITWL 777
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +3
Query: 273 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 371
P+CVE+F E LGR +R +TVA+G + +
Sbjct: 631 PVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 29.9 bits (64), Expect = 1.6
Identities = 19/77 (24%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +1
Query: 22 LLVTPKTTPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTA--HIACKFAEIKEKVDRR 195
++V PK P D+ ++ L P + + G T + +A F+E+ + VD+R
Sbjct: 455 VVVEPKLVQPTFVLDYPIEISPLAKPHRGNAGLTERFELFICGREMANAFSELTDPVDQR 514
Query: 196 TGKSTEVNPKSIKSGDA 246
T +V + K +A
Sbjct: 515 TRLEEQVRQHNAKRAEA 531
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 3.7
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +1
Query: 541 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 639
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 28.3 bits (60), Expect = 4.9
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +1
Query: 160 KFAEIKEKVDRRTGKSTEVNPKSI 231
+F + KEK+DR GK E+N K +
Sbjct: 258 RFPKPKEKIDREGGKPLEINVKKL 281
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 6.5
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = +1
Query: 541 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 639
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,081,628
Number of Sequences: 28952
Number of extensions: 287487
Number of successful extensions: 783
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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