SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS20591
         (698 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...   108   3e-24
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...   104   7e-23
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...   104   7e-23
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...   104   7e-23
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...   103   1e-22
At3g11480.1 68416.m01400 S-adenosyl-L-methionine:carboxyl methyl...    27   9.0  
At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu...    27   9.0  

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score =  108 bits (260), Expect = 3e-24
 Identities = 54/98 (55%), Positives = 69/98 (70%), Gaps = 2/98 (2%)
 Frame = +1

Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
           GEIT+KANVDY+++VR+T + IG+  +  G D   C V++ ++QQSP+IA GVH +  + 
Sbjct: 55  GEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKK 114

Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHTNSIRKL 543
            EEVGAGDQG MFGYATDET E MPL   L T    KL
Sbjct: 115 PEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKL 152



 Score = 91.9 bits (218), Expect = 4e-19
 Identities = 44/70 (62%), Positives = 53/70 (75%), Gaps = 4/70 (5%)
 Frame = +2

Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVL----LLAKSHP 271
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV+    +  K++ 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63

Query: 272 KLTWIIKKLC 301
               I++K C
Sbjct: 64  DYEQIVRKTC 73



 Score = 77.0 bits (181), Expect = 1e-14
 Identities = 35/60 (58%), Positives = 42/60 (70%)
 Frame = +3

Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
           VLA KL  K+ E+R+NG   W RPD KTQVT EY+   GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAMVPVRVHTVLISTQHDETVT 202


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score =  104 bits (249), Expect = 7e-23
 Identities = 49/92 (53%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
 Frame = +1

Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
           GEIT+KA +DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  + 
Sbjct: 55  GEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKR 114

Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
            E++GAGDQG MFGYATDET E MPL   L T
Sbjct: 115 PEDIGAGDQGHMFGYATDETPELMPLSHVLAT 146



 Score = 93.5 bits (222), Expect = 1e-19
 Identities = 43/65 (66%), Positives = 51/65 (78%)
 Frame = +2

Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++  +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63

Query: 284 IIKKL 298
             +K+
Sbjct: 64  DYEKI 68



 Score = 73.3 bits (172), Expect = 1e-13
 Identities = 33/60 (55%), Positives = 41/60 (68%)
 Frame = +3

Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
           VLA K+  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVT 202


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  104 bits (249), Expect = 7e-23
 Identities = 51/92 (55%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
 Frame = +1

Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
           GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  + 
Sbjct: 55  GEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKC 114

Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
            EE+GAGDQG MFGYATDET E MPL   L T
Sbjct: 115 PEEIGAGDQGHMFGYATDETPELMPLSHVLAT 146



 Score = 93.5 bits (222), Expect = 1e-19
 Identities = 43/65 (66%), Positives = 51/65 (78%)
 Frame = +2

Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++  +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 284 IIKKL 298
             +K+
Sbjct: 64  DYEKI 68



 Score = 72.9 bits (171), Expect = 2e-13
 Identities = 34/60 (56%), Positives = 41/60 (68%)
 Frame = +3

Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
           VLA KL  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVT 202


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  104 bits (249), Expect = 7e-23
 Identities = 51/92 (55%), Positives = 65/92 (70%), Gaps = 2/92 (2%)
 Frame = +1

Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
           GEIT+KA VDY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  + 
Sbjct: 55  GEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKC 114

Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHT 525
            EE+GAGDQG MFGYATDET E MPL   L T
Sbjct: 115 PEEIGAGDQGHMFGYATDETPELMPLSHVLAT 146



 Score = 93.5 bits (222), Expect = 1e-19
 Identities = 43/65 (66%), Positives = 51/65 (78%)
 Frame = +2

Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAKSHPKLTW 283
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++  +   K T 
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 284 IIKKL 298
             +K+
Sbjct: 64  DYEKI 68



 Score = 72.9 bits (171), Expect = 2e-13
 Identities = 34/60 (56%), Positives = 41/60 (68%)
 Frame = +3

Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
           VLA KL  ++ E+R+NG   W RPD KTQVT EY    GA VP RVHTV++S QH E +T
Sbjct: 143 VLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAMVPIRVHTVLISTQHDETVT 202


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score =  103 bits (247), Expect = 1e-22
 Identities = 51/98 (52%), Positives = 67/98 (68%), Gaps = 2/98 (2%)
 Frame = +1

Query: 256 GEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RN 429
           GEIT+ A VDY+K+VR T + IG+  +  G D   C+V++ ++QQSP+IA GVH +  + 
Sbjct: 55  GEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKK 114

Query: 430 DEEVGAGDQGLMFGYATDETEECMPLM*CLHTNSIRKL 543
            E++GAGDQG MFGYATDET E MPL   L T    KL
Sbjct: 115 PEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKL 152



 Score = 90.2 bits (214), Expect = 1e-18
 Identities = 40/53 (75%), Positives = 46/53 (86%)
 Frame = +2

Query: 104 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLLAK 262
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++  +
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGE 56



 Score = 72.9 bits (171), Expect = 2e-13
 Identities = 34/60 (56%), Positives = 41/60 (68%)
 Frame = +3

Query: 513 VLAHKLNQKIAELRRNGEFWWARPDSKTQVTCEYVFAGGATVPQRVHTVVVSLQHSEKIT 692
           VLA KL  K+ E+R+N    W RPD KTQVT EY   GGA +P RVHTV++S QH E +T
Sbjct: 143 VLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAMIPIRVHTVLISTQHDETVT 202


>At3g11480.1 68416.m01400 S-adenosyl-L-methionine:carboxyl
           methyltransferase family protein similar to SAM:benzoic
           acid carboxyl methyltransferase
           (BAMT)[GI:9789277][Antirrhinum majus] and to
           SAM:salicylic acid carboxyl methyltransferase
           (SAMT)[GI:6002712][Clarkia breweri]
          Length = 379

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 14/46 (30%), Positives = 28/46 (60%)
 Frame = -3

Query: 357 LVIKAFGRIIITNMFDRFAHNFLIIHVSFGCDFANKSTIPVLVMVS 220
           ++I  FG  II  +FD++A+     HV+   +  NK+T+ ++V ++
Sbjct: 337 MLIAHFGEEIIDTLFDKYAY-----HVTQHANCRNKTTVSLVVSLT 377


>At1g05055.1 68414.m00506 basic transcription factor 2, 44kD
           subunit-related contains weak similarity to
           Swiss-Prot:Q13888 TFIIH basal transcription factor
           complex p44 subunit (Basic transcription factor 2 44 kDa
           subunit, BTF2-p44, General transcription factor IIH
           polypeptide 2) [Homo sapiens]
          Length = 421

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 12/28 (42%), Positives = 17/28 (60%)
 Frame = -3

Query: 525 CVQALHQRHAFFCLICCITEHQALVSCP 442
           CV     +H +FCL C I  H++L +CP
Sbjct: 379 CVTCRKCKH-YFCLDCDIYIHESLHNCP 405


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,092,812
Number of Sequences: 28952
Number of extensions: 394262
Number of successful extensions: 1121
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1072
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1115
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -